LOCUS       BGC0001472             12862 bp    DNA     linear   BCT 05-JUN-2013
DEFINITION  Streptomyces bernensis strain UC 5144 S12 ribosomal protein (S12),
            S7 ribosomal protein (S7), elongation factor G (EF-G), and
            elongation factor Tu (EF-Tu) genes, complete cds; berninamycin gene
            cluster, complete sequence; and LmbE family protein gene, partial
            cds.
ACCESSION   BGC0001472
VERSION     BGC0001472
KEYWORDS    .
SOURCE      Streptomyces bernensis
  ORGANISM  Streptomyces bernensis
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 01:54:10
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 4867
            Orig. end    :: 17729
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     subregion       1..12862
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="RiPP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12862
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(2..703)
                     /gene="berE1"
     CDS             complement(2..703)
                     /codon_start=1
                     /gene="berE1"
                     /note="McbC-type dehydrogenase"
                     /product="BerE1"
                     /protein_id="AGN11666.1"
                     /transl_table=11
                     /translation="MRNDVTSMTAVLEGFTSRTPTSDGLAAERRPVPFADSVPVEPQPS
                     AEDLRPVHDLRGTLERRRSSLHYAPLPVRTDVILSLLRDVLRRDRDDWGLDASAGALEG
                     FVFAFRSEGAEPGLYRVTAEETCYLAGLDEIGPAENLGVQREFSTGAGIVALYASLDRA
                     DTWAGSHGYRISALRASMATYDLNLRCQALGLVGTLFGGFVPSSVHHLVHSDGATRHSL
                     LATTYARPPES"
     misc_feature    2..12862
                     /note="berninamycin gene cluster"
     gene            complement(700..2328)
                     /gene="berE2"
     CDS             complement(700..2328)
                     /codon_start=1
                     /gene="berE2"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     PF00881"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thiopeptide_F_RRE"
                     /gene_kind="biosynthetic"
                     /note="McbC-type dehydrogenase"
                     /product="BerE2"
                     /protein_id="AGN11665.1"
                     /sec_met_domain="PF00881 (E-value: 6.4e-08, bitscore: 22.2,
                     seeds: 78, tool: rule-based-clusters)"
                     /sec_met_domain="Thiopeptide_F_RRE (E-value: 3.2e-13,
                     bitscore: 39.0, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVAEMKAEQIGRAARTDMQLTVPARPVLRRGVRLRRAGESVVLDG
                     ADRAQVFSGAFAREGLVPLTEACDGTRDHTELALKTGFDEATVYKCLALLSTAGAVEEA
                     MSGEEPDVTPEWAVFLSRLGNSTGSNPSWADAAARLVSRSVRLEGDAALVAGARRSLRE
                     VCPVVTEPAGPPGPGDELTVFFETPASAPLLAATEERCRQDGRPLLRVRADARTITIGP
                     YADLSITPCLDCGRHGEADLSGEPPEYLHDLVVGLASHHVTALLARATISHLPGDFTVI
                     DTATLSTVYRPVAVRPGCPRCSYARGPVAPQAPAGAVYEASVAMPPRAFLAPKDHQAHY
                     YASNLRLQSQFKDWPSRPHTPLPALDISVLAGSERHDPSHGDTPLTLSSLGLLLKVAFG
                     VKEDETTPERVKRWTAASGNIGSTTAYAVVRDDRIMPPGVYAYAQGSHTLVTVSGEVPP
                     GDSPCDIIITGDLKKVMTKYGTFGFRLVFLDAGCNLASLRELAQHLGLGFTPRSDWDDD
                     ALARLLGTSPADEPVAAFASLGGTA"
     gene            complement(2343..2954)
                     /gene="berG1"
     CDS             complement(2343..2954)
                     /codon_start=1
                     /gene="berG1"
                     /note="YcaO-type cyclodehydratase"
                     /product="BerG1"
                     /protein_id="AGN11667.1"
                     /transl_table=11
                     /translation="MSHDPRPQCLYLVGDTFSRRLTEHRGVPPELQVSFEDFLNDTAPH
                     ADVVVPVHAGGDPGLRDETDRICAERSTPSVGLQLLPTKVLCGPVVVPGRTACYACYRK
                     RAAQHAGTARPYDMDAALSGLPEGFGRQHLSVASGLLDLALTEIATGVTGIGGTVRTFN
                     LVSGAVSSAVTVSVNRCPRCGGRFSQARADSAMPVPELLR"
     gene            complement(2978..4324)
                     /gene="berG2"
     CDS             complement(2978..4324)
                     /codon_start=1
                     /gene="berG2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters) LAP:
                     TIGR03604"
                     /gene_kind="biosynthetic"
                     /note="YcaO-type cyclodehydratase"
                     /product="BerG2"
                     /protein_id="AGN11668.1"
                     /sec_met_domain="TIGR03604 (E-value: 3.1e-103, bitscore:
                     334.8, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 8e-63, bitscore: 202.5,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLNRPQEHISAELRGLEELVSPYGLVSRTAPLPVREGEPPFAVQ
                     LAYLGVPSRALPNLRTWAHDEDTGNSDGAGTGLTPERAKLVSIAEALERYSTCAWDDDE
                     MVVAAENDLTEEFVSPSRWPSCSPTELARDDCSLSAYDPSVPIRWVRAWSLTRRIPVLV
                     PAISVYLHMPYQSKSEEFIRGITTGAAVHSDVRSAVLGGLLEVVERDAIALVWLQQLRL
                     PELVVDPARLDAGVRELHRVGTSTDLRVRLFDATTDFGVPVIYAVQLSDADPALAQIVA
                     ATCDVHPEQALGKIYRELASLRVALRGYLSAYAGREPDPAKVSVVGGAVHNATRDRRDV
                     FGFLLDGERPAYGLEGMPGLPAGADPLDTVVARLAARGAEVLVTDITTDEARQVGMRAV
                     KVLVPEAMPVSFVHGERYLGTPRLYDAPRAMGHTSHAEDAVNPVQQPFA"
     gene            complement(4371..5525)
                     /gene="berD"
     CDS             complement(4371..5525)
                     /codon_start=1
                     /gene="berD"
                     /note="putative pyridine-forming"
                     /product="BerD"
                     /protein_id="AGN11669.1"
                     /transl_table=11
                     /translation="MSYGRLQDHVTARLAPAEISGVSFVHLFATIPQPVGSKYNDTFAP
                     LIRELFAPERVGGAGGHGPYYFVRTQDAQLGTDTLQISIEGVSDEDSTRADLHRTAERY
                     GCAAQVDATPLDSVPSPLWNAGFTGTGFSASSKRLFQEAAPTLVSFLNRAAETPQSPPP
                     ALGAIRLMAAHTRATLLRSPQREIDGYEFRELLSLRLLSYRSHFEAIYLRTKDPQSFDA
                     ACARFYEQVGAGVREFITACGDPDDDPADEMVRLWTKSITSESSHLAENFSDGSVVNAG
                     HTLEDLVRKRGAPVEPTRFHTPPSPELDRLMHRDADFLAFRLQTSLLYSCLYTLGFSLA
                     ERYVFCYVVARANEDVCGKSMKELQDELDGLARSMASGSTKTAE"
     gene            5644..5787
                     /gene="berA"
     CDS             5644..5787
                     /codon_start=1
                     /gene="berA"
                     /note="berninamycin prepeptide"
                     /product="BerA"
                     /protein_id="AGN11670.1"
                     /transl_table=11
                     /translation="MEQQIELDVLEISDLIAGAGENDDLAQVMAASCTTTSVSTSSSSS
                     SS"
     gene            6110..8767
                     /gene="berB"
     CDS             6110..8767
                     /codon_start=1
                     /gene="berB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     31; E-value: 9.4e-10)"
                     /gene_kind="biosynthetic"
                     /note="lantibiotic dehydratase"
                     /product="BerB"
                     /protein_id="AGN11671.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 2.7e-32, bitscore:
                     100.9, seeds: 73, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGVNISPYVVYRRSRLPLGELGGMSFTTAWSRIDELHALRDEIGK
                     NAVGLADRLGELVPTLGDDVRADLIRLRRDVHNLRHDRAVARLEPLRPHLGREVVDEVE
                     TWCALGVRAEQCERAGREELESEKARAADGFGALFEHDAMARSIQLSGDRLYRGLRDLV
                     AGDEASALKPSKARLRESSLVNFAYRASLKPSPFGRFTEIGAFPPDDPRPADPGGRHGG
                     TQESVTTLNRLLVNWGPPGLPLVPGGMEPGHLVLNSTLRAGTEYVEYVGVAPGSREDGR
                     MATERVLRVRREGLFDALLAAMPEGSAPAATVLRDLTAVTGKAETSRKVVQGLIRAGIL
                     FFRPEIDDHDPDYSMKLDRVLAAGGTPETAALRGHFSELRRLETDFSEAAADERQKLLD
                     SAYAAIGGIAELCKVSPPPEEVLKSPVFEDTPASTAPQAWNLPTVEGSIPALTGLWRLA
                     SMMDNGQVKRLGLYSFATRVLGDRSTMPFLEFFQAFSSLTDQEQVDVFMGRDVEEAERY
                     TRQRAEALRTIRQRLVPGDGTVHLDPSVIEKACEGVEDLLDTESVTFRAQFAQGVLPDR
                     DRTLVVNGLLTGYGVYFSRFGSFVEGTDEWSLPAAQREHLARRFPGQVDLNSVLGFNFN
                     LHPSVTRRVVNYPGAVSLGAERTVYGLARLEVRADQATRSLRLWDPEAQETLDLVPMNF
                     MTPIGVPLLYRLLEALSPSNRYLWKPLDDIRDAGGPTVYGETAPRLVVGDVVADRRSWN
                     VAAAEIPMLQDLSRDVPEALVAFDAWRLTRGLPRHAFVLCQTPEERDVMAGRSRKVTRQ
                     WADYAHLRRASVHKPMYVDFRNPFLVRSFAKSALSRGDVVASIRECLPSVDDYGPDTGW
                     TAAEEFFVELCTDN"
     gene            8937..9704
                     /gene="berC"
     CDS             8937..9704
                     /codon_start=1
                     /gene="berC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: Lant_dehydr_C"
                     /gene_kind="biosynthetic"
                     /note="lantibiotic dehydratase"
                     /product="BerC"
                     /protein_id="AGN11672.1"
                     /sec_met_domain="Lant_dehydr_C (E-value: 5.6e-33, bitscore:
                     104.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLCGPGAGSAEAAERVVAGLARAMPEFGAQAREEYALGLTLQDEL
                     ARLEKETSEEGRPIGALDRVAYEPEYRKYGGTEGLQIAETVFRKSSVAVLGLLGGQPRA
                     WVDERRAPIGEAARIMAMFLHGAGLDPRAAGLFLREYEDWWRTYAPDDMQRAWPKLFGG
                     VSAQMTNLCAAVWRDGATDVFHDISAEAAARARSVCGAEPGGDVRDLRLDGTPYPGCLS
                     NYVHTTNNRLGLVPAAEGLVAYLVRRGLEAMDG"
     misc_feature    complement(9825..11045)
                     /gene="berH"
                     /note="similar to BerH; cytochrome P450"
     gene            complement(9825..11045)
                     /gene="berH"
     gene            11236..11697
                     /gene="berI"
     CDS             11236..11697
                     /codon_start=1
                     /gene="berI"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thiopeptide: thio_amide"
                     /gene_kind="biosynthetic"
                     /note="C-terminal amide-forming"
                     /product="BerI"
                     /protein_id="AGN11673.1"
                     /sec_met_domain="thio_amide (E-value: 1.4e-37, bitscore:
                     117.1, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPSVRGCVDSTLDFMCSTSWRVRLRRRSHSVCRGGVGAMYLSIV
                     MWDLKKSEATVESLREYLRDYAVDAYSALDGMRLKAWFSDSARQLWGAVYLWDSPEQMP
                     GLYKVSRVIDLIGYPPTSVGGFTLEATAEGKSVHETLAGLGIALEGGTQ"
     gene            12059..12862
                     /gene="berJ"
     CDS             12059..12862
                     /codon_start=1
                     /gene="berJ"
                     /note="23S rRNA methyl transferase; thiostrepton
                     resistance"
                     /product="BerJ"
                     /protein_id="AGN11674.1"
                     /transl_table=11
                     /translation="MASLDIITERSDSAVQRIIDVTKHSRSVVRTVLIEDIEPLLQSIR
                     AGVEFIEIYGLDTVPVPDSLLAECERRRIPVRLLAASVANQVFKTEKKPKVFGIAKVPR
                     PRRLSDLSDMTGDLILLDGVKIVGNIGAIVRTSFALGASGIVLVDSDLGSIADRRLIRA
                     SRGYVFSLPIVLASRAEALQYFQDNAMRPVVFEADGDLGVADLDGMDERLVLMFGSERI
                     GPSGEFSDIAAKSVSIPMNPAAESLNVSVSAGIALHARARRNLSR"
ORIGIN
        1 gtcagctctc cggtggccgg gcgtatgtgg tggccagcag ggaatggcgg gtcgccccgt
       61 cgctgtggac cagatggtgc acggacgacg ggacgaagcc gccgaacagt gtgccgacga
      121 gccccagcgc ctggcacctg agattgaggt cgtaggtggc catcgaggcc cgcagcgcac
      181 tgatccggta gccgtgggaa cccgcccagg tgtccgcccg gtccagactc gcgtagagcg
      241 cgacgatccc cgctccggtg gagaactcgc gctggacgcc gaggttctcg gccggtccga
      301 tctcgtcgag cccggccagg tagcacgtct cctcggcggt cacccggtac agccctggtt
      361 cggcgccctc gctgcggaac gcgaagacga agccctccag cgcgcccgcc gaggcgtcga
      421 gcccccagtc gtcacggtcc cggcgcagca cgtcacgcag cagcgacagg atcacgtccg
      481 tgcgtaccgg cagtggcgcg tagtgcagcg aggagcggcg gcgttcgagc gtgccccgca
      541 ggtcgtggac cggccgcagg tcctcggcag acggttgcgg ttcgacgggc acggagtccg
      601 cgaacggcac cggccggcgt tcggccgcca ggccgtccga cgtgggcgtc cgggaggtga
      661 agccctccag cacggccgtc atggatgtca cgtcgttcct catgcggtac ctcccaggga
      721 cgcgaaggcc gccaccggct cgtcggcagg cgatgttccg agcagccggg ccaacgcgtc
      781 gtcgtcccag tccgacctgg gtgtgaagcc caggcccagg tgctgtgcga gctcccgcag
      841 actggccagg ttgcagcccg cgtccagaaa gaccagcctg aagccgaacg tgccgtactt
      901 ggtcatgacc ttcttgagat cgccggtgat gatgatgtcg cacggcgagt cacccggagg
      961 tacctcgccg gagacggtca cgagtgtgtg gctgccctgg gcgtacgcgt agaccccagg
     1021 aggcatgatc cggtcgtcgc gcaccaccgc gtaggccgtc gtgctgccga tgtttcccga
     1081 cgccgcggtc catcgtttga cgcgctccgg agtggtctcg tcctccttca ccccgaaggc
     1141 gaccttcagc agcagtccca gggagctcag ggtgagcggg gtgtcgccgt gggacgggtc
     1201 atgccgttcc gacccggcga ggacggagat gtccagggcg gggagcggtg tgtgcgggcg
     1261 gctcggccag tccttgaact gcgactgcag gcgcaggttg gacgcgtagt agtgcgcctg
     1321 gtggtccttc ggagcgagga acgcgcgtgg aggcatcgcg accgaggcct cgtagacggc
     1381 accggcgggt gcctggggtg cgaccggccc ccgcgcgtag gagcagcgag ggcaccccgg
     1441 gcggacggca accggccggt agaccgtgct cagggtcgcg gtgtcgatga cggtgaagtc
     1501 gccggggagg tgcgagatcg tggcccgtgc gagcaacgcg gtgacgtggt gggaggccag
     1561 gccgacgacc aggtcgtgca ggtattccgg cggctcgccg gagaggtccg cctcaccgtg
     1621 acggccgcag tccaggcacg gtgtgatcga gaggtcggcg tacgggccga tcgtgatcgt
     1681 ccgcgcgtcg gcgcgcaccc gcagcagcgg acgcccgtcc tgccggcacc gttcctcggt
     1741 ggcggcgagc aggggtgccg acgcgggcgt ctcgaagaac acggtgagct cgtcaccggg
     1801 gcccggcggc ccggccggtt ccgtgaccac cgggcacacc tcccgcagcg accggcgggc
     1861 cccggcgacc agggcggcgt ccccctcgag gcgcaccgag cgcgacacca gccgcgccgc
     1921 ggcatccgcc caggacgggt tcgatccggt ggagttgccc agccgggaga ggaagacggc
     1981 ccattcgggc gtgacgtccg gttcctcgcc ggacatggcc tcctcgaccg ccccggccgt
     2041 cgacagcaag gccaggcact tgtagaccgt ggcctcgtcg aatccggtct tgagcgccag
     2101 ttcagtgtgg tcgcgggttc cgtcgcacgc ctcggtcagg ggcaccagcc cttctcgtgc
     2161 gaacgctccg gagaacacct gtgcccggtc ggccccgtcg agcacgacgg attcccctgc
     2221 cctgcgcagc ctcaccccgc ggcgcaggac gggccgggca gggacggtca actgcatgtc
     2281 cgtacgtgcg gcccgcccga tctgctcggc cttcatctcg gcaaccatga tcgatctccc
     2341 tcttaccgca gcagttccgg aacaggcatg gcgctgtccg cgcgcgcctg cgagaaccgg
     2401 cccccgcacc gggggcaccg gttcaccgag accgtcaccg cggacgagac ggctccggag
     2461 acgagattga acgtacgtac ggtgcccccg atccccgtca cacccgtggc gatctcggtc
     2521 agggcgaggt ccaggaggcc ggaggcgacg gacaggtgct gccggccgaa cccctcgggc
     2581 aggccggaca gggcggcgtc catgtcgtac gggcgggcgg tgcccgcgtg ttgggcggcg
     2641 cgcttcctgt agcacgcgta gcaggccgtc cggcccggaa ccacgacggg tccgcagagg
     2701 accttggtcg gcagcagctg gagcccgacg gacggtgtgc tccgttccgc gcagatccgg
     2761 tccgtctcgt cgcggagccc ggggtcgccg ccggcatgca cggggacgac gacatccgcg
     2821 tgcggtgccg tgtcgttcag gaagtcctcg aaggacacct gtagctccgg ggggacacca
     2881 cggtgctccg tgagccgccg gctgaaggtg tcacccacga ggtacaggca ctgcggacgc
     2941 ggatcgtgcg acatgctgct ctgctccttc ttctcgctca tgcgaacggc tgctgcacgg
     3001 ggttgaccgc gtcctcggca tggctcgtgt gccccatcgc gcgcggcgcg tcatagaggc
     3061 gcggggtgcc caggtaccgc tcgccgtgta cgaacgacac gggcatggcc tcgggcacga
     3121 gcaccttgac cgcccgcatg cccacctggc gcgcctcgtc cgtggtgatg tccgtgacga
     3181 gcacctcggc accccgtgcc gcgagccggg cgacgacggt gtccagcggg tccgctcccg
     3241 ccggaagccc cggcatgcct tcgaggccgt acgccggccg ttcaccgtcc agcaggaagc
     3301 cgaacacgtc ccgccggtcg cgcgtcgcgt tgtggaccgc gccgccgacg acgctcacct
     3361 tggccggatc gggctcgcgc ccggcgtacg ccgagaggta tccgcgcaac gccacccgca
     3421 gggaggccag ttcgcggtag atcttgccca gcgcctgttc cggatggacg tcgcaggtgg
     3481 cggcgacgat ctgggcgagc gcggggtcgg cgtcggagag ctgcacggcg tagatcaccg
     3541 ggacgccgaa gtccgtcgtc gcgtcgaaca gcctcacccg caggtccgtg gaggtgccga
     3601 cgcggtgcag ttcccggacg ccggcgtcga gccgcgccgg atccacgacc agctcgggca
     3661 gccgcagctg ctggagccag accagcgcga tggcgtcccg ctcgacgact tccagcaacc
     3721 cgccgagcac ggcgctgcgt acgtcggagt ggacggcggc acccgtggtg atcccgcgga
     3781 tgaactcttc ggacttcgac tggtagggca tgtgcagata cacggagatc gccggcacga
     3841 gcaccggtat ccgccgggtg agcgaccatg cgcgcaccca tcggatcggg accgaggggt
     3901 cgtacgcgga caggctgcag tcgtcgcggg ccagctcggt gggcgagcag ctgggccacc
     3961 gggacggcga cacgaactcc tctgtgaggt cgttctcggc ggcgaccacc atctcgtcgt
     4021 cgtcccaggc acacgtggag taccgttcga gcgcttccgc gatggagacc agcttggcgc
     4081 gctcgggcgt gagcccggtg cccgcgccgt cggaatttcc ggtgtcctcg tcgtgggccc
     4141 acgtccgcag gttgggaaga gcacgggacg ggacaccgag ataggccagc tggacggcga
     4201 agggcggttc gccctcgcgc accggcagcg gcgcggtgcg tgacaccagt ccgtagggcg
     4261 agacgagctc ctcgagaccg cgcagctcgg ccgagatgtg ctcctgaggc ctgttcagat
     4321 gcatgtggtt cttcttcccg cgtccggatg ttccccgtgt ccttgccgcg ctactcggcg
     4381 gtcttcgtgg atcccgacgc catgctcctg gccaggccgt cgagctcgtc ctggagctcc
     4441 ttcatggact ttccgcagac gtcttcgttc gcacgtgcga cgacgtagca gaagacgtac
     4501 ctctccgcca ggctgaaacc gagggtgtac aggcagctgt acagcaggct ggtctgcagc
     4561 cggaaggcca ggaagtcggc gtcgcggtgc atcaggcggt ccagctcggg gctcggtggc
     4621 gtatggaagc gcgtcggctc gaccggggcg cctcgcttgc gaacgaggtc ttcgagcgtg
     4681 tgtccggcgt tgacgacgga tccgtccgag aaattttcgg ccaggtggct tgattcagac
     4741 gtgatggatt tcgtccaaag ccgaaccatc tcgtcggccg gatcgtcgtc cggatcgccg
     4801 cacgccgtga tgaattcccg gacgccggcc cctacctgtt cgtagaaccg ggcgcaggcg
     4861 gcgtcgaagg attgcggatc cttggtgcgc agataaatcg cctcgaaatg cgaacggtaa
     4921 ctcagcaggc gtagggaaag cagctcacgg aattcgtatc cgtcgatttc tctctgcggt
     4981 gatctcagaa gagtggctct ggtgtgcgcg gccatgaggc ggatggcacc gagtgccggc
     5041 ggaggcgatt gaggtgtctc tgccgcacga ttcaagaatg agacgagagt cggtgcggcc
     5101 tcctggaaga gccgcttgga ggaagcggag aatccggttc cggtaaaccc cgcgttccac
     5161 aacggtgagg gaaccgaatc aaggggcgtg gcgtccacct gtgcggcgca cccatatcgt
     5221 tcggccgttc tatgcaggtc agcgcgggtg gagtcctcgt cggacacccc ttcgatgctg
     5281 atctgcaggg tgtccgtgcc gagctgcgcg tcctgggtgc ggacgaagta gtaagggccg
     5341 tgaccgcctg ctccacctac ccgctccggc gcgaacaatt cgcgaatcag gggcgcgaac
     5401 gtgtcgttgt attttgaacc aactggctgc gggatggtgg caaacaagtg cacgaatgaa
     5461 acccccgaga tttcggccgg cgccagacgg gccgtcacgt gatcttgtaa tcgaccgtaa
     5521 ctcagagaga tgagaagaag gaaccccggc tccagtgtga tctgcgtcac tccggaaact
     5581 gcttgcgttg tgatcgtccg aatgcctaga ttcgaatcac attgacgaaa gggggtgtaa
     5641 tcaatggagc agcagatcga actcgatgtg ctcgagattt cggacctcat tgcaggtgcc
     5701 ggggagaacg atgacctggc gcaggtgatg gccgcctcgt gcacgaccac cagtgtttcg
     5761 acgagttctt cgtcgtcctc gtcctgaatc tagggaccgg gaaacagttg agccaccgtc
     5821 ggggtgttcc tcggcggtgg ccttctgcag tcctggcgtg cctttttcga ttcgaggaac
     5881 gcgccgtccc tggaggtcga ccagaggtgc gggcgtcgtg ccccggacga cgacgggctc
     5941 gtgccaagcc ggccggccga gagccggaac acatgttgtc aagcgcgaac tgaccggtgc
     6001 ggtggagtga caggcgggct ccgtgcggcg gggcgcctct ccgcggcccg gccccgacgt
     6061 ccgcacgccg tggtgaaccg ggccggcgtg acgaagttgg gggattccta tgggtgtgaa
     6121 catcagtccg tacgtcgtct atcggcgcag cagactccca ctgggcgagc tcggagggat
     6181 gtccttcacc accgcctggt cgcgcatcga tgaactgcac gccctgcggg acgagatcgg
     6241 caagaacgcc gtcggcctgg ccgaccgcct cggcgagctc gtgcctacgc tgggggacga
     6301 cgtccgggcc gacctgatca ggctgcggcg cgacgtgcac aatctgcggc acgaccgggc
     6361 ggtggcgcga ctggagccac tgcgtccgca tctcggccgc gaggtggtcg acgaggtcga
     6421 gacctggtgc gcgctcggcg tgcgggccga acagtgcgag cgagcagggc gcgaggagct
     6481 cgagagtgag aaggcccggg ccgccgacgg cttcggcgcc ctcttcgagc acgatgcgat
     6541 ggcgcgcagc atccaactct ccggcgaccg gctgtaccgg ggcctgcgcg acctcgtcgc
     6601 gggcgacgag gcgagcgccc tcaagccgag caaggcccgg ctgcgggagt cttccctcgt
     6661 caacttcgcc taccgggcga gcttgaagcc gtcccccttc ggacggttca ccgagatcgg
     6721 cgcgttccct ccggacgacc cgcgccccgc ggatcccggt ggccggcacg gcgggacgca
     6781 ggagtcggtc acgacgctga accgtctcct cgtgaactgg gggccccccg gcctgccgct
     6841 cgtaccgggc gggatggagc cggggcacct cgtgctgaac tccacgctgc gggccggcac
     6901 cgagtacgtc gagtacgtcg gtgtcgctcc cggctcccgt gaggacggcc ggatggccac
     6961 cgagagggtg ctgcgcgtac gccgggaggg actcttcgac gcactgctcg cggcgatgcc
     7021 cgaaggatcg gctccggcgg ccacggtgct gcgcgacctc accgccgtca ccgggaaggc
     7081 ggagacgagc cggaaggtcg tgcaggggct gatccgggcc ggcatcctct tcttccggcc
     7141 ggagatcgac gatcacgacc ccgactactc catgaagctc gaccgcgtac tcgcggccgg
     7201 cgggacgccg gagacggccg cgctacgcgg acacttctcc gaactcaggc ggttggagac
     7261 ggacttctcc gaggcggcgg ccgacgagag gcagaagctg ctcgactcgg cgtacgcggc
     7321 gatcggcggc atcgccgagc tgtgcaaggt gtccccgccc cccgaggagg tcctgaagtc
     7381 accggtcttc gaggacactc cggcatccac ggcgccccag gcctggaacc tgccgacggt
     7441 ggaggggagc atccccgccc tgacgggcct ctggcgtctg gcctcgatga tggacaacgg
     7501 ccaggtgaag cgactgggtc tctactcctt cgccacccgc gtgctcggcg accgcagcac
     7561 gatgcccttc ctcgagttct tccaggcctt ctcgtcgctg acggaccagg aacaggtcga
     7621 cgtgttcatg gggcgcgacg tggaggaggc cgagaggtac acgaggcagc gggcggaggc
     7681 tctgcgcacg atccggcagc ggctggtgcc cggggacggc accgtgcacc tggacccctc
     7741 ggtcatcgag aaggcctgcg agggcgtgga ggacctcctc gacacggaat cggtgacgtt
     7801 ccgcgcgcag ttcgcccagg gagtgctgcc cgaccgggac cggacgttgg tcgtgaacgg
     7861 cctgctcacc ggctacggcg tctacttctc acggttcggc tcgttcgtcg agggcaccga
     7921 cgaatggtcc ctgccggccg cccagcggga gcacctcgca cgcaggttcc ccggccaggt
     7981 cgacctcaac tccgtgctcg gattcaactt caacctgcac ccctcggtga cccggcgggt
     8041 cgtcaactac cccggcgcgg tgtcgctcgg cgccgagcgg acggtctacg gactggcgcg
     8101 tctggaggtc cgcgcggatc aggccaccag gtcgctgcgc ctctgggacc ctgaggcgca
     8161 ggaaaccctc gacctcgtgc ccatgaactt catgaccccg atcggggtcc cgctgctcta
     8221 ccgtctgctc gaggcgctgt ccccgtccaa ccgctacctg tggaagcccc tggacgacat
     8281 cagggacgcg ggagggccca cggtgtacgg cgagacggca ccccggctgg tcgtgggtga
     8341 cgtcgtggcc gaccgcaggt cctggaacgt ggccgcggcc gagatcccca tgctccagga
     8401 tctgagccgg gacgtgcccg aagcgctcgt ggccttcgac gcgtggcgcc tgacgcgggg
     8461 ccttccccgc cacgccttcg tgctgtgcca gacgcccgag gagcgagacg tcatggccgg
     8521 gcgcagccgg aaggtgaccc gccagtgggc ggactacgcg cacctgcggc gcgccagcgt
     8581 gcacaagccg atgtacgtcg acttccggaa ccccttcctg gtccggagct tcgcgaagtc
     8641 ggccctgtca cgcggcgatg tcgtcgcgtc gatccgcgag tgccttcctt cggtggacga
     8701 ctacggcccg gacacgggct ggaccgcagc agaggagttc ttcgttgaac tgtgtaccga
     8761 caactagtgg gcagaccgga acgcgtgagt ggaggacggt ccacatccac gttccgcact
     8821 cgctgcacac ccccttcctg tgcgacgtgg tcgagccgct gctccggtcc gagggactcc
     8881 aggaccactt cttcttcctc cggtactggc agggcggccc ccatctgcgg ctgcggatgc
     8941 tctgcggccc cggggccggt tcggccgagg cggccgaacg ggtcgtcgcg ggtctggcac
     9001 gtgcgatgcc ggagttcggt gcgcaggcgc gggaggaata cgcgctcggg ctgaccttgc
     9061 aggacgagct cgcccgcctg gagaaggaga cctcggagga gggccggccc atcggggccc
     9121 tcgaccgggt ggcgtacgag ccggagtacc gcaagtacgg gggaacggag gggctgcaga
     9181 tcgccgagac cgtattccgc aagtcgtcgg tggcggtcct cggcctgctg ggcgggcaac
     9241 cgcgggcgtg ggtggacgag cgccgggcac cgatcgggga agccgcgagg atcatggcga
     9301 tgttcctcca cggcgcaggc ctcgacccgc gggccgcagg gctgttcctg cgggagtacg
     9361 aggactggtg gcgtacgtac gcgccggatg acatgcagcg tgcctggccg aaactgttcg
     9421 gcggcgtctc ggcacagatg acgaatctgt gcgcggcggt ctggcgtgac ggcgccacgg
     9481 acgtgttcca cgacatcagc gcggaggccg ccgcccgcgc ccgttccgtg tgcggggcgg
     9541 agcccggcgg cgatgtccgc gacctccggc tcgacggcac gccttacccg ggctgtctct
     9601 cgaactacgt gcacaccacc aacaaccgtc tcggcctggt ccccgccgcc gaggggctcg
     9661 tcgcgtacct cgtgcgccgg ggcctggaag cgatggacgg gtagggcctg tcgttgtcgg
     9721 gatcatgcgg ggccccgatg ccccggtgcc ggaagcccgg agcctccgtc ccgcgcaggc
     9781 ccttccctgt ggggcctgcg ccggacggag agctcaccgg tgcgtcacca gctcaccgga
     9841 agcttgtgca ggccgtacac ccccatgctg tccttgaacg gcagttccgc gaccggggtg
     9901 gccagcttca gtccgggaat cctcgcgagg agggcgttga agacgacctc cagctccagt
     9961 ttcgcgaggt tctggccgat gcactgatgg atgccgtggc cgaacgccag gtggtgcctg
    10021 ccgccgcgct cgatgtccag cctgtccggg tcggggaaca ccgcctcgtc gtggttgccg
    10081 gaggcgctga gcccgatgac gccctcaccc gcgcggatca gcaccccgcc gatctcgagg
    10141 tcggccgtcg ccacccggga ggtcacctgg tcggcgatgc tgaagtagcg cagcagctcg
    10201 tcgacggcct gcggggccag acccgggtcg gccctgagct tggccagctg gtcggggttc
    10261 tccagcaggc ccacgacgcc gagggagatc atgttcgcgg tggtctcgtg accgccgacc
    10321 aggaggacgt tcgccatccc gaccagttcc ccgtggtcga aggtgccggt ctcccggttc
    10381 ttcacgacga gcctgccgag cagatcgtcc cccgggtccg cttcctgagc ggtgaccagc
    10441 tcggagaagt acgcgtggag ctcttgatgc gcgctgttgc gctcctgtgg gtcggcgtcg
    10501 accgacacca gcttgttggt gcggtcctgg aagaacgcgc ggtcgctgcg gggcaccccg
    10561 agcagctcgc agatcaccag ggacggcacc gggagggcga gcgcctcgac cagatcggcc
    10621 ggcccctcgc cggcgagcat ggaggagatg cactcgtcca cgatctcctc gacgcgggga
    10681 cgcagttgga gcacccgctt gacggtgaac tccgggatca ccatcttgcg ctgcgccgtg
    10741 tgtacgggcg ggtccatggc gagcagcacg ggccgcatct gctccatgac ctccggcggc
    10801 gcatcgaagt ggagtggata gcccgggtgg gccaggttcg agctcacatg agggtcggcg
    10861 agcaactgtc gtatgtgctc gtgccgggtg agcagccacg cggtccgccc ggaggccaga
    10921 gtgaccttgg tgatcggctg ctcggcacgc agggacgcgt actccttcgg cgggtggagc
    10981 gggcaggtcc tggggtaggg atacgcgctg tcctgtcggt ccgtcacggt cttctccgcg
    11041 ggtagtccag ggaaatctcc ttacggactc cattcaagcg gaagatgatc cacatcgtgg
    11101 cgttatcggt gaatgaggcc gaactcacgt ggaccgcagg gaggaaagtc ggccgccttc
    11161 ccggcatggc ccgtcccgtg ctttcggtgg gaggggtcgg tgcagtgcga cattgcagtg
    11221 atcgcgtaat ccggaatgac cccttccgtg cggggatgcg tcgatagtac gttggatttc
    11281 atgtgctcca catcgtggag agttcgcttg cgccgtcgaa gtcacagtgt gtgccgaggg
    11341 ggagttgggg ctatgtacct ttcgatcgtc atgtgggacc tgaagaagtc ggaagccacg
    11401 gtggagagcc tcagggaata cctgcgggac tatgccgtgg acgcctactc cgcgctggac
    11461 ggaatgcggc tcaaggcgtg gttctccgat tccgcacgtc agctgtgggg tgcggtctat
    11521 ctgtgggaca gccccgagca gatgcccggc ctgtacaaag tcagccgcgt gatcgatctc
    11581 atcgggtatc cgccgacttc ggtcggtggt ttcacgctcg aggcgaccgc cgaagggaag
    11641 agcgttcacg agacactggc cggcctgggg atcgccctgg agggcggaac gcagtaaggg
    11701 caggcgtgcg accggatggg cgggaggttt gccgggccgg tcggttcgag gggcggtcga
    11761 gggccggagc cgcgcgggtg gcttcgccgc tccggctggc ggccgggtgt ggagtgccgc
    11821 gcggcgccgg tgtcgcccgc gccgggcatc accgtgctcg cggagggttc aggtgtgtcg
    11881 gaccggcgct ttcgcgtgtg ttcggcaccg tccggtgggc cgggtgctgt gcgcgggcct
    11941 cgagctcccg ggcgggcgcg gtgcgcaggc cctccccgcc cggacacgcg tattccgcac
    12001 cgcttcacga agatcattcg gtgaaggagg cgggggcgct cgtgctaacg tcgtgatcgt
    12061 ggccagcctt gacattatta ccgaacgctc tgattctgcc gtacaacgca tcatcgatgt
    12121 gacaaagcat tcgaggtccg ttgtccgcac ggtgctgatc gaggacatcg agcctcttct
    12181 gcagagcatc cgtgccggag tggaattcat cgagatctac ggactcgaca ccgtgcctgt
    12241 tccggacagt ctgctcgccg aatgtgaacg gcgcagaatt ccggtccggc tgctcgccgc
    12301 ttcggtcgcc aatcaggtct tcaagaccga gaagaagccc aaggtattcg gtatcgccaa
    12361 ggtcccgcgg cctcgtcgcc tgtcggacct gtccgacatg accggtgacc tcatcctgct
    12421 cgacggagtg aagatcgtcg gcaatatcgg agccatcgtg cggacctcgt tcgcgctcgg
    12481 ggcctcggga atcgtgctcg tggacagcga tctcggcagt attgcggacc gccgtctgat
    12541 cagggcgagc cggggctatg tgttctccct tcccatcgtt ctcgcgtccc gggccgaggc
    12601 gctccagtac ttccaggaca atgcgatgcg cccggtggtg ttcgaggccg acggggatct
    12661 cggcgtcgct gatctcgacg gtatggacga gcgacttgtg ctcatgttcg gcagcgagag
    12721 gatcggcccg tcgggcgagt tctccgacat cgccgccaag tcggtctcca ttccgatgaa
    12781 tcccgcggcc gagtccctca acgtatcggt gtcggccgga atcgcgctgc acgcgagggc
    12841 ccgccgtaac ctctcccggt ag
//
