BGC0001523 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001523 (Streptomyces sp. 211726)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	129,796	High	azalomycin F3a	PKS

BGC0001523 - Region 1 - T1PKS

Download region GenBank file

Download region SVG

Location: 1 - 129,796 nt. (total: 129,796 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 129,797
Label		BGC0001523

Tailoring functions

Oxidoreductases: oxygenase

azl2: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acylase

azl5: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

azlE: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

azlG: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

azlH: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: dehalogenase

azl7: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

Lyases: hydratase

azl13: Nitrilase/cyanide hydratase and apolipoprotein

smcogs: SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein

Ligases: ligase synthase

azl4: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

azlA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

azlB: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

azlC: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

azlD: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

azlF: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Translocases: permease

azl14: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001523.5	1.00	PKS	azalomycin F3a	Streptomyces sp. 211726
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001700.5	0.76	PKS	niphimycin C, niphimycin D, niphimycin E, 17-O-methylniphimycin, niphimycin Iα, 19-O-malonylniphimycin	Streptomyces sp. IMB7-145
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.68	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001523.5	1.00	PKS	azalomycin F3a	Streptomyces sp. 211726
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001700.5	0.76	PKS	niphimycin C, niphimycin D, niphimycin E, 17-O-methylniphimycin, niphimycin Iα, 19-O-malonylniphimycin	Streptomyces sp. IMB7-145
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.68	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001258.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 129796:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - pk - ccmal) + (pk - ohmal) + (redmal) + (ohmal - ohmal - ohmal) + (ohmal - ohmal - mal - pk - redmal) + (pk - ohmal) + (pk - ccmal) + (Me-ccmal)
Non-colinear ordering:: azlB + azlC + azlA + azlD + azlE + azlF + azlG + azlH

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

azlB: pk - pk - mal

PKS_AT (581..879): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 233.5
Methylmalonyl-CoA: 231.5
Methoxymalonyl-CoA: 190.1
2-Methylbutyryl-CoA: 156.6
trans-1,2-CPDA: 138.1
Propionyl-CoA: 120.4
Malonyl-CoA: 116.9
CHC-CoA: 111.0
Isobutyryl-CoA: 83.3
Benzoyl-CoA: 60.3
3-Methylbutyryl-CoA: 47.0
inactive: 44.8
Acetyl-CoA: 44.2
fatty_acid: 30.1

PKS_AT (2313..2610): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 232.4
Methylmalonyl-CoA: 231.6
Methoxymalonyl-CoA: 191.0
2-Methylbutyryl-CoA: 158.1
trans-1,2-CPDA: 139.9
Malonyl-CoA: 120.6
Propionyl-CoA: 120.1
CHC-CoA: 112.9
Isobutyryl-CoA: 85.9
Benzoyl-CoA: 62.2
3-Methylbutyryl-CoA: 49.5
inactive: 43.9
Acetyl-CoA: 41.5
fatty_acid: 32.3

PKS_AT (3873..4177): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.7
inactive: 115.8
Methoxymalonyl-CoA: 73.6
Methylmalonyl-CoA: 64.8
Propionyl-CoA: 36.4
Isobutyryl-CoA: 31.2
Acetyl-CoA: 29.2
2-Methylbutyryl-CoA: 22.2
Ethylmalonyl-CoA: 19.7
Benzoyl-CoA: 14.5
fatty_acid: 12.6
trans-1,2-CPDA: 7.5
3-Methylbutyryl-CoA: 6.3
CHC-CoA: 4.4

azlC: pk - mal

PKS_AT (565..863): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 234.3
Methylmalonyl-CoA: 230.9
Methoxymalonyl-CoA: 187.9
2-Methylbutyryl-CoA: 153.2
trans-1,2-CPDA: 136.8
Malonyl-CoA: 115.9
Propionyl-CoA: 113.1
CHC-CoA: 110.2
Isobutyryl-CoA: 89.8
Benzoyl-CoA: 66.6
3-Methylbutyryl-CoA: 48.8
inactive: 45.6
Acetyl-CoA: 40.7
fatty_acid: 28.3

PKS_AT (2292..2593): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 238.8
inactive: 113.2
Methoxymalonyl-CoA: 77.8
Methylmalonyl-CoA: 65.2
Propionyl-CoA: 32.7
Isobutyryl-CoA: 28.9
Ethylmalonyl-CoA: 21.4
2-Methylbutyryl-CoA: 18.9
Acetyl-CoA: 18.3
Benzoyl-CoA: 13.6
fatty_acid: 10.0
CHC-CoA: 6.6
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

azlD: mal - mal - mal

PKS_AT (595..897): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 245.1
inactive: 116.9
Methoxymalonyl-CoA: 81.3
Methylmalonyl-CoA: 69.7
Propionyl-CoA: 40.0
Isobutyryl-CoA: 29.4
Ethylmalonyl-CoA: 28.4
Acetyl-CoA: 23.2
Benzoyl-CoA: 19.7
2-Methylbutyryl-CoA: 17.8
fatty_acid: 13.6
CHC-CoA: 12.2
trans-1,2-CPDA: 10.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2147..2448): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 243.5
inactive: 111.3
Methoxymalonyl-CoA: 79.7
Methylmalonyl-CoA: 68.1
Isobutyryl-CoA: 38.4
Propionyl-CoA: 32.0
Ethylmalonyl-CoA: 32.0
Acetyl-CoA: 23.0
2-Methylbutyryl-CoA: 21.9
Benzoyl-CoA: 19.5
fatty_acid: 13.0
CHC-CoA: 10.3
trans-1,2-CPDA: 8.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3694..3996): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.8
inactive: 117.5
Methoxymalonyl-CoA: 76.5
Methylmalonyl-CoA: 64.7
Isobutyryl-CoA: 36.6
Propionyl-CoA: 34.8
Benzoyl-CoA: 31.1
Ethylmalonyl-CoA: 25.0
Acetyl-CoA: 20.1
2-Methylbutyryl-CoA: 19.3
CHC-CoA: 13.9
fatty_acid: 13.8
trans-1,2-CPDA: 7.8
3-Methylbutyryl-CoA: 0.0

azlE: mal - mal - mal - pk - mal

PKS_AT (569..870): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 245.4
inactive: 122.9
Methoxymalonyl-CoA: 77.8
Methylmalonyl-CoA: 69.4
Propionyl-CoA: 39.7
Isobutyryl-CoA: 37.2
Ethylmalonyl-CoA: 30.5
Acetyl-CoA: 24.8
Benzoyl-CoA: 21.0
trans-1,2-CPDA: 19.0
CHC-CoA: 14.4
2-Methylbutyryl-CoA: 14.3
fatty_acid: 11.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (2121..2422): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 244.1
inactive: 118.0
Methoxymalonyl-CoA: 77.4
Methylmalonyl-CoA: 68.4
Propionyl-CoA: 38.2
Isobutyryl-CoA: 31.9
Ethylmalonyl-CoA: 26.4
Acetyl-CoA: 22.5
Benzoyl-CoA: 17.7
2-Methylbutyryl-CoA: 17.5
fatty_acid: 15.7
trans-1,2-CPDA: 14.3
CHC-CoA: 13.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (3673..3974): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 242.6
inactive: 115.8
Methoxymalonyl-CoA: 81.1
Methylmalonyl-CoA: 75.2
Propionyl-CoA: 39.4
Ethylmalonyl-CoA: 37.8
Isobutyryl-CoA: 33.0
Acetyl-CoA: 21.3
Benzoyl-CoA: 21.0
2-Methylbutyryl-CoA: 19.9
trans-1,2-CPDA: 16.0
CHC-CoA: 14.9
fatty_acid: 14.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (5043..5341): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 235.0
Methylmalonyl-CoA: 232.0
Methoxymalonyl-CoA: 192.0
2-Methylbutyryl-CoA: 157.0
trans-1,2-CPDA: 139.3
Malonyl-CoA: 120.3
Propionyl-CoA: 116.5
CHC-CoA: 113.3
Isobutyryl-CoA: 86.1
Benzoyl-CoA: 63.1
3-Methylbutyryl-CoA: 51.5
inactive: 51.2
Acetyl-CoA: 40.5
fatty_acid: 36.0

PKS_AT (6613..6915): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 238.9
inactive: 119.9
Methoxymalonyl-CoA: 77.6
Methylmalonyl-CoA: 67.6
Propionyl-CoA: 32.4
Isobutyryl-CoA: 30.2
Acetyl-CoA: 24.9
Ethylmalonyl-CoA: 18.9
Benzoyl-CoA: 18.8
fatty_acid: 16.9
trans-1,2-CPDA: 12.8
2-Methylbutyryl-CoA: 11.1
CHC-CoA: 7.0
3-Methylbutyryl-CoA: 0.0

azlF: pk - mal

PKS_AT (566..864): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 234.0
Methylmalonyl-CoA: 231.6
Methoxymalonyl-CoA: 188.6
2-Methylbutyryl-CoA: 153.5
trans-1,2-CPDA: 139.8
Malonyl-CoA: 119.3
Propionyl-CoA: 117.6
CHC-CoA: 111.6
Isobutyryl-CoA: 88.3
Benzoyl-CoA: 63.9
3-Methylbutyryl-CoA: 43.3
Acetyl-CoA: 38.3
fatty_acid: 37.8
inactive: 35.5

PKS_AT (2117..2417): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.0
inactive: 107.8
Methoxymalonyl-CoA: 67.3
Methylmalonyl-CoA: 58.5
Propionyl-CoA: 37.5
Isobutyryl-CoA: 30.1
2-Methylbutyryl-CoA: 16.1
Ethylmalonyl-CoA: 16.0
CHC-CoA: 9.0
Benzoyl-CoA: 8.7
Acetyl-CoA: 7.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

azlG: pk - mal

PKS_AT (574..872): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 236.3
Methylmalonyl-CoA: 232.2
Methoxymalonyl-CoA: 190.3
2-Methylbutyryl-CoA: 156.2
trans-1,2-CPDA: 138.5
Malonyl-CoA: 118.7
Propionyl-CoA: 115.0
CHC-CoA: 112.8
Isobutyryl-CoA: 82.1
Benzoyl-CoA: 62.0
3-Methylbutyryl-CoA: 46.3
inactive: 44.2
Acetyl-CoA: 40.0
fatty_acid: 35.7

PKS_AT (2144..2445): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 237.4
inactive: 119.9
Methoxymalonyl-CoA: 72.8
Methylmalonyl-CoA: 69.4
Propionyl-CoA: 39.5
Isobutyryl-CoA: 30.9
Acetyl-CoA: 23.6
Benzoyl-CoA: 19.8
Ethylmalonyl-CoA: 19.6
2-Methylbutyryl-CoA: 18.3
CHC-CoA: 13.7
fatty_acid: 9.3
trans-1,2-CPDA: 6.3
3-Methylbutyryl-CoA: 0.0

azlH: mmal

PKS_AT (578..876): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 227.9
Ethylmalonyl-CoA: 227.1
Methoxymalonyl-CoA: 185.6
2-Methylbutyryl-CoA: 160.5
trans-1,2-CPDA: 130.3
Propionyl-CoA: 114.8
Malonyl-CoA: 111.5
CHC-CoA: 105.3
Isobutyryl-CoA: 80.6
Benzoyl-CoA: 63.7
3-Methylbutyryl-CoA: 48.8
inactive: 42.5
Acetyl-CoA: 40.8
fatty_acid: 29.0

azlA: mal

PKS_AT (674..973): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 236.5
inactive: 139.0
Methoxymalonyl-CoA: 81.8
Methylmalonyl-CoA: 66.8
Isobutyryl-CoA: 41.8
Propionyl-CoA: 29.9
Ethylmalonyl-CoA: 28.4
Benzoyl-CoA: 27.6
Acetyl-CoA: 19.5
fatty_acid: 17.6
trans-1,2-CPDA: 16.7
2-Methylbutyryl-CoA: 11.9
CHC-CoA: 9.2
3-Methylbutyryl-CoA: 0.0

azl4: X

AMP-binding (9..375): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QYSDCGSNVK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

azl5: pk

PKS_AT (31..309): pk

ATSignature: (unknown)

No matches above 50%

minowa: CHC-CoA

Prediction, score:

CHC-CoA: 12.8
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor