BGC0001519 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001519 (Streptomyces aurantiacus JA 4570)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	69,225	High	aurantimycin A	NRPS:Type I+PKS

BGC0001519 - Region 1 - NRPS,T1PKS

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Location: 1 - 69,225 nt. (total: 69,225 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

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transport-related genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 69,226
Label		BGC0001519

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

STRAU_0322: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

STRAU_0323: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

STRAU_0324: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

STRAU_0342: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

STRAU_0346: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase halogenase

STRAU_0334: halogenase

smcogs: SMCOG1119: halogenase

STRAU_0347: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

STRAU_0338: thioesterase

smcogs: SMCOG1004: thioesterase

STRAU_0343: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

STRAU_0325: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

STRAU_0328: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

STRAU_0332: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

STRAU_0335: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

STRAU_0336: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

STRAU_0337: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

STRAU_0344: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

STRAU_0345: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

STRAU_0349: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001519.5	1.81	NRPS, PKS	aurantimycin A	Streptomyces aurantiacus JA 4570
BGC0001276.3	1.39	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002075.3	1.20	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	1.17	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	1.16	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	1.15	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	1.13	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	1.12	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	1.08	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001261.3	1.06	NRPS	AM-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001519.5	0.99	NRPS, PKS	aurantimycin A	Streptomyces aurantiacus JA 4570
BGC0001276.3	0.77	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002075.3	0.76	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 69225:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - X) + (D-Gly - Leu) + (Ala) + (Me-mal) + (ccemal) + (redmal) + (Me-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 53221:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - X) + (D-Gly - Leu) + (Ala) + (Me-mal) + (ccemal) + (redmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 19329 - 69225:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X - X) + (D-Gly - Leu) + (Ala) + (Me-mal) + (ccemal) + (redmal) + (Me-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 38136 - 69225:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-mal) + (ccemal) + (redmal) + (Me-mal) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

STRAU_0332: X

AMP-binding (19..425): X

nrpys: Leu, Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLRHLGQDVK
Nearest Stachelhaus code(s):: Leu or Ser DLRHLGQDVK (100% 8Å match); Leu or Ala DLRHLGQDVK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

STRAU_0335: X - X

AMP-binding (468..865): X

nrpys: Piz

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVFSVASYAK
Nearest Stachelhaus code(s):: Piz DVFSVASYAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2019..2400): X

nrpys: Ala, Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLVDK
Nearest Stachelhaus code(s):: Ala or Ser DVWHFSLVDK (100% 8Å match); Ser DVWHFSLVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

STRAU_0336: Gly - Leu

Search NORINE for peptide: strict or relaxed

AMP-binding (466..842): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code(s):: Gly DILQLGVIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1998..2386): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVF-VAAYAK
Nearest Stachelhaus code(s):: Leu DVFSVAAYAK (88% 8Å match)
Stachelhaus code match:: 90% (moderate)

STRAU_0337: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (468..844): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DMTQIGWTWK
Nearest Stachelhaus code(s):: Ala DMTQIGWTWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

STRAU_0344: mmal

PKS_AT (522..819): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Methoxymalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 188.5
Ethylmalonyl-CoA: 184.9
Methoxymalonyl-CoA: 152.7
2-Methylbutyryl-CoA: 121.5
Malonyl-CoA: 99.1
trans-1,2-CPDA: 96.4
CHC-CoA: 94.7
Propionyl-CoA: 87.9
Isobutyryl-CoA: 61.7
Acetyl-CoA: 46.9
Benzoyl-CoA: 45.1
inactive: 41.8
3-Methylbutyryl-CoA: 34.8
fatty_acid: 19.1

STRAU_0345: emal

PKS_AT (597..895): emal

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 91.7%; Methylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 200.2
Methylmalonyl-CoA: 190.8
Methoxymalonyl-CoA: 167.0
2-Methylbutyryl-CoA: 125.8
trans-1,2-CPDA: 124.8
Malonyl-CoA: 107.4
Propionyl-CoA: 102.4
CHC-CoA: 100.9
Isobutyryl-CoA: 77.3
Benzoyl-CoA: 53.2
Acetyl-CoA: 37.9
inactive: 34.3
fatty_acid: 30.4
3-Methylbutyryl-CoA: 28.6

STRAU_0346: mal

PKS_AT (578..874): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 66.7%; Ethylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 147.6
Methylmalonyl-CoA: 75.6
Methoxymalonyl-CoA: 63.2
inactive: 56.7
Ethylmalonyl-CoA: 47.2
CHC-CoA: 46.3
Propionyl-CoA: 35.7
2-Methylbutyryl-CoA: 31.3
fatty_acid: 29.2
Isobutyryl-CoA: 28.9
Acetyl-CoA: 28.9
trans-1,2-CPDA: 26.6
Benzoyl-CoA: 22.9
3-Methylbutyryl-CoA: 8.1

STRAU_0347: mmal

PKS_AT (575..870): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 222.0
Ethylmalonyl-CoA: 221.7
Methoxymalonyl-CoA: 185.6
2-Methylbutyryl-CoA: 171.0
trans-1,2-CPDA: 142.4
Propionyl-CoA: 125.4
Malonyl-CoA: 122.5
CHC-CoA: 109.2
Isobutyryl-CoA: 94.7
Benzoyl-CoA: 63.6
inactive: 50.0
Acetyl-CoA: 48.8
3-Methylbutyryl-CoA: 45.4
fatty_acid: 33.7

STRAU_0349: X

AMP-binding (462..862): X

nrpys: 3OH-Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALWWGGVLK
Nearest Stachelhaus code(s):: 3OH-Leu DALWWGGVLK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor