LOCUS       BGC0001485             10917 bp    DNA     linear   BCT 13-MAR-2018
DEFINITION  Actinoplanes tsinanensis chuangxinmycin biosynthetic gene cluster,
            complete sequence.
ACCESSION   BGC0001485
VERSION     BGC0001485
KEYWORDS    .
SOURCE      Actinoplanes tsinanensis
  ORGANISM  Actinoplanes tsinanensis
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Actinoplanes.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:39:49
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 10917
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..966)
                     /gene="cxm0"
     CDS             complement(1..966)
                     /codon_start=1
                     /gene="cxm0"
                     /note="Cxm0"
                     /product="tryptophanyl-tRNA synthetase"
                     /protein_id="AVL27075.1"
                     /transl_table=11
                     /translation="MFSLSGITPSGKAHLGNYLGAVRRWAAQSGPEDLYFVSNLHAMTT
                     KHDPERLQELTDHQLALLIAAGVPQERLFVQSDLIQEHMALTWLLECTCTFGEARRMVQ
                     FKEKSQGSNSVRLGLLTYPVLMAADILLHGASEVPVGHDQNQHVELARTLARRFNTDYG
                     EVFTVPQAVLPVAAARVRDLAAPTRKMSKSSSDGSGIVYVLDSPEAVRRKFQRAVTDGE
                     NTVRYAPDEQPGVANLLEIRAACTDTLPSDAAKGIDSYRDLKEAAAEAVISLIAPVRER
                     ALQLLEERSELAKIRAEGADRARARSRDRLDRALSLAGLK"
     source          1..10917
                     /country="China"
                     /db_xref="taxon:2039464"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Actinoplanes tsinanensis"
                     /strain="CPCC 240351"
     misc_feature    1..10917
                     /note="chuangxinmycin biosynthetic gene cluster"
     subregion       1..10917
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001485"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10917
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1177..2199)
                     /gene="cxm1"
     CDS             complement(1177..2199)
                     /codon_start=1
                     /gene="cxm1"
                     /gene_functions="regulatory (smcogs) SMCOG1014:LysR family
                     transcriptional regulator (Score: 174.4; E-value: 5e-53)"
                     /gene_kind="regulatory"
                     /note="Cxm1"
                     /product="LysR family transcriptional regulator"
                     /protein_id="AVL27076.1"
                     /transl_table=11
                     /translation="MHLAWVIVCSRLHTRQYPLRMGPYTDHMELDLRHLRYFVAVAEEG
                     GFTRAAARLHMTQPPLSVAIRQLERELGLQLLDRTGNRVELTSVGRDFLTHARNLLQQW
                     QVTVERMRQAGSQDVERLVVAFRPAVSRPLAHRTIELIREKHPEYQVVPRYVPWTEQTA
                     CLEAGDADVSFVLEPADYVGLERATVALLPRVVCLPSAHELASRDSVSIDDLSEVPIIR
                     PTGGSPEWSDFWGGEVCPGKRTWKEPPTATRLDEAIDLVALENAAALVPVSVMAVQHRQ
                     DVVFIPVTDVPAARLSLAWREGSDSELVRLAVRCAQAAAQDPAVRTLFGEPRPTGTAPA
                     "
     gene            2415..3905
                     /gene="cxm2"
     CDS             2415..3905
                     /codon_start=1
                     /gene="cxm2"
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 228.7; E-value:
                     1.5e-69)"
                     /gene_kind="transport"
                     /note="Cxm2"
                     /product="MFS transporter"
                     /protein_id="AVL27077.1"
                     /transl_table=11
                     /translation="MSYNGTSPRPPSISATMTLIATGVSVLSYALMQTMVVPALHVLQV
                     QLHTASTWSAWILSVFLLTSAASTPLLSRLGDRYSKRKVLLLVLTTYLIGTVGCAVAGN
                     IGVLIACRAVQGVSLAAIPLSFGILRDVLPEQRLRSGLGLVSGTIGVGAGIGLVVGGLV
                     VDHQSWRWLFAVAAVLILGAIGLVAKYVPDQRGEAGEPVDVPGAVLLALVLVALLLALT
                     KGTSWGWASTGTLALFGASAVLLGLLVVVERKSPAPLIDPAVVAGRSFVSVHGAAFVFG
                     VVSFVFYVLLPTYAQTAADQRLPGGGTIGYGLGADVTMAGLLLLPGSLVLLPAGPLAGL
                     LQRLTSVRATLASGFAVMAVGAISLWAWNANGWQVAVGYLVVGLGSGLVLSGLPSVISD
                     LTEARRTATANGVNTVVRTAGGVVGSQLAVALLAAWHISGSDTPARDGFTTAFWIAAAV
                     AAAGGLLCWVGIKTSTLRGPRMPGVTDLPRQSAGGVRP"
     protocluster    3318..10917
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[8317:10189]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      8318..10189
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    3318..10917
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     gene            3974..4690
                     /gene="cxm3"
     CDS             3974..4690
                     /codon_start=1
                     /gene="cxm3"
                     /note="Cxm3"
                     /product="metal-dependent protease"
                     /protein_id="AVL27078.1"
                     /transl_table=11
                     /translation="MAGVKDAQYVTAATDDGLGGTADSAALLDDLPVTVRFEIEPVRRF
                     LSSALGEYQKCLDSRDADGVPSHLPRASGLLFGQVGGAEIVISDVEFVPNVRDSDESVM
                     AEFEATIAPQFGDVYKNPGRGFWSDEQGVLQAIRQQSANGLELLGSIHSHPNWHEIGPP
                     HERRQRLSEHPTQMDEYLFRQSCWPVNVIWYVHESSGGIAHRVAAWRPGAEQCDRLDIR
                     IPAAIHEQFEVLLEEE"
     gene            4694..4996
                     /gene="cxm4"
     CDS             4694..4996
                     /codon_start=1
                     /gene="cxm4"
                     /note="Cxm4"
                     /product="sufur carrier protein"
                     /protein_id="AVL27079.1"
                     /transl_table=11
                     /translation="MPDVKLPAAFHVLTGGRRQLPVEGANIREVLVGLDQTCPGVLERL
                     MDQEGSVKRYVNVYRNDSDIRSLDGLETKVEHHDVIWIVPAVAGGSEAARAEESR"
     gene            4993..6207
                     /gene="cxm5"
     CDS             4993..6207
                     /codon_start=1
                     /gene="cxm5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 424.9; E-value:
                     4.9e-129)"
                     /gene_kind="biosynthetic-additional"
                     /note="Cxm5"
                     /product="cytochrome P450"
                     /protein_id="AVL27080.1"
                     /sec_met_domain="p450 (E-value: 5.6e-23, bitscore: 70.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDVIPTEFFTEPGSNPHATAAEYRSKCPVHRINVPPGADAYAVL
                     GNKVVEEALGDSRLSKQVENLPARYRDKAVASSLLVVGNLGFADAPKHTRLKKPISRAF
                     LPATVAQLRPRIQDIVDDLIDTFPENGEIDLLSSFALPMPLTVICEYLGIPVADRPLFL
                     EWSYILSQDPLQHDEAELKAASEEFTDYFTKLVAERRTDLRDDLLSEIIRARDAGVYSE
                     TELLSTLLLLIIAGHKTVANMIGNGTALLLRHPQQLEMLRATPELIPSAIEEILRYEGS
                     AAWASLRVAAEDMQLAGVDIPKGSFVHLSLSSAGRDPDVYDDPDGFDVTRSPNRHLSFG
                     HGPHFCIGAPLGRLQGEIAFSTLLRRLPRFELAVPPEEVAWLSDSSLSRGLEALPIRVG
                     ERLPR"
     gene            6204..7160
                     /gene="cxm6"
     CDS             6204..7160
                     /codon_start=1
                     /gene="cxm6"
                     /note="Cxm6"
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="AVL27081.1"
                     /transl_table=11
                     /translation="MTEQGGPSIAVVGAGGVGGYFGGLLAAAGHDVRFLARGENLAALR
                     RQGLRITNGSSDLRVPDVRASADPKDIGEVDFVLLCVKTSQLPAALDALGPLVGEHTAV
                     VTVQNGVEAPEQVAARIGRGRVLPGSVRVVASTAGPGELRHVGPPGALAFTEWDSTVSD
                     RVARLREVLRAASVSVPEPSDIWAGLWAKFLLVVPIGSLGAATGGATIGELRSRTGTRN
                     ILIAGMREIYETGIKLGIALPAAAVDTATELMDQQSPDVTSSLQRDILAGRPSELEAWT
                     GAVVRLARGAGLTAPVHEMLYELLATRESRTARSLQA"
     gene            7157..8245
                     /gene="cxm7"
     CDS             7157..8245
                     /NRPS_PKS="Domain: Aminotran_1_2 (32-347). E-value: 8e-46.
                     Score: 148.6. Matches aSDomain:
                     nrpspksdomains_cxm7_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="cxm7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019:aminotransferase (Score: 149.6; E-value: 2e-45)"
                     /gene_kind="biosynthetic-additional"
                     /note="Cxm7"
                     /product="PLP-dependent aminotransferase"
                     /protein_id="AVL27082.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.4e-46, bitscore:
                     148.6, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVRFAERSTLRDMRAYRDKESSNAEGSSRFTFDLSSNELVLPPL
                     PTVLAGIEKGLPRLARYPDPTARDLTEDIAGHLCVSPDEVAVGPGSAGVLQQILLALCG
                     KGDEVVHGWPGFDAYPLLVAISGATGVHVPLTASGGHDLDEIRTRVNARTRVVILCSPH
                     NPTGTVIDQDELHGFLRSLPAHVVAVLDEAYVEFDRGANPPGLPVLLSEHSNTVVLRTF
                     SKAYGLAGLRVGYAAGPRQVMATVRKTAIPFGVTRFAEQAAMLSLRSEDELCERLAAVA
                     AAREELTAELRELRLPVLLSRANFVWLPLASAAESFARTAATAGVKVRAFPGHGVRISV
                     GEAEAHRTLLAALGRADRGNWF"
     aSDomain        7253..8197
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_cxm7_Aminotran_1_2.1"
                     /evalue="8.00E-46"
                     /label="cxm7_Aminotran_1_2.1"
                     /locus_tag="cxm7"
                     /protein_end="347"
                     /protein_start="32"
                     /score="148.6"
                     /tool="antismash"
                     /translation="FDLSSNELVLPPLPTVLAGIEKGLPRLARYPDPTARDLTEDIAGH
                     LCVSPDEVAVGPGSAGVLQQILLALCGKGDEVVHGWPGFDAYPLLVAISGATGVHVPLT
                     ASGGHDLDEIRTRVNARTRVVILCSPHNPTGTVIDQDELHGFLRSLPAHVVAVLDEAYV
                     EFDRGANPPGLPVLLSEHSNTVVLRTFSKAYGLAGLRVGYAAGPRQVMATVRKTAIPFG
                     VTRFAEQAAMLSLRSEDELCERLAAVAAAREELTAELRELRLPVLLSRANFVWLPLASA
                     AESFARTAATAGVKVRAFPGHGVRISVGEAEAHR"
     gene            8318..10189
                     /gene="cxm8"
     CDS             8318..10189
                     /codon_start=1
                     /gene="cxm8"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277:Radical SAM
                     domain protein (Score: 168.4; E-value: 3.9e-51)"
                     /gene_kind="biosynthetic"
                     /note="Cxm8; SAM"
                     /product="B12-dependent radical S-adenosylmethionine"
                     /protein_id="AVL27083.1"
                     /sec_met_domain="TIGR03975 (E-value: 2.7e-174, bitscore:
                     570.1, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 7.3e-20, bitscore: 61.4,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLLMIAMPWQGLDTPSSALGVLGPCVRKNAADWTVDELYANLRW
                     AEYLMRESNGSVTCEDYGNIADQVFHGVGDWVFTPALYDVDSYQVDEYAKFLEQRDMDP
                     TLPVEMHKYARGFIRDLAAEIAADPPDVVGFTSTFMQNVPSLALARELKKLAPGIRTVL
                     GGSNCDGAQGPALHRNFEQLDFVISGEGERALPALLNRIIRGESLADVPGLSWRGDDGH
                     PVVNPPATAALPFAMVPAPGYDSYFQALERSPVRHHVRPMLVLETSRGCWWGEAHQCTF
                     CGLNGSNIDFRSKAPERIAQEVRELAERHQILDLVMVDNILDMKYLNTAMPEIAALDCD
                     LRIHYEIKSNMNREQLSRLKEANVLFVQPGIESLSSHVLRLMDKGVSAAHNVRMLRDGQ
                     DLGLNVTWSILYGFPGETEDDYRGLLKKLATLEHLEPPTGAWRIALERFSPYFEDPTQG
                     FMFRRPSEIYDFIYQIPQDQLYDMVFFFDTSVRGISGPIEDEMKQACEEWAKAYPQGTL
                     SYWTDDRGRVVIEDRRASWPTEVIELDEVRSNVYLGMFQCAAREGIRRRLADSGHVVGE
                     AELEEMLRYFVDRGLAFEDEGRYVSVALGVDPYRRKLVGGKEVAASL"
     gene            10186..10917
                     /gene="cxm9"
     CDS             10186..10917
                     /codon_start=1
                     /gene="cxm9"
                     /note="Cxm9"
                     /product="hypothetical protein"
                     /protein_id="AVL27084.1"
                     /transl_table=11
                     /translation="MSALLDIDELKARDSDEGRVPAGGRPATETLTLGLDRAELPVATE
                     LAALLHRVPVAGVRLPEPADFSALPSHVIVRIIALIRECSSIGTRVTWSLTLGAEQLDL
                     VPRLDHLPAPDSITVLETGHPSVGEWRSSSNFGLLYFRKGPKFLSVVDQRPESSREIIV
                     DDPTQMAVFLLGLEGCAWAEVTRNSQFAAAARDLVNKGLVMRVGDHCVTLPVHMRSWPL
                     GAALLGGTLAAAGKKSDGATE"
ORIGIN
        1 ctacttcaga ccggcaaggc tgagcgcacg atccaagcgg tctcgtgacc gcgcccgagc
       61 acggtcggcc ccctcagccc ggatcttcgc cagctccgat cgctcttcga ggagctgcag
      121 tgcccgctca cgcaccggtg cgatcaggga gatcactgcc tctgcggctg cttccttgag
      181 gtcacggtag gaatcgatac ccttcgccgc atcgctcggg agcgtgtcag tgcaggcagc
      241 cctgatctcc aggaggttgg caacgcccgg ctgttcgtcc ggggcgtagc ggacggtgtt
      301 ttctccgtct gtcactgcgc gttggaactt ccggcgtacg gcctccgggc tgtccaggac
      361 gtagacgatg ccgctgccgt ccgaggacga cttcgacatc ttccgcgtag gggcagcgag
      421 atcacgtacc cgggctgcgg ctacgggcag gacggcttgc ggaaccgtga acacctcgcc
      481 gtagtccgtg ttgaaccgcc gcgccaaggt ccgggccagc tccacatgct ggttctgatc
      541 gtgaccgacg ggcacctctg aagcgccatg aagcaggatg tccgccgcca tgaggacggg
      601 gtaggtgagc agaccaaggc gtacggagtt gctcccctgg gacttctcct tgaactgcac
      661 catccttcga gcctccccga aggtgcaggt gcactcgaga agccacgtca acgccatgtg
      721 ctcctggatg aggtccgact gcacgaagag acgttcctgg ggtacgcccg ccgcgatgag
      781 taaagcgagt tggtggtcgg tcagttcctg gagacgttcg gggtcgtgct tggtcgtcat
      841 ggcgtgcagg ttgctgacga aatacaggtc ttcggggccc gactgtgctg cccaacgacg
      901 cactgcccca aggtagttac ccaggtgcgc cttacctgaa ggcgtgatcc cggaaagact
      961 gaacattggc cctgatcctc tcgtcttgac cgcatcgacg aggcgggccg accaagttca
     1021 gcagcccgtc cctcggagcg gctggctggc tcggtcgagc gcaggttctt gggccgccct
     1081 aggcggccca ccaacaaaga ttcctgcgca acatgatcgc gaacgtacca caactggcag
     1141 gggccatcag gaagttcctg acagcccctg ctctcatcag gccggagcgg ttccggttgg
     1201 tcgaggttct ccgaagagcg tcctgacggc tggatcctgg gctgcggcct gagcgcacct
     1261 gacggcgagg cgtaccagtt cggagtcgga accctcacgc caggcaaggg acaaccgggc
     1321 ggcaggcaca tccgtcacag ggatgaagac gacgtcctga cggtgctgga ctgccatgac
     1381 agagacgggg acgagcgcgg ctgcgttctc gagggccacg aggtcgatgg cctcgtcgag
     1441 gcgcgtcgct gtgggaggtt ccttccaggt gcgcttgccg gggcacacct caccacccca
     1501 gaagtcggac cactcgggcg acccgccggt ggggcgaatg atcggaacct cgctcaggtc
     1561 gtcgatcgac acggagtcac gactggccag ctcgtgagcc gatggcagac agacgacccg
     1621 gggtaacagg gccacggtgg ccctctcgag gcccacgtag tccgcgggct ccagcacgaa
     1681 ggacacgtca gcgtcccctg cctccaggca tgctgtctgt tcggtccacg gtacgtaccg
     1741 gggcactacc tgatactcag ggtgcttttc gcggatgagt tcaatggtcc ggtgtgccag
     1801 agggcggctg acggccgggc ggaacgcgac gacgagccgt tcgacatcct gcgaccccgc
     1861 ctgccgcatc ctctcgaccg tgacctgcca ctgctgcaac aagttcctcg cgtgagtcag
     1921 gaagtcgcgc ccgaccgacg tgagttcgac tctgttgccc gttctgtcca aaagctggag
     1981 acccagctct ctttcgagtt gacgaatcgc cacgctcaac ggcggctgtg tcatgtgcag
     2041 gcgggccgcg gctcgcgtga acccgccttc ctcggctacg gcaacgaagt acctgaggtg
     2101 ccgcaaatcc agttccatat gatcagtata tggccccata cgcaacgggt attggcgtgt
     2161 atgtaggcgg ctacatacga tcacccatgc aaggtgcagc tatcgaatct cagccttgac
     2221 tgcggggcct gtggaaaatt ccaccggagg tcttcttgtc tttgccgtac gaagtcgctt
     2281 tcagcgggtg tatcgcccca aacacaattg acggattctt tgcgcaggca gtgtttaagc
     2341 ctcagtgatg tggctccggc cagtggtctc gtgactcagt aaccgccccg accacatccc
     2401 cacgggggtc ccccttgtcc tacaacggca cttcgccacg tccaccgtcg atcagcgcga
     2461 cgatgacgct gatcgccacc ggcgtctccg tgctgtcgta cgcactcatg cagaccatgg
     2521 tcgtcccggc cttgcacgta ctacaggtgc agttacacac ggcctcgacg tggtcggcgt
     2581 ggatcctgag tgtgttcctg ctgaccagtg cggcgagcac gcccttgctg agccggctgg
     2641 gggaccgcta cagcaagcgc aaagttttgc tgctggttct caccacctac ctgatcggca
     2701 cggtcggttg tgctgtggca ggcaatatcg gggttctgat cgcatgccgt gccgtgcagg
     2761 gagtcagcct cgcggccatc cccctgtcct tcggcatcct gcgcgacgta cttcctgagc
     2821 agcggctgcg ttcgggcctc gggctggtgt cagggaccat cggtgtgggg gccggcatcg
     2881 gcctggtcgt gggaggtctg gtcgtcgacc accagtcatg gcgctggctg ttcgccgtcg
     2941 ccgcggtact gatcctgggc gcgatcgggc tcgttgcgaa gtacgttccg gaccagcggg
     3001 gcgaagccgg cgaaccggtg gacgtgcccg gggcggtgct cctggccttg gtcctcgtcg
     3061 cgctgttgct cgcgttgacc aagggcacct catgggggtg ggcgtccaca ggaacgctgg
     3121 ctctgttcgg ggcctccgcg gttctcctgg gattgctggt cgtagtcgaa cgaaagtcgc
     3181 ccgccccctt gatcgacccc gcggtcgtgg ccggtcgctc cttcgtctcg gtgcacggtg
     3241 cggcgttcgt gttcggcgtg gtctcgttcg tgttctacgt cctgctgccg acgtacgccc
     3301 agaccgcggc ggatcagcgg ctaccgggcg gagggaccat cggctacggg ctcggggccg
     3361 atgtcaccat ggcgggtctg ctccttctgc ccggctcatt ggtgctgttg cctgcgggac
     3421 cactagcagg tctgctgcag cgcctgacgt ccgtacgcgc gacgctcgca tcaggcttcg
     3481 ccgtgatggc ggtcggggcg atctcgctct gggcgtggaa tgccaacggg tggcaagtgg
     3541 cggtcggtta cctggtcgta ggtctcggtt ccggactggt gctgagcgga ctcccgtcgg
     3601 tgatcagcga cttgacggag gcccgacgca ccgcaaccgc caatggcgtc aacacggtgg
     3661 tgcgcacggc aggcggcgtc gtaggcagtc agctggctgt cgccttgctg gctgcctggc
     3721 atatctccgg ttccgatacg ccggcgcgcg acggcttcac caccgccttc tggatcgctg
     3781 ccgccgtagc ggcggccgga gggctgttgt gctgggtcgg catcaagacc tccacgctcc
     3841 gcggccctcg tatgccaggg gtgactgacc tgcctcgcca gagcgcaggg ggcgtacgcc
     3901 catgaacgct ccgcaattcc accgactgcc cagggcctaa ggatttcacc aggaaatctc
     3961 agggagggtg taaatggcag gcgtgaaaga tgcccagtat gtgacagctg ctacggatga
     4021 cggtctgggt ggcacagcag acagtgcagc cctgctcgac gatctgccgg tgaccgtccg
     4081 atttgaaatc gaacccgtca ggcgtttcct gagcagcgca ctgggggagt accaaaaatg
     4141 cctggacagc cgcgacgccg atggcgtccc gagccatctg ccgcgtgcat cgggtctgtt
     4201 gttcgggcag gtgggcggcg ctgaaatagt gatcagcgat gtggaattcg tgccgaatgt
     4261 ccgggacagt gacgagagtg tcatggccga attcgaagcg acgatcgccc cgcagttcgg
     4321 cgacgtgtac aagaatccgg ggcgaggatt ctggtccgat gaacagggcg tcttgcaggc
     4381 gatcaggcaa cagtcggcga acggcctgga gttgctgggc tccatacatt cacatccgaa
     4441 ctggcatgaa atcggcccgc cgcacgaacg gcgccagcgc ctgagcgaac atccgacaca
     4501 gatggatgaa taccttttcc ggcagtcatg ctggccagtg aacgtcatct ggtacgtgca
     4561 tgagagtagt ggcggtatcg cacatcgggt agcggcctgg cggcccggtg ccgagcaatg
     4621 cgacaggctt gacatacgga ttccggcggc gatccatgag cagttcgaag tcctactcga
     4681 ggaggaataa gaaatgcccg atgtcaaact tcccgcagcc ttccacgtcc tgaccggcgg
     4741 tcggcggcag ttgcctgtcg agggcgccaa tatccgggag gtccttgtcg gtctcgacca
     4801 gacctgtccg ggggtcctcg agcgactcat ggaccaggaa gggtccgtga agcgctacgt
     4861 caatgtctac cggaacgaca gcgacatcag gagcctcgac ggcctcgaaa cgaaagtgga
     4921 gcaccacgac gtcatctgga tagtgccggc ggtagcaggt ggcagcgaag ccgcgcgagc
     4981 cgaggagtca cgatgaccga cgtgattccc acggaattct tcaccgagcc cggttcgaat
     5041 ccgcacgcga cagccgcgga gtacaggtcc aagtgtccgg tccatcggat caatgttccc
     5101 cccggcgccg acgcgtacgc ggtcctcggg aacaaagtcg ttgaggaagc gctcggcgac
     5161 tcgcgcctct ccaagcaagt cgagaacctg cccgcccgct atcgagacaa ggccgtggcc
     5221 agcagtctcc tcgtggtcgg caacctggga ttcgccgacg cgcccaaaca cacccgcctg
     5281 aagaagccca tcagccgggc attcctgccg gccacggtcg cccaactacg cccgcgcatc
     5341 caggacatcg tcgacgatct catcgacacc tttccggaga acggcgaaat cgacctgctc
     5401 agttccttcg ctctgccgat gccgctcacc gtcatctgcg aatacctggg gataccggtg
     5461 gcggaccggc cgctgtttct ggagtggagc tacatcctca gccaggaccc gttgcagcac
     5521 gacgaggcgg agctgaaagc cgcgagcgaa gagttcacgg actatttcac caagctcgtg
     5581 gccgagcggc gcacggacct gcgggacgac ctgctgagcg agatcatcag ggccagggac
     5641 gcaggcgtat acagcgaaac agagctcctt tcgacgctcc tcctgctgat catcgccggt
     5701 cacaagacgg tcgccaacat gatcggcaac ggcacagcac tgttgctccg ccatccccag
     5761 cagctcgaga tgctccgagc cacccccgag ctgatccctt cggcgatcga ggagatcctg
     5821 cgctacgaag ggtcggccgc ctgggcctcg ctgcgggtcg cggcggagga catgcagctc
     5881 gcgggagtgg acatacccaa ggggagcttc gtacacctgt cgctgtccag cgcgggtcgt
     5941 gatcccgacg tgtacgacga cccggacggt ttcgacgtga cgcggtcacc gaaccgccat
     6001 ctgtccttcg gccacggtcc ccacttctgc atcggcgctc cgctgggccg actccagggc
     6061 gagatcgcct tctccacgct gttgcgccga ctgccgcgat tcgagctcgc cgtgcctccg
     6121 gaagaggtcg cctggctctc cgacagttcg ctcagccggg gcctcgaggc cctcccgata
     6181 cgagtgggag agaggttgcc gcgatgacgg aacaaggcgg tccgagcatt gctgtggtgg
     6241 gagccggcgg agtaggcggc tatttcggcg gcctgctcgc cgccgccggg catgatgtgc
     6301 ggttcctggc ccgcggcgag aacctcgccg cgctcaggcg acaggggctg cgcatcacca
     6361 acggctcaag tgacttacgt gtgccggacg ttcgggcgtc ggccgacccg aaggacatcg
     6421 gcgaggtcga cttcgtactg ctctgcgtca agacctcaca gctgccggcg gccctcgacg
     6481 cgctgggccc gctggtcggc gagcacactg ccgtggtcac ggtgcagaac ggggtggaag
     6541 cccctgagca ggtcgcagcc aggatcggcc gcggtcgggt actccccggc agcgtcaggg
     6601 tcgtggcctc gacggccggt ccgggcgagt tgaggcacgt gggtcccccc ggcgctctgg
     6661 ccttcaccga gtgggacagc actgtgtccg accgggtggc acgactgcgc gaggtgctgc
     6721 gtgctgcctc ggtgtccgtg cccgagccga gcgacatctg ggccggcctt tgggcgaagt
     6781 tcctgttggt ggtcccgatc ggcagccttg gagccgccac cggcggggcg accatcggcg
     6841 agctccggtc gcgcaccggc acccgcaaca ttctgatcgc cggcatgcgg gagatctacg
     6901 agaccgggat caagctcgga atcgcgctgc cggcagctgc cgtggacacc gcgacagagc
     6961 tcatggacca gcagtcgccc gacgtcacct cctcgctgca acgggacatc ctggcgggac
     7021 ggccgtcaga gctcgaagcg tggaccgggg ccgtggtccg cctcgcccgc ggggcgggcc
     7081 tgaccgcgcc ggtccacgaa atgctctacg agctgctcgc cacccgcgaa tcacgcacgg
     7141 cgaggagtct gcaggcgtga acgtgagatt cgcagaacgc agcaccctgc gggacatgcg
     7201 ggcctaccgg gacaaggagt cgtcgaacgc cgaaggcagc tcgcggttca ccttcgacct
     7261 gtccagcaac gagctggttc tccccccgct gcctaccgtg cttgccggta tcgaaaaagg
     7321 cttgccgcga cttgctcgtt accccgaccc cacagcacgg gacctgaccg aggacattgc
     7381 cgggcacttg tgcgtctccc cggatgaggt cgcggtcggc cccggaagcg cgggcgtgct
     7441 ccagcagatc cttctcgcac tgtgcggcaa gggcgacgaa gtcgtccatg gctggccggg
     7501 attcgatgcc tatccgctgt tggtcgccat ttcaggtgcc accggagtcc acgtgcccct
     7561 gactgcgtcc ggcggccacg atctcgatga gatccgcacc cgggtgaatg cgcggaccag
     7621 ggtggtgatc ctgtgctccc cgcacaatcc gaccggaacc gtgattgacc aagacgagct
     7681 gcacggtttc ctgcgttcgc tgccggctca tgtggtcgcg gtcctcgacg aggcgtacgt
     7741 ggagttcgac cggggcgcca atcctccggg tctgccggtg ttgctgagcg agcacagcaa
     7801 caccgtggta cttcgaacgt tctccaaggc ctatggcctt gccggcctac gggtcggtta
     7861 cgcggccggg ccacggcagg tcatggccac cgtccgcaag acggcaatcc ctttcggagt
     7921 gacgcgcttc gcggaacaag ccgcaatgct ctcgctgcgc agcgaggacg aactgtgtga
     7981 acgtctggca gcagtggctg cggcacgcga agaactgacc gcggaactca gggaactgag
     8041 gctgcccgtc ctgctttccc gagccaactt cgtctggctc ccgctcgctt cggccgccga
     8101 gtctttcgcc cggactgcgg ctaccgcagg ggtcaaggtc cgagcctttc cggggcacgg
     8161 tgtacggatc tcagtgggag aggccgaggc acatcggacg ctgttggcgg cgctcggccg
     8221 ggcggaccgt gggaactggt tctgagaact ctctgcggac aattcttttg cccgtcctca
     8281 tacaactcat cgacagcaac tctggggtgg gaagtctgtg aaactcctga tgatcgccat
     8341 gccgtggcaa gggctcgaca cgccgtccag cgcgctgggc gtactggggc catgcgtccg
     8401 caagaacgcc gccgactgga ccgtcgatga gctgtacgcc aacctccgct gggccgaata
     8461 cctgatgcgg gagagcaacg gctccgtcac ctgcgaggac tacgggaaca tcgcggatca
     8521 agtcttccac ggcgtagggg actgggtgtt caccccagcg ctgtacgacg tcgacagtta
     8581 tcaggtcgac gagtacgcga agttcctcga gcagcgggac atggacccga cacttcccgt
     8641 tgagatgcac aagtacgccc ggggattcat ccgggatctc gcggccgaga tcgctgccga
     8701 tcctcccgac gtggtcgggt tcacgagcac cttcatgcag aacgtcccgt cgctcgcact
     8761 ggccagggag ctgaagaaac tcgcgccggg catccgcacg gtccttggcg gcagcaactg
     8821 cgacggagca caaggtcccg cgttgcaccg gaatttcgag caactcgact tcgtgatcag
     8881 cggtgagggc gaacgtgcgc tgcccgcgtt gctgaatcgc atcatccggg gcgagagcct
     8941 cgccgacgtg ccggggctca gctggagggg ggatgacggg catccggtgg tgaatccacc
     9001 ggccacggcg gcgctgccgt tcgccatggt gcccgcgccc ggttacgaca gctactttca
     9061 ggccctcgaa aggtcacccg tccgtcacca cgtccgtccg atgctggtcc ttgagacctc
     9121 ccgtggttgc tggtggggag aggctcacca atgcacgttc tgcggcctga acggatcgaa
     9181 tatcgacttc cggagcaagg cccctgagcg catcgcccag gaagtccggg aactggccga
     9241 gcgacaccag atcctcgacc tggtcatggt ggacaacatc ctcgacatga agtatctcaa
     9301 tacggccatg cccgagatag ctgccctcga ctgcgatctg cgaattcact acgagatcaa
     9361 gtccaatatg aaccgggagc agttgagtag gctgaaagag gcgaacgtcc tcttcgtgca
     9421 gcccggtatc gagagcctga gcagccatgt gctgcgcctc atggacaagg gcgtcagtgc
     9481 cgcgcacaac gtacggatgc tgcgtgacgg acaggatctc gggctcaacg tgacatggag
     9541 catcctctac ggcttcccgg gcgagaccga ggacgactac cgcgggctgc tcaagaaatt
     9601 ggccacgctc gagcacctgg agccgccaac cggggcttgg cgcatagccc ttgagcggtt
     9661 cagtccctac ttcgaggacc ccacccaggg gttcatgttc cgccgcccct cggaaatata
     9721 cgacttcatc taccagatcc cgcaggatca gctgtacgac atggtgttct tcttcgacac
     9781 cagcgtccgg ggaatctcag ggcccatcga ggacgagatg aagcaggcct gcgaggaatg
     9841 ggccaaggcc tatccgcagg gcaccctttc ctactggacc gatgaccgag gccgggtcgt
     9901 catcgaagac cgccgtgcca gctggccgac ggaagtgatc gagctggacg aggtccgcag
     9961 caatgtgtac ctcggcatgt tccagtgcgc cgcccgcgag ggcatacgcc ggcggcttgc
    10021 cgacagcggg cacgtcgtcg gggaagcgga actcgaggaa atgctccgct acttcgtcga
    10081 tcgcggcctg gcattcgagg acgaggggcg ctacgtaagc gtcgccctcg gggttgaccc
    10141 gtatcgccga aagctggtcg gcggcaagga ggtggcggct tcgctatgag cgctttgctg
    10201 gacatcgatg aactcaaggc ccgagattcg gacgagggac gggtaccggc cggtggccgt
    10261 cccgcgaccg agacgctgac cctcggactc gaccgggccg aacttcccgt cgccaccgaa
    10321 ctggcggcgc tactgcaccg ggtgcccgtc gccggggtac ggctgccgga gcccgccgac
    10381 ttctcggcac tccccagtca cgtgatcgtg cggatcatcg cgttgattcg cgaatgttcc
    10441 tcgatcggca ccagagtcac ctggtcactg accctgggcg ccgaacagct cgacctggtc
    10501 ccccgtctcg atcaccttcc tgcccccgac agcatcacgg tgctggagac agggcatccg
    10561 tccgtcggtg agtggcggtc ctccagcaat ttcggcctcc tctacttccg taagggcccg
    10621 aagttcctgt ccgtcgtcga tcagcgcccc gaatccagcc gcgaaatcat cgtggacgat
    10681 cccacccaga tggccgtctt cctcctgggc ctggagggat gcgcgtgggc cgaggtgacc
    10741 cgaaactcgc aattcgccgc cgccgcacgc gatttggtga acaagggcct tgtgatgcgc
    10801 gtcggggatc actgcgtgac cctccccgtg cacatgcgtt catggcccct gggcgcggcc
    10861 ctattgggag gaaccttggc cgcagccggc aagaaatcgg acggtgctac ggagtag
//
