BGC0001495 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001495 (Actinoallomurus fulvus)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	56,721	High	actinoallolide A	PKS

BGC0001495 - Region 1 - T1PKS

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Location: 1 - 56,721 nt. (total: 56,721 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 56,722
Label		BGC0001495

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

aalA1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

aalB: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

aalC: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

aalD: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Ligases: synthase

aalA2: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

aalA3: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001495.3	1.00	PKS	actinoallolide A	Actinoallomurus fulvus
BGC0000041.3	0.72	PKS	oronofacic acid	Pseudomonas syringae
BGC0000067.5	0.64	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001051.5	0.61	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0001012.3	0.59	NRPS, PKS	meridamycin	Streptomyces violaceusniger
BGC0001665.3	0.58	PKS	methylated alkyl-resorcinol, methylated acyl-phloroglucinol	Mycobacterium marinum M
BGC0000018.5	0.57	PKS	angolamycin	Streptomyces eurythermus
BGC0000024.5	0.56	PKS	aureothin	Streptomyces thioluteus
BGC0001065.5	0.56	PKS	herboxidiene	Streptomyces chromofuscus
BGC0000147.5	0.55	PKS	soraphen A	Sorangium cellulosum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001495.3	1.00	PKS	actinoallolide A	Actinoallomurus fulvus
BGC0000041.3	0.72	PKS	oronofacic acid	Pseudomonas syringae
BGC0000067.5	0.64	PKS	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0001051.5	0.61	NRPS, PKS	thuggacin A	Sorangium cellulosum
BGC0001012.3	0.59	NRPS, PKS	meridamycin	Streptomyces violaceusniger
BGC0001665.3	0.58	PKS	methylated alkyl-resorcinol, methylated acyl-phloroglucinol	Mycobacterium marinum M
BGC0000018.5	0.57	PKS	angolamycin	Streptomyces eurythermus
BGC0000024.5	0.56	PKS	aureothin	Streptomyces thioluteus
BGC0001065.5	0.56	PKS	herboxidiene	Streptomyces chromofuscus
BGC0000147.5	0.55	PKS	soraphen A	Sorangium cellulosum

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 56721:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ohmal - Me-ohmal - Me-ccmal) + (Me-redmal - pk - ohmal - Me-ccmal) + (Me-redmal - pk - mal)
Non-colinear ordering:: aalA1 + aalA2 + aalA3

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

aalA1: mmal - mmal - mmal - mmal

PKS_AT (506..786): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 199.5
Ethylmalonyl-CoA: 189.4
Methoxymalonyl-CoA: 161.9
2-Methylbutyryl-CoA: 147.2
trans-1,2-CPDA: 133.2
Propionyl-CoA: 109.7
CHC-CoA: 96.8
Malonyl-CoA: 88.8
Isobutyryl-CoA: 71.6
Benzoyl-CoA: 70.9
Acetyl-CoA: 44.3
3-Methylbutyryl-CoA: 40.7
fatty_acid: 34.9
inactive: 24.6

PKS_AT (1447..1731): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 87.5%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 211.6
Ethylmalonyl-CoA: 198.7
Methoxymalonyl-CoA: 173.4
2-Methylbutyryl-CoA: 145.0
trans-1,2-CPDA: 132.0
Propionyl-CoA: 112.3
Malonyl-CoA: 107.7
CHC-CoA: 103.2
Isobutyryl-CoA: 77.3
Benzoyl-CoA: 75.4
Acetyl-CoA: 48.6
inactive: 40.6
3-Methylbutyryl-CoA: 37.8
fatty_acid: 35.5

PKS_AT (2832..3111): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 204.7
Ethylmalonyl-CoA: 187.1
Methoxymalonyl-CoA: 167.5
2-Methylbutyryl-CoA: 162.1
trans-1,2-CPDA: 129.9
Propionyl-CoA: 118.5
CHC-CoA: 98.7
Malonyl-CoA: 93.8
Benzoyl-CoA: 74.3
Isobutyryl-CoA: 70.9
3-Methylbutyryl-CoA: 47.5
Acetyl-CoA: 43.0
fatty_acid: 32.3
inactive: 29.3

PKS_AT (4229..4509): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 203.2
Ethylmalonyl-CoA: 189.1
Methoxymalonyl-CoA: 164.9
2-Methylbutyryl-CoA: 151.8
trans-1,2-CPDA: 127.0
Propionyl-CoA: 113.3
CHC-CoA: 97.0
Malonyl-CoA: 93.8
Benzoyl-CoA: 71.4
Isobutyryl-CoA: 68.8
3-Methylbutyryl-CoA: 47.2
Acetyl-CoA: 45.9
fatty_acid: 36.1
inactive: 27.1

aalA2: mmal - pk - mal - mmal

PKS_AT (538..817): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 199.2
Ethylmalonyl-CoA: 179.4
Methoxymalonyl-CoA: 165.2
2-Methylbutyryl-CoA: 146.2
trans-1,2-CPDA: 128.3
Propionyl-CoA: 111.5
Malonyl-CoA: 94.2
CHC-CoA: 85.5
Benzoyl-CoA: 77.7
Isobutyryl-CoA: 73.6
Acetyl-CoA: 46.5
3-Methylbutyryl-CoA: 42.4
fatty_acid: 31.9
inactive: 30.2

PKS_AT (2524..2809): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 209.6
Methylmalonyl-CoA: 207.9
Methoxymalonyl-CoA: 172.4
2-Methylbutyryl-CoA: 142.4
trans-1,2-CPDA: 126.2
Propionyl-CoA: 107.9
CHC-CoA: 107.7
Malonyl-CoA: 103.5
Isobutyryl-CoA: 75.7
Benzoyl-CoA: 74.4
Acetyl-CoA: 49.8
fatty_acid: 39.5
3-Methylbutyryl-CoA: 33.3
inactive: 24.4

PKS_AT (3888..4174): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 206.3
inactive: 93.1
Methoxymalonyl-CoA: 64.9
Methylmalonyl-CoA: 60.8
Propionyl-CoA: 34.5
2-Methylbutyryl-CoA: 27.9
Ethylmalonyl-CoA: 25.9
CHC-CoA: 17.9
Isobutyryl-CoA: 16.2
Benzoyl-CoA: 15.3
Acetyl-CoA: 15.0
fatty_acid: 14.3
trans-1,2-CPDA: 12.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (5293..5575): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.9
Ethylmalonyl-CoA: 205.1
Methoxymalonyl-CoA: 183.6
2-Methylbutyryl-CoA: 145.6
trans-1,2-CPDA: 128.3
Propionyl-CoA: 116.4
CHC-CoA: 108.6
Malonyl-CoA: 97.2
Isobutyryl-CoA: 82.5
Benzoyl-CoA: 74.9
Acetyl-CoA: 44.4
3-Methylbutyryl-CoA: 42.7
fatty_acid: 36.1
inactive: 28.2

aalA3: mmal - pk - mal

PKS_AT (562..844): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 214.5
Ethylmalonyl-CoA: 205.9
Methoxymalonyl-CoA: 173.8
2-Methylbutyryl-CoA: 135.8
trans-1,2-CPDA: 121.0
CHC-CoA: 117.3
Propionyl-CoA: 112.1
Malonyl-CoA: 100.0
Isobutyryl-CoA: 91.7
Benzoyl-CoA: 86.5
Acetyl-CoA: 62.8
3-Methylbutyryl-CoA: 59.8
fatty_acid: 31.1
inactive: 24.9

PKS_AT (2546..2844): pk

ATSignature: Methoxymalonyl-CoA

Top 3 matches:: Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%; Methylmalonyl-CoA: 79.2%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 184.8
Methylmalonyl-CoA: 184.2
Methoxymalonyl-CoA: 142.6
trans-1,2-CPDA: 106.7
2-Methylbutyryl-CoA: 102.1
Isobutyryl-CoA: 94.6
Malonyl-CoA: 87.9
Propionyl-CoA: 82.4
CHC-CoA: 73.8
Benzoyl-CoA: 55.5
3-Methylbutyryl-CoA: 49.7
fatty_acid: 37.2
Acetyl-CoA: 27.1
inactive: 13.9

PKS_AT (3550..3845): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.5
inactive: 118.5
Methoxymalonyl-CoA: 62.9
Methylmalonyl-CoA: 43.3
Ethylmalonyl-CoA: 26.1
Propionyl-CoA: 23.9
Isobutyryl-CoA: 23.9
Benzoyl-CoA: 19.0
2-Methylbutyryl-CoA: 18.1
fatty_acid: 17.3
Acetyl-CoA: 16.6
CHC-CoA: 9.8
trans-1,2-CPDA: 8.5
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor