BGC0001394 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001394 (Myxococcus stipitatus DSM 14675)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	56,241	phenalamide A2	NRPS:Type I+PKS	100%

BGC0001394 - Region 1 - NRPS,T1PKS

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Location: 1 - 56,241 nt. (total: 56,241 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 56,242
Label		BGC0001394

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001394	1.28	NRP, Polyketide	phenalamide A2	Myxococcus stipitatus DSM 14675
BGC0001737	0.77	NRP, Polyketide	phenalamide	Corallococcus coralloides
BGC0001187	0.54	NRP, Polyketide	xenolozoyenone	Glarea lozoyensis
BGC0001244	0.50	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001273	0.50	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001874	0.49	NRP, Polyketide	cyclopiazonic acid	Aspergillus flavus
BGC0001276	0.49	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275	0.48	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001758	0.48	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002324	0.47	NRP, Polyketide	myxochelin B, myxochelin N, myxochelin O, myxochelin P, myxochelin Q, myxochelin A	Archangium violaceum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001394	1.00	NRP, Polyketide	phenalamide A2	Myxococcus stipitatus DSM 14675
BGC0001737	0.85	NRP, Polyketide	phenalamide	Corallococcus coralloides
BGC0001535	0.68	Other	branched-chain fatty acids	Streptomyces filamentosus
BGC0001244	0.64	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0001273	0.64	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001022	0.64	NRP, Polyketide	myxalamid, myxalamid A, myxalamid C, myxalamid D	Stigmatella aurantiaca
BGC0001275	0.64	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276	0.64	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001068	0.62	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0002229	0.61	Polyketide	6-MSA, aculin A	Aspergillus aculeatus ATCC 16872

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 56241:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal) + (Me-ccmal) + (Me-ccmal) + (Me-ccmal) + (pk - pk - ccmal) + (Me-ccmal) + (Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MYSTI_04320

Search NORINE for peptide: strict or relaxed

AMP-binding (538..928): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

MYSTI_04321

PKS_AT (567..865): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 175.2
Ethylmalonyl-CoA: 154.5
Methoxymalonyl-CoA: 137.0
Malonyl-CoA: 106.4
2-Methylbutyryl-CoA: 90.5
Benzoyl-CoA: 83.8
Isobutyryl-CoA: 83.7
Propionyl-CoA: 81.1
trans-1,2-CPDA: 76.9
Acetyl-CoA: 71.4
CHC-CoA: 70.2
3-Methylbutyryl-CoA: 39.4
fatty_acid: 36.0
inactive: 33.0

MYSTI_04322

PKS_AT (561..859): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; Methylmalonyl-CoA: 83.3%; Ethylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 144.8
Malonyl-CoA: 130.4
Ethylmalonyl-CoA: 128.6
Methoxymalonyl-CoA: 114.2
Acetyl-CoA: 90.4
Isobutyryl-CoA: 85.7
2-Methylbutyryl-CoA: 79.2
Benzoyl-CoA: 76.3
Propionyl-CoA: 65.1
trans-1,2-CPDA: 59.1
CHC-CoA: 43.0
inactive: 32.7
fatty_acid: 25.9
3-Methylbutyryl-CoA: 22.7

PKS_AT (2383..2679): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 141.4
Malonyl-CoA: 130.7
Methoxymalonyl-CoA: 128.1
Isobutyryl-CoA: 119.4
Ethylmalonyl-CoA: 110.6
2-Methylbutyryl-CoA: 77.6
Propionyl-CoA: 76.3
Acetyl-CoA: 73.8
Benzoyl-CoA: 72.0
trans-1,2-CPDA: 62.8
3-Methylbutyryl-CoA: 61.0
inactive: 57.0
CHC-CoA: 49.1
fatty_acid: 42.0

PKS_AT (3908..4201): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.2
inactive: 164.4
Methoxymalonyl-CoA: 90.1
Methylmalonyl-CoA: 84.1
Propionyl-CoA: 71.0
Ethylmalonyl-CoA: 61.8
2-Methylbutyryl-CoA: 58.6
Isobutyryl-CoA: 44.5
CHC-CoA: 39.3
Benzoyl-CoA: 35.1
trans-1,2-CPDA: 33.0
Acetyl-CoA: 29.1
fatty_acid: 14.8
3-Methylbutyryl-CoA: 12.0

MYSTI_04323

PKS_AT (567..864): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.2
Ethylmalonyl-CoA: 162.5
Methoxymalonyl-CoA: 137.7
Malonyl-CoA: 103.6
Isobutyryl-CoA: 95.1
trans-1,2-CPDA: 90.1
Benzoyl-CoA: 87.4
Acetyl-CoA: 78.8
2-Methylbutyryl-CoA: 75.8
CHC-CoA: 63.1
Propionyl-CoA: 57.6
3-Methylbutyryl-CoA: 49.2
inactive: 31.5
fatty_acid: 19.0

MYSTI_04324

PKS_AT (560..856): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 176.9
Ethylmalonyl-CoA: 150.2
Methoxymalonyl-CoA: 130.2
Malonyl-CoA: 108.8
Isobutyryl-CoA: 96.7
Benzoyl-CoA: 90.0
trans-1,2-CPDA: 80.3
2-Methylbutyryl-CoA: 73.4
Acetyl-CoA: 67.3
Propionyl-CoA: 59.4
CHC-CoA: 54.9
3-Methylbutyryl-CoA: 41.8
inactive: 21.8
fatty_acid: 18.4

MYSTI_04325

PKS_AT (558..854): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%; Propionyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 185.3
Ethylmalonyl-CoA: 171.5
Methoxymalonyl-CoA: 151.0
Isobutyryl-CoA: 116.1
Malonyl-CoA: 93.7
Benzoyl-CoA: 90.7
trans-1,2-CPDA: 90.5
2-Methylbutyryl-CoA: 86.4
Propionyl-CoA: 85.3
CHC-CoA: 78.9
fatty_acid: 70.7
Acetyl-CoA: 58.1
3-Methylbutyryl-CoA: 41.4
inactive: 23.9

MYSTI_04326

PKS_AT (540..836): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 197.5
Ethylmalonyl-CoA: 177.0
Methoxymalonyl-CoA: 154.0
Isobutyryl-CoA: 135.2
Benzoyl-CoA: 123.5
Malonyl-CoA: 113.9
2-Methylbutyryl-CoA: 100.2
Acetyl-CoA: 88.6
Propionyl-CoA: 87.4
trans-1,2-CPDA: 86.6
CHC-CoA: 75.7
3-Methylbutyryl-CoA: 49.0
fatty_acid: 34.6
inactive: 26.8