LOCUS       BGC0001391             10393 bp    DNA     linear   BCT 10-JUL-2016
DEFINITION  Bacillus thuringiensis serovar rongseni thusin biosynthesis gene
            cluster, complete sequence.
ACCESSION   BGC0001391
VERSION     BGC0001391
KEYWORDS    .
SOURCE      Bacillus thuringiensis serovar rongseni
  ORGANISM  Bacillus thuringiensis serovar rongseni
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:27:50
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 10393
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..216
                     /gene="thsA1"
     CDS             1..216
                     /codon_start=1
                     /gene="thsA1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_ha"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="ThsA1"
                     /protein_id="ANP43731.1"
                     /sec_met_domain="mature_ha (E-value: 2.8e-10, bitscore:
                     29.0, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDVIKNRKKIEEKLELPEILLEEVEEHSAMGGINTWNTTATSTS
                     IIISETFGNKGKVCTYTVECVNNCRG"
     CDS_motif       1..99
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence:
                     MLDVIKNRKKIEEKLELPEILLEEVEEHSAMGG"
                     /locus_tag="thsA1_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       100..216
                     /prepeptide="core"
                     /locus_tag="thsA1_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-3.40"
                     /molecular_weight="3929.4"
                     /monoisotopic_mass="3927.0"
                     /core_sequence="INTWNTTATSTSIIISETFGNKGKVCTYTVECVNNCRG"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MLDVIKNRKKIEEKLELPEILLEEVEEHSAMGG"
                     /leader_location="[0:99](+)"
                     /alternative_weights="3947.4"
                     /alternative_weights="3965.4"
                     /alternative_weights="3983.4"
                     /alternative_weights="4001.5"
                     /alternative_weights="4019.5"
                     /alternative_weights="4037.5"
                     /alternative_weights="4055.5"
                     /alternative_weights="4073.5"
     source          1..10393
                     /db_xref="taxon:180865"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis serovar rongseni"
                     /serovar="rongseni"
                     /strain="BGSC 4BT1"
     misc_feature    1..10393
                     /note="thusin biosynthesis gene cluster"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2418:8779]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2419..8779
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..10393
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001391"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            285..2435
                     /gene="thsT"
     CDS             285..2435
                     /codon_start=1
                     /gene="thsT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 229.8; E-value: 1e-69)"
                     /gene_kind="transport"
                     /product="ThsT"
                     /protein_id="ANP43732.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.5e-41, bitscore:
                     129.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKIPIVLQLQQTECGLCCSNMILNYYGSRETIFNLRNEFEVGRD
                     GLSLSQLKQLFLKKGFIPKIYKADISGLKKLQLPAIIFWENKHFVVLEEISDKKIKIID
                     PAEGKRKLTTEEFLNSFTHLVLEVVPSNDFKPQFAEEVKPWEGIKQLVINKLPLTIFII
                     LTTIIVLLLNLIIPYLTQKIIDGALLGSVFNDISRIILSLILISIFHLILSITRGSLII
                     KLNIFLSEKLLGNTFRHLLRLPFKFFDLRSPGDLLYRLNSLIGVRDLISSSLIPGIVDL
                     GMMFVILIYLSSKSYTITLISLGLLFFNFLILSLSRPKVATAVDNELVEQSKSQAILTE
                     ILFSSLFIKIAGLENKFFSKWELSYKKSLEKFKYRSQLQNIINSLSGNFQLIAPVIILV
                     TGIILYQNHVLTLGEIVAIQTITTMLFNQSSSVFSAYTQLILTSSYLRRVSDITETKIE
                     SSNNLIKDFEVNGNIELEDVYFSYTNMSNDIIKNINLSINSGDKIAIVGKSGSGKSTLA
                     KIIVGLYKHTKGNIYIDGLNINSIDQSVLHKQIGIVPQDIMLFNKSIFENITMGNEKFT
                     LEDVQEAAKLAMIDKEIENMPMQYNTVISDMGLNLSGGQRQRISLARALINKPRILILD
                     EATSSLDQENEFNIMEYFKKIKCTVIIIAHRLSTIIDADKIYVIDEGTIIETGTHHELM
                     NQGGFYYHLYNKKGVSDYAYNQ"
     gene            2419..5442
                     /gene="thsM1"
     CDS             2419..5442
                     /codon_start=1
                     /gene="thsM1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     810; E-value: 5.2e-245)"
                     /gene_kind="biosynthetic"
                     /product="ThsM1"
                     /protein_id="ANP43733.1"
                     /sec_met_domain="DUF4135 (E-value: 5.7e-92, bitscore:
                     297.8, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 1.9e-63, bitscore:
                     203.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPIINKKNELNNCMQLYPELQSTEELEDLIYRVSFTTIDELYKKS
                     ENENKFSFKFHNLNTENVIDIGISSDLPFGSFCKSIIIDPLNRFFKDLKKIKIINNYTY
                     FTKNVADQLISRIWELSYKTLIHELKILKSENLLIGETEETRFAYYHDVLLEDKEYIEY
                     ILSENKELSNILSLIINRSLKNTLTILHRTEDEWNKLLTQFCINKNAKVTLIDVGMGDT
                     HQNGQTVCTLHFSDQSRIMYKPRNLEVDQAFQEFLKFLNTKKVGKFNTVNFFSSGSDGW
                     MEFISPKECTEGKQINEFYKKTGQYLAILYILNANDIHYENIIAHGENPMLIDLESLFV
                     PKPNHYFEEFDSSFQKAQEFLHTSVNSIALLPTEITRSVDGTDLYADLGGVSFHKKQIS
                     PYKSVILNNKNLESMDFSLKNLELEPKHNNPVLNGITLEAYDYIQDIRNGFKNMYEWAI
                     ENREELVSKIQQLFIGKRIRFINKSTMFYSKLLDISTYPIFSKNSFYKKLLFHRVGLDG
                     FKSGNPFLKHEYNDLLNMDVPYFSAITDKKKVYNSNGDDIGDILNETPLERCLTKIDNL
                     SLTDLSKQLKLIEMSFLNKRKGHEDCTKISFVQGKSNHPKHTINYHSYLKTAKNIGDHL
                     INEMITADENAYWIAPSLEGEQENIWSPGLVSLDLYSGNAGISIFLAQLYSCTGEVKYK
                     DAAYKAIEPVRKFIIELEENSQLPFPLGVFSGTSGYFYAIYKTGIFLEDTKLENLFFDN
                     IDKLKSFVIKDKTFDVIGGVTGTIHMLLNIAANCHVTKNKNHLIHLSIELEKHLRQHPV
                     FKDQGKLYAGFSHGIAGVMSCYALLYKNTKNRIYKSNVLELRDKLNTFYSPVQKNWYNS
                     NQNSDISNGWCHGAPGILLSFLICMECGIEIDEMYVKIAVNSTKDKALGNNITYCHGDL
                     GTLSILERYANQLNDSVLSSQVELTFERLHQRYLKEIVSKNTIRHSYSFSLMIGITGAG
                     LALINKLNKIRTNILWID"
     gene            5473..5661
                     /gene="thsA2"
     CDS             5473..5661
                     /codon_start=1
                     /gene="thsA2"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="ThsA2"
                     /protein_id="ANP43734.1"
                     /transl_table=11
                     /translation="MLKEEKLEKITGLIPESELEEHLSGESSGAGTPAITTAISAIIAA
                     TAQSPCPTSACSKSCNK"
     CDS_motif       5473..5547
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MLKEEKLEKITGLIPESELEEHLSG"
                     /locus_tag="thsA2_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       5548..5661
                     /prepeptide="core"
                     /locus_tag="thsA2_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-4.80"
                     /molecular_weight="3280.7"
                     /monoisotopic_mass="3278.7"
                     /core_sequence="ESSGAGTPAITTAISAIIAATAQSPCPTSACSKSCNK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MLKEEKLEKITGLIPESELEEHLSG"
                     /leader_location="[5472:5547](+)"
                     /alternative_weights="3298.7"
                     /alternative_weights="3316.7"
                     /alternative_weights="3334.7"
                     /alternative_weights="3352.7"
                     /alternative_weights="3370.8"
                     /alternative_weights="3388.8"
                     /alternative_weights="3406.8"
                     /alternative_weights="3424.8"
                     /alternative_weights="3442.8"
     gene            5741..5926
                     /gene="thsA2'"
     CDS             5741..5926
                     /codon_start=1
                     /gene="thsA2_"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="ThsA2'"
                     /protein_id="ANP43735.1"
                     /transl_table=11
                     /translation="MKEQKLEKITGLIPESELEEHLSGESSGAGTPAITTAISAIIGAT
                     AQSPCPTSACSKSCNK"
     CDS_motif       5741..5872
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence:
                     MKEQKLEKITGLIPESELEEHLSGESSGAGTPAITTAISAIIGA"
                     /locus_tag="thsA2__lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       5873..5926
                     /prepeptide="core"
                     /locus_tag="thsA2__lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-4.30"
                     /molecular_weight="1604.8"
                     /monoisotopic_mass="1603.7"
                     /core_sequence="TAQSPCPTSACSKSCNK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKEQKLEKITGLIPESELEEHLSGESSGAGTPAITTAISAI
                     IGA"
                     /leader_location="[5740:5872](+)"
                     /alternative_weights="1622.8"
                     /alternative_weights="1640.8"
                     /alternative_weights="1658.9"
     gene            5996..8779
                     /gene="thsM2"
     CDS             5996..8779
                     /codon_start=1
                     /gene="thsM2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     787.4; E-value: 3.4e-238)"
                     /gene_kind="biosynthetic"
                     /product="ThsM2"
                     /protein_id="ANP43736.1"
                     /sec_met_domain="DUF4135 (E-value: 1.8e-88, bitscore:
                     286.3, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 2.6e-61, bitscore:
                     196.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYKKTIEKNIKEYETIFGDFYRPILLDLEDIIENLLNTYREHINE
                     KEIILNDIINSLLYELNRISFRTLLYEFHLTKDENEVNDQDHHFKNYIKLLKDSNYITS
                     ILDKYEHLKQLIKQRIQFKIELIEKIFEHFSNDKSKLKQHIDNEIHAIHGIEFSHGDSH
                     NQGKSVTILQTNSGKLVYKPHNLYTDQWFYEILNVLKEDINIDMPEIPLFTVDDHGWQG
                     FIYHKPCTKTEELSNYYYRMGIYLSVFYLLKTDDIHYENIIASGEYPFILDLETIVANK
                     KIHCDEEKESLMYTFLESITNSVLGTMILPVNYKFSPFDVDISALSTKEAESNIWLSYV
                     IEDAGTDNIRLIKQPSPLNKENNKVMLHNKVANPFDFLNSIESGFSHGYDSILKNKNKI
                     TEICHSNLVNKINVRHIPRATSVYAKFLDASTHPTYLRKTETVKTLFNKFRRGTSFTEI
                     KQAESEISALLSHDIPYFTQPLTSKSLYCNEMSVIEDYFQDRPIDLVLNRLKNMGIKDK
                     EKQLYYIKMSMVTDGSNNLTNIHGNQILGFKNTEIDSILKEVGNELIDTAIIYKDTCTW
                     MIHEVTDNEKVYIGPLNYCLYDGAGVLLFLLELASYSNNKKYLQYAEMGLKGLDTDIEV
                     SKIDSHSVFHGIGSYLYLYARFYNTTQKTEYYHKLLDGVKKLDITYKKDLNIDFIGGLS
                     GMIVFLTNCYRAYGDEIFYKKANEFAELMISVYKEKSEFLNGLAHGHAGISLSLASLYE
                     INNDYEISNEAYKIIKQENLHFNSNDNNWTDLREFINTESVYWCHGAPGISLARMKTIQ
                     YLGISDLLDKDLKCSIRKTLQDGFSDSHDSSLCHGVFGNLDILIEINKYKKCETLSHFI
                     DATFSSYIDNLTKFGYQSGIKNSLPINNFMLGKFGIAYTLLRYKNNKIPCILSLDI"
     gene            8791..9672
                     /gene="thsF"
     CDS             8791..9672
                     /codon_start=1
                     /gene="thsF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 182; E-value:
                     2.4e-55)"
                     /gene_kind="transport"
                     /product="ThsF"
                     /protein_id="ANP43737.1"
                     /transl_table=11
                     /translation="MKGRYLLSIEITNLSKNFNKRERVLDSINMNIKNGEIVGLVGPNG
                     AGKTTLMKIISGLIVQYNGEIKFSQEEYTQIGSLIEKPKYFPNKSGYYNLKYFSQLYSD
                     KDNNIEEIIHSLQMENYLSKKVKDYSLGMKQRLGIALALLNNPNYLILDEPTNGMDPNG
                     IKSILKYIKGLAKKKNIGVLLSSHILDDVADISDRVYLINKGQIINEYSSSITPEKTIK
                     FIFNQEIIIEATNILSYFGLVEKTSNTISLTTDKDLKILLEKLGEKNIYPQDIDVKKDT
                     LEEFYFKNIGAE"
     gene            9674..10393
                     /gene="thsE"
     CDS             9674..10393
                     /codon_start=1
                     /gene="thsE"
                     /product="ThsE"
                     /protein_id="ANP43738.1"
                     /transl_table=11
                     /translation="MNFSSYIRLELFRNISTVYLYLAIIASFIFGGGIVYSIYSLNGPF
                     HANNVSGLYSTIAVLALAIFCVKVVSIDLYYGTIQLIFTNKQNRHLFLYTRLIIITVIS
                     ILFGIGCISLLWINDVLNHQGFNVQIVCNTLFHYFLFGIFYTLLFLLISIFYKKPMNLL
                     IIAFFTIVLTPSIIGAILNIPALPSILKEGIMVLPIYTLPIKIADLSLSITDIFVIISS
                     NVCLFFWIFYTLPKTDY"
ORIGIN
        1 gtgcttgatg ttattaaaaa cagaaaaaag atcgaagaaa agttagaatt gcctgaaatt
       61 ttattggaag aagttgaaga acactccgct atggggggga taaacacatg gaatactaca
      121 gctacttcga cttcaatcat tatcagtgaa acatttggta ataaagggaa agtttgtact
      181 tacacagttg aatgtgtgaa taattgtaga ggttaattaa aaaatgagag taataacttt
      241 tattactctc attatctact aagaaaggtt tatattaata taaaatgaaa aaaattccaa
      301 tagttttaca actccaacaa actgaatgtg gattatgctg tagtaacatg atattaaatt
      361 attatggcag tcgagaaact attttcaatt taaggaatga atttgaggtt ggtagagatg
      421 gattaagcct tagtcaatta aaacaattat ttttgaagaa aggttttatt ccaaaaattt
      481 ataaagctga tattagtgga cttaaaaaac ttcaacttcc cgcaataatt ttttgggaaa
      541 acaaacattt tgttgttcta gaagagattt cggacaaaaa gattaaaatt attgatcccg
      601 ctgaagggaa acggaaactt actaccgagg agtttttaaa tagttttaca catttggtcc
      661 tagaggtagt cccaagtaat gactttaagc cacaattcgc tgaagaagta aaaccttggg
      721 agggaataaa acaattagtc attaacaaac tgcctttaac gatattcatt attttaacaa
      781 caataatcgt attactactc aatttaatta ttccatacct cacacaaaaa attatagatg
      841 gtgctctact cggatcagta tttaatgata tttctaggat tattttaagt ctcatactta
      901 tatctatatt tcacttaatc ctatcaatta caagaggatc gcttattatt aaactgaata
      961 tttttttaag tgaaaagctt ttggggaaca cattccgcca tttattacga ttgccattta
     1021 aatttttcga tttaagatct ccaggggatt tattgtatag actgaatagt cttattggag
     1081 taagggattt aatttctagt agtttaatac caggtatcgt tgatttaggt atgatgtttg
     1141 ttattctaat ttatttgtct tcgaaatcat atacaattac acttatcagt ttagggttat
     1201 tattttttaa ctttttaatt ttaagtttaa gtagacctaa agttgctacc gcagtagata
     1261 atgaactggt tgagcaaagt aaaagtcagg ctatcttgac tgaaattctg ttttctagtt
     1321 tatttattaa aattgcaggg ttagaaaata aatttttttc taagtgggaa cttagttata
     1381 agaaaagttt agagaaattt aaataccgtt cccagttgca aaatataata aactctttat
     1441 ctggtaactt tcaacttata gctcctgtca taattttagt aacaggtata attttatacc
     1501 aaaatcatgt attgacacta ggtgaaattg tagcaattca aactattaca acaatgctat
     1561 ttaatcaatc tagttcggta ttttctgctt atacacaact tattttgact tcatcttatt
     1621 taagaagagt gagtgatatt acagaaacta aaatagagtc ttctaataat ctcattaaag
     1681 actttgaagt taatggaaat atagagttgg aagatgttta tttttcatat acgaatatga
     1741 gcaatgacat tatcaaaaac attaatttat ctattaatag tggtgataaa attgctattg
     1801 ttggaaaatc tggttctgga aaaagtacat tagcaaaaat aatagttgga ctatataaac
     1861 ataccaaagg aaatatctac atagatggtc tgaatatcaa ttccattgat caaagcgtat
     1921 tacataaaca aataggtatt gttccacaag atattatgtt attcaataaa agcatctttg
     1981 aaaatataac aatgggaaat gaaaaattca cattagaaga tgtgcaagag gcagcaaaac
     2041 ttgcaatgat tgataaagaa attgaaaaca tgcctatgca gtataacacg gtaataagcg
     2101 atatgggatt gaacttatca ggtggccaac gtcagcgtat ttctttagct agggctctta
     2161 ttaataaacc acgaatatta attttagatg aggcaacaag ctcattggat caagagaacg
     2221 agtttaatat tatggagtat tttaaaaaga ttaagtgtac agtaattatt atagctcatc
     2281 gactttcaac tattatagac gccgacaaaa tatatgttat tgatgagggg actatcatag
     2341 agactggaac tcatcatgaa ttaatgaatc aaggtggctt ctattatcac ttatataata
     2401 aaaaaggagt aagtgattat gcctataatc aataaaaaaa atgaattgaa caattgtatg
     2461 caattatatc ctgagttaca aagtacagaa gaactggaag atttaattta tagagtttca
     2521 tttactacca tagatgagct ctataagaaa agtgaaaatg aaaataaatt tagctttaaa
     2581 ttccataatc tgaatactga aaatgtaatt gatataggta tttcatcaga tttacctttt
     2641 ggttcatttt gtaaatctat tattattgat cccctaaatc gctttttcaa agatttaaag
     2701 aaaatcaaga ttataaataa ttatacctat tttacaaaaa atgttgctga tcagttaatt
     2761 tcaagaatat gggaattatc atataaaact ttaattcatg aattaaaaat attaaaaagt
     2821 gaaaacttat taattggaga aacagaagaa acacggttcg catattatca tgatgtttta
     2881 ttagaagata aagaatatat agaatatata ttgagtgaaa ataaagaatt atcaaatata
     2941 ttgagtctta tcataaacag aagtttaaaa aatactttaa ctattttaca tagaacagaa
     3001 gatgaatgga ataaactatt aacccaattt tgtatcaata agaatgcgaa agttaccctt
     3061 attgatgtag gaatgggaga tactcatcaa aatggacaaa ccgtttgtac acttcatttt
     3121 tcagatcagt cacgaattat gtataaacca agaaacttag aggtggatca agcattccaa
     3181 gaatttttaa agtttttaaa tacaaagaaa gtaggtaaat ttaatactgt taactttttt
     3241 tctagtggtt cagatggatg gatggaattt atttcaccaa aagaatgtac tgaaggaaag
     3301 caaattaatg aattttacaa aaagactgga cagtacctag ctatactata tattttaaac
     3361 gcaaatgata ttcactatga aaatattatt gcacacgggg aaaatccaat gctaatcgat
     3421 ttagaatcct tatttgttcc aaaacctaat cattatttcg aagaatttga cagcagtttt
     3481 caaaaagctc aagaattttt acatacatct gtaaatagta ttgcactttt accaacagaa
     3541 ataactagat ccgttgatgg cactgattta tatgcagatt taggtggtgt gagttttcat
     3601 aagaaacaaa tatctcccta taaatcagta atattaaaca ataaaaattt agaaagtatg
     3661 gatttttctt taaaaaattt agaattagaa ccaaaacata ataatcctgt tttaaatggt
     3721 atcacattag aagcgtatga ttatattcaa gacattcgaa atgggtttaa aaatatgtat
     3781 gagtgggcca ttgaaaatag agaagaatta gtaagtaaaa ttcaacaatt atttattggt
     3841 aaaagaattc gattcatcaa taaaagcact atgttttact cgaaattatt agacatatca
     3901 acatacccta tttttagtaa aaattctttt tataagaaat tattattcca cagagttggt
     3961 ttagacggat ttaaatcggg aaaccctttc ttaaaacatg aatacaatga tttgttaaat
     4021 atggatgttc cttatttttc tgcaataact gacaaaaaaa aggtctacaa ttctaatggt
     4081 gatgatattg gggacatact aaatgaaact cctttagaac gttgtttaac aaagatagat
     4141 aatttatctt taacagatct ttccaaacaa ctaaaattaa ttgaaatgtc atttctaaat
     4201 aaacgtaaag gacacgaaga ttgtactaaa atctcatttg tacaaggaaa atctaatcat
     4261 ccaaaacaca cgataaatta tcattcctat ctgaaaaccg ctaagaatat cggtgatcat
     4321 ttaattaatg aaatgattac cgcggatgaa aatgcatatt ggattgctcc ctcactggaa
     4381 ggagaacaag aaaacatctg gtctccaggg ctcgtaagtt tagatttata tagtggaaat
     4441 gctgggattt cgatattttt agcacaacta tactcatgta cgggtgaagt aaaatataaa
     4501 gatgccgctt ataaggcaat tgaacctgta aggaaattta taatagagct agaagaaaat
     4561 agccagctac ctttccctct aggagtattc tctggaactt ccggttattt ttatgctatt
     4621 tataagactg gtatttttct agaagatact aaacttgaaa atttattttt tgataatatc
     4681 gataaattga aaagttttgt tatcaaggat aaaacttttg atgttatagg tggcgtaaca
     4741 ggtacaattc acatgttatt aaatattgca gctaattgtc atgttactaa gaataaaaat
     4801 cacttaattc acctttctat cgaactagaa aaacatttac gtcaacatcc cgtatttaaa
     4861 gatcaaggaa aactttacgc tggtttctct catggtatag caggagttat gtcgtgctat
     4921 gcattattat acaaaaatac caaaaaccga atatacaaaa gtaatgtttt agaattaaga
     4981 gataaattaa atactttcta ctctccagtt caaaaaaact ggtataacag caatcagaat
     5041 agtgatatca gtaatggttg gtgtcatgga gctcctggta tactactctc cttcttaatt
     5101 tgtatggagt gtggaataga aatagatgaa atgtatgtaa aaattgctgt aaatagcaca
     5161 aaagataaag ctttaggtaa taacatcact tattgtcatg gtgatttggg tactttatct
     5221 atacttgaga gatacgcgaa tcaactaaac gattcggttc tctcttcaca agtcgaacta
     5281 acatttgaac gtttacacca acgatactta aaagaaatag ttagtaaaaa tacaattaga
     5341 cattcatatt catttagctt aatgattggt ataactggtg ctggtctagc tttaattaat
     5401 aaattgaata aaataagaac taatattcta tggattgatt aatcctttct tacttgaaag
     5461 ggggtgaaac aaatgttaaa agaggaaaaa ttagaaaaaa tcacaggact tatcccagaa
     5521 tcagagcttg aagaacatct ttcaggagaa agtagcggtg cagggactcc agctattact
     5581 actgcaatta gtgcaattat tgcagcaact gcacaatctc catgtccaac atctgcatgt
     5641 tcaaaatcat gcaataaata atttcaatac ataatataag aataataatt tatgtattaa
     5701 ttaattctat tttaacttgg aaaggaaatg atgaatcgtg atgaaagaac aaaaattaga
     5761 aaaaatcaca ggacttatcc cagaatcaga acttgaagaa catctctcag gagaaagtag
     5821 cggtgcagga actccagcta ttactactgc aattagtgca attattggag caaccgcgca
     5881 atctccatgt ccaacatctg catgttcaaa atcatgcaat aaataggtag taagtagcaa
     5941 aatagattgt ttcgatgctt agatcgaaac aatctattta ttaggaggga aaactttgta
     6001 caaaaaaact atagagaaaa acataaaaga atatgaaact atttttgggg atttttatag
     6061 accaattcta ctagatctcg aagatataat tgaaaattta cttaatacat acagagaaca
     6121 tataaatgaa aaggaaataa ttcttaatga tatcataaat tccttattat atgagttaaa
     6181 tagaatctca ttcagaactt tattatatga attccattta actaaagatg aaaatgaagt
     6241 aaatgaccaa gaccaccact ttaaaaatta cataaaatta ttaaaggatt cgaattatat
     6301 cacatcaata ttagataaat atgagcactt aaaacaatta attaaacaaa gaattcaatt
     6361 taaaatagaa ttaattgaaa agatttttga acatttttcg aatgacaaat ctaaattaaa
     6421 gcagcatata gacaatgaaa ttcatgccat acatggaatt gaatttagcc atggtgattc
     6481 tcataatcaa ggtaaatcgg taactattct acaaactaat agtgggaagt tagtatataa
     6541 acctcataat ttatatactg atcaatggtt ttatgaaatt ttaaatgttt taaaggaaga
     6601 tattaatatt gatatgccag aaatcccact atttactgta gatgatcatg gatggcaagg
     6661 cttcatatat cacaaaccat gtacaaaaac agaagaactt tcaaattatt attatagaat
     6721 gggaatatac ttatccgttt tttatcttct taaaacagat gatattcatt atgaaaatat
     6781 tatagcctcg ggggaatatc cttttatact tgacctggaa actatagtcg ctaacaaaaa
     6841 aattcattgt gatgaagaaa aagaatcttt aatgtatact ttcttagaat ctataactaa
     6901 ctctgtctta ggaacaatga ttttaccggt aaattacaaa ttttcaccat ttgacgtgga
     6961 tatttctgca ttatctacta aagaagctga atcaaacatt tggttatctt atgtaataga
     7021 agatgcaggg acagataata ttcgattaat aaagcagcca tctccattaa ataaagaaaa
     7081 taataaggtc atgttacata ataaagttgc taatcctttt gattttctca acagtattga
     7141 gagtggattt tcacatggat atgatagtat tttaaaaaac aaaaataaaa ttacagaaat
     7201 atgccactca aacttagtaa ataaaatcaa tgtaagacat atcccacggg ctacgagtgt
     7261 atatgcaaag tttttagatg cttctacgca tcccacttac cttcgcaaaa cagaaacagt
     7321 aaaaacgcta tttaataaat tcagaagagg cacttccttt acagaaataa aacaagctga
     7381 atcagaaata tcagctttac ttagccatga tatcccttat tttactcaac cattaacaag
     7441 taaaagctta tactgcaatg aaatgagtgt aatagaagat tattttcaag acagacctat
     7501 tgatttagtc cttaatagat tgaaaaatat gggtatcaaa gacaaagaaa agcaattata
     7561 ctacataaaa atgtcaatgg tcactgatgg aagcaataac cttacaaata ttcatggaaa
     7621 tcaaattcta ggctttaaaa atacagaaat tgatagcatt ttaaaagaag ttggaaatga
     7681 attgattgat acagctatta tctataaaga tacttgtaca tggatgatac atgaagtaac
     7741 agataacgaa aaagtttata taggccctct taattattgt ctatatgatg gtgcgggggt
     7801 acttcttttc ttattagaat tagcttcata tagtaataat aaaaaatatt tacaatatgc
     7861 tgaaatggga cttaaaggat tggatactga tatagaggta agtaaaatag attcacattc
     7921 agtatttcat ggaattggct cctatcttta tctatatgca agattttata atactacaca
     7981 aaaaactgag tattaccata aattactgga tggtgtaaaa aaattagata taacatataa
     8041 aaaagattta aacatagatt ttattggtgg attatctgga atgatagtat ttctaacaaa
     8101 ttgttatagg gcatatggtg atgaaatttt ctataaaaaa gcaaacgagt ttgctgaatt
     8161 aatgatttct gtttataaag agaaatcaga gttcttaaat ggccttgccc atgggcatgc
     8221 tggaatttca ttatctctgg catcactata tgaaataaat aatgattacg aaattagtaa
     8281 tgaagcatat aaaatcatta aacaagaaaa tcttcatttt aattctaatg ataataactg
     8341 gacagactta agagaattta ttaatactga atcagtatac tggtgtcatg gcgctccagg
     8401 catttcatta gctagaatga aaacaataca atacctaggt ataagtgatt tactagataa
     8461 agatttaaaa tgtagcattc gtaaaaccct tcaagatgga ttttctgatt ctcatgatag
     8521 tagcttatgt catggagtat ttggtaactt agatatttta atagaaatca acaaatataa
     8581 aaaatgcgaa actttaagcc attttattga tgctacattt tctagttata tagataatct
     8641 tactaagttt ggatatcaat ctgggataaa aaactcttta cctattaaca actttatgct
     8701 tgggaaattt ggaattgctt atactttact tagatataag aataataaaa tcccatgtat
     8761 attatcttta gacatttaag taattattat atgaagggaa gatatctttt gagcattgaa
     8821 attactaatc tatcaaaaaa ctttaataaa agagaaagag ttttagactc tataaacatg
     8881 aatattaaaa atggtgaaat agttggatta gttggtccta acggggcggg aaaaactaca
     8941 ttaatgaaaa ttatctcagg actcattgta cagtataatg gtgaaatcaa attctctcaa
     9001 gaagaatata ctcaaatagg tagcttaatt gaaaaaccaa aatattttcc taataaaagt
     9061 ggttactata atctgaaata ttttagtcag ttatatagtg ataaagataa taatattgaa
     9121 gaaattatcc attcccttca aatggaaaat tatttatcta aaaaagtaaa agattattct
     9181 ttagggatga agcaaaggct gggaattgct ttagcattgt taaacaatcc aaattattta
     9241 atattagatg aaccaactaa tggaatggat cctaatggga ttaaaagtat cttaaaatat
     9301 ataaaagggt tagcaaagaa aaaaaatata ggggttctct tatctagtca tattttagat
     9361 gatgtagcgg atattagcga tcgggtttac ctaattaaca aagggcagat tataaatgaa
     9421 tattctagct caattactcc tgaaaagact attaaattta tttttaatca agaaataatc
     9481 attgaggcaa caaatatttt atcctatttt ggattagtag agaagacgtc aaatacaatt
     9541 tcattgacta ccgataaaga tttaaaaata ttattagaaa agttagggga aaaaaatatt
     9601 taccctcaag atatagatgt aaaaaaagat actttagaag agttttactt taagaatata
     9661 ggagctgagt aatatgaact tttcaagcta tattaggttg gaattattta gaaatataag
     9721 tactgtatac ctttatctag ctattattgc atcatttatt tttggaggtg gaatagttta
     9781 ttccatttat tccttaaatg gtccatttca tgcaaataat gtttcgggtc tctactcaac
     9841 tatcgctgtt cttgcattag ctatattttg tgtgaaagtt gtttcaatag atttatacta
     9901 tgggactata cagttaattt ttacaaataa acagaataga catttgttcc tttatacaag
     9961 gctaatcatt ataacagtta tctcaattct atttggcatc ggttgtatat ccttgctatg
    10021 gataaatgat gtattaaacc atcaaggatt caatgtacaa attgtttgca acacattatt
    10081 tcattatttt ttatttggaa ttttttacac ccttttattt ttattaattt ctattttcta
    10141 taaaaagcct atgaatcttc ttataattgc attctttaca attgtactca cgccaagtat
    10201 cattggagca attttaaata ttcctgcttt gccttctata ttaaaagagg gaattatggt
    10261 gttgcccata tataccttgc ctataaaaat agctgattta tcgttaagta taactgatat
    10321 atttgtaatt atcagttcga acgtttgttt gtttttctgg atattctata ctcttcctaa
    10381 aactgattat taa
//
