BGC0001418 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001418 (Hyalangium minutum)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	40,460	myxochromide D	NRPS:Type I+PKS:Iterative type I	100%

BGC0001418 - Region 1 - NRPS,T1PKS

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Location: 1 - 40,460 nt. (total: 40,460 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 40,461
Label		BGC0001418

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001418	1.55	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001419	1.23	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001023	0.94	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001420	0.87	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001133	0.63	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002437	0.59	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0000342	0.58	NRP	enniatin	Fusarium equiseti
BGC0002135	0.57	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001417	0.55	NRP, Polyketide	myxochromide D	Cystobacterineae bacterium
BGC0002071	0.55	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001418	1.00	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001419	0.92	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001023	0.86	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001420	0.77	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001133	0.74	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.70	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	0.68	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002437	0.67	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0002518	0.67	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001432	0.66	NRP, Polyketide	myxochromide D	Stigmatella erecta

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 40460:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (NMe-Thr - D-Ala) + (Leu - Pro - Ala - Gln)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mchA

PKS_AT (559..855): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; CE-Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 108.1
Isobutyryl-CoA: 97.1
Malonyl-CoA: 95.6
Methoxymalonyl-CoA: 87.8
Ethylmalonyl-CoA: 70.7
Propionyl-CoA: 48.6
trans-1,2-CPDA: 44.5
Benzoyl-CoA: 43.3
CHC-CoA: 38.7
Acetyl-CoA: 37.5
2-Methylbutyryl-CoA: 31.3
inactive: 20.9
fatty_acid: 20.6
3-Methylbutyryl-CoA: 11.1

mchB

Search NORINE for peptide: strict or relaxed

AMP-binding (510..914): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2009..2399): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVWCTAAIIK
Nearest Stachelhaus code(s):: Ala DVWCTAAIIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mchC

Search NORINE for peptide: strict or relaxed

AMP-binding (469..859): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1508..1903): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GAQFIAQVVK
Nearest Stachelhaus code(s):: Pro DVQFIAQVVK (47% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2553..2943): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVWVLAAIIK
Nearest Stachelhaus code(s):: Ala DVWVLAAIIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3596..3987): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQCGLIDK
Nearest Stachelhaus code(s):: Gln DAWQCGLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)