LOCUS       BGC0001507              3506 bp    DNA     linear   BCT 27-MAY-2014
DEFINITION  Streptomyces olindensis strain DAUFPE 5622 103, whole genome shotgun
            sequence.
ACCESSION   BGC0001507
VERSION     BGC0001507
KEYWORDS    .
SOURCE      Streptomyces olindensis
  ORGANISM  Streptomyces olindensis
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 02:09:21
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3506
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[385:2884]"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      386..2884
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     cand_cluster    1..3506
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..3506
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001507"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..3506
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             163..351
                     /gene="allorf_0163_0351"
                     /gene_functions="biosynthetic-additional (lassopeptides)
                     predicted lassopeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="allorf_0163_0351"
                     /protein_id="allorf_0163_0351"
                     /tool="antismash"
                     /transl_table=1
                     /translation="VQRVAARLPFVTVEDAMDEQTEFSTTEPYAPPTLTEVGEFNEDTL
                     GFIGWGDDIFGHYSGDF"
     CDS_motif       163..297
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence:
                     VQRVAARLPFVTVEDAMDEQTEFSTTEPYAPPTLTEVGEFNEDTL"
                     /locus_tag="allorf_0163_0351_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       298..351
                     /prepeptide="core"
                     /locus_tag="allorf_0163_0351_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="3.30"
                     /molecular_weight="1872.0"
                     /monoisotopic_mass="1870.8"
                     /core_sequence="GFIGWGDDIFGHYSGDF"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="VQRVAARLPFVTVEDAMDEQTEFSTTEPYAPPTLTEVGEFN
                     EDTL"
                     /leader_location="[162:297](+)"
     gene            386..2221
                     /locus_tag="DF19_05060"
     CDS             386..2221
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 101.2; E-value: 9.5e-31)"
                     /gene_kind="biosynthetic"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_018090023.1"
                     /locus_tag="DF19_05060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="asparagine synthetase"
                     /protein_id="KDN73296.1"
                     /sec_met_domain="Asn_synthase (E-value: 4.1e-38, bitscore:
                     119.9, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSTGFVVLPDTAAAADARAAVPWASPREFAHASGRPWLVGRWAP
                     GDITVVTAGPVRVAVAGVCPVPAARLTRLAARVRTVSDADELAAELPGSCHLVVSAHGD
                     VRFQGSATGLRRVFRTRTHGVEIAADRADVLAAMTGAGIDERALALRVATGGFLPPPLG
                     DHTLWSGITPLAPNHCLMWERDRLRETRWWQPPAPELSLRAGAEKLRDTLATALGDRAP
                     GQGRLSSDLSGGMDSTSLCFLAARDTPDLLTFRWGEAEAGNDDAAFAAHAMGSLDRAEH
                     LVVPPGDLPPLFTDADAPVDTEEPYPFVRTLARTRHTARLLSGSGSRRHLAGHGGDELF
                     TPFPGYLHALLRRRPLTALRHVRGYGALKRWPLLPTLAALTRPGDEADWWRAQADRLTD
                     PSPPKRRPALGWGLWTLRAPSWATGDAIDAAREALRDTAAHVRPLSADLAQHQSLLAVR
                     TSAPWYRLLARVFADDGVQLDSPFFDDRVVEAVLAVRAHEHAGPWRYKPLLAEAMRGIV
                     PEPVLGRSTKGEFSDEANSGLRRNLPAVLELFEDSALAAHGLIDPGELRTRLLAPQPDN
                     STRIALEPLIGCETWLRSATRPAPAWRFDARASAS"
     gene            2205..2456
                     /locus_tag="DF19_05065"
     CDS             2205..2456
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: PqqD_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Lasso_Fused_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_018090022.1"
                     /locus_tag="DF19_05065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="KDN73297.1"
                     /sec_met_domain="PF05402 (E-value: 3.5e-19, bitscore: 57.1,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1.2e-28,
                     bitscore: 87.2, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 1.8e-10,
                     bitscore: 29.0, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="PqqD_RRE (E-value: 2e-09, bitscore: 25.3,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLHPDVSTTDTDDGTVLLNERTGRYWQLNNTGAHVLRRLLGGQE
                     PAGIAADLANRYGIAPEQAERDVAAVIERLRGSELVIS"
     gene            2453..2884
                     /locus_tag="DF19_05070"
     CDS             2453..2884
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_018090021.1"
                     /locus_tag="DF19_05070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="KDN73298.1"
                     /sec_met_domain="PF13471 (E-value: 1e-29, bitscore: 91.2,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSMSVALAERRRLPPHRRVLPLLVVGAARLLARAKPARLRTVLEF
                     ARRGAAPATAAQALRAREEVVSVSLRCAGQGCLQRSIATALLCRVRGVWPTWCTGVRTH
                     PFAAHAWVEAEDQLVGEGHPKGHFKTLMSIPPVPDERNR"
     gene            2964..3506
                     /locus_tag="DF19_05075"
     CDS             2964..3506
                     /codon_start=1
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_010046512.1"
                     /locus_tag="DF19_05075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="membrane protein"
                     /protein_id="KDN73299.1"
                     /transl_table=11
                     /translation="MTTTLIWIAVALVAVGLYLSWTAGRLDRLHARIDATRAALDAQLL
                     RRASVAQELATSGVLDPAASIVLYEAAHAARQAEEEQREVAESELSQALRAVFGEAQQV
                     EAVREAPGGEEAAEELAQAVRRVPMARRFHNDAVRAARALRRHRKVRWFRLAGHAPFPM
                     AFEMDDEPPEALAARAA"
ORIGIN
        1 ttccggccac gcggaagagg ccttcgcaca ggtctccgtc cggccgtccc atgatcgatc
       61 ccgctgatca caggcgatct tgacctctcg ctcgcgtgtg cgaagtgacc cctgtgactt
      121 gagtgaaaat ccgatggatc atggccgctg cccgaatgta cggtgcaacg ggtagccgcc
      181 cggctgccct tcgtaaccgt ggaggatgcc atggacgagc agaccgagtt ctcgaccacc
      241 gagccgtacg cgccgccgac gctgaccgag gtcggcgagt tcaacgagga caccctcggc
      301 ttcatcggct ggggcgacga catcttcggc cactactccg gcgacttctg atcgtcggcg
      361 cgtcggctcc cggagacatg agcagatgag cagcaccggg ttcgtggtcc tgccggacac
      421 ggcagccgcg gccgatgcgc gcgcggcggt gccctgggcg tcgccgcggg agttcgcgca
      481 cgcctcggga cgaccgtggc tggtgggccg gtgggccccc ggcgacatca cggtcgtcac
      541 ggcgggcccg gtgcgggtgg cggtggccgg ggtctgcccg gtccccgccg cccggctcac
      601 ccgactcgcc gcccgcgtcc ggaccgtgtc cgacgcggac gaactcgcgg cggagctgcc
      661 cggctcctgc cacctggtgg tctcggccca cggagacgtg cgcttccagg gcagcgcgac
      721 cggactccgc cgggtcttcc gcacccggac acacggggtc gagatcgcgg cggaccgcgc
      781 cgacgtgctc gccgcgatga cgggcgccgg catcgacgaa cgggcgttgg ccctccgcgt
      841 ggccacgggc gggttcctcc cgcccccgct cggcgaccac accctgtggt ccgggatcac
      901 cccgctggcg ccgaaccact gcctcatgtg ggagcgcgac cggctgcgcg agacgcgctg
      961 gtggcagcca cccgctcccg aactgtccct gcgggcaggc gccgagaagc tccgggacac
     1021 cctcgccacg gccctcggcg accgtgcccc tggccagggc cggctgagct ccgacctctc
     1081 cggcggcatg gactccacgt ccctgtgctt cctcgccgcc cgggacaccc ccgacctgct
     1141 gacgttccgc tggggagagg ccgaggcggg caacgacgat gccgccttcg ccgcccacgc
     1201 catgggctcc ctcgaccggg ccgagcacct cgtggtgccc cccggcgacc tcccgcccct
     1261 gttcaccgac gcggacgcac cggtggacac cgaggagccc tatccgttcg tccgcaccct
     1321 cgcccggacc cgccacaccg cccggctgct gtccggcagc ggatcgcgcc ggcacctcgc
     1381 cggacacggc ggcgacgaac tgttcacccc gttccccggc tacctccacg ccctgctccg
     1441 gcgccgtccg ctgaccgccc tgcgccacgt ccgcggatac ggcgcgctga agcgctggcc
     1501 cctgctgccc accctcgccg cgctgacccg ccccggggac gaggccgact ggtggcgagc
     1561 ccaggccgac cggctcaccg acccgtcccc gccgaaacgc cgccccgccc tcgggtgggg
     1621 gctgtggacg ctgcgagcac cgtcgtgggc caccggcgac gcgatcgacg ccgcccggga
     1681 ggccctgcgg gacaccgcgg cccacgtgcg gccgctctcc gccgacctgg cccagcacca
     1741 gtcactgctc gccgtgcgca ccagcgcccc gtggtaccgg ctgctggccc gcgtcttcgc
     1801 ggacgacggc gtccagctcg actccccgtt cttcgacgac cgcgtggtgg aagcggtcct
     1861 cgcggtccgg gcccacgaac acgccggccc ctggcggtac aagcccctgc tcgccgaggc
     1921 gatgcgcggc atcgtgccgg agccggtgct gggccgctcc accaagggcg agttcagcga
     1981 cgaggccaac agcggactgc gccgcaacct gcccgccgtc ctggagctgt tcgaggactc
     2041 cgccctggcc gcccacggcc tgatcgaccc cggtgaactg cgcacccggc tgctcgcgcc
     2101 ccagccggac aactccacgc ggatcgccct ggagcccctg atcggctgcg agacctggct
     2161 gcgctccgcc acccgccccg cccctgcttg gaggttcgac gcccgtgcgt ctgcatcctg
     2221 acgtgagcac caccgacacc gacgacggca cggtcctgct caacgaacgc accggccggt
     2281 actggcagtt gaacaacacc ggagcccatg tgctgcgccg gctgctcggc ggacaggagc
     2341 ccgccggcat cgccgccgac ctcgcgaacc ggtacggcat cgcccccgag caggccgaac
     2401 gcgatgtggc cgccgtgatc gagcggctgc gcggctcgga actggtgatc tcatgagcat
     2461 gtccgtcgcg ctggccgaac ggcgccgcct ccccccgcac cgccgcgtgc tgcccctgct
     2521 ggtcgtcggt gccgcccggc tgctggccag ggccaaaccc gcccggctgc gcaccgtcct
     2581 ggagttcgcc cgccgcggcg cggcaccggc cacggccgcc caggcgctcc gggcccgcga
     2641 ggaggtcgtg tccgtcagcc tgcgctgcgc cgggcagggc tgcctgcaac gctccatcgc
     2701 caccgccctg ctctgccggg tgcgcggcgt ctggcccacc tggtgcaccg gagtgcgcac
     2761 ccacccgttc gccgcccacg cctgggtcga ggccgaggac cagctcgtcg gcgagggaca
     2821 ccccaagggg cacttcaaga cactgatgtc catcccgccg gtgccggacg aacggaaccg
     2881 gtgaccgatc caggcagcac ggggcaggac agggcagggc ggggtgaacc gacacctccc
     2941 tcctgccgct agccttgccg cccgtgacca ccactctcat ctggatcgcc gtggccctgg
     3001 tcgcggtcgg gctctatctg agctggaccg ccggccgcct cgaccgcctc cacgcccgga
     3061 tcgacgccac ccgggccgcc ctggacgcgc agttgctgcg ccgggcctcg gtggcgcagg
     3121 aactggcgac gtcgggggta ctggatccgg ccgcctcgat cgtgctgtac gaggccgcgc
     3181 acgccgcccg gcaggcggaa gaggagcagc gggaggtcgc cgagagcgaa ctgagccagg
     3241 cgctgcgggc cgtcttcggg gaggcgcagc aggtggaggc ggtgcgcgag gcgcccgggg
     3301 gtgaggaggc cgccgaggaa ctcgcgcagg cggtgcggcg cgtccccatg gcccgtcgct
     3361 tccacaacga cgccgtacga gccgcgcgtg ccctgcgccg ccaccgcaag gtccgctggt
     3421 tccgcctggc gggccacgcc ccgttcccga tggccttcga gatggacgac gagccaccgg
     3481 aggccctcgc ggcccgggcc gcctga
//
