BGC0001429 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001429 (Stigmatella aurantiaca DW4/3-1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	39,989	myxochromide S1	NRPS:Type I+PKS:Iterative type I	100%

BGC0001429 - Region 1 - NRPS,T1PKS

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Location: 1 - 39,989 nt. (total: 39,989 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 39,990
Label		BGC0001429

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001429	1.57	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca DW4/3-1
BGC0001023	1.44	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001431	1.12	NRP, Polyketide	myxochromide S	Stigmatella aurantiaca
BGC0000426	0.65	NRP	sevadicin	Paenibacillus larvae
BGC0001133	0.63	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000342	0.60	NRP	enniatin	Fusarium equiseti
BGC0002437	0.60	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0002071	0.59	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0000046	0.57	Polyketide	depudecin	Alternaria brassicicola
BGC0002135	0.56	NRP	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001429	1.00	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca DW4/3-1
BGC0001023	1.00	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001431	0.89	NRP, Polyketide	myxochromide S	Stigmatella aurantiaca
BGC0001133	0.74	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000426	0.73	NRP	sevadicin	Paenibacillus larvae
BGC0002071	0.72	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	0.67	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002437	0.67	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001833	0.67	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0000342	0.66	NRP	enniatin	Fusarium equiseti

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 39989:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (NMe-Thr - D-Leu) + (Ala - X - Ala - Gln)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mchA

PKS_AT (559..855): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; CE-Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 108.6
Isobutyryl-CoA: 100.7
Malonyl-CoA: 95.5
Methoxymalonyl-CoA: 88.9
Ethylmalonyl-CoA: 71.8
Propionyl-CoA: 49.4
trans-1,2-CPDA: 44.6
Benzoyl-CoA: 43.5
CHC-CoA: 39.1
Acetyl-CoA: 38.3
2-Methylbutyryl-CoA: 31.3
inactive: 21.3
fatty_acid: 20.7
3-Methylbutyryl-CoA: 11.1

mchB

Search NORINE for peptide: strict or relaxed

AMP-binding (510..914): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2017..2407): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWLIGAIYK
Nearest Stachelhaus code(s):: Leu DAWLIGAIYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mchC

Search NORINE for peptide: strict or relaxed

AMP-binding (467..859): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DVMFIGIVAK
Nearest Stachelhaus code(s):: Ala DVMFIGIVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1509..1904): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GAQFIAQVAK
Nearest Stachelhaus code(s):: 4Me-Pro DVQFIAHVAK (53% 8Å match); Pro DVQFIAQVVK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2557..2947): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVWVLAAIIK
Nearest Stachelhaus code(s):: Ala DVWVLAAIIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3600..3991): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQCGLIDK
Nearest Stachelhaus code(s):: Gln DAWQCGLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)