BGC0001413 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001413 (Cystobacter sp. Cbv34)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS	1	52,081	cystobactamid 919-1/cystobactamid 919-2/cystobactamid 919-3	NRPS:Type I	100%

BGC0001413 - Region 1 - NRPS

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Location: 1 - 52,081 nt. (total: 52,081 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 52,082
Label		BGC0001413

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001413	0.97	NRP	cystobactamid 919-1, cystobactamid 919-2, cystobactamid 919-3	Cystobacter sp. Cbv34
BGC0000426	0.38	NRP	sevadicin	Paenibacillus larvae
BGC0001050	0.37	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0002518	0.36	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001128	0.35	NRP	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833	0.35	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286	0.35	NRP	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132	0.35	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135	0.34	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002017	0.33	NRP	potensibactin	Nocardiopsis potens DSM 45234

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001413	0.99	NRP	cystobactamid 919-1, cystobactamid 919-2, cystobactamid 919-3	Cystobacter sp. Cbv34
BGC0000426	0.70	NRP	sevadicin	Paenibacillus larvae
BGC0001050	0.70	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0002518	0.69	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001128	0.68	NRP	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833	0.68	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286	0.68	NRP	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132	0.68	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135	0.67	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002017	0.66	NRP	potensibactin	Nocardiopsis potens DSM 45234

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 52081:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X - X) + (Asn) + (X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

cysG

AMP-binding (40..438): X

nrpys: ohnBza

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AIKYYSINDK
Nearest Stachelhaus code(s):: ohnBza AIKYYSINDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1100..1500): X

nrpys: 2,3-doh-4-nBza

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AVKYIANDIK
Nearest Stachelhaus code(s):: 2,3-doh-4-nBza AVKYIANDIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

cysH

Search NORINE for peptide: strict or relaxed

AMP-binding (37..436): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

cysK

AMP-binding (263..681): X

nrpys: pNH2-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AVKHIANNDK
Nearest Stachelhaus code(s):: pNH2-Bz AVKHIANNDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1337..1755): X

nrpys: pNH2-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AVKHIANNDK
Nearest Stachelhaus code(s):: pNH2-Bz AVKHIANNDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2406..2827): X

nrpys: Orn, Lys, Arg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLAYFGVIGK
Nearest Stachelhaus code(s):: Ser DVAYSGMIWK (47% 8Å match); Fo-OH-Orn DINYWGGIGK (44% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (3506..3924): X

nrpys: pNH2-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AVKHIANNDK
Nearest Stachelhaus code(s):: pNH2-Bz AVKHIANNDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

cysL

AMP-binding (538..919): X

nrpys: pNH2-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AYNFIAGSTK
Nearest Stachelhaus code(s):: pNH2-Bz AYNFIAGSTK (100% 8Å match)
Stachelhaus code match:: 100% (strong)