BGC0001371 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001371 (Aspergillus nidulans NRRL 8112)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,betalactone	1	56,631	High	echinocandin B	NRPS:Type I

BGC0001371 - Region 1 - NRPS,betalactone

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Location: 1 - 56,631 nt. (total: 56,631 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
betalactone: (HMGL-like and AMP-binding)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 56,632
Label		BGC0001371

Tailoring functions

Oxidoreductases: oxygenase

aniF2: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

aniH: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Ligases: ligase synthase

aniA: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

aniE: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

aniI: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001371.4	1.78	NRPS	echinocandin B	Aspergillus nidulans NRRL 8112
BGC0002611.2	1.15	NRPS	mulundocandin	Aspergillus mulundensis
BGC0002135.2	0.81	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.77	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001166.5	0.77	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.77	NRPS	AM-toxin	Alternaria alternata
BGC0001758.4	0.75	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001240.4	0.72	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001371.4	0.98	NRPS	echinocandin B	Aspergillus nidulans NRRL 8112
BGC0002611.2	0.84	NRPS	mulundocandin	Aspergillus mulundensis
BGC0002135.2	0.72	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.71	NRPS	AM-toxin	Alternaria alternata
BGC0001758.4	0.70	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001240.4	0.69	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 56072:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Orn - Thr - Pro - X - Thr - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

aniA: Orn - Thr - Pro - X - Thr - X

Search NORINE for peptide: strict or relaxed

AMP-binding (593..991): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVMELSSITK
Nearest Stachelhaus code(s):: Orn DVMELSSITK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1671..2073): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQTAVAIHK
Nearest Stachelhaus code(s):: Thr DAQTAVAIHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2781..3175): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVSSATTVCK
Nearest Stachelhaus code(s):: Pro DVSSATTVCK (100% 8Å match); 4OH-Pro DVSSATTVCK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3869..4272): X

nrpys: Hty

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGEAVGCIFK
Nearest Stachelhaus code(s):: Hty DGEAVGCIFK (100% 8Å match); ohHty DGEAVGCIFK (94% 8Å match); dohHty DGEAVGCIFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4978..5381): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAQTIVAIHK
Nearest Stachelhaus code(s):: Thr DAQTIVAIHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6085..6484): X

nrpys: 3S-oh-4S-mePro

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DNTMITAMSK
Nearest Stachelhaus code(s):: 3S-oh-4S-mePro DNTMITAMSK (100% 8Å match); 3OH-Pro or 3S-oh-4S-mePro DNTMITAMSK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

aniI: X

AMP-binding (30..459): X

nrpys: Lin

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: AYVYIGGVFK
Nearest Stachelhaus code(s):: Lin AYVYIGGVFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor