LOCUS       BGC0001487              9112 bp    DNA     linear   BCT 06-MAY-2018
DEFINITION  Streptomyces cyaneofuscatus strain Soc7 3,7-dihydroxytropolone
            biosynthetic gene cluster, complete sequence.
ACCESSION   BGC0001487
VERSION     BGC0001487
KEYWORDS    .
SOURCE      Streptomyces cyaneofuscatus
  ORGANISM  Streptomyces cyaneofuscatus
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 01:59:16
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9112
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..480)
                     /gene="trlA"
     CDS             complement(1..480)
                     /codon_start=1
                     /gene="trlA"
                     /product="enoyl hydratase"
                     /protein_id="AWF83805.1"
                     /transl_table=11
                     /translation="MRHFEHFRVGSTYELGTTRLTSEDIIGYARVWDPMPFHLDPEAAA
                     RSPFGGLVASGWHTGAVVMGQFVRALLAGSACQGSYGMDEVRFLRPVRPGDDVRGRATV
                     EEAAPHPRRPDTGTVRFVVEADNQRGEALYRMRTRLLFGRAEAGGAQREEVAVRR"
     source          1..9112
                     /db_xref="taxon:66883"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces cyaneofuscatus"
                     /strain="Soc7"
     misc_feature    1..9112
                     /note="3,7-dihydroxytropolone biosynthetic gene cluster"
     subregion       1..9112
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001487"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9112
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(530..1984)
                     /gene="trlB"
     CDS             complement(530..1984)
                     /codon_start=1
                     /gene="trlB"
                     /product="DAHP synthase"
                     /protein_id="AWF83806.1"
                     /transl_table=11
                     /translation="MESLEMDENSLADPSVEAWRALPVAQQPDWPDPTALRTTLSALSA
                     APPLVFGQECDTLLDRLGEVALGRGFLLQGGDCAETLDGVGAESVRNKLEVLLQMAAVL
                     TYAAGVPVVKVGRIAGQYAKPRSQPTETRGGVTLPSYRGDAVNGLEFTAEARTPDPARL
                     ERVYQASAATLNLLRALTAGGHADLRRVHEWNRDFVALSAAGHRYERLADEIDAALSFI
                     RACGMSTSALRTTEVFASHEALLLDYESALVRTDPDTGRRYALSGHLLWIGERTRRLDG
                     AHVAFAAGVSNPLGVKLGPDAAVDDVRALVDRLDPLRTPGRLTLIARMGRGLVRDRLPA
                     LVEAVRAEGARVVWACDPMHGNTFTAPSGHKSRRFEDVLDEITGFFEVHRALGTHPGGV
                     HVELTGEDVTECVGGSDEVLSDDLPLRYETACDPRLNRGQSMDLAFRVAEWFSAERRRL
                     GRPRPEPALIRPVGAPVYAAHAASPG"
     protocluster    1536..9112
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[6535:7297]"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6536..7297
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     cand_cluster    1536..9112
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     gene            2248..3840
                     /gene="trlC"
     CDS             2248..3840
                     /codon_start=1
                     /gene="trlC"
                     /product="Tropolone 3,7-monooxygenase oxidase component"
                     /protein_id="AWF83807.1"
                     /transl_table=11
                     /translation="MSSTVNPSGSVSRPQTGDEYLESLRDGREVRIYGEKVQDVTAHPA
                     FRNNSRMVARLFDALHDPERNSKLVVPTDTGNGGFTHPYFKAPYTSGDLRTAARAMEEW
                     ARMTYGWLGRTPDFKAAFLSTLGSNKEHYAPFEDNASAWYRKAQENVLFIGHGIVNPPI
                     DRNRGLTELKDVMLTVDRETDAGLVVSGAKVVGTGAALTQHIFIGSYGVIPKGAKEFSA
                     YFIVPTDSPGLKIISRPSYEFASATVSSPFDQPLSSRLDENDGILVFDQVLIPWENVFC
                     YDVDKANDFFYGSGFFYRAMFHACVRFAVKMDFLTGLLAKCLETTGTSEFRGVQSRLGE
                     VLAYRNMFWALVDSMAENPTKWPDGTVTPNGETALAFRVLSSSIFPKVREIFLRDLGSA
                     LIYNNSHALDWSNPEVRPYLDKYVRGLGVEAIDRVKLMKLAWDAVGTEFGARWELYEMN
                     YAGNHEQIRFEALHAQQVSGKFDDYKQLVDRCLSEYDEHGWKVPDLVDPKDTTVIGAGA
                     PDTGTTAPRPAAR"
     gene            3837..4394
                     /gene="trlD"
     CDS             3837..4394
                     /codon_start=1
                     /gene="trlD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1160:flavin reductase domain protein FMN-binding
                     (Score: 77.8; E-value: 1.3e-23)"
                     /gene_kind="biosynthetic-additional"
                     /product="Tropolone 3,7-monooxygenase flavin reductase
                     component"
                     /protein_id="AWF83808.1"
                     /transl_table=11
                     /translation="MSSHTPAPGGPTAPPGAPVLPDQAQLRHVLGQFVTGVCAVSTRFD
                     TRSGVRHDAIAVNSFTSVSLDPPLVSMYLRDDSTFLRRVRTSGQWAVSFLGNRARSLVG
                     VLTAPAARRPPVEQAADWAVGPHTGCLVLPDGPGTLECALHLTQALGDHVLVVGRVLGA
                     VGRDHEPLVFHRGAFTEVAPAP"
     gene            4439..5641
                     /gene="trlE"
     CDS             4439..5641
                     /codon_start=1
                     /gene="trlE"
                     /gene_functions="other (smcogs) SMCOG1087:hypothetical
                     protein (Score: 333.4; E-value: 3.4e-101)"
                     /gene_kind="other"
                     /product="Tropone 2-monooxygenase"
                     /protein_id="AWF83809.1"
                     /transl_table=11
                     /translation="MARKRPYVAVVGGGIGGLAVALGLRRQGVEAVVHEQAHALSHQGA
                     GIAIGANGHRALRELGVAKRLTASAARPSRADFRHWRTGRSMVSHRLTGLYEERFGAPF
                     WTVERAAVQQALLAELGPRHVRLGARCTGVDRTADGAVIRFEDGGEAEADAVVGADGIH
                     SAVRHSLFGPQEAVFSGTSGYRALVPMDRLRHVPELAEPVLWLWLGPGRHFIAYPVADG
                     SALNFLAVVPDRTWTVESWSTEGDAAELRAAFDGWHPFVTEVLGACERPGRWALYDREP
                     QRVWSSGAVTLLGDAAHAMLPHHGQGANQALEDAVVLAHFLARTDTGGVPSALRAYERL
                     RRPRTRLLQAGSRKNAGCFQLPDGPQAEARNARLATLPDDVAWIHGHDILGSLPVATSP
                     A"
     gene            5697..6308
                     /gene="trlF"
     CDS             5697..6308
                     /codon_start=1
                     /gene="trlF"
                     /product="1,4,6-cycloheptatriene-1-carboxylic acid
                     decarboxylase"
                     /protein_id="AWF83810.1"
                     /transl_table=11
                     /translation="MARTYSFEGNVPVVHPTAFVHPDAVLIGSVDIGPGCYVGPLASLR
                     GDFGHIELRAGSNVQDGCVLHCFPGADTVVEEDGHVGHGSVLHGCRVGRDSLIGMKSVL
                     MDGVVVGTRAFVGAGSFVKSRFQVPERHLVAGSPAKVVRELTADEIAWKGNGTAQYQKL
                     AQRCLTGLHAAEAATERTAPAAPAEPGEHEHVTLHAYRSR"
     gene            6536..7297
                     /gene="trlG"
     CDS             6536..7297
                     /codon_start=1
                     /gene="trlG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /product="putative A-factor biosynthesis hotdog
                     domain-containing protein"
                     /protein_id="AWF83811.1"
                     /sec_met_domain="AfsA (E-value: 3.3e-14, bitscore: 42.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSSATSRLVVGDRFRDFAEAVGAETFSRLARSLDAGDLDAVEGP
                     LRVVTGQGVGEFEISYLEDAVRRRQLDDRIELVHSAGQPARRQELHKAREDNVLIAGLT
                     QVDDTAYEAALRLHDHNELLVDVQDRVHVQGMVAVEAARQMFVAVVERYFTARWPQQRY
                     YIVLNSINTTFSNFLFPVGATVRLTVDESEVSEPSRLTFRTTVEVEQAGRPAAAVTIDA
                     AAFAPGLLEEKELRRAKSAVASTMDAALAIA"
     gene            7299..8507
                     /gene="trlH"
     CDS             7299..8507
                     /codon_start=1
                     /gene="trlH"
                     /product="Bifunctional prephenate dehydratase and
                     chorismate mutase"
                     /protein_id="AWF83812.1"
                     /transl_table=11
                     /translation="MRTPTPDTRTRPAPGRLPYPEWRFAYLGPEGTFTELALRSLPEAT
                     GARLSPAATATEALTLVCRGAADAAMLPVHNTVAGVVADTVRALAESPALTVLREVVLP
                     VEFALLVRPGTRRARIRTVSGHPHAGAQVGGWLDSRAPQARWTPAPSNAEAARRVRDRE
                     CDAAVAGEFTAAHYGLQVLDAGIQDTAGAVTRFLLCARPGRWSGPRPAADRTSLVGRLA
                     ARPEEAGGLLDGLRSHPFLRSVSLRTVSDGRSGSVLFADCPGGAGQAATARAVGLLRLR
                     LPGLRVLGPYPSATHVPSYERSETMHTETAAEAGLSAETGSRDIARLRDRIDSLDHLLI
                     GTLRERLEASREIQRIRTRSGGSQVDPGREAAVRGRYAEELGEGGTGVAEALLDMCRGR
                     SPR"
     gene            8483..9112
                     /gene="trlI"
     CDS             8483..9112
                     /codon_start=1
                     /gene="trlI"
                     /gene_functions="regulatory (smcogs) SMCOG1057:TetR family
                     transcriptional regulator (Score: 107.6; E-value: 1.7e-32)"
                     /gene_kind="regulatory"
                     /product="TetR family transcriptional regulator"
                     /protein_id="AWF83813.1"
                     /transl_table=11
                     /translation="MPGAVTTVNPPPTTTATSSPRPRRGREDVLTAATALFHERGYDAT
                     SMSDIAARLGFTKAALYRHVTGKAELLRAITRPVRADVRALLAASTAGDDRPVDQLSEL
                     LRGLAGAAAADPGRYVLFWGPDGERSPHGPDAVCRAAVVQRLTELLERAADRGEIRDGI
                     VPHLAARLLVGAVTGPGRPVSPSAVDVLLDGPVRRLNDGAGRGRGR"
     misc_feature    8795..8797
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 tcagcggcgt acggccactt cctcgcgctg ggcgccgccg gcctccgcgc ggccgaacag
       61 cagccgcgta cgcatccggt acaacgcctc gccccgctgg ttgtccgcct cgacgacgaa
      121 ccggaccgtg cccgtgtccg ggcgccgggg atggggtgcg gcctcctcca cggtggcccg
      181 tccgcgcacg tcgtcgccgg ggcgcaccgg gcgcaggaac cggacctcgt ccatgccgta
      241 ggagccctgg caggcggagc cggcgagcag ggcgcggacg aactgcccca tgaccaccgc
      301 gccggtgtgc cagccgctgg ccaccaggcc gccgaagggg gagcgggccg ccgcctcggg
      361 atccaggtgg aagggcatcg ggtcccacac ccgcgcgtag ccgatgatgt cctcgctcgt
      421 gagccgggtg gtccccagct cgtaggtgct gccgacgcgg aagtgctcga agtgccgcac
      481 gggggtgctc cttcgtctcg ggggtctcgc gggggccggg gcccggcact cagccggggg
      541 acgccgcgtg ggccgcgtac accggcgcgc ccaccgggcg gatcagggcg ggctccgggc
      601 gggggcggcc caggcggcgc ctctcggcgc tgaaccactc ggcgacccgg aaggccaggt
      661 ccatcgactg cccgcggttc aggcgggggt cgcacgccgt ctcgtaccgc agaggcagat
      721 cgtcggagag gacctcgtcg ctcccgccca cgcactcggt gacgtcctcc ccggtgagct
      781 ccacgtgcac cccgccgggg tgcgtgccca gcgcccggtg gacctcgaag aagcccgtga
      841 tctcgtccag gacgtcctcg aagcgccggc tcttgtggcc gctcggcgcg gtgaaggtgt
      901 tgccgtgcat cgggtcgcac gcccacacca cccgggcgcc ctccgcgcgc accgcctcca
      961 ccagggcggg cagccggtcg cgtaccaggc cccgccccat ccgcgcgatc aacgtgagcc
     1021 ggcccggggt gcgcagcgga tccagccggt ccaccagggc ccgtacgtcg tcgacggcgg
     1081 cgtcgggacc cagcttgacg ccgagcgggt tgctgacccc ggcggcgaac gcgacgtgcg
     1141 cgccgtccag ccggcgggtg cgctcgccga tccagagcag gtgcccgctc agggcgtacc
     1201 gccggcccgt gtcggggtcc gttcgcacca gggcggactc gtagtcgagg agcagcgcct
     1261 cgtggctggc gaacacctcc gtggtgcgca gcgccgaggt gctcataccg caggcccgga
     1321 tgaacgacag cgccgcgtcg atctcgtccg ccagccgctc gtagcggtgt cccgccgcgg
     1381 acagcgccac gaagtcacgg ttccactcgt gcacccggcg cagatccgcg tgcccgccgg
     1441 cggtgagggc gcgcagcagg ttgagcgtcg ctgcggacgc ctggtagaca cgctccagcc
     1501 gggccgggtc aggggtgcgg gcctcggcgg tgaactccag cccgttgacg gcatcgcccc
     1561 ggtacgaggg cagcgtcacg ccgccccggg tctcggtggg ctgtgagcgg ggcttggcgt
     1621 actggccggc gatacggccg accttgacca ccgggacgcc ggccgcgtac gtcagcacgg
     1681 cggccatctg gaggaggacc tccagcttgt tgcggaccga ctccgcgccc accccgtcca
     1741 gggtctccgc gcagtcgccg ccctggagca ggaagccccg ccccagggct acttcgccga
     1801 gccggtcgag cagggtgtcg cactcctggc cgaacaccag cggcggtgcg gcggacaggg
     1861 ccgacaaagt ggtccgcagc gccgtgggat cgggccagtc gggctgctgc gccacgggca
     1921 gcgcccgcca cgcttcgaca gagggatcgg caagtgagtt ctcgtccatc tccagggatt
     1981 ccacgctgaa aacctcaacg ccttctgttc cgagggcagg gcacggggtt gcggtgcgcg
     2041 cagcctgtca cgcgccggcc gggccccggc agtgggacgt tgtggctaga agggcggatc
     2101 tactacccgg gtactagcgc cagcggccga ctgtcggggc cggccgcact cctggaccat
     2161 ttcggcaggc gccctcgtcg tcggcacccg acggcgggcg cggttcgccg catgtctcca
     2221 cgccaccggc tcgaaaggtg atcccgcatg agcagcaccg tcaacccgtc cgggtccgtg
     2281 tcccgcccgc agaccgggga cgagtacctg gagagcctcc gggacggccg ggaagtcagg
     2341 atctacggcg agaaggtgca ggacgtcacg gctcacccgg ccttccgcaa caactcccgc
     2401 atggtggccc ggctcttcga cgcgctccac gaccccgagc gcaacagcaa gctggtcgtc
     2461 cccaccgaca ccggcaacgg cggtttcacc cacccgtact tcaaggcccc gtacacctcc
     2521 ggcgacctgc ggaccgccgc gcgggccatg gaggagtggg cgcgcatgac gtacggctgg
     2581 ctgggccgca cccccgactt caaggccgcc ttcctgtcca ccctgggctc gaacaaggag
     2641 cactacgcgc cgttcgagga caacgcgtcc gcgtggtacc gcaaggcaca ggagaacgtg
     2701 ctcttcatcg gccacggcat cgtcaacccg ccgatcgacc gcaaccgggg gctgaccgag
     2761 ctcaaggacg tgatgctgac ggtggaccgg gagaccgacg ccggcctcgt ggtctccggc
     2821 gccaaggtcg tcggcaccgg ggccgcgctc acccagcaca tcttcatcgg cagctacggg
     2881 gtcataccga agggcgcgaa ggagttctcc gcgtacttca tcgtgccgac cgacagcccc
     2941 ggcctgaaga tcatctcccg gccgtcgtac gagttcgcct cggccaccgt cagcagcccc
     3001 ttcgaccagc cgctcagcag ccgcctggac gagaacgacg gcattctcgt cttcgaccag
     3061 gtgctgatcc cctgggagaa cgtcttctgc tacgacgtgg acaaggcgaa cgacttcttc
     3121 tacgggtcag gcttcttcta ccgggccatg ttccacgcct gcgtccgctt cgccgtgaag
     3181 atggacttcc tgaccggcct gctcgccaag tgcctggaga ccacgggcac ctcggagttc
     3241 cgcggggtgc agagccggct cggcgaggtc ctcgcctacc gcaacatgtt ctgggccctg
     3301 gtcgactcca tggccgagaa ccccaccaag tggcccgacg gcaccgtcac ccccaacggc
     3361 gagaccgcgc tggccttccg cgtcctgtcc tcgtcgatct tccccaaggt gcgtgagatc
     3421 ttcctgcggg atctcggcag cgcgctgatc tacaacaact cccacgccct cgactggtcc
     3481 aaccccgagg tccggcccta cctggacaag tacgtccgcg gcctgggcgt ggaggccatc
     3541 gaccgggtga agctgatgaa gctggcatgg gacgcggtcg gcaccgagtt cggggcccgc
     3601 tgggagctgt acgagatgaa ctacgcgggc aaccacgagc agatccggtt cgaggcgctg
     3661 cacgcccagc aggtctccgg gaagttcgac gactacaagc agctcgtcga ccggtgcctg
     3721 tcggagtacg acgagcacgg ctggaaggtc cccgacctcg tcgaccccaa ggacaccacc
     3781 gtcatcggcg ccggcgcacc ggacaccggc acgaccgccc cccgcccggc cgcacggtga
     3841 gcagccacac ccccgccccg ggcggaccca ccgccccgcc cggagccccc gtcctgccgg
     3901 accaggccca actcaggcac gtactcggcc agttcgtgac cggggtgtgc gcggtgtcga
     3961 cccgcttcga cacccgctcc ggcgtacgcc acgacgccat cgcggtgaac tcgttcacct
     4021 cggtctccct cgacccgccg ctcgtgtcga tgtacctgcg cgacgactcc accttcctgc
     4081 ggcgggtccg cacgtccggc cagtgggccg tgtcgttcct cggcaaccgg gcgcgctccc
     4141 tggtcggcgt cctcaccgcg cccgcggccc ggcgccctcc ggtggagcag gccgccgact
     4201 gggccgtcgg cccgcacacc ggctgtctgg tcctgccgga cggccccgga acgctcgaat
     4261 gcgctctgca cctcacgcag gcactcggcg accatgtcct cgtggtcgga cgggtgctgg
     4321 gcgcggtggg ccgcgaccac gaacccctgg tcttccaccg cggcgccttc accgaagtcg
     4381 caccggcgcc gtgaccacgc tccaccggcc ttcggcagcg aacaggaaag gagaccccat
     4441 ggcgaggaaa cgcccgtacg tggcggtggt gggcggcggc atcggcggcc tggccgtcgc
     4501 gctgggactg cgcaggcagg gcgtcgaggc ggtcgtccac gagcaggcgc acgcactgtc
     4561 ccaccagggg gcggggatcg cgatcggcgc gaacggacac cgggccctgc gggaactggg
     4621 cgtcgccaag cggctgaccg cctctgccgc ccggccgtcg cgcgccgact tccggcactg
     4681 gcgcaccggc cggtccatgg tgtcgcaccg cctgaccggc ctgtacgagg aacgcttcgg
     4741 cgcccccttc tggaccgtgg aacgcgcggc cgtccagcag gctctgctcg ccgaactggg
     4801 cccgcgccac gtgcgcctgg gcgcgcgctg caccggggtg gacaggacgg ccgacggcgc
     4861 cgtgatccgc ttcgaggacg gcggcgaggc ggaggccgac gcggtcgtcg gggcggacgg
     4921 tatccactcc gcggtgcgcc acagcctctt cggcccgcag gaggccgtgt tctccggcac
     4981 cagcggatac cgcgccctgg tcccgatgga ccggctgcgg cacgttcccg aactcgccga
     5041 accggtgctg tggctgtggc tcggaccggg ccggcacttc atcgcctacc cggtggccga
     5101 cggcagcgcg ctgaacttcc tggccgtcgt gcccgaccgc acgtggacgg tggagtcctg
     5161 gtccacggag ggcgacgctg ccgagctgcg ggccgccttc gacggctggc acccgttcgt
     5221 cacggaggtc ctcggcgcct gcgaacgtcc gggccgctgg gcgctgtacg accgggagcc
     5281 gcagcgcgtg tggagttccg gcgcggtgac gctgctggga gatgccgcgc acgcgatgct
     5341 gccgcaccac ggacagggcg ccaaccaggc cctggaggac gcggtggtcc tcgcccactt
     5401 cctggcacgg acggacaccg gcggtgtgcc gtcggccctg cgcgcctacg agcgtctgcg
     5461 tcggccgcgc acccggctgc tccaggcggg ctcccgtaag aacgccggct gcttccagct
     5521 ccccgacggg ccgcaggccg aagcccgcaa cgcaaggctg gccaccctgc ccgacgacgt
     5581 cgcctggatc cacgggcacg acatcctcgg ctccctgcct gtggccacgt caccggcctg
     5641 aaccgcgggc gggggccgcc gaccgcgacg accatcgacg aacggtgagg aacaccatgg
     5701 caaggaccta ctccttcgaa ggcaacgtac ccgtcgtgca tcccacggcg ttcgtgcacc
     5761 ccgacgccgt gctgatcggg tcggtcgaca tcggtccggg gtgctatgtc ggccccctcg
     5821 cgagcctgcg gggcgacttc gggcacatcg aactccgggc gggctccaac gtccaggacg
     5881 gctgcgtcct gcactgcttc ccgggggccg acacggtcgt ggaggaggac ggacacgtcg
     5941 gccacggcag cgtgctgcac ggctgccggg tgggccggga cagcctcatc ggcatgaagt
     6001 ccgtcctcat ggacggtgtc gtggtgggaa cgcgggcgtt cgtcggggcc ggcagcttcg
     6061 tcaagtcccg cttccaggta ccggagcggc atctggtggc gggcagcccg gccaaggtgg
     6121 tgcgtgaact caccgccgac gagatcgcct ggaagggcaa cgggaccgcc cagtaccaga
     6181 agctggccca gcgctgtctc accggtctgc acgcggcgga ggccgccacc gagcgcaccg
     6241 ccccggcggc ccccgccgaa cccggcgagc acgaacacgt caccctccac gcctaccggt
     6301 cccgctgacg aagcacccga ggcaccttgt cgcggccgat ccgcgcaccg gacaagaccg
     6361 tcaggacggg ccccgcgacg gcgccgggct cgcgcgaacg ccaggcccat tcaccggacg
     6421 tcacgttcag ggacgtcacg ttcacggacg tcatgttcaa ggacttcacg ctcaaggacc
     6481 ttccgcggct ccccagggcc gcgacccttc cccaccccct ccaggaggca tgttcatgaa
     6541 cagctcagcc acctcccgac tcgtcgtcgg cgaccggttc cgcgacttcg ccgaggccgt
     6601 cggcgccgag acgttctcgc ggctcgcccg ctcgctcgac gcgggcgacc tggacgcggt
     6661 cgagggaccg ctgcgcgtgg tcaccggcca gggcgtcggc gagttcgaga tctcctacct
     6721 cgaggacgcc gtccgccggc ggcagctgga cgaccggatc gagctggtcc actcggccgg
     6781 gcagcccgcc cgtcgccagg aactgcacaa ggcccgcgag gacaacgtgc tgatcgccgg
     6841 gctcacacag gtcgacgaca ccgcctacga ggccgccctg aggctgcacg accacaacga
     6901 actgctcgtc gacgtccagg accgggtgca cgtgcagggc atggtggccg tcgaggcggc
     6961 ccgtcagatg ttcgtcgcgg tggtggagcg ctacttcacg gcccgctggc cgcagcagcg
     7021 ctactacatc gtcctcaact ccatcaacac cacgttctcc aacttcctct tccccgtggg
     7081 tgccacggtg cggctgacgg tggacgagtc ggaggtgtcg gagcccagcc ggctcacgtt
     7141 ccgtacgacg gtggaggtgg agcaggccgg acggcccgcc gctgccgtca ccatcgacgc
     7201 ggccgccttc gcccccgggc tgctggagga gaaggagctg cgccgggcca agagcgcggt
     7261 ggcgagcacg atggacgccg cactggccat cgcgtgacat gaggacgccg acaccggaca
     7321 cccggacgcg ccccgcaccg ggacgactcc cgtacccgga gtggcggttc gcctacctgg
     7381 gcccggaagg aaccttcacc gagctggccc tgcggtccct cccggaggcc acgggcgccc
     7441 ggctgtcacc ggctgccacg gccaccgagg ccctgacgct ggtgtgccgg ggcgccgcgg
     7501 acgccgccat gctgcccgtc cacaacacgg tggccggggt ggtcgcggac acggtgcgcg
     7561 cgctcgccga gtcacccgcg ctgaccgtcc tcagggaagt cgtgctcccc gtggaattcg
     7621 cccttctggt gcgccccggc acacgacgcg cgcggatccg cacggtgagc ggccatccgc
     7681 acgcgggagc gcaggtcggc ggatggctcg acagccgggc gccgcaggcg cgttggacac
     7741 cggcgccgtc caacgccgag gccgcgcgca gggtacggga ccgggagtgc gacgccgcgg
     7801 tcgccgggga gttcaccgcc gcgcactacg gccttcaggt cctggacgcc gggatacagg
     7861 acaccgccgg tgcggtcacc cggttcctgc tgtgcgcgcg tcccggcagg tggagcggcc
     7921 cgcgtcccgc cgccgaccgc acctcgctgg tcgggcgtct cgcggcccgg cccgaggagg
     7981 ccggcgggct gctcgacggc ctgcggagcc accccttcct gcggtcggtg tccctgcgga
     8041 ccgtgtccga cggccgcagc ggctcggtcc tgttcgccga ctgcccggga ggcgccggac
     8101 aggccgcgac agcgcgcgcg gtcggcctgc tgcgcctgcg gctgcccgga ctgagagtcc
     8161 tggggccgta cccctcggcg acccatgtcc cgtcgtacga acggagcgag accatgcaca
     8221 ccgagaccgc cgccgaagcc ggcctctcag cggagaccgg gtcccgggac atcgcccggc
     8281 tgcgcgaccg gatcgactcc ctggaccacc tgctcatcgg cacgctcagg gagcggctgg
     8341 aggcgtcccg cgagatccag cggatccgta cccgaagcgg cggctcccag gtcgaccccg
     8401 ggcgcgaggc cgccgtccgg ggccggtacg ccgaggaact gggggagggc ggaacgggcg
     8461 tcgccgaggc gctgctggac atgtgccggg ggcggtcacc acggtgaacc cgcccccgac
     8521 gacgacggcg acgtcgtcac cccgcccccg tcgcggccgt gaggacgtcc tcacggccgc
     8581 gacggccttg ttccacgagc ggggttacga cgcgacgagc atgagcgaca tcgccgcacg
     8641 cctcggcttc accaaggcgg cgctgtaccg gcacgtgacg ggcaaggccg aactgctgcg
     8701 ggcgatcacg cggccggtcc gcgcggacgt gcgggccctg ctggccgcga gcaccgccgg
     8761 agacgaccgg cccgtcgacc aactctcgga gctgttacga gggttggccg gcgccgcggc
     8821 ggccgacccg ggccggtacg tcctcttctg ggggccggac ggcgagcgga gcccgcacgg
     8881 gcccgacgcc gtgtgccgtg cggcggtggt ccagcggctg acggaactgc tggagcgcgc
     8941 cgccgaccgc ggcgagatac gcgacggcat cgttccccac ctggccgccc ggctgctcgt
     9001 cggcgcggtc accggtccgg gccggccggt gtcaccgtcg gccgtggacg tcctgctcga
     9061 cgggccggtg cggcgtctga acgacggggc aggccggggg cgggggcgct ga
//
