BGC0001394 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001394 (Myxococcus stipitatus DSM 14675)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	89,882	High	phenalamide A2	NRPS:Type I+PKS

BGC0001394 - Region 1 - NRPS,T1PKS

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Location: 1 - 89,882 nt. (total: 89,882 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 89,883
Label		BGC0001394

Tailoring functions

Transferases: acylase

MYSTI_04321: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

MYSTI_04323: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Ligases: ligase synthase

MYSTI_04320: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

MYSTI_04322: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

MYSTI_04324: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

MYSTI_04325: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

MYSTI_04326: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

MYSTI_04328: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001394.5	1.27	NRPS, PKS	phenalamide A2	Myxococcus stipitatus DSM 14675
BGC0001244.3	0.81	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.81	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.79	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.79	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001737.3	0.76	NRPS, PKS	phenalamide	Corallococcus coralloides
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001268.4	0.67	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002192.3	0.66	PKS	FR901512	fungal sp. No.14919
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001394.5	1.00	NRPS, PKS	phenalamide A2	Myxococcus stipitatus DSM 14675
BGC0001737.3	0.85	NRPS, PKS	phenalamide	Corallococcus coralloides
BGC0001244.3	0.77	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001268.4	0.67	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002192.3	0.67	PKS	FR901512	fungal sp. No.14919
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 89882:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal) + (Me-ccmal) + (Me-ccmal) + (Me-ccmal) + (pk - pk - ccmal) + (Me-ccmal) + (Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

MYSTI_04320: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (538..928): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

MYSTI_04321: mmal

PKS_AT (567..865): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 175.2
Ethylmalonyl-CoA: 154.5
Methoxymalonyl-CoA: 137.0
Malonyl-CoA: 106.4
2-Methylbutyryl-CoA: 90.5
Benzoyl-CoA: 83.8
Isobutyryl-CoA: 83.7
Propionyl-CoA: 81.1
trans-1,2-CPDA: 76.9
Acetyl-CoA: 71.4
CHC-CoA: 70.2
3-Methylbutyryl-CoA: 39.4
fatty_acid: 36.0
inactive: 33.0

MYSTI_04322: pk - pk - mal

PKS_AT (561..859): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; Methylmalonyl-CoA: 83.3%; Ethylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 144.8
Malonyl-CoA: 130.4
Ethylmalonyl-CoA: 128.6
Methoxymalonyl-CoA: 114.2
Acetyl-CoA: 90.4
Isobutyryl-CoA: 85.7
2-Methylbutyryl-CoA: 79.2
Benzoyl-CoA: 76.3
Propionyl-CoA: 65.1
trans-1,2-CPDA: 59.1
CHC-CoA: 43.0
inactive: 32.7
fatty_acid: 25.9
3-Methylbutyryl-CoA: 22.7

PKS_AT (2383..2679): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 141.4
Malonyl-CoA: 130.7
Methoxymalonyl-CoA: 128.1
Isobutyryl-CoA: 119.4
Ethylmalonyl-CoA: 110.6
2-Methylbutyryl-CoA: 77.6
Propionyl-CoA: 76.3
Acetyl-CoA: 73.8
Benzoyl-CoA: 72.0
trans-1,2-CPDA: 62.8
3-Methylbutyryl-CoA: 61.0
inactive: 57.0
CHC-CoA: 49.1
fatty_acid: 42.0

PKS_AT (3908..4201): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 211.2
inactive: 164.4
Methoxymalonyl-CoA: 90.1
Methylmalonyl-CoA: 84.1
Propionyl-CoA: 71.0
Ethylmalonyl-CoA: 61.8
2-Methylbutyryl-CoA: 58.6
Isobutyryl-CoA: 44.5
CHC-CoA: 39.3
Benzoyl-CoA: 35.1
trans-1,2-CPDA: 33.0
Acetyl-CoA: 29.1
fatty_acid: 14.8
3-Methylbutyryl-CoA: 12.0

MYSTI_04323: mmal

PKS_AT (567..864): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.2
Ethylmalonyl-CoA: 162.5
Methoxymalonyl-CoA: 137.7
Malonyl-CoA: 103.6
Isobutyryl-CoA: 95.1
trans-1,2-CPDA: 90.1
Benzoyl-CoA: 87.4
Acetyl-CoA: 78.8
2-Methylbutyryl-CoA: 75.8
CHC-CoA: 63.1
Propionyl-CoA: 57.6
3-Methylbutyryl-CoA: 49.2
inactive: 31.5
fatty_acid: 19.0

MYSTI_04324: mmal

PKS_AT (560..856): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 176.9
Ethylmalonyl-CoA: 150.2
Methoxymalonyl-CoA: 130.2
Malonyl-CoA: 108.8
Isobutyryl-CoA: 96.7
Benzoyl-CoA: 90.0
trans-1,2-CPDA: 80.3
2-Methylbutyryl-CoA: 73.4
Acetyl-CoA: 67.3
Propionyl-CoA: 59.4
CHC-CoA: 54.9
3-Methylbutyryl-CoA: 41.8
inactive: 21.8
fatty_acid: 18.4

MYSTI_04325: mmal

PKS_AT (558..854): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%; Propionyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 185.3
Ethylmalonyl-CoA: 171.5
Methoxymalonyl-CoA: 151.0
Isobutyryl-CoA: 116.1
Malonyl-CoA: 93.7
Benzoyl-CoA: 90.7
trans-1,2-CPDA: 90.5
2-Methylbutyryl-CoA: 86.4
Propionyl-CoA: 85.3
CHC-CoA: 78.9
fatty_acid: 70.7
Acetyl-CoA: 58.1
3-Methylbutyryl-CoA: 41.4
inactive: 23.9

MYSTI_04326: mmal

PKS_AT (540..836): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 197.5
Ethylmalonyl-CoA: 177.0
Methoxymalonyl-CoA: 154.0
Isobutyryl-CoA: 135.2
Benzoyl-CoA: 123.5
Malonyl-CoA: 113.9
2-Methylbutyryl-CoA: 100.2
Acetyl-CoA: 88.6
Propionyl-CoA: 87.4
trans-1,2-CPDA: 86.6
CHC-CoA: 75.7
3-Methylbutyryl-CoA: 49.0
fatty_acid: 34.6
inactive: 26.8

Link to PARAS substrate predictor