Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0001479.1:
Cluster 1NRP16546

BGC0001479, anabaenopeptin NZ 857 / nostamide A biosynthetic gene cluster from Nostoc punctiforme. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: anabaenopeptin NZ 857
PubChem ID: 133562467
SMILES string: Copy to clipboard
Molecular formula: C45H59N7O10
Average molecular mass: 858.006 Da
Molecular activity: Inhibitor
Molecular target: Protease

Class-specific details

Biosynthetic class(es):
NRP
Other (cyclic)
Thioesterase type:
Type I
Release / cyclization type:
Hydrolysis

Nonribosomal peptide synthetases:
aptA (ACC81021.1)
Module 0
A specificity: Phenylalanine
Evidence for specificity: Structure-based inference
C domain subtype: N/A
Module 1
A specificity: D-lysine
Evidence for specificity: Sequence-based prediction
C domain subtype: Dual
aptB (ACC81022.1)
Module 2
A specificity: Isoleucine
Evidence for specificity: Structure-based inference
C domain subtype: DCL
aptC (ACC81023.1)
Module 4
A specificity: N-methylglycine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module A3
A specificity: Homophenylalanine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Scaffold-modifying domain: Methylation
Module B3
A specificity: Homotyrosine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Scaffold-modifying domain: Methylation
aptD (ACC81024.1)
Module 5
A specificity: Homotyrosine
Evidence for specificity: Structure-based inference
C domain subtype: LCL

Gene cluster description

anabaenopeptin NZ 857 / nostamide A (BGC0001479). Gene Cluster 1. Biosynthetic class = NRP. GenBank CP001037, positions 3052233-3081156. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Paul D'Agostino (Technical University of Munich)

Literature references

1. Rouhiainen L et al. (2010) Two alternative starter modules for the non-ribosomal biosynthesis of specific anabaenopeptin variants in Anabaena (Cyanobacteria). Chem Biol 17(3):265-73. doi: 10.1016/j.chembiol.2010.01.017.
2. Greunke C et al. (2018) Direct Pathway Cloning (DiPaC) to unlock natural product biosynthetic potential. Metab Eng 47:334-345. doi: 10.1016/j.ymben.2018.03.010. Epub 2018 Mar
3. Koketsu K et al. (2013) Identification of homophenylalanine biosynthetic genes from the cyanobacterium Nostoc punctiforme PCC73102 and application to its microbial production by Escherichia coli. Appl Environ Microbiol 79(7):2201-8. doi: 10.1128/AEM.03596-12. Epub