BGC0001457 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001457 (Streptomyces caniferus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	54,671	High	jomthonic acid A/jomthonic acid B/jomthonic acid C	NRPS:Type I

BGC0001457 - Region 1 - NRPS,T1PKS

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Location: 1 - 54,671 nt. (total: 54,671 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 54,672
Label		BGC0001457

Tailoring functions

Oxidoreductases: oxygenase

ctg1_orf9: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acylase methyltransferase

ctg1_orf15: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ctg1_orf20: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Hydrolases: esterase

ctg1_orf18: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

ctg1_orf16: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ctg1_orf17: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001457.4	1.80	NRPS	jomthonic acid A, jomthonic acid B, jomthonic acid C	Streptomyces caniferus
BGC0000342.4	0.70	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.69	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001275.3	0.68	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.68	PKS	asperlactone	Aspergillus ochraceus
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002192.3	0.68	PKS	FR901512	fungal sp. No.14919
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001457.4	0.97	NRPS	jomthonic acid A, jomthonic acid B, jomthonic acid C	Streptomyces caniferus
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001275.3	0.67	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001273.3	0.67	PKS	asperlactone	Aspergillus ochraceus
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002192.3	0.67	PKS	FR901512	fungal sp. No.14919
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 54671:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_orf15: mmal - pk

PKS_AT (618..903): mmal

ATSignature: Malonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 70.8%; Isobutyryl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 144.1
Methoxymalonyl-CoA: 116.8
Malonyl-CoA: 110.1
Isobutyryl-CoA: 109.2
Ethylmalonyl-CoA: 108.7
Benzoyl-CoA: 95.7
Acetyl-CoA: 70.9
Propionyl-CoA: 68.5
2-Methylbutyryl-CoA: 68.4
CHC-CoA: 60.3
trans-1,2-CPDA: 57.6
fatty_acid: 49.7
3-Methylbutyryl-CoA: 42.8
inactive: 36.4

PKS_AT (1057..1352): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 58.3%; CE-Malonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 111.4
Malonyl-CoA: 103.1
Methoxymalonyl-CoA: 94.4
Isobutyryl-CoA: 87.2
Ethylmalonyl-CoA: 71.7
Propionyl-CoA: 54.1
CHC-CoA: 52.5
2-Methylbutyryl-CoA: 45.7
trans-1,2-CPDA: 40.3
inactive: 38.9
Acetyl-CoA: 36.7
Benzoyl-CoA: 32.0
fatty_acid: 28.7
3-Methylbutyryl-CoA: 21.7

ctg1_orf16: mal

PKS_AT (562..859): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 75.0%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 171.2
inactive: 118.7
Methoxymalonyl-CoA: 95.1
Methylmalonyl-CoA: 91.5
Propionyl-CoA: 71.2
CHC-CoA: 54.2
Ethylmalonyl-CoA: 53.0
2-Methylbutyryl-CoA: 51.7
Isobutyryl-CoA: 47.0
trans-1,2-CPDA: 28.4
3-Methylbutyryl-CoA: 27.6
Benzoyl-CoA: 22.3
fatty_acid: 17.4
Acetyl-CoA: 10.9

ctg1_orf17: X

AMP-binding (476..871): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAFVVGVIWK
Nearest Stachelhaus code(s):: Gly DIAQVGVIWK (62% 8Å match); Ph-Ser or Phe DAFTVAAIWK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor