BGC0001432 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001432 (Stigmatella erecta)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	42,417	myxochromide D	NRPS:Type I+PKS:Iterative type I	100%

BGC0001432 - Region 1 - NRPS,T1PKS

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Location: 1 - 42,417 nt. (total: 42,417 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 42,418
Label		BGC0001432

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001432	2.03	NRP, Polyketide	myxochromide D	Stigmatella erecta
BGC0001023	1.18	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001418	1.02	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001419	1.01	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001417	0.99	NRP, Polyketide	myxochromide D	Cystobacterineae bacterium
BGC0001427	0.92	NRP, Polyketide	myxochromide A	Myxococcus xanthus
BGC0001426	0.92	NRP, Polyketide	myxochromide A	Myxococcus xanthus
BGC0001425	0.92	NRP, Polyketide	myxochromide A	Myxococcus xanthus DK 1622
BGC0001422	0.92	NRP, Polyketide	myxochromide A	Myxococcus fulvus HW-1
BGC0001428	0.91	NRP, Polyketide	myxochromide A	Myxococcus xanthus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001432	1.00	NRP, Polyketide	myxochromide D	Stigmatella erecta
BGC0001023	0.86	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001418	0.73	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001133	0.73	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001419	0.73	NRP, Polyketide	myxochromide D	Hyalangium minutum
BGC0001417	0.72	NRP, Polyketide	myxochromide D	Cystobacterineae bacterium
BGC0002071	0.72	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001427	0.70	NRP, Polyketide	myxochromide A	Myxococcus xanthus
BGC0001426	0.70	NRP, Polyketide	myxochromide A	Myxococcus xanthus
BGC0001425	0.70	NRP, Polyketide	myxochromide A	Myxococcus xanthus DK 1622

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 42417:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (NMe-Thr - D-Ala) + (Leu - X - Ala - Gln)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 15316 - 42417:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NMe-Thr - D-Ala) + (Leu - X - Ala - Gln)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

APZ78852.1

PKS_AT (1064..1355): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 59.1
inactive: 34.1
Methoxymalonyl-CoA: 32.1
Methylmalonyl-CoA: 26.9
Isobutyryl-CoA: 14.6
Propionyl-CoA: 11.8
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

APZ78853.1

PKS_AT (0..273): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 99.1
inactive: 44.6
Methylmalonyl-CoA: 33.5
Methoxymalonyl-CoA: 33.5
Isobutyryl-CoA: 23.7
fatty_acid: 23.5
Benzoyl-CoA: 9.5
Propionyl-CoA: 9.4
trans-1,2-CPDA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

mchA

PKS_AT (558..854): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; CE-Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 113.7
Malonyl-CoA: 103.8
Isobutyryl-CoA: 101.0
Methoxymalonyl-CoA: 92.4
Ethylmalonyl-CoA: 82.7
2-Methylbutyryl-CoA: 65.7
Propionyl-CoA: 59.3
trans-1,2-CPDA: 51.4
Benzoyl-CoA: 46.8
CHC-CoA: 41.5
Acetyl-CoA: 38.7
3-Methylbutyryl-CoA: 29.8
fatty_acid: 28.0
inactive: 24.0

mchB

Search NORINE for peptide: strict or relaxed

AMP-binding (510..914): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2017..2407): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWCTAAIIK
Nearest Stachelhaus code(s):: Ala DVWCTAAIIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mchC

Search NORINE for peptide: strict or relaxed

AMP-binding (468..859): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGNVVK
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1508..1903): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: GAQFIAQVAK
Nearest Stachelhaus code(s):: 4Me-Pro DVQFIAHVAK (50% 8Å match); Pro DVQFIAQVVK (41% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2556..2946): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVWVLAAIIK
Nearest Stachelhaus code(s):: Ala DVWVLAAIIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3599..3990): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQCGLIDK
Nearest Stachelhaus code(s):: Gln DAWQCGLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

APZ78858.1

PKS_AT (624..920): pk

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 91.7%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 66.7%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 199.8
Benzoyl-CoA: 126.9
Methylmalonyl-CoA: 113.8
Methoxymalonyl-CoA: 96.8
Ethylmalonyl-CoA: 81.2
Malonyl-CoA: 80.7
CHC-CoA: 50.1
Acetyl-CoA: 48.5
trans-1,2-CPDA: 47.3
3-Methylbutyryl-CoA: 46.4
2-Methylbutyryl-CoA: 38.4
Propionyl-CoA: 35.2
inactive: 32.9
fatty_acid: 17.5

PKS_AT (1058..1354): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 189.7
Ethylmalonyl-CoA: 174.0
Methoxymalonyl-CoA: 144.6
Isobutyryl-CoA: 121.8
Malonyl-CoA: 112.3
Benzoyl-CoA: 109.1
2-Methylbutyryl-CoA: 95.0
trans-1,2-CPDA: 81.0
Propionyl-CoA: 79.5
Acetyl-CoA: 66.9
CHC-CoA: 55.8
3-Methylbutyryl-CoA: 45.6
inactive: 31.9
fatty_acid: 30.8