BGC0001363 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001363 (Ralstonia pseudosolanacearum GMI1000)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	43,202	ralsolamycin	NRPS:Type I+PKS	100%

BGC0001363 - Region 1 - NRPS,T1PKS

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Location: 1 - 43,202 nt. (total: 43,202 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 43,203
Label		BGC0001363

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001363	2.00	NRP, Polyketide	ralsolamycin	Ralstonia solanacearum GMI1000
BGC0002071	0.94	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001133	0.86	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001844	0.76	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001758	0.75	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001131	0.73	NRP	ambactin	Xenorhabdus miraniensis
BGC0002135	0.72	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.70	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001833	0.70	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001135	0.68	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001363	1.00	NRP, Polyketide	ralsolamycin	Ralstonia solanacearum GMI1000
BGC0002071	0.77	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001133	0.74	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001844	0.70	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001758	0.70	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001131	0.69	NRP	ambactin	Xenorhabdus miraniensis
BGC0002135	0.69	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.68	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001833	0.68	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001135	0.67	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 43202:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - Thr - Tyr - Ser - X) + (Val - Ser - Gly - Ala - bAla)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

RSp0641

Search NORINE for peptide: strict or relaxed

CAL_domain (45..486): pk

minowa: NH2

Prediction, score:

NH2: 125.2
Acetyl-CoA: 40.0
fatty_acid: 28.8
AHBA: 8.4
shikimic_acid: 0.0

PKS_AT (1229..1514): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.5
inactive: 116.3
Methoxymalonyl-CoA: 73.7
Methylmalonyl-CoA: 58.2
Propionyl-CoA: 43.1
Ethylmalonyl-CoA: 32.2
Isobutyryl-CoA: 25.8
Benzoyl-CoA: 23.3
2-Methylbutyryl-CoA: 19.9
CHC-CoA: 19.7
fatty_acid: 19.2
Acetyl-CoA: 17.1
trans-1,2-CPDA: 13.4
3-Methylbutyryl-CoA: 5.6

AMP-binding (3156..3560): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4213..4603): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTIAAVCK
Nearest Stachelhaus code(s):: Tyr DASTIAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5259..5657): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHLSLIDK
Nearest Stachelhaus code(s):: Ser DVWHLSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6311..6708): X

nrpys: Hse

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLKNVGSDVK
Nearest Stachelhaus code(s):: Hse DLKNVGSDVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

RSp0642

Search NORINE for peptide: strict or relaxed

AMP-binding (566..962): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DALWMGGTFK
Nearest Stachelhaus code(s):: Val DALWMGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1624..2022): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHLSLIDK
Nearest Stachelhaus code(s):: Ser DVWHLSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2704..3100): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code(s):: Gly DILQLGVIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3756..4144): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4800..5198): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: VDTVVSFGDK
Nearest Stachelhaus code(s):: bAla VDTVVSFGDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)