LOCUS       BGC0001388              6188 bp    DNA     linear   BCT 15-MAR-2016
DEFINITION  Lactobacillus gasseri strain EV1461 gassericin E gene cluster,
            partial sequence.
ACCESSION   BGC0001388
VERSION     BGC0001388
KEYWORDS    .
SOURCE      Lactobacillus gasseri
  ORGANISM  Lactobacillus gasseri
            Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
            Lactobacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 15:27:20
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6188
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /db_xref="taxon:1596"
                     /host="Homo sapiens"
                     /isolation_source="vagina from healthy woman"
                     /mol_type="genomic DNA"
                     /organism="Lactobacillus gasseri"
                     /strain="EV1461"
     misc_feature    <1..>6188
                     /note="gassericin E gene cluster"
     subregion       1..6188
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6188
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            141..293
                     /gene="gaeP"
     CDS             141..293
                     /codon_start=1
                     /gene="gaeP"
                     /note="autoinducer peptide for bacteriocin production"
                     /product="GaeP"
                     /protein_id="ALX37941.1"
                     /transl_table=11
                     /translation="MKEIRKENIVKTTTLSEEEMIEVVGGTRVNGFVNGNFSKGIFYWT
                     RNIWK"
     gene            296..1603
                     /gene="gaeK"
     CDS             296..1603
                     /EC_number="2.7.13.3"
                     /codon_start=1
                     /gene="gaeK"
                     /note="two-component histidine kinase"
                     /product="GaeK"
                     /protein_id="ALX37943.1"
                     /transl_table=11
                     /translation="MLLVYSFLMLISGITDLWIFFRVSQIAASKKNILILGGVLFLLPI
                     LCGGVVELATFLEILFFIFYFRKSKEKNWAIGAILIVGVVDSIIDILSSILMELLHIED
                     IIYLNLLVQISIILLAVFIIEIFYKYLHSYLMKENHNVFIGLLIYLYVSTTLTMIFYGQ
                     TQKNASLSMFFTIFVLIQIVFAMATYVELINIQKHLLKKSQQDNLIKEQKQLQDYAQYL
                     EESEDELRAFRHDYRNMFNSLKISAQEGNTKEVIQKLDEYTKANLNAKAFEKYRDVNHI
                     KIKSLKSIIIAKLTEMYREHISYNFECSSEITKLPKNINEIDLVRIVGISCDNAIEESK
                     VLIEQNQEAHIEIMAYSNERGEFEYEIQNKRGDSEISIKQIRQNGYSTKKSHSGLGLAN
                     INSIKNKYENMTISYEVPEGYFDFYLVIEPEEESEE"
     gene            1600..2397
                     /gene="gaeR"
     CDS             1600..2397
                     /EC_number="3.1.1.61"
                     /codon_start=1
                     /gene="gaeR"
                     /note="two-component response regulator"
                     /product="GaeR"
                     /protein_id="ALX37942.1"
                     /transl_table=11
                     /translation="MKYSVIVCDDDEVLAKNLAKNIKYAVSNFTDDNPVYENIEINLEL
                     VANTFEQVVSYVVANDIQNAIYFLDIELSQNSKAKNGVDLAEFIKKQDPNAQIIFVTAY
                     DKYAPLTYRRRIGAIDYINKALDSREMMQRLEETLTGAVQSINNLMKSGRKELIYKIGR
                     RVNKVEDTTIYYLENSTSQHKVNLITETGVAEFRSNISKINEENDFLVKVSQSCVVNPN
                     NINSIDFSKKTIKFPNGDEVVFARSCRKTVEDLLNKYPEINVK"
     gene            2410..4569
                     /gene="gaeT"
     CDS             2410..4569
                     /codon_start=1
                     /gene="gaeT"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 335.3; E-value:
                     1.2e-101)"
                     /gene_kind="biosynthetic"
                     /note="bacteriocin ABC-transporter"
                     /product="GaeT"
                     /protein_id="ALX37944.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.4e-46, bitscore:
                     146.1, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1072.3,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLFKYKSVYVPQVDEMDCGVACLAMILKQYHSRVSLAHLRHEART
                     NLEGTTALGLVKTAQKFNFKTEAVKADMSLFDENSIQYPFIAHVLKQGEILHYYVVLKN
                     AKNYLVIADPDPSVGVVKMPKDKFAQEWTGVALFMVPNEDFEPIKEKKNNLWSLFPYMF
                     KQKRLMINIILAALLMTIISICSSYFVQGIIDTYIPDGTYQTLSILAVGLLIAYVFNSI
                     FSYGQNFLLNILGQRLSIDLNLRYIRHIFELPMEFFVTRRTGEITSRFSDASRIIDALA
                     STVISLFLDLSIVIVMGLVLAAQNMTLFGITLLALPIYAVVILGFTKKFEKLNNEQMES
                     NAVLSSSVIEDIQGIETIKALNSENTRYRRIDSQFVDYLKKSFKYSKTESLQTALKTFI
                     QLSLNVIVLWVGAKIVMQGQLSIGQLMTFNALLAYFIDPLQSIINLQPRLQSASVAQNR
                     LNEVYQVKSEFNQKATIEDRKLLEGNIEYKNVDYSYGYGTDVLKDINLKISQGEKLTIV
                     GMSGSGKSTMVKLLVDFFSPSKGQVTLNGHATSEIDKHTLRSYVNYVPQTPYIFSGTVK
                     ENLLLGCRPDITEEDVIKACQIAEIDQEIANLPLQFETKLDENAKILSGGQKQRLTIAR
                     ALLSPAKVFIFDEVTSGLDTITEKKVIDNLMKLKDKTIIFIAHRLAIAERADKVVVIDH
                     GQIVEEGSHSELMSKHGFYYDLVKG"
     gene            4580..5173
                     /gene="gaeC"
     CDS             4580..5173
                     /codon_start=1
                     /gene="gaeC"
                     /note="accessory protein for ABC-transporter"
                     /product="GaeC"
                     /protein_id="ALX37945.1"
                     /transl_table=11
                     /translation="MDAKEYESTEFYSYKFKNFSTMIIIPAAIFVLLLFIGSFFAVRQS
                     TVSSVGVVEPTVVINQKNVSYDEGQVVTKHGQKWVAHVDQDSGISLMPVMKAKGKVRIV
                     TYVLTNKISSIKKGQKLNFSVPTVDGLNSRLTGKVKEIGVYPINVNKQSMYEVISIAIV
                     SDINVKYGMQGNATIVTGRSTYFNYFLDKVLNKR"
     gene            5241..5342
                     /gene="gaeZ"
     CDS             5241..5342
                     /codon_start=1
                     /gene="gaeZ"
                     /note="unknown function"
                     /product="GaeZ"
                     /protein_id="ALX37946.1"
                     /transl_table=11
                     /translation="MLDKNIDLQRAIFHIKQDINLYSVVYGFKLPET"
     gene            5475..5702
                     /gene="gaeA"
     CDS             5475..5702
                     /codon_start=1
                     /gene="gaeA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /note="bacteriocin; Experimental evidence; One peptide of
                     the putative two-component bacteriocin (GasE+GaeX);
                     Gassericin E"
                     /product="GasE"
                     /protein_id="ALX37947.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.9e-19,
                     bitscore: 58.6, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNFNTLSFETLANIVGGRNNLAANIGGVGGATVAGWALGNAVCG
                     PACGFVGAHYVPIAWAGVTAATGGFGKIRK"
     gene            5712..5909
                     /gene="gaeX"
     CDS             5712..5909
                     /codon_start=1
                     /gene="gaeX"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /note="one peptide of the putative two-component
                     bacteriocin (GasE+GaeX); Putative"
                     /product="putative GaeX"
                     /protein_id="ALX37948.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 4.3e-14,
                     bitscore: 42.0, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALKTLEKHELRNVMGGNKWGNAVIGAATGATRGVSWCRGFGPWG
                     MTACGLGGAAIGGYLGYKSN"
     gene            5925..>6188
                     /gene="gaeI"
     CDS             5925..>6188
                     /codon_start=1
                     /gene="gaeI"
                     /note="putative immunity protein to Gassericin E"
                     /product="GaeI"
                     /protein_id="ALX37949.1"
                     /transl_table=11
                     /translation="MLFFVTIINVILFLGNLYQTGFNYFNFNQLQALLLTIFLIVTVIV
                     IAINKKLERGYDLSFFALYLYWPIYVFEVKLYNSTNYKDGIEF"
ORIGIN
        1 aattgataac ttaagaataa taataaaata ccgaattagt cgtaaaagtg actgattcgg
       61 tatttttggt attttcgagg gatgaaagat ggtaatattt aagacataaa aataatattt
      121 attatttaag gagaagaata atgaaagaaa ttagaaaaga aaatattgtt aaaactacta
      181 ctttgagtga agaagaaatg attgaagttg ttggtggtac tagagtaaat ggttttgtta
      241 atggaaattt tagtaaaggt atattttact ggacgcgcaa catttggaaa taaaaatgtt
      301 gttagtttat agctttttaa tgcttatttc tggaataact gatttatgga tattttttag
      361 agtttctcaa attgcagcta gtaaaaaaaa tattttgatt ctcggtggag tgttattctt
      421 acttccaatt ttatgtggag gagttgttga actagccact tttttggaaa ttttattttt
      481 catattttat tttagaaaga gtaaagaaaa aaattgggct attggggcaa ttttaattgt
      541 aggagttgta gattccataa tagatatact aagtagtatc ttaatggagc ttttacatat
      601 tgaggatata atttatctta atttattagt ccaaattagt attatacttc ttgcagtttt
      661 tataattgag atcttctata agtatcttca ctcatattta atgaaagaaa atcacaatgt
      721 gtttatagga ctgttgatct atctatatgt ttccactact ttaacaatga ttttttatgg
      781 acaaacccag aaaaatgcat cgttaagtat gttttttaca atttttgtat taattcaaat
      841 agtttttgct atggcgactt acgtggaact aataaatatt caaaagcatc ttttaaaaaa
      901 atcacaacaa gataatttaa taaaagagca aaaacaatta caagactatg cgcaatatct
      961 tgaagagagc gaagatgagt tacgtgcttt tcggcatgat tatcgaaata tgtttaactc
     1021 attaaaaatc agtgcacagg aaggaaatac taaagaagta atccaaaagc ttgatgaata
     1081 tactaaggcc aatttaaatg caaaagcatt tgaaaaatat agagatgtta atcatataaa
     1141 aataaaatct ctcaaaagta tcattattgc taagttaaca gaaatgtata gagaacatat
     1201 ttcatataat tttgaatgta gcagtgaaat tacgaaattg cctaaaaata ttaatgaaat
     1261 agatttagtc agaatcgtcg gtataagttg tgacaatgcg attgaagaaa gcaaagtatt
     1321 aatagagcaa aatcaagaag ctcatattga aattatggct tattcaaacg aacgtggtga
     1381 atttgaatat gaaatacaaa ataaacgagg tgattctgaa atttcaatta aacaaatacg
     1441 acaaaacggt tattcaacaa agaaaagtca ctctggcttg ggcttagcca atatcaatag
     1501 tattaaaaat aaatatgaaa atatgactat ttcttatgaa gtgcctgaag gatactttga
     1561 tttctatctc gtgattgaac cagaggagga aagtgaagaa tgaaatattc cgtaatcgtt
     1621 tgtgatgatg acgaagtatt agcaaaaaat ttagcgaaaa atattaaata tgcagttagt
     1681 aactttaccg atgataatcc agtctacgaa aatattgaaa tcaatttaga gctagttgct
     1741 aatacatttg agcaagttgt aagttatgta gttgctaatg atattcaaaa tgcaatttac
     1801 tttttagata ttgaattaag tcaaaattca aaagctaaaa acggagtaga cttagcagaa
     1861 tttattaaaa agcaagatcc aaatgcacaa ataatttttg ttactgctta tgacaaatat
     1921 gctcctttaa cttatcgtag aagaattggt gctattgatt atattaataa ggccttagat
     1981 tcaagagaaa tgatgcagcg attagaagaa accttgactg gcgccgttca aagtatcaat
     2041 aatcttatga aatcaggaag aaaagaactt atctataaaa ttggacggag agttaataaa
     2101 gttgaagaca cgactattta ttatcttgaa aatagtactt cacaacacaa agttaatttg
     2161 ataactgaaa ctggtgtagc tgaattcaga agtaatatct ccaaaattaa cgaagaaaat
     2221 gattttttag ttaaggtatc tcaatcttgt gtagtaaatc caaataatat caacagcatc
     2281 gacttttcaa aaaagacaat aaagtttcct aatggagatg aagtagtgtt cgctcgtagt
     2341 tgcagaaaaa ctgttgagga tctattaaat aaatatccag aaattaatgt taagtaaaag
     2401 gtaagactta tgttattcaa atataaatcc gtatacgtgc cacaagtgga tgagatggat
     2461 tgcggcgttg cttgtctagc aatgatctta aagcaatatc attctcgcgt atctttagca
     2521 catttacgtc atgaagctcg tactaatctt gaaggcacaa ctgctttagg actagtgaag
     2581 acagcacaaa aatttaattt caaaacagaa gccgtaaaag cagatatgtc tttatttgat
     2641 gagaatagta ttcaatatcc ttttattgcc cacgtcctaa aacaaggaga aatacttcat
     2701 tattacgtag ttcttaaaaa tgctaagaat tatttagtaa ttgcagatcc tgatccatca
     2761 gttggtgtag taaagatgcc aaaagacaaa tttgctcaag aatggactgg tgttgcactc
     2821 tttatggttc ctaacgaaga ctttgaacca atcaaagaaa agaagaataa tttatggtct
     2881 ctctttccat atatgtttaa acaaaagcgg ctgatgatta acatcatttt agccgcttta
     2941 ttaatgacca taattagtat ctgtagttca tattttgttc aaggaataat tgatacttac
     3001 attccagatg gaacctatca gactctttcg atcttagctg ttggactatt aattgcttat
     3061 gtctttaatt caattttttc ttatggacag aattttttat taaatatttt aggtcaaaga
     3121 ttaagtattg atctaaatct gcgatacatc aggcatattt ttgaattacc aatggaattc
     3181 tttgtaacaa gaagaacggg tgaaattact tcgcgttttt ctgatgcaag tagaattatt
     3241 gacgctttgg caagtacagt tatttcgctc ttcttagacc tctcaattgt gattgtgatg
     3301 ggattagtct tagcagcaca aaatatgaca ttatttggga ttacactgtt agctttgcct
     3361 atttatgcag ttgtaattct aggttttact aaaaagtttg aaaaactaaa taacgaacaa
     3421 atggaaagta atgctgtttt gagttcttca gttattgaag atattcaagg aattgaaact
     3481 attaaagctt taaatagtga gaatacaaga tatagaagaa tcgatagtca gtttgtagat
     3541 tacttaaaga agtcatttaa atatagtaag actgagagtt tacagacagc actcaagaca
     3601 tttattcaat tatctcttaa tgtaattgtt ctttgggttg gtgcaaagat tgtaatgcaa
     3661 ggacaattga gcattggtca gttaatgacg ttcaatgcat tgctggcata tttcattgat
     3721 cccttgcaaa gtattattaa tttacaaccg cgacttcaat cagctagtgt tgctcaaaat
     3781 cgattaaatg aggtctatca agtaaaaagt gaattcaatc agaaggctac tattgaagat
     3841 cgtaagctcc tagaaggaaa tattgaatat aagaatgtgg attatagcta tggttatggc
     3901 acagatgtct taaaagatat taatctcaaa attagccagg gcgaaaaatt aacgattgtt
     3961 ggaatgagtg gttctggaaa atctacgatg gttaagttgt tagtagattt cttttcgcca
     4021 agtaaaggtc aggttacatt aaatggacat gcaacaagtg aaattgataa gcatacgttg
     4081 cggtcatatg taaattatgt acctcaaact ccatatatct tttctggaac agttaaagag
     4141 aatttgttgt taggttgtag gccagatatc actgaagaag atgtaataaa ggcttgtcaa
     4201 attgctgaga ttgaccaaga aatagctaat ctacctttgc aatttgaaac aaaattagat
     4261 gaaaatgcca aaattttatc tggtggacaa aaacaaaggt taactattgc ccgggcatta
     4321 ctatctcctg caaaggtatt tatttttgat gaagtaacaa gtggcttaga tacaattact
     4381 gagaagaaag tgattgataa tttgatgaag ctaaaggata agacaattat ctttattgcg
     4441 catcgactag caattgcaga acgggcagac aaggtagttg taattgatca tggtcaaata
     4501 gttgaagaag gcagccacag cgaattgatg agtaagcatg gtttttacta tgacttagtg
     4561 aagggatagg tgagtacaga tggatgcaaa agagtatgaa agtacagaat tctattccta
     4621 taagttcaag aatttctcga caatgattat tattccagcc gctatttttg ttctactttt
     4681 atttattgga tctttttttg ctgttagaca gagtacagtc tcttcggttg gcgttgttga
     4741 accaacggtt gtaattaatc agaaaaatgt tagctatgat gagggacaag tcgttacaaa
     4801 acatggtcaa aaatgggtag ctcatgttga tcaagatagt gggattagtt taatgcctgt
     4861 tatgaaagct aaaggaaaag tgaggattgt tacctatgtg ctcactaata aaatctcgtc
     4921 tattaagaag gggcagaagt taaacttttc tgtacctaca gtagacggct tgaatagtag
     4981 attaactggt aaggtaaagg agattggtgt ttatccaata aatgtgaaca agcaaagtat
     5041 gtatgaagtt atttcgattg ccatagttag tgatataaat gttaagtacg gaatgcaagg
     5101 taatgccaca atagtgacag gtcgtagcac atattttaac tattttttag ataaggtatt
     5161 gaataaaaga taaatttgat agaataaaaa attaaaattt tattaatcta aagataaaaa
     5221 tataagtgaa taaaaaagag atgttagata agaatattga tttgcaaaga gcaatatttc
     5281 atataaagca agatattaat ttgtattctg tagtgtatgg atttaaattg cctgaaactt
     5341 aattttggga acgaatttgg ataaaatata ctgttttggt tagtaagtaa gcaatattaa
     5401 tccatataaa aaataaattg ttttttatag taggctaaat atataaagat tattaattat
     5461 aggaggtctt tattatgaaa aattttaata cattatcatt tgaaacattg gctaacatag
     5521 ttggtgggag aaataatttg gctgctaata taggtggagt aggtggagcg acagtcgctg
     5581 gatgggctct tggaaatgca gtttgcggtc ctgcttgtgg ctttgttgga gcacactatg
     5641 ttccaatagc atgggctggc gtaacggcag ctactggtgg attcggaaag ataagaaagt
     5701 agggaattaa tatggcttta aaaacattag aaaaacatga attaagaaat gtaatgggtg
     5761 gaaacaagtg ggggaatgct gtaataggag ctgctacggg agctactcgc ggagtaagtt
     5821 ggtgcagagg attcggacca tggggaatga ctgcctgtgg cttaggaggt gctgcaattg
     5881 gaggatatct gggatataag agtaattaat ttatatagag atttatgtta ttctttgtaa
     5941 ctattattaa tgtaatactt tttttaggta acttgtatca aacgggcttt aattatttta
     6001 actttaatca attgcaagca cttttactaa ctatttttct tattgttact gtaatagtta
     6061 tcgctattaa taaaaaatta gaaagaggtt atgatctttc tttttttgca ctttatttgt
     6121 attggccaat ttatgttttt gaagttaaat tatataattc aacaaattat aaagatggaa
     6181 tagaattt
//
