BGC0001409 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001409 (Streptomyces minoensis)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T2PKS,T1PKS,PKS-like	1	59,215	dutomycin	PKS	100%

BGC0001409 - Region 1 - PKS-like,T1PKS,T2PKS

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Location: 1 - 59,215 nt. (total: 59,215 nt) Show pHMM detection rules used

Region on contig edge.

T2PKS: (t2ks and t2clf)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
PKS-like: (t2pks2 or ksIII)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 59,216
Label		BGC0001409

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001409	2.50	Polyketide	dutomycin	Streptomyces minoensis
BGC0000231	1.07	Polyketide	griseusin A, griseusin B	Streptomyces griseus
BGC0001283	0.96	Polyketide	arsono-polyketide	Streptomyces lividans 1326
BGC0001124	0.89	Polyketide	pyranonigrin E	Aspergillus niger ATCC 1015
BGC0002729	0.88	Polyketide	sordarial	Neurospora crassa OR74A
BGC0001749	0.88	Polyketide	epipyriculol	Pyricularia oryzae 70-15
BGC0002227	0.87	NRP	AKML B, AKML D, AKML A, AKML C	Aspergillus luchuensis IFO 4308
BGC0001514	0.87	Other	aristeromycin	Streptomyces citricolor
BGC0001913	0.87	Polyketide	phomoidride	fungal sp. ATCC 74256
BGC0002266	0.86	Terpene, Polyketide	calidoustene A, calidoustene B, calidoustene C	Aspergillus calidoustus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001409	1.00	Polyketide	dutomycin	Streptomyces minoensis
BGC0002284	0.70	Other	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0001283	0.67	Polyketide	arsono-polyketide	Streptomyces lividans 1326
BGC0000231	0.66	Polyketide	griseusin A, griseusin B	Streptomyces griseus
BGC0001061	0.65	Polyketide, Saccharide	polyketomycin	Streptomyces diastatochromogenes
BGC0002435	0.65	Other	coformycin, aristeromycin	Micromonospora haikouensis
BGC0001928	0.65	Other	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0000131	0.65	Polyketide	pyrrolomycin	Streptomyces sp. UC 11065
BGC0001749	0.64	Polyketide	epipyriculol	Pyricularia oryzae 70-15
BGC0002496	0.64	NRP	serratiochelin A, serratiochelin C, serratiochelin B	Serratia plymuthica

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Type II PKS

Predicted core structure(s)

For candidate cluster 1, location 0 - 59215:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 16245 - 59215:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 22843 - 59215:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

dutL

AMP-binding (13..392): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: PMNYFGGPMK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

dutG

PKS_AT (552..847): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 165.7
Ethylmalonyl-CoA: 157.7
Methoxymalonyl-CoA: 138.3
trans-1,2-CPDA: 102.4
Malonyl-CoA: 94.3
2-Methylbutyryl-CoA: 86.3
Isobutyryl-CoA: 80.6
Propionyl-CoA: 76.3
Benzoyl-CoA: 69.5
CHC-CoA: 64.8
Acetyl-CoA: 61.8
3-Methylbutyryl-CoA: 44.5
inactive: 34.9
fatty_acid: 27.5

Type II PKS gene cluster prediction details

Cluster section:: 0 to 59215
Starter unit(s):: malonamyl-CoA (Score: 425.0; E-value: 4.55e-148)
propionyl-CoA (Score: 124.0; E-value: 1.6e-33)
Malonyl elongations:: 8|9 (Score: 635.0; E-value: 9.8e-194)
Product class(es):: anthracycline
aureolic acid
tetracycline
Product molecular weight(s):: malonamyl-CoA_8: 800 Da
malonamyl-CoA_9: 842 Da
propionyl-CoA_8: 771 Da
propionyl-CoA_9: 813 Da

dutA: KS (Score: 758.6; E-value: 3.6e-231)
dutB: CLF 8|9 (Score: 635.0; E-value: 9.8e-194)
dutC: ACP (Score: 109.9; E-value: 1.1e-34)
dutD: CYC C7-C12 (Score: 321.4; E-value: 1.1e-98)
dutE: CYC C1-C18|C2-C19 (Score: 228.5; E-value: 5.3e-71)
dutF: CYC C5-C14/C3-C16 (Score: 445.7; E-value: 1.1e-136)
dutG: AT (Score: 164.9; E-value: 5.7e-51); AT BAB72049.1_Aklavinone_propionyl-CoA (Score: 124.0; E-value: 1.6e-33)
dutGT1: GT (Score: 356.5; E-value: 5.5e-109)
dutGT2: GT (Score: 345.7; E-value: 1.1e-105)
dutI: KR (Score: 137.5; E-value: 1.5e-42)
dutJ: KR (Score: 196.9; E-value: 1.1e-60)
dutL: LIG (Score: 502.7; E-value: 2.2e-153); LIG ADE34493.1_SF2575_malonamyl-CoA (Score: 425.0; E-value: 4.55e-148)
dutMT1: MET C6|C8 (Score: 546.9; E-value: 3.4e-167)
dutMT2: MET C11O (Score: 472.9; E-value: 1.2e-144)
dutO1: OXY (Score: 186.7; E-value: 1.8e-57)
dutO2: OXY (Score: 411.4; E-value: 1.3e-125)
dutO4: OXY (Score: 473.7; E-value: 1.6e-144)