LOCUS       BGC0001480             19784 bp    DNA     linear   CON 13-DEC-2022
DEFINITION  Methylobacter tundripaludum 21/22
            T451DRAFT_scf7180000000002_quiver_dupTrim_4432.1_C, whole genome
            shotgun sequence.
ACCESSION   BGC0001480
VERSION     BGC0001480
KEYWORDS    .
SOURCE      Methylobacter tundripaludum 21/22
  ORGANISM  Methylobacter tundripaludum 21/22
            Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
            Methylococcaceae; Methylobacter.
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            JMLA01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: not full length.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:39:13
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 19784
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     subregion       1..19784
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001480"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..19784
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /subregion_numbers="1"
                     /tool="antismash"
     protocluster    1..7637
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1938:2637]"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1939..2637
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     cand_cluster    1..7637
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     gene            25..696
                     /locus_tag="T451_RS0104065"
     CDS             25..696
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /locus_tag="T451_RS0104065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HAD-IB family hydrolase"
                     /protein_id="WP_160171839.1"
                     /transl_table=11
                     /translation="MEVCAFFDVDETIWNEKSVITFYQFYLDVTKGDKADFFWEQFNKN
                     VKNTISAGISREELNAWFYLEHFSGVSVSHLREIAEQWSIKKFNQPSFWQDKVIERIEY
                     HKNKGHAIVLVSGSFREVLAPLAKRLGVTDILCSPLEEKDGCYTGNMLGSPMIGYGKAN
                     AVSDYLREHKIDPVHCYGYGDDTTDIPFIGTVGNSYIVINNNHDELSVLAEIMGYQTLN
                     V"
     gene            745..1896
                     /locus_tag="T451_RS0104070"
     CDS             745..1896
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012702.2"
                     /locus_tag="T451_RS0104070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_027148169.1"
                     /transl_table=11
                     /translation="MMSLENLLGDDNAASIKRQLDHAPFRRLSEKNETYIKPEWLCQAF
                     HINRLTGEPKLILGDQVGGYYMHSIESIKPSVDKMLNQNIKRVYLGTDAVIGEEGSYLA
                     KLNDFANILVSLRTIVGPDVEIIVDPAGLCLRKDLRWGVTAEGGDINAQETLALLAQAA
                     VTFEEAGANALLTIGRSNCEVAAVKQALQSKNKDMRVLSFSTNSETTSAYFEVTQHDIL
                     RSRTGQKIFVGNIEEMLVRAICDFGEGSDVIVQKPVESFHLPAILRLLSEGLISFESLM
                     DNSETIDILLNNNPHIRPAFNAGVELLKSKKRILKTGTYEVSGTYSTIQLIINRYSEQL
                     GWSMLDEILLNAASAAGKSLDIMISRNATWYLEKRGLYSIKES"
     gene            1939..2637
                     /locus_tag="T451_RS0104075"
     CDS             1939..2637
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015700.2"
                     /locus_tag="T451_RS0104075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="WP_027148170.1"
                     /sec_met_domain="AfsA (E-value: 2.7e-13, bitscore: 40.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKKMILVGDQFKEFANEESVLTLTHFRSAYQQDKNINSRIVVGQ
                     GLSHDEVNHWRNSGLNVEAPSNPASLKVTHKHHLDHVMISEPKKGEAFNYQMDLMLNDE
                     TDRLLDHITSLHVSGMVLIEAARQASIACTEIEYDFLNREKPSGYTWTNMNVRFSQFIF
                     PVPTRIELEIVERENNEKRFTLSSKVNFIQGDNAVCDMEMMFDVLPLKLINQIERRSSA
                     KIIRNLTLTD"
     gene            2639..4234
                     /locus_tag="T451_RS0104080"
     CDS             2639..4234
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050:monooxygenase FAD-binding (Score: 228.6; E-value:
                     2.6e-69)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /locus_tag="T451_RS0104080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_027148171.1"
                     /transl_table=11
                     /translation="MKIDTHIVKVLIVGAGPTGLTLAACLEKFGISPDIVDFKNCPNDQ
                     SKALAINPVAQAQLGIIFGKNNVGKNSNIVTKLKVIFNTDKRLTTIDLKHLGWPCNSML
                     VQSQYDTEKDLIELAKNNSVNVRWETRVVEVIEHDDAVQVVFEGPDNQTIKQQYDYVIG
                     CEGKQSLVREAIGATMTPLPYSMYLALADFKLDIDLSEDSAYYFVYEDTFFVFVPLGKS
                     IWRIVVKHNGAPKRGEYINLVTDPVLEKFGRNIFCGECLWFSQAPLYVSYANKLQSKRL
                     FIAGDSAHLFSPISGTGMNTGIVDAVNLAWKIAFTIKGNACGNTLIQSYQEERIPAIKE
                     NAMATDRLTKLISRQEEHTDQFMPKMSNRAYIRNLFPLNVSGFGFSYPSSPVLFGKTDD
                     PSIGRMDSKLTKLLMSSSEFIPDFSLYINVLHFLDQDLNKTVLPCPKQTRLKGVRDVVV
                     QWINNDEVVDSSCMQVQQSPNIINIALLKKDWQELRQLGDLQVLRPDGIVVFRGSIKEH
                     GEVVINLSEYLNLQ"
     gene            4248..5237
                     /locus_tag="T451_RS0104085"
     CDS             4248..5237
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039:aldo/keto reductase family oxidoreductase (Score:
                     311.9; E-value: 7.1e-95)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890613.1"
                     /locus_tag="T451_RS0104085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aldo/keto reductase"
                     /protein_id="WP_027148172.1"
                     /transl_table=11
                     /translation="MNNKNILSNAIRIGLGCMGMSEFYGETDDDNSLDILHAAYDLGYR
                     HFDTADMYGKGHNERLIGTFVKELGSRGKDILIATKVGIKRDVNGPGTLVIDSTPEYIL
                     QACEQSLNRLGVEQIDLYYLHRRNPDVPIEETMGAMKQLLDEGKIAAVGLSEVSTETLK
                     QANSIVKVSALQSEYSLWSRDIEDSILPACNELNINLVAYSPIGRGFLSGTLKKEQVHN
                     EGDLRGKLPRFQQESFENNQKLLDAVKHVADEKNCSLAQVALSWLLGQNPLVAVIPGAR
                     KLNHLIDNFASQDIHLSKEQISYLSEAFKPDNVSGYRYPEPLLKTTNT"
     gene            5250..6791
                     /locus_tag="T451_RS0104090"
     CDS             5250..6791
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 334.5; E-value:
                     1.3e-101)"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /locus_tag="T451_RS0104090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MDR family MFS transporter"
                     /protein_id="WP_027148173.1"
                     /transl_table=11
                     /translation="MYAKAENKWLVAASVTTGAVMAAVDTSILNIATPHLRGVFSATTA
                     EISWISTGYLLAVVIGMPLTGWLCSQFDRKNVCLGGLAVFIFASILCGFATELGLLVAA
                     RILQGLGAGVLLPVEQVILRQAFPPKEHGLAMGLYGVTVMIGPTIAPLLGGLIIDNYHW
                     SLIFFVNVPVGLAGVFMVHRFVQNKPAQGDPVVSIKPDWIGILLLAMALFFLLWLLERG
                     ERLDWFETQSNVWLLVVSISSFTLFCAHELITKKPAVDLRILNNRAFSSAVFMSFVLGF
                     VVSATLFVLPIYMQDVLGFSATHAGEALVPRALVMMIAFPLIGRLYNHVSTKLLMAIGL
                     ALGFFSAILMSKFTKVTGIHDIILPQILQGVAVSCILTPLSTVALLHVAADRLAAAAGL
                     NAFSRQLGSSIGIAICSSLISHFELVVRGALIHHINWANPVLRDRFTGVIRYFYTKSIN
                     DYTTAMHQGFSQLNSNVTKQVVVISYQKTFEWLAVAFIVMFICLFFMKTKRHEYI"
     gene            6870..8045
                     /locus_tag="T451_RS23295"
     CDS             6870..8045
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029:RND family
                     efflux transporter MFP subunit (Score: 130.5; E-value:
                     1.2e-39)"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /locus_tag="T451_RS23295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HlyD family secretion protein"
                     /protein_id="WP_051604059.1"
                     /transl_table=11
                     /translation="MINKLKTDYRIWAGSIVIIFCSLLAGWQSINFDKESTDNAIVRCD
                     VVDVTSQVSGIIQSVHFHDNQLVNVDDDLINIDPDLYRARLDRAEARLNIAELSYSAAL
                     KDELLSEINAKSDFERSKDSLDAALANLEAIKSQMDEAKASSRSAKVDLKLAEANYQRV
                     LSIFQKGMTSKASFDNAESNMDSKKALLAASQANVEAQENQFGAEKARVAEIRKRSNVY
                     GQSEGSLISKAQANTEISSGNVKVARAERDLAALDYSRTKIKAFRSGIITNRKVALGHY
                     VEANQPIGSIVTCTEKAWIEANYKETQVGRMKPGQPVKFTVDAYPDVEFLGEVESISGG
                     SGAIFSLLPPENATGNFTKVVQRIPVLIKIDQEQNVKMRVGMSVVATVITE"
     gene            8066..8308
                     /locus_tag="T451_RS0104100"
     CDS             8066..8308
                     /NRPS_PKS="Domain: PP-binding (18-71). E-value: 4.4e-11.
                     Score: 35.1. Matches aSDomain:
                     nrpspksdomains_T451_RS0104100_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /locus_tag="T451_RS0104100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl carrier protein"
                     /protein_id="WP_006890610.1"
                     /sec_met_domain="PP-binding (E-value: 1.4e-11, bitscore:
                     35.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEIEKNLLNALRQSMPKPIADRVIEADSNLSFDLGIDSLGLMTLS
                     VNIENAYAINLVDHVEALMTVKSVSELQTLIHSVL"
     aSDomain        8120..8278
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_T451_RS0104100_PP-binding.1"
                     /evalue="4.40E-11"
                     /label="T451_RS0104100_PP-binding.1"
                     /locus_tag="T451_RS0104100"
                     /protein_end="71"
                     /protein_start="18"
                     /score="35.1"
                     /tool="antismash"
                     /translation="IADRVIEADSNLSFDLGIDSLGLMTLSVNIENAYAINLVDHVEAL
                     MTVKSVSE"
     gene            8317..9630
                     /locus_tag="T451_RS0104105"
     CDS             8317..9630
                     /NRPS_PKS="Domain: Condensation_DCL (27-295). E-value:
                     1e-12. Score: 39.6. Matches aSDomain:
                     nrpspksdomains_T451_RS0104105_Condensation_DCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="T451_RS0104105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_027148175.1"
                     /sec_met_domain="Condensation (E-value: 4.7e-15, bitscore:
                     45.6, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKIRELGFGELRMHLLHSLGDGTTQGALHVRIEGELDDKYLESV
                     VETLLIKLPVLRATIQKIDGRYYFCLADRNHHRNGFLDITVDKNAPNLWQARLEAENNL
                     LLNSEEQLWRLVAVNYQSVNCWDLILVMHHALMDAAGSEFLLDQILGCLGNLYEIGSLE
                     STDNVFPSLEEDTVTACEWEKYVGIQSNIKIKLASLNLQKHLSSVGLNERNTKSQYFQV
                     NQDLVAQLEDFCEINQISLNSLFSAALLSSIALCTPDRKSFALFSAVSLRPLCQNVKER
                     SLGCYLNVMPTVHSFSDDSVSSIISLSREHKIEVQRAFLNTGRWIPRNYDVAIVENMIS
                     TLANSNTFGNDIGITYAESGLAPQYGKLKVKHQYVTARRNIGNVALILHGLKSDRDIYF
                     SLSHVEPLQSREWAILVKNKFLDLLLMISKSSFDTVKI"
     aSDomain        8398..9201
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_T451_RS0104105_Condensation_DCL.
                     1"
                     /domain_subtypes="Condensation_DCL"
                     /evalue="1.00E-12"
                     /label="T451_RS0104105_Condensation_DCL.1"
                     /locus_tag="T451_RS0104105"
                     /protein_end="295"
                     /protein_start="27"
                     /score="39.6"
                     /tool="antismash"
                     /translation="ALHVRIEGELDDKYLESVVETLLIKLPVLRATIQKIDGRYYFCLA
                     DRNHHRNGFLDITVDKNAPNLWQARLEAENNLLLNSEEQLWRLVAVNYQSVNCWDLILV
                     MHHALMDAAGSEFLLDQILGCLGNLYEIGSLESTDNVFPSLEEDTVTACEWEKYVGIQS
                     NIKIKLASLNLQKHLSSVGLNERNTKSQYFQVNQDLVAQLEDFCEINQISLNSLFSAAL
                     LSSIALCTPDRKSFALFSAVSLRPLCQNVKERSLGCYLNVMPTVHS"
     gene            9642..11243
                     /locus_tag="T451_RS23300"
     CDS             9642..11243
                     /NRPS_PKS="Domain: AMP-binding (34-423). E-value: 5.4e-42.
                     Score: 135.6. Matches aSDomain:
                     nrpspksdomains_T451_RS23300_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     102.8; E-value: 2.6e-31)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /locus_tag="T451_RS23300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AMP-binding protein"
                     /protein_id="WP_027148176.1"
                     /sec_met_domain="AMP-binding (E-value: 3.9e-44, bitscore:
                     141.0, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQYTILDLHKRIATNSTNCLTFFNKQGELISKPYTEVFRDIRKT
                     LIHMREFDIANSGTRFVVCGEPGYHWICAALAAIFNGAQLVALPESMSEEEILASIDGL
                     SIESALVADKYKASKAFASVKTFELSNFCEQISSIDCDDDIVLSFMVSEKVIAFTSGST
                     AEAKFKAFEVPLQTSIAFTNRFRKLYSINKEDNWLICHSFSHIVHFEYLLGGLYWGYNV
                     TIATAFQILMKGASFNPSVLVTVPSVYEQIASQIRNKFRLADDGENRLHALESRPISNE
                     NGDVIRYPLCEEAKLVLGDKLKMLLIGASPSSLELQTFLLQTGLPLYEGYGMTELNMIS
                     CNTPEKSKLSTVGPAWPGIEIKLDDDNCILARATPERARFYLNANQETNQSTFLDNGWI
                     NTQDLGRIDDGVLRIVGRKKDIIVNAGGKNISPSSIETKLTSIPTVNHAVVYGDMKPYL
                     VAVLSSTEEDRFLLEDIQCSLDRINTSLPLHERIMGFILSEEPFTVESGTLTRLGKVRR
                     TKVIERYQDELEKLYA"
     aSDomain        9744..10910
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_T451_RS23300_AMP-binding.1"
                     /evalue="5.40E-42"
                     /label="T451_RS23300_AMP-binding.1"
                     /locus_tag="T451_RS23300"
                     /protein_end="423"
                     /protein_start="34"
                     /score="135.6"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="YTEVFRDIRKTLIHMREFDIANSGTRFVVCGEPGYHWICAALAAI
                     FNGAQLVALPESMSEEEILASIDGLSIESALVADKYKASKAFASVKTFELSNFCEQISS
                     IDCDDDIVLSFMVSEKVIAFTSGSTAEAKFKAFEVPLQTSIAFTNRFRKLYSINKEDNW
                     LICHSFSHIVHFEYLLGGLYWGYNVTIATAFQILMKGASFNPSVLVTVPSVYEQIASQI
                     RNKFRLADDGENRLHALESRPISNENGDVIRYPLCEEAKLVLGDKLKMLLIGASPSSLE
                     LQTFLLQTGLPLYEGYGMTELNMISCNTPEKSKLSTVGPAWPGIEIKLDDDNCILARAT
                     PERARFYLNANQETNQSTFLDNGWINTQDLGRIDDGVLRIVGRKKDIIV"
     gene            11392..12210
                     /locus_tag="T451_RS0104115"
     CDS             11392..12210
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262:haloalkane dehalogenase (Score: 61.6; E-value:
                     1.2e-18)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /locus_tag="T451_RS0104115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_027148177.1"
                     /sec_met_domain="PF00561 (E-value: 2.8e-11, bitscore: 33.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.3e-17, bitscore:
                     54.6, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRSLIEINNLFIDVVMTEGHGDAIILLHGNSSSWRSFEFLFSSK
                     LAEKYQIIAINLPGHSNSNLPIDFYLSIPSLADLIVEVVHAISPKRYLLVGHSVGGHIF
                     SHSIQRMTDCNGLILISAPPVSMKTLSAAFKSDPCEGAIFTSELTSEKKELMVKALLGD
                     IADDSKAFNQLYESITGTDGLFRQKLGESLMQGKLLDEYEAIQDSKTPTALMWGGQDSF
                     IQPDFCAGITFNNPLGLGAYLFKNAGHSPHLSNSLMFEKILGDLAEKSMS"
     gene            12316..13686
                     /locus_tag="T451_RS0104120"
     CDS             12316..13686
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /GO_function="GO:0015288 - porin activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1076:RND efflux
                     system, outer membrane lipoprotein, (Score: 198.7; E-value:
                     2.4e-60)"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890606.1"
                     /locus_tag="T451_RS0104120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TolC family outer membrane protein"
                     /protein_id="WP_235202197.1"
                     /transl_table=11
                     /translation="MNTGLMPYQQLALIISIVCVMTTLTTKAFAEDKPQSLVEIYSKAL
                     AYDPTLASALTANKAIQEIIEQAKALYRPMVNFTAGANVSRSNIKFIGTEIPFNGGVQS
                     FEGYNYGVEVRQPLYRKQNLVQIDQAKTQVSQADKQLHLSQQELILRTTQTYFDVLITQ
                     DKVDLIAAQKESILSQLNQAKTNFEAGTVTITDVNEAQARYDLIVAQEIGAINEHQIAM
                     RTMQAITGETPQQLATVKADIKTNALEQGMEKWLDVALQNNLSIKVQEDLVQLSDQESE
                     RAQAGHLPTLDAVASYNDSYANGGFYGFGNELKNATIGVQLQIPLYEGGSTSSHARQAV
                     LGKQKARNDLDIARRKIELDTQRAYLNLSTSIAQVKAYEQALTSSQSQIDSTKIGYEVG
                     VRTIVDVLNAQQQFFGAKRDLLQARYSYLVSIIRLKFISGIVAETDLIDINQQLAVN"
     gene            13873..14895
                     /locus_tag="T451_RS0104125"
     CDS             13873..14895
                     /NRPS_PKS="Domain: PKS_ER (26-337). E-value: 1.4e-33.
                     Score: 108.1. Matches aSDomain:
                     nrpspksdomains_T451_RS0104125_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028:crotonyl-CoA reductase / alcohol dehydrogenase
                     (Score: 241.8; E-value: 1.7e-73)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890605.1"
                     /locus_tag="T451_RS0104125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_027148179.1"
                     /sec_met_domain="ADH_N (E-value: 9e-12, bitscore: 35.2,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 6.4e-28, bitscore:
                     87.7, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINSRSYRFARSGGASNLVRYDNTMLSPGPHEVLVRVSAASLNY
                     RDLLTLQDINSNHEGLIPLSDCAGTIVTVGTEVTRWKEGDRVSPNFFPDWHGGAFSLAY
                     LQRSLGGGQTNGVLSDFIVAEETSLVEITSHLTLSEAATLPCAGLTAWHALFERGALKA
                     GETVLVQGTGGVALFGLQLAVAQGAQVIVISSSDAKLERARTLGAWQTINYCTQPEWDK
                     VALELTDGKGVDHILELGGPETYDRSIAAIAAGGRIAQIGVLTGFASQPNILPLQFKNA
                     SINGICVGSVEHFKRLNDFLSAQQIHPVIDQVFDFDEVPAAYERMRSGVHFGKIVFDLA
                     "
     aSDomain        13951..14883
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_T451_RS0104125_PKS_ER.1"
                     /evalue="1.40E-33"
                     /label="T451_RS0104125_PKS_ER.1"
                     /locus_tag="T451_RS0104125"
                     /protein_end="337"
                     /protein_start="26"
                     /score="108.1"
                     /tool="antismash"
                     /translation="LSPGPHEVLVRVSAASLNYRDLLTLQDINSNHEGLIPLSDCAGTI
                     VTVGTEVTRWKEGDRVSPNFFPDWHGGAFSLAYLQRSLGGGQTNGVLSDFIVAEETSLV
                     EITSHLTLSEAATLPCAGLTAWHALFERGALKAGETVLVQGTGGVALFGLQLAVAQGAQ
                     VIVISSSDAKLERARTLGAWQTINYCTQPEWDKVALELTDGKGVDHILELGGPETYDRS
                     IAAIAAGGRIAQIGVLTGFASQPNILPLQFKNASINGICVGSVEHFKRLNDFLSAQQIH
                     PVIDQVFDFDEVPAAYERMRSGVHFGKIVF"
     CDS_motif       14293..14337
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_T451_RS0104125_0001"
                     /evalue="3.90E-05"
                     /label="PKSI-ER_m4"
                     /locus_tag="T451_RS0104125"
                     /protein_end="155"
                     /protein_start="140"
                     /score="16.9"
                     /tool="antismash"
                     /translation="AATLPCAGLTAWHAL"
     CDS_motif       14362..14421
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_T451_RS0104125_0002"
                     /evalue="4.00E-03"
                     /label="PKSI-ER_m5"
                     /locus_tag="T451_RS0104125"
                     /protein_end="183"
                     /protein_start="163"
                     /score="10.0"
                     /tool="antismash"
                     /translation="GETVLVQGTGGVALFGLQLA"
     gene            15164..16867
                     /locus_tag="T451_RS0104130"
     CDS             15164..16867
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018:isochorismate synthase (Score: 262.9; E-value:
                     1.2e-79)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890604.1"
                     /locus_tag="T451_RS0104130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="chorismate-binding protein"
                     /protein_id="WP_081813268.1"
                     /transl_table=11
                     /translation="MNTQKQYRRQLVNLLCGISAPKLMGAALEAILTSKELEDIEHRLQ
                     IFQMLTENTPQRQIAAKLKVGIATVTRGARAYRNGKFFELERNALQHIRSNPDINKSNM
                     DSQHIPKIQLPGKSNYIKLSEDDCFFELFRKIEKKYQHCFLFESLGEESGVSRNHIIGF
                     DPAQIVYAEDKHTLCITDTRTEKTEQYQCENSYYALREIVPQDIISRRYAGGLVGYLGY
                     DTINFFEPTLNLQTSESFEPFKFGVYLDGLVYDQMTGEIFYYYYNKNRIEEIKALLHQE
                     EEDFGETECTCLGDSMSREEHEQAVLKVKEYIRSGLIFQCEVGFKTRYKIIGDTTHIYS
                     KLREVNPSPHMFYLKFDQQKIIGASPELLFRLRDGEMETFPLAGTARRGADAIEDMQLA
                     RELLNDPKEIAEHNMLVDLHRNDLGRVARYGTVKVRSLMDIKRFSHVQHISSEIVGILN
                     EKEDMFSALASNFPAGTLSGAPKIEAIKIINELESDPRGPYGGAVGQFGFNGDCTFAIP
                     IRSVFINGEDGYVQTCGGNVYDSNPADEYQEIERKLSAMRSVLASFEKEQ"
     gene            16870..17514
                     /locus_tag="T451_RS0104135"
     CDS             16870..17514
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1249:carbamoyl phosphate synthase small subunit
                     (Score: 120.3; E-value: 1.5e-36)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890603.1"
                     /locus_tag="T451_RS0104135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="WP_027148181.1"
                     /transl_table=11
                     /translation="MRVLIIDNYDSFTYNLYQYVGEILSAKLGFAGFDVSVKRNDEITI
                     NQVIDFNPDRIIISPGPGTPSDKHYFGICSDVILELGKSTPLLGVCLGMQGIAYCFGGK
                     VVSAPVPMHGKISLVQHDERGLYRSMPQNFEVMRYHSLMVDAASLPDCLEITSVVADEV
                     TGQKQNLVDAALNGSVIMGVRHKKYPIEGIQYHPESFATEGGKKLLANFLL"
     gene            17605..18252
                     /locus_tag="T451_RS0104140"
     CDS             17605..18252
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GST_N_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1193:glutathione S-transferase (Score: 78.5; E-value:
                     8.8e-24)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890602.1"
                     /locus_tag="T451_RS0104140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glutathione S-transferase"
                     /protein_id="WP_027148182.1"
                     /sec_met_domain="GST_N_3 (E-value: 5.4e-19, bitscore: 58.8,
                     seeds: 52, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINLCGLAVSNYYSKIKLVLLEKGIEFKEEIVTPSQDAALLKRSP
                     LGKIPFIETEQGNLSESQVILEYLEEVYPEQPLYPADNHQQAKCRELIQHLELNIELNA
                     RRLYTESFFGGTVSVETKDQVKDLLERGLKGLARLAKFSPYIAGNSYTAADCVAWSHFF
                     AVRLATQKIYGVDMVAEYLPGVNEYMLLIETRPCVQKVIADRAAAMTQFLNG"
     gene            complement(18478..18903)
                     /locus_tag="T451_RS0104145"
     CDS             complement(18478..18903)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF027570.2"
                     /locus_tag="T451_RS0104145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4902 domain-containing protein"
                     /protein_id="WP_036304852.1"
                     /transl_table=11
                     /translation="MHPENITGVDIITISPDGYIRLTFEEFQQVSLVHLMSGLDEGDNL
                     PLVQEGATFAEFTGYTDWVSATSPAISIGWDWMNQFSQLGGVYYKRVSKSRSNLMLVDA
                     KQRDLGPDKTAALIETVVDGIVWQIVVQSYISTRYAS"
     gene            19074..19784
                     /locus_tag="T451_RS0104150"
     CDS             19074..19784
                     /codon_start=1
                     /gene_functions="regulatory (smcogs)
                     SMCOG1197:autoinducer-binding transcriptional regulator,
                     (Score: 270; E-value: 2.6e-82)"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446790.1"
                     /locus_tag="T451_RS0104150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="autoinducer binding domain-containing protein"
                     /protein_id="WP_006890625.1"
                     /transl_table=11
                     /translation="MKAWQETQLQALQSIQCAHQLFQTILSFSKELGFDHCAYGLRMPL
                     PLTNPKTLMFNNYPTAWQAQYQAKNYLAIDPTVQHAMRSLLPIIWTDDLFSPASDLWEE
                     ACSFGLRVGWAQSSRDFNGFVGMLTLARSNEPITEIELDANKFKMAWLTQVAHLGMSRC
                     LTPKLMQEVNVTLSNREIEVLRWTADGKTSSEISCILNIAERTVNFHINNVVTKLNAAN
                     KTSATIKAAMLGLL"
ORIGIN
        1 atgaagttta tgttaagtaa gagtatggaa gtctgtgcat tttttgatgt agatgaaacc
       61 atttggaatg aaaaaagtgt aataacattt tatcaatttt acttggatgt tactaaaggt
      121 gataaagctg attttttttg ggaacagttc aataaaaatg taaaaaatac tattagtgca
      181 ggtatatcaa gagaagagct aaatgcatgg ttctatcttg aacactttag tggagtgtca
      241 gtatcgcact tacgcgaaat tgcagaacaa tggagtataa aaaagtttaa tcagccaagc
      301 ttttggcaag ataaagttat tgaaagaatc gaatatcaca aaaataaagg gcacgcaatt
      361 gtattggtta gtggatcctt cagagaagtt ctagcgcctc ttgcaaagcg tttgggtgta
      421 acagatattc tttgctcgcc cttggaagag aaggatggat gctatacagg aaatatgctt
      481 ggctctccta tgattggata tggaaaagca aatgcagttt cagattatct gcgtgagcat
      541 aaaattgatc cagttcattg ctacggatat ggagatgata ctaccgatat cccttttatt
      601 ggcacagtgg gaaactctta catcgttata aataataacc acgatgaact aagtgtctta
      661 gctgagatta tgggctatca aacactgaac gtttaaatat atctatttta attttgaaat
      721 tatgactaat aggagtcacg taaaatgatg agtttagaaa atctacttgg agatgataat
      781 gcagcaagta ttaaaagaca gctcgaccat gccccattta gacgtttgtc tgaaaaaaat
      841 gagacttata ttaagccaga gtggctttgc caagcttttc atatcaatcg actaacaggt
      901 gagcctaagt taatacttgg cgatcaagtc ggcggttatt atatgcactc gatcgaatcc
      961 attaagccaa gcgttgacaa aatgcttaat cagaacatta aacgagtata tttaggaacc
     1021 gatgctgtta ttggagaaga aggtagctac cttgccaagc taaatgattt tgcaaacata
     1081 ttagtgtcct tgcgtacaat tgtagggcct gatgtagaaa ttattgtaga tcctgctgga
     1141 ctttgtttac gtaaggattt gcgctgggga gttactgccg agggtggaga catcaacgcc
     1201 caagaaacgc ttgctttgtt agctcaggcc gcagtcacat ttgaagaggc tggagcaaat
     1261 gcgcttttga ctattggcag atcaaattgc gaagtggctg ctgttaagca agcgttgcag
     1321 tctaaaaata aggacatgag agttctttct ttttctacca attcagaaac tacctcggct
     1381 tattttgaag taactcaaca tgacattctt agatcaagaa cggggcaaaa aatatttgtt
     1441 ggtaatattg aagaaatgtt ggttcgggca atttgtgatt ttggcgaagg cagtgatgtt
     1501 atcgtacaaa aaccggtcga gtcgtttcac ttacccgcta ttttacgact actatcggaa
     1561 ggcctaattt catttgagtc cttaatggat aactcagaaa ccatagatat attattgaat
     1621 aataatcctc atatccgtcc tgcttttaat gccggtgtag aactgcttaa atctaaaaag
     1681 cgcattctta aaacaggtac gtacgaggtt tccggtacct atagcactat tcagctgata
     1741 attaatcgtt atagcgaaca attaggctgg tctatgcttg acgaaatact acttaacgca
     1801 gccagcgcag caggtaaatc gttggatata atgatttcgc gtaatgcgac atggtacctg
     1861 gaaaagcgcg gactatacag tatcaaggaa tcttaactaa gttatttgga caacttaaat
     1921 taggataaag gaaagttcat gtctaaaaaa atgatactcg taggcgatca atttaaagaa
     1981 tttgctaatg aagagagcgt tctgacatta acacacttcc gatcggcata ccaacaagat
     2041 aaaaacatta actcaagaat agttgtggga cagggtttga gtcacgatga ggtcaatcac
     2101 tggcgtaatt cagggttgaa tgtggaagct ccatccaacc ctgcatcgct aaaagtgaca
     2161 cacaagcatc atttagatca cgtaatgatt agcgaaccca aaaaaggtga agcatttaac
     2221 taccagatgg atttaatgct aaatgatgaa acagatcgtc tccttgacca tatcaccagt
     2281 ttgcatgtta gcggcatggt tttgatagag gcggctagac aggcttctat agcatgtaca
     2341 gaaatcgagt acgattttct taatagggaa aaaccatccg gttatacatg gacaaacatg
     2401 aatgttaggt tttcacaatt catattccct gttcctacaa gaattgaatt ggaaatagtt
     2461 gaacgggaga acaacgaaaa aagattcacc ttatcatcaa aagtaaattt tattcaagga
     2521 gataatgcag tttgcgatat ggaaatgatg tttgatgtat tacctttaaa gcttattaat
     2581 cagattgaaa ggcgttcttc ggcaaagata attaggaatt taactttaac ggattaagat
     2641 gaaaatagac actcatattg tgaaagtttt aattgtcgga gccggcccga cgggcttgac
     2701 tttagcggca tgcttggaaa agtttggtat ttcccctgat attgtcgatt ttaaaaattg
     2761 ccctaatgat cagagcaagg cgctggcaat caatccggta gcccaagctc aattaggaat
     2821 tatttttgga aaaaacaatg ttggcaaaaa ttcaaatata gtcacaaagc taaaggtcat
     2881 atttaacacc gataaacgat taacgactat cgatttaaag catcttggat ggccttgcaa
     2941 ctctatgtta gtgcaaagtc aatatgatac cgaaaaggac cttattgaac ttgctaaaaa
     3001 taatagcgtc aatgtgcgat gggaaactcg ggtcgtagag gtcatcgaac atgatgatgc
     3061 ggttcaagta gtatttgaag gccccgacaa tcaaaccatc aaacaacaat atgattatgt
     3121 aattggctgc gaaggcaagc aaagcttggt gcgcgaagct atcggtgcga ctatgacacc
     3181 gctgccttat tcaatgtact tggcgcttgc cgatttcaag ttagatatcg atttgagcga
     3241 agacagtgca tattattttg tatatgagga cacttttttt gtcttcgtgc cattaggtaa
     3301 aagtatttgg cgtattgttg taaagcataa tggcgccccc aagagagggg aatacattaa
     3361 tcttgtaacc gaccccgtat tggaaaaatt tggccgcaat attttttgcg gcgaatgcct
     3421 gtggttttcg caagcgccac tttatgtaag ttacgccaat aaattacaaa gcaaacgctt
     3481 gtttattgca ggcgactctg cccacctgtt cagtcctatt agtggaacag gtatgaacac
     3541 gggtatagtc gatgccgtaa acctggcttg gaagattgct tttactataa aaggaaatgc
     3601 ctgcggcaat acccttattc agtcatatca ggaagaaaga atccctgcta ttaaagaaaa
     3661 tgctatggcg acggaccgct taacaaaatt gatatccagg caagaagaac ataccgatca
     3721 atttatgccg aaaatgtcta atagggctta catacggaat ttgtttccct tgaatgtttc
     3781 tggattcggt ttttcctacc cgtcaagccc ggttttattt ggcaagaccg atgatcctag
     3841 tattggacgt atggattcaa aactgactaa actgttgatg tcttcatctg aatttattcc
     3901 agactttagt ttatacatta atgtattgca ttttttggat caagacctta ataaaacagt
     3961 tctaccgtgt ccaaaacaaa caagactcaa aggagtgagg gatgttgtcg ttcagtggat
     4021 taataacgac gaagttgtag attcttcttg tatgcaagtg caacaatcac caaatatcat
     4081 aaatattgcg ttacttaaaa aagattggca ggagctacgc cagttaggcg atcttcaagt
     4141 attaaggccg gatggaattg tagtatttcg cggttcaatt aaagagcatg gcgaggtagt
     4201 tataaatttg tcggaatatt taaatttaca ataagggata tttagtaatg aacaataaaa
     4261 atattttatc taatgccatt cgtataggtt taggatgtat gggcatgagc gaattttacg
     4321 gtgaaacaga tgatgacaat tcccttgata tattacatgc ggcttatgac ttaggttatc
     4381 gtcatttcga cacagcagat atgtatggta aaggtcacaa cgaacgtcta attggtactt
     4441 ttgttaaaga gctcggtagt cgaggcaagg atattttaat tgcaacaaaa gttggtatta
     4501 agcgagatgt gaatggacct ggcactctag taatagattc aacgccggaa tatattcttc
     4561 aggcctgcga gcaatccctg aatcgacttg gagtagagca aatcgatttg tattatcttc
     4621 atcgtcgtaa ccccgatgtt cctattgaag aaacaatggg agcaatgaag cagttactag
     4681 atgaaggaaa aattgccgcg gttggtttga gtgaagtttc tacagaaacg ctcaaacaag
     4741 caaatagtat agtgaaagtt agcgcactac aaagcgaata cagtttatgg tcacgcgata
     4801 ttgaagacag tattttaccc gcatgcaacg aactcaatat caacttagtg gcctattctc
     4861 cgatagggag aggtttttta tcaggcacgc tcaaaaaaga gcaagttcat aatgagggag
     4921 atttgcgtgg caaattaccc cgatttcaac aagaatcctt cgaaaacaat caaaaattat
     4981 tagatgcagt gaaacatgtt gccgacgaaa aaaattgctc gttggctcaa gtcgctttgt
     5041 catggttgtt gggacaaaat ccattggtgg cggttattcc tggcgctcgc aaattaaatc
     5101 atttaataga taattttgcc agccaggata ttcatctctc aaaagaacaa atctcatatt
     5161 tatcggaagc gttcaagccc gataatgtgt ctggttatcg ctaccctgaa cctcttttaa
     5221 aaactaccaa tacttaatat ataaaattaa tgtatgccaa ggcagaaaac aaatggttgg
     5281 tggcggcatc tgtaactact ggcgctgtta tggctgctgt ggacacttct attctcaata
     5341 ttgcaacccc tcatttacgc ggggtttttt cagccacaac agcagaaatc agttggatca
     5401 gcactggata tttgttagct gtggtaattg gtatgccgct tacaggctgg ttatgcagtc
     5461 aatttgatcg taaaaatgta tgccttggag gattggctgt ttttattttt gcgtcgattt
     5521 tatgtggttt cgcaacggag cttggtttgc ttgtcgctgc acgtatttta cagggattgg
     5581 gagcgggcgt gttattgcca gtggagcaag tcattcttcg gcaagctttt cctccaaaag
     5641 aacatggctt ggctatggga ttatatggtg tgacggttat gatcggtcca acaatagcgc
     5701 cattattagg cggcttaatt attgataatt atcactggtc gttgattttt tttgtcaatg
     5761 tccctgttgg tctggcaggt gtttttatgg tgcatcgatt tgttcaaaac aaaccggcgc
     5821 agggtgaccc agttgtttcc attaagccag actggatagg aatactttta ttggctatgg
     5881 cattgttttt tttgttatgg ctattagaaa gaggcgagag gttagattgg tttgaaacac
     5941 aatccaatgt ctggttatta gttgtttcaa tttcctcatt taccctgttt tgtgcgcacg
     6001 aattaataac gaaaaaacct gccgtagatt tgcgtatttt gaataatcgg gctttttctt
     6061 cagctgtatt tatgagcttc gttcttggtt ttgtggtttc tgcaaccctt tttgttttac
     6121 cgatttacat gcaagatgtc cttggttttt ctgctactca tgcaggcgag gctcttgtcc
     6181 ctcgtgcttt ggttatgatg attgcatttc cgcttatagg tcgcttgtat aaccatgttt
     6241 cgacaaagct attaatggct atcgggctag cgcttggttt tttcagcgct atattgatga
     6301 gcaagtttac taaagtaacc ggcatacacg atattatctt gcctcaaata ctacaaggag
     6361 tcgcagtatc atgcatttta acgccgttat cgacagtggc tttacttcac gttgctgccg
     6421 accgcttagc cgcagcagcc ggtttaaatg cattttccag acaactgggc agtagtattg
     6481 gcattgccat ttgttcaagc ttaatcagtc actttgagtt agtcgtcaga ggggctctta
     6541 tccatcatat caattgggct aatccagttc tccgcgacag atttacgggg gtaattagat
     6601 atttttatac aaagagtatt aatgactata caacagcgat gcaccaaggg ttttcacagc
     6661 taaatagcaa tgtgaccaag caagtagtgg ttatttccta tcaaaaaact tttgaatggc
     6721 tggccgttgc ctttatcgtc atgttcattt gtttgttttt tatgaaaaca aaacgtcatg
     6781 agtatatata gattctttcg catgagggaa aagagagatt ctcctacctg catactcaac
     6841 ccgttgctta aagaagctct agtatagcta tgattaataa attgaaaaca gattaccgga
     6901 tctgggcagg gagtatagtt attattttct gctctttgtt agctggctgg cagagtataa
     6961 atttcgacaa agagtccact gataatgcaa ttgtcaggtg cgatgtggtc gatgtgacat
     7021 ctcaagtcag cggcataata caatcagttc attttcacga taaccagctt gtgaatgtgg
     7081 acgatgactt aattaatata gatcctgatt tatatcgtgc ccgcttagat agagcggaag
     7141 ctcgactcaa tatcgccgaa ctttcttaca gtgctgcgct taaagatgag ttgctttcag
     7201 aaattaatgc caaaagcgac ttcgaacgct caaaagattc tttagatgcg gctttagcaa
     7261 acttggaggc aataaagagt caaatggatg aagcaaaagc atcctctcgt tcagccaaag
     7321 tagacttaaa attggcggaa gcaaattatc aaagggttct cagtattttt caaaaaggga
     7381 tgacttcaaa agcgagtttc gataacgcag aaagtaatat ggattcgaaa aaagcacttc
     7441 tcgctgcctc tcaagctaat gttgaagcac aagaaaatca gtttggcgca gaaaaggcgc
     7501 gggtggccga aattagaaaa cgttctaacg tttatgggca atcagaggga agtcttatat
     7561 caaaagctca agctaacacc gaaatttcca gtggtaatgt aaaggtggcc cgtgccgagc
     7621 gtgacttggc agcgctggat tattccagaa caaaaataaa ggcttttcgg tctggaatta
     7681 ttaccaaccg gaaagtagct cttggtcatt atgttgaagc caaccagccg attggcagca
     7741 ttgtaacatg tacggaaaaa gcatggattg aagcgaatta taaagaaacg caagtaggaa
     7801 ggatgaaacc tgggcagcct gttaagttta ctgtagatgc ctaccccgat gtcgaatttt
     7861 tgggtgaagt tgaaagtata tccggtggtt ccggtgcaat tttcagttta ttgcctcctg
     7921 aaaatgcaac gggaaacttc actaaggtgg ttcaaagaat tcctgtcctc ataaaaatag
     7981 accaagagca aaatgtgaag atgcgcgtgg gaatgtccgt cgttgcaact gtcataaccg
     8041 aataacttct ataggatata tgtttgtgga aattgaaaaa aatcttttaa atgcacttag
     8101 acaatcaatg cctaagccaa tagcagaccg ggttattgaa gcggattcaa atctttcatt
     8161 tgatttaggc attgattctc taggcttgat gacactatct gtaaacattg aaaacgctta
     8221 tgcaataaat ttagttgatc atgtggaagc attaatgacg gtcaaatcag tttctgaact
     8281 tcaaacgctt attcacagcg ttttatagag attaatatgg aaaaaattcg tgaattgggt
     8341 tttggtgagc tgagaatgca cttactgcat tccctagggg atggtacaac tcagggggcc
     8401 ttgcatgtca gaatcgaagg ggagcttgat gataaatatt tagaatcagt cgttgaaact
     8461 ctattaatta aattaccggt attaagagcg acaattcaaa aaattgatgg gcggtattat
     8521 ttttgtttag ctgacagaaa tcatcatagg aatggttttt tagatattac cgtagataag
     8581 aacgccccaa acctttggca agctaggctt gaggcggaaa ataatttgct tctcaattct
     8641 gaagagcagc tttggaggct ggtagcggtg aattatcagt cagtaaattg ttgggatctg
     8701 atattggtca tgcatcatgc gctcatggat gcagcaggta gtgaattttt gcttgatcaa
     8761 atcttgggct gtcttggtaa tttatatgaa attggatcgc tagaaagcac agataatgtt
     8821 tttccttctc tagaagagga tactgtaaca gcttgcgaat gggaaaaata tgttggtatt
     8881 caaagtaata taaaaattaa attggcatca ttaaatctgc aaaagcatct gagttcagtt
     8941 ggtctcaacg agcggaatac aaagtctcaa tacttccagg tcaaccaaga cctggttgcg
     9001 caactagagg atttctgcga gattaatcag atttccctca attcattatt ttccgccgcc
     9061 ctattgtcat caatcgcctt atgtacgcca gatagaaaat catttgcact cttttctgcc
     9121 gtttcattgc gcccattgtg tcagaacgta aaagaaagaa gtttggggtg ttacctaaat
     9181 gttatgccca cggttcattc attctcagat gacagtgttt ccagcattat atcccttagc
     9241 cgtgaacata aaattgaagt tcagcgagcg tttcttaata ctggacgttg gataccgaga
     9301 aattatgatg ttgcgatagt cgagaatatg atttcaactc tcgctaattc gaatacattt
     9361 ggcaatgata ttggtatcac ctatgcagag tcaggattag ctccacagta cggtaagctt
     9421 aaagtcaagc atcaatatgt taccgcaaga cgcaatatag gcaatgtagc tttaatcctc
     9481 catggtttaa agtccgatcg agatatatat ttttctttaa gtcatgttga gcctttgcaa
     9541 tcaagagaat gggctatttt ggtaaaaaac aaattccttg acttgctgct tatgattagt
     9601 aagtcctcat ttgataccgt taaaatttaa agacatatat tatggaacaa tacactattt
     9661 tagatttaca taaacgcata gcgacaaact cgactaactg cttaacattt ttcaataagc
     9721 agggcgaact tatttcaaaa ccttataccg aggtttttcg cgacattcgc aaaacgctta
     9781 tccatatgcg cgaatttgat attgcgaatt cagggactag atttgtagtc tgcggcgaac
     9841 ctggttatca ctggatttgc gctgctttag ctgccatttt taatggcgca cagttagtcg
     9901 ctttgcccga gagtatgtca gaagaagaaa tacttgcctc tattgatgga ttaagtattg
     9961 aatcagcatt ggtagcagat aaatataagg ccagtaaagc atttgcttcc gtaaaaacat
    10021 tcgaattgtc taatttctgc gagcagataa gctctattga ttgtgatgac gatatagtac
    10081 ttagttttat ggtatctgaa aaagtaatag catttacttc cggctctact gccgaagcta
    10141 aattcaaggc atttgaggtg ccattacaga cctccattgc ttttacaaac agattccgaa
    10201 aattatattc aattaataaa gaagataatt ggctaatttg ccactcattt tctcatattg
    10261 tacatttcga gtatttatta ggagggcttt attggggata caatgtaact attgctacgg
    10321 cttttcagat actaatgaag ggggcttctt ttaatccttc cgtattagtt actgttccga
    10381 gtgtttacga gcagattgcc agccaaattc gcaataaatt ccgcttagcc gacgacgggg
    10441 aaaacagact acatgctttg gaatccaggc caatatcaaa tgaaaatggt gatgttatta
    10501 ggtatcctct ttgcgaggaa gcaaagctcg ttttaggcga caaattgaag atgctattga
    10561 taggagcgtc tccaagttct ttggagttgc aaaccttctt gctgcaaacc ggattgccat
    10621 tgtatgaagg atatggcatg acagagctta acatgataag ttgcaatacg cctgaaaaat
    10681 caaaactttc aactgttggg cctgcttggc ccggtataga aataaagtta gatgatgaca
    10741 actgcatact tgctcgtgct acaccggaga gagctcgttt ttatttaaat gccaatcaag
    10801 aaaccaatca atcaacattt ttagataacg gttggattaa tactcaggat ctgggcagaa
    10861 tcgacgatgg cgtgttgcgt attgtcggta gaaaaaaaga cattatcgtt aatgccggcg
    10921 gtaaaaacat tagcccttct tcgatagaaa caaaattaac ttctattcca actgtcaatc
    10981 atgcagtagt ttatggcgat atgaagcctt atttagtcgc tgtcctttcc tcgacagagg
    11041 aagaccgttt tttattagaa gatattcagt gttctttgga taggatcaat acgtcgttac
    11101 cactgcatga aaggattatg ggctttattt tatctgagga acctttcact gtagagagtg
    11161 gaacattaac gaggttaggt aaagttagaa ggacaaaagt cattgaacga tatcaggatg
    11221 aattagaaaa actttatgcc taactattca gttataaaaa gcgaattaaa cggacaagta
    11281 ctgatttaaa agtgcataac aaacttggct taatacgtcg cgtaaatcag tccagtccgc
    11341 actctattaa tcatggttag cattaacctt tatactttat aaggttttat tatgaaaaga
    11401 tcacttatag aaattaataa tttatttatt gatgtggtga tgactgaagg ccatggcgat
    11461 gcaattattt tattacacgg taattccagt tcatggcgca gttttgaatt tttattctcg
    11521 tcaaaattag cggaaaagta ccagataata gctattaatt tacctggaca tagtaactca
    11581 aatttgccaa ttgactttta tttgtctatt ccaagtcttg ctgatcttat tgttgaagta
    11641 gttcatgcca tttcgcccaa gcgttacttg ttggttggtc atagtgttgg ggggcacatc
    11701 ttcagccatt ccattcagcg tatgacggac tgtaatggat tgatattaat tagcgccccg
    11761 cctgtttcca tgaaaacttt atctgctgcc ttcaaaagcg atccatgcga aggtgcgatt
    11821 tttacctctg aattaacatc agagaaaaaa gaactcatgg tgaaggctct gctgggcgat
    11881 attgctgacg atagcaaagc attcaatcaa ttatatgaat ctatcacggg aacggatggt
    11941 ctttttaggc aaaaattggg tgagagtctg atgcaaggaa aattgctaga tgaatacgaa
    12001 gccattcagg attcgaaaac accaactgct ttaatgtggg gtgggcagga ttcttttatt
    12061 caacctgatt tttgcgcagg cataacattt aacaatcccc ttggtttagg cgcctattta
    12121 ttcaaaaatg caggacacag cccacaccta tctaactcat tgatgtttga gaaaatatta
    12181 ggcgatcttg cagagaaaag catgtcttga aacagtgggg taataaaata attgcattcc
    12241 gatatcgtaa ttgatgtgcg aggcgccttg agtttctgat ttgcacagtt gagtttttta
    12301 taaaatttat atcacatgaa cacaggttta atgccgtatc aacagttagc tcttattatt
    12361 tcgattgttt gtgtaatgac tacattgacg actaaagcat tcgctgaaga caaaccacaa
    12421 tctctggtgg agatctatag taaagcgctg gcgtatgatc caacgctggc atctgcgcta
    12481 acggctaata aggcaataca agaaattatc gaacaagcca aggcacttta tcgaccgatg
    12541 gttaatttta cagctggcgc aaacgtctct cgttctaata ttaaattcat cggaacggaa
    12601 atcccgttta atggtggcgt acaatctttt gagggctaca attatggggt cgaggtacgt
    12661 cagcccctat atcgcaaaca aaaccttgtg cagatagatc aagccaaaac gcaagtaagc
    12721 caagcagata aacaattaca cctaagccag caagagctta tcttacgcac tacacaaact
    12781 tattttgatg tgctaattac gcaagataaa gtggatttaa ttgccgcaca aaaagaatct
    12841 attttaagtc agttaaatca ggctaaaacg aatttcgagg cgggcaccgt aacgatcacc
    12901 gatgtgaatg aagcgcaagc acgctacgat ttgattgtgg cgcaagaaat aggcgccatt
    12961 aacgaacatc aaatcgccat gcgtacgatg caggctatta cgggagaaac gccacaacaa
    13021 ttagcaacgg taaaagccga cattaaaaca aatgccttag aacaaggcat ggaaaaatgg
    13081 ctagacgtcg cattacaaaa taatttgagc atcaaagttc aagaagatct agttcagttg
    13141 tcagatcagg agtctgaacg cgcacaggca ggacatttgc ccactttaga tgcggtggct
    13201 agctataacg atagctatgc aaatggtggt ttttatggtt ttggcaatga acttaaaaat
    13261 gccacgattg gagtacagtt acagattccc ttgtatgaag gcggttccac tagttcgcac
    13321 gccaggcaag cggtgctcgg taaacaaaaa gcacgaaatg atttagatat tgcacgtcgc
    13381 aaaattgagc tggatacaca acgtgcttat ttgaacttga gcacaagtat cgctcaggta
    13441 aaagcctatg aacaagcgtt aacaagtagt caaagccaga ttgactcaac aaaaataggc
    13501 tatgaagttg gtgtgcgcac catcgttgat gtgctaaatg cacaacagca gttctttgga
    13561 gccaaacgcg atttgcttca agctcgctac agttatttgg tgagcatcat ccgcttaaag
    13621 tttatatccg gcattgtagc ggagacagac ttaattgata ttaatcaaca attggcagtt
    13681 aattaactga aggtaaattg acagatttac tgttgaactt acaaagttta acgatcacgg
    13741 aaatcattga gagtcgccct atcagtgaat agccccaggc tctgaagaga agaaaacacc
    13801 gctttgcacg aagccgcctc agtagcacct tcgtgattgc gtcataaagc acacttaacc
    13861 ttaggcatga acatgaccat aaattcccga agttaccgtt ttgccagatc aggtggagca
    13921 agcaatcttg tccgctatga taatactatg ctcagtcccg gccctcacga ggtactggtt
    13981 cgggttagcg cagccagcct caactaccga gacctgctta ccctgcagga cataaactca
    14041 aatcacgaag ggctgattcc gctgtctgat tgtgccggca ccatcgtgac cgtgggtact
    14101 gaggtgacgc gttggaaaga aggcgaccgt gttagcccca attttttccc cgattggcac
    14161 ggcggtgctt tctcgctcgc ataccttcaa aggtccctag gtggcggtca gaccaatggt
    14221 gtgctgagtg acttcatcgt tgccgaagaa acatctttgg tcgaaatcac atcgcacctg
    14281 acgctgtccg aagcggctac tttaccctgt gcaggcttga cggcttggca tgcactattc
    14341 gagcgtggtg cgttgaaagc cggtgaaact gttctcgttc agggcactgg aggagtcgcc
    14401 ttgtttggct tacagttggc cgtagcacag ggcgcgcaag tgatagtgat ctcatcgtca
    14461 gacgccaagc tggaacgagc gcgaacactc ggtgcttggc agaccatcaa ctactgcact
    14521 caacccgagt gggacaaagt cgcacttgaa ctaaccgatg gcaagggtgt ggatcacatt
    14581 cttgagctcg gcgggcctga aacctatgac cgctccatcg ctgcgatcgc tgccggtgga
    14641 cgaatcgctc aaattggtgt gctgaccggc ttcgcttcgc agcctaatat tctgccgcta
    14701 cagttcaaga acgccagtat taacggcatc tgcgtgggct cagtcgagca ctttaaacgg
    14761 ctcaatgact ttctctccgc ccaacagatc caccccgtga tcgaccaggt ctttgacttc
    14821 gatgaggtgc cggcggccta tgaacgcatg aggtccggcg tacatttcgg caagattgtg
    14881 ttcgatcttg cctgagctga cgtacggacg ccagatggct tatgccaatc cacatgacaa
    14941 cactgtttca gctcacctga ctcactgtca gagccgacga attaaacaac atttactttt
    15001 gccgctgcga gtagtcgacg gcaaattgga acaaacgttt cggggtggca tcccggcttt
    15061 cgagctgcga atgctgaaaa actcaaaaaa gttgcttcgc tgccccttgt tttgcaaact
    15121 ctctatttgc aatagcttat tgcgcgtact acaatactag tacatgaata cacagaaaca
    15181 atatcgacgg caattagtga atttgctttg cggcatatcc gccccgaagc ttatgggggc
    15241 tgcattagaa gctattttga cgtccaaaga actggaagac atcgagcatc gcctgcaaat
    15301 ttttcagatg cttacagaaa acacccctca gcgacaaatt gccgccaaat taaaagttgg
    15361 tattgcaact gttactcgag gagctagagc ataccgtaac ggcaaatttt ttgaactgga
    15421 acggaatgcc ttacaacata ttcgcagcaa tcctgatata aataaatcta atatggacag
    15481 ccaacacatc cccaaaatac aactacccgg aaaatccaac tatataaagc tttccgagga
    15541 cgattgtttt ttcgaattat ttagaaaaat tgaaaaaaaa tatcaacact gttttttatt
    15601 cgaatccctt ggcgaggaga gcggggtttc ccgtaatcat attataggat ttgaccccgc
    15661 tcaaattgta tacgcagaag acaagcatac gctctgtatt accgacaccc gcacagaaaa
    15721 aactgaacaa tatcagtgtg aaaattctta ttatgcgctt agagaaattg ttcctcagga
    15781 catcatatcg cgccggtatg cgggcggatt agttggctat ttagggtacg acactataaa
    15841 tttttttgag ccgacactta atcttcaaac aagtgaatcg ttcgagcctt ttaaatttgg
    15901 agtatatctt gatgggctag tttacgatca gatgacaggt gaaatatttt actattacta
    15961 taataaaaac cgtattgaag aaataaaggc cttactccat caagaagaag aggattttgg
    16021 agaaaccgaa tgcacttgcc taggagactc gatgtctcgt gaagaacatg aacaagcagt
    16081 gttaaaagtt aaagaatata ttcgatcagg gttaattttt caatgtgagg taggcttcaa
    16141 aactcgctac aagatcattg gcgatacaac ccatatttat tccaaattac gggaagtaaa
    16201 cccttctccg cacatgtttt accttaagtt tgatcagcaa aaaattattg gagcatcacc
    16261 agaacttttg tttcgtttac gtgatggcga aatggaaact tttccgcttg caggcaccgc
    16321 aagacgaggt gcggatgcca ttgaagacat gcaactggca agggaattat taaacgaccc
    16381 taaagaaata gcagagcaca atatgttagt ggatttgcac cgtaacgacc taggtcgggt
    16441 agcgcgctat ggtactgtca aagtccgtag tttaatggat ataaaacgct tctctcatgt
    16501 acagcatatt tcttcagaaa ttgttggcat tctgaatgaa aaggaagata tgttttctgc
    16561 cctggcttca aatttcccag ccggaactct atccggggca cctaaaattg aagccattaa
    16621 aattataaac gagctagaat ccgatcctcg cggtccgtat ggtggcgccg ttggccaatt
    16681 tggttttaat ggcgattgca cgtttgctat tcccattcgc tcggtgttta tcaatggtga
    16741 agacggctat gtgcaaacgt gtggaggcaa tgtctatgac tctaacccgg ctgacgagta
    16801 tcaggaaatt gaacgcaagc tttccgcaat gcgcagtgtt cttgccagct ttgaaaagga
    16861 acaataatca tgcgcgtgtt aattatagat aattacgatt cttttactta taacctgtac
    16921 cagtacgttg gcgaaattct atcggcaaaa ctggggttcg ctggttttga tgtgagtgtc
    16981 aaacgtaacg atgagataac tatcaatcaa gttattgatt ttaaccctga ccgtatcatt
    17041 atctcaccgg ggccaggaac ccctagcgat aaacattatt ttgggatctg tagcgatgtc
    17101 atattggaac taggtaaatc aactccattg ctgggggttt gccttggcat gcaaggtatt
    17161 gcgtattgtt ttggtggaaa agttgtgagt gctccagtac ccatgcatgg aaaaatatca
    17221 ctggtgcaac acgacgaacg tggattgtat cgctccatgc cacaaaactt tgaagtaatg
    17281 cgttaccact cgctaatggt agatgcggct agtttgcccg actgcctgga aattacatca
    17341 gttgtcgcag atgaagtaac cgggcaaaaa caaaacttag tcgacgccgc tttaaatggc
    17401 agtgtaatta tgggagttcg ccacaagaaa tatcccatcg aaggtattca atatcatcct
    17461 gaatctttcg caactgaagg tggtaaaaag ttattagcaa actttcttct ataaaacata
    17521 tatttgggaa atatttacaa cttttagtaa ccaccgaaaa aagcagtaaa tttcagcatc
    17581 ctttttgcta ttacggaaat cattatgatt aatttatgcg gcttagcagt cagtaattat
    17641 tacagtaaaa taaaactggt tctacttgaa aaaggcattg aatttaaaga ggaaatagtg
    17701 actccttctc aagatgcggc tctgctaaaa cgctctccat tgggcaaaat accctttatt
    17761 gaaaccgaac aaggcaattt aagtgaatcg caagtaattc tcgaatattt ggaagaggta
    17821 tatcctgagc agccgcttta tcccgcagat aaccatcaac aagccaagtg tagagaatta
    17881 attcagcatt tggagctcaa tattgaattg aatgctcgcc gattgtatac agaatcattt
    17941 tttggcggca ctgtctctgt agaaaccaag gatcaagtta aagatctatt agagcgcggc
    18001 ttaaaaggct tagcacgttt agcaaaattc tccccgtata tagccggcaa ttcttacact
    18061 gcggccgatt gtgttgcctg gtctcatttt ttcgcggtga ggttagcaac gcaaaaaatt
    18121 tatggggttg atatggttgc tgaatattta cccggtgtga acgagtatat gttgcttatt
    18181 gaaacacgtc cttgtgtaca aaaagttatc gccgacaggg cggcagcaat gacacagttt
    18241 ttaaatggtt agtctgaaaa acggccgaat aggtcctgtt ccgtataatt aaatgtttga
    18301 agttgccgac caaatcgcgc cacaaatact ttgtggcggt agcaggcgag tttcgagtat
    18361 agccttgtga gagcccttct ggtctacctg taacaacgta cgtctctccg aaaaaatttt
    18421 tcttagggcg catttgaatg acttttgtcg ctagaaacaa ctgtcaatat ttacaggtta
    18481 tgacgcgtat cgtgtgctta tatagctttg aactacgatt tgccagacaa tcccatcaac
    18541 tactgtttca atcagggcgg cagtcttgtc aggtcctagg tcgcgttgtt tcgcgtcaac
    18601 cagcatgaga ttacttctcg atttactcac ccttttatag tagactcccc ccaattgaga
    18661 aaactgattc atccagtccc agccaatgct aatggctgga ctggtggcac tgacccagtc
    18721 agtgtagcct gtaaactcag caaaggttgc tccttcctgt accaaaggaa gattatcccc
    18781 ctcatcaagg ccagacatta aatgtacaag tgaaacttgc tgaaactctt caaaagttag
    18841 gcgtatatag ccatcagggg aaatagtgat gatatctact ccagttatat tttcaggatg
    18901 catgtttttc tcaactaagc taagaacaag ataaaacaaa acacactaca caaagtcaat
    18961 acttgtcgat agtgtcaaat ctgacagact tacgcttttg ccgagcatct ttcggccgaa
    19021 ggcgacataa tctaaacact agttaaacct gatattcata cgcaaaggaa tctatgaagg
    19081 cttggcaaga aactcaactt caggcattgc agtctattca atgcgcacac cagctttttc
    19141 aaacgattct ttcgtttagt aaggaacttg gctttgacca ttgcgcctat ggcctgcgta
    19201 tgccacttcc cttaacgaac ccaaagacat tgatgtttaa taattaccca acagcttggc
    19261 aagcccaata tcaagccaaa aattatcttg caattgaccc cacggtgcaa cacgccatgc
    19321 gctcactttt gccaattata tggacagatg acttattcag tccagctagt gatctatggg
    19381 aggaagcgtg ctcatttgga ctgcgcgttg gctgggcgca atccagccgt gatttcaacg
    19441 ggtttgtcgg catgttaacg cttgcgcgct caaatgagcc gatcactgaa atagagcttg
    19501 atgccaacaa attcaaaatg gcctggctga cccaagtagc gcatttaggc atgtcgcggt
    19561 gcttgacgcc gaaattgatg caggaagtta atgtgacatt gtcaaaccgc gagatagagg
    19621 tgcttcggtg gacggctgat ggcaaaacat ctagcgagat ctcgtgcata ctcaatattg
    19681 cggagcgtac tgtcaacttt catatcaata atgtcgtcac taagttgaat gctgccaaca
    19741 aaacttcagc tacgataaaa gctgcaatgc ttggattgtt gtaa
//
