BGC0001413 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001413 (Cystobacter sp. Cbv34)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS	1	52,081	High	cystobactamid 919-1/cystobactamid 919-2/cystobactamid 919-3	NRPS:Type I

BGC0001413 - Region 1 - NRPS

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Location: 1 - 52,081 nt. (total: 52,081 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 52,082
Label		BGC0001413

Tailoring functions

Oxidoreductases: oxygenase

cysJ: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

Transferases: methyltransferase

cysF: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Hydrolases: esterase

cysE: thioesterase

smcogs: SMCOG1004: thioesterase

Isomerases: isomerase

cysD: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase

cysG: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

cysK: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

cysL: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001413.5	0.99	NRPS	cystobactamid 919-1, cystobactamid 919-2, cystobactamid 919-3	Cystobacter sp. Cbv34
BGC0001128.4	0.78	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.78	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.77	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.77	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.77	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.76	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.75	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001249.5	0.75	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001825.4	0.75	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001413.5	0.99	NRPS	cystobactamid 919-1, cystobactamid 919-2, cystobactamid 919-3	Cystobacter sp. Cbv34
BGC0001128.4	0.78	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.78	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.77	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.77	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.77	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.76	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.75	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001249.5	0.75	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001825.4	0.75	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 52081:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X - X) + (Asn) + (X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

cysG: X - X

AMP-binding (40..438): X

nrpys: ohnBza

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AIKYYSINDK
Nearest Stachelhaus code(s):: ohnBza AIKYYSINDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1100..1500): X

nrpys: 2,3-doh-4-nBza

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AVKYIANDIK
Nearest Stachelhaus code(s):: 2,3-doh-4-nBza AVKYIANDIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

cysH: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (37..436): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

cysK: X - X - X - X

AMP-binding (263..681): X

nrpys: pNH2-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AVKHIANNDK
Nearest Stachelhaus code(s):: pNH2-Bz AVKHIANNDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1337..1755): X

nrpys: pNH2-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AVKHIANNDK
Nearest Stachelhaus code(s):: pNH2-Bz AVKHIANNDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2406..2827): X

nrpys: Orn, Lys, Arg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLAYFGVIGK
Nearest Stachelhaus code(s):: Ser DVAYSGMIWK (47% 8Å match); Fo-OH-Orn DINYWGGIGK (44% 8Å match)
Stachelhaus code match:: 60% (weak)

AMP-binding (3506..3924): X

nrpys: pNH2-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AVKHIANNDK
Nearest Stachelhaus code(s):: pNH2-Bz AVKHIANNDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

cysL: X

AMP-binding (538..919): X

nrpys: pNH2-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AYNFIAGSTK
Nearest Stachelhaus code(s):: pNH2-Bz AYNFIAGSTK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor