BGC0001448: malacidin A biosynthetic gene cluster from uncultured bacterium
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Location: 1 - 72,298 nt. (total: 72,298 nt).
This entry is originally from NCBI GenBank KY654519.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0001448
Short description malacidin A biosynthetic gene cluster from uncultured bacterium
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
Loci
KY654519.1
via Heterologous expression
Compounds
  • malacidin A
  • malacidin B
Species uncultured bacterium [taxonomy]
References
Chemical products information
malacidin A Evidence: NMR, Mass spectrometry
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C56H88N12O20
Chemical database entries
PubCHEM
ChEBI
malacidin B Evidence: NMR, Mass spectrometry
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C57H90N12O20
Chemical database entries
PubCHEM
ChEBI
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ARU08060.1
 1 - 459 (-) hypothetical protein
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    • ARU08061.1
    		 459 - 1232 (-) hypothetical protein
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      • ARU08062.1
      		 1271 - 2197 (-) hypothetical protein
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        • ARU08063.1
        • mlcA
        		 2471 - 5602 (+) mlcA
        • Scaffold biosynthesis
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        • ARU08064.1
        • mlcB
        		 5593 - 6489 (+) mlcB
        • Regulation
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        • ARU08065.1
        • mlcC
        		 6507 - 7445 (+) mlcC
        • Regulation
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        • ARU08066.1
        • mlcD
        		 7504 - 8319 (-) mlcD
        • Transport
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        • ARU08067.1
        • mlcE
        		 8316 - 9533 (-) mlcE
        • Precursor biosynthesis
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        • ARU08068.1
        • mlcF
        		 9539 - 10012 (-) mlcF
        • Precursor biosynthesis
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        • ARU08069.1
        • mlcG
        		 10224 - 11987 (+) mlcG
        • Precursor biosynthesis
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        • ARU08070.1
        • mlcH
        		 11987 - 13420 (+) mlcH
        • Precursor biosynthesis
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        • ARU08071.1
        • mlcI
        		 13417 - 15012 (+) mlcI
        • Precursor biosynthesis
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        • ARU08072.1
        • mlcJ
        		 15023 - 15292 (+) mlcJ
        • Precursor biosynthesis
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        • ARU08073.1
        • mlcK
        		 15319 - 24513 (+) mlcK
        • Scaffold biosynthesis
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        • ARU08074.1
        • mlcL
        		 24510 - 41396 (+) mlcL
        • Scaffold biosynthesis
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        • ARU08075.1
        • mlcM
        		 41401 - 48243 (+) mlcM
        • Scaffold biosynthesis
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        • ARU08076.1
        		 48218 - 49435 (-) hypothetical protein
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          • ARU08077.1
          		 49495 - 49689 (-) hypothetical protein
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            • ARU08078.1
            		 49752 - 50345 (-) hypothetical protein
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              • ARU08079.1
              		 50606 - 51100 (+) hypothetical protein
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                • ARU08080.1
                		 51182 - 52045 (+) hypothetical protein
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                  • ARU08081.1
                  		 52042 - 52446 (-) hypothetical protein
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                    • ARU08082.1
                    • mlcN
                    		 52469 - 53380 (-) mlcN
                    • Regulation
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                    • ARU08083.1
                    • mlcO
                    		 53377 - 54312 (-) mlcO
                    • Transport
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                    • ARU08084.1
                    • mlcP
                    		 54377 - 55243 (-) mlcP
                    • Precursor biosynthesis
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                    • ARU08085.1
                    		 55242 - 55457 (+) hypothetical protein
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                      • ARU08086.1
                      • mlcQ
                      		 55515 - 56600 (-) mlcQ
                      • Precursor biosynthesis
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                      • ARU08087.1
                      • mlcR
                      		 56597 - 57397 (-) mlcR
                      • Precursor biosynthesis
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                      • ARU08088.1
                      • mlcS
                      		 57408 - 59663 (-) mlcS
                      • Precursor biosynthesis
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                      • ARU08089.1
                      • mlcT
                      		 59660 - 61381 (-) mlcT
                      • Precursor biosynthesis
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                      • ARU08090.1
                      • mlcU
                      		 62005 - 62271 (+) mlcU
                      • Regulation
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                      • ARU08091.1
                      • mlcV
                      		 62354 - 62572 (+) mlcV
                      • Scaffold biosynthesis
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                      • ARU08092.1
                      		 62712 - 63257 (+) hypothetical protein
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                        • ARU08093.1
                        • mlcW
                        		 63298 - 63984 (+) mlcW
                        • Regulation
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                        • ARU08094.1
                        		 64023 - 64817 (+) hypothetical protein
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                          • ARU08095.1
                          • mlcX
                          		 64814 - 65797 (+) mlcX
                          • Precursor biosynthesis
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                          • ARU08096.1
                          • mlcY
                          		 66442 - 69489 (+) mlcY
                          • Regulation
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                          • ARU08097.1
                          		 69828 - 70919 (-) hypothetical protein
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                            • ARU08098.1
                            		 70916 - 72298 (-) hypothetical protein
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                              Biosynthesis information

                              Biosynthetic modules

                              Name
                              2
                              Type
                              nrps-type1
                              Genes
                              mlcA
                              Substrates
                              2,3-diamino-3-methylpropanoic acid (evidence: Structure-based inference)
                              Integrated Monomers
                              Domains
                              adenylation
                              Name
                              1
                              Type
                              nrps-type1
                              Genes
                              mlcK
                              Substrates
                              3-methylaspartic acid (evidence: Structure-based inference)
                              Integrated Monomers
                              Domains
                              condensation (Starter), adenylation
                              Name
                              4
                              Type
                              nrps-type1
                              Genes
                              mlcK
                              Substrates
                              valine (evidence: Structure-based inference)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation, epimerase
                              Name
                              5
                              Type
                              nrps-type1
                              Genes
                              mlcL
                              Substrates
                              lysine (evidence: Structure-based inference)
                              Integrated Monomers
                              Domains
                              condensation, adenylation
                              Name
                              6
                              Type
                              nrps-type1
                              Genes
                              mlcL
                              Substrates
                              3-hydroxyaspartic acid (evidence: Structure-based inference)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation
                              Name
                              7
                              Type
                              nrps-type1
                              Genes
                              mlcL
                              Substrates
                              aspartic acid (evidence: Structure-based inference)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation
                              Name
                              8
                              Type
                              nrps-type1
                              Genes
                              mlcL
                              Substrates
                              glycine (evidence: Structure-based inference)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation
                              Name
                              9
                              Type
                              nrps-type1
                              Genes
                              mlcL
                              Substrates
                              3-methylaspartic acid (evidence: Structure-based inference)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation, epimerase
                              Name
                              10
                              Type
                              nrps-type1
                              Genes
                              mlcM
                              Substrates
                              valine (evidence: Structure-based inference)
                              Integrated Monomers
                              Domains
                              condensation (DCL), adenylation
                              Name
                              11
                              Type
                              nrps-type1
                              Genes
                              mlcM
                              Substrates
                              4R-methylproline (evidence: Structure-based inference)
                              Integrated Monomers
                              Domains
                              condensation (LCL), adenylation
                              Annotation changelog

                              Entry version: 4

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2022-10-07
                              Update chemical activity to schema version 2.11
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 3

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2022-09-15
                              Corrected NRP module activity
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Removed gene names of 'No gene ID'
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Fixed duplicate gene names
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2022-09-15
                              Updated NRP substrate specificities
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 2

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2019-10-16
                              Migrated from v1.4
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              2019-10-16
                              Updated compound(s) information (NPAtlas curation)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                              Entry version: 1

                              Date
                              Changes
                              Submitters
                              Reviewers
                              2018-08-06
                              Submitted
                              • (ID: IE42ACYYGHZNO4BAWZY6ERNR)
                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
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