BGC0001415 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001415 (Serratia marcescens subsp. marcescens Db11)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	30,288	althiomycin	NRPS:Type I+PKS	100%

BGC0001415 - Region 1 - NRPS,T1PKS

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Location: 1 - 30,288 nt. (total: 30,288 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 30,289
Label		BGC0001415

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001415	2.00	NRP, Polyketide	althiomycin	Serratia marcescens subsp. marcescens Db11
BGC0000955	0.73	NRP, Polyketide	althiomycin	Myxococcus xanthus
BGC0000426	0.64	NRP	sevadicin	Paenibacillus larvae
BGC0000375	0.64	NRP	indigoidine	Streptomyces chromofuscus
BGC0000900	0.62	Other	ferrichrome	Aspergillus oryzae
BGC0000351	0.52	NRP	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0001132	0.52	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000287	0.52	NRP	L-2-amino-4-methoxy-trans-3-butenoic acid	Pseudomonas aeruginosa PAO1
BGC0001560	0.51	NRP, Polyketide	cryptomaldamide	Moorea producens JHB
BGC0001841	0.50	NRP, Polyketide	detoxin P1, detoxin P2, detoxin P3	Amycolatopsis jejuensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001415	1.00	NRP, Polyketide	althiomycin	Serratia marcescens subsp. marcescens Db11
BGC0000955	0.69	NRP, Polyketide	althiomycin	Myxococcus xanthus
BGC0000426	0.65	NRP	sevadicin	Paenibacillus larvae
BGC0000375	0.65	NRP	indigoidine	Streptomyces chromofuscus
BGC0000900	0.64	Other	ferrichrome	Aspergillus oryzae
BGC0000351	0.60	NRP	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0001132	0.59	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000287	0.59	NRP	L-2-amino-4-methoxy-trans-3-butenoic acid	Pseudomonas aeruginosa PAO1
BGC0001560	0.59	NRP, Polyketide	cryptomaldamide	Moorea producens JHB
BGC0001841	0.59	NRP, Polyketide	detoxin P1, detoxin P2, detoxin P3	Amycolatopsis jejuensis

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 30288:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys - Gly) + (D-Gly - D-Cys - Ser - mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

SMDB11_2289

Search NORINE for peptide: strict or relaxed

AMP-binding (524..938): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLIDK
Nearest Stachelhaus code(s):: Gly DVWHFSLIDK (100% 8Å match); Ser DVWHFSLIDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2041..2441): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3535..3913): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code(s):: Ser DILQLGVIWK (100% 8Å match); Gly DILQLGVIWK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (4630..4922): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 152.7
inactive: 104.2
Methoxymalonyl-CoA: 61.4
Methylmalonyl-CoA: 56.7
Propionyl-CoA: 49.9
2-Methylbutyryl-CoA: 26.4
Ethylmalonyl-CoA: 24.2
Isobutyryl-CoA: 23.7
CHC-CoA: 16.6
Benzoyl-CoA: 12.1
trans-1,2-CPDA: 9.6
fatty_acid: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

SMDB11_2290

Search NORINE for peptide: strict or relaxed

AMP-binding (378..780): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVVWK
Nearest Stachelhaus code(s):: Cys DILQLGVVWK (100% 8Å match); Gly DILQLGVVWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1445..1848): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Gly DLYNLSLIWK (100% 8Å match); Cys DLYNLSLIWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)