BGC0001523 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001523 (Streptomyces sp. 211726)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	129,796	azalomycin F3a	PKS	100%

BGC0001523 - Region 1 - T1PKS

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Location: 1 - 129,796 nt. (total: 129,796 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 129,797
Label		BGC0001523

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001523	1.00	Polyketide	azalomycin F3a	Streptomyces sp. 211726
BGC0001700	0.46	Polyketide	niphimycins C-E	Streptomyces sp. IMB7-145
BGC0000041	0.36	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001163	0.34	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001647	0.33	Polyketide	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0001164	0.33	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000067	0.32	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000996	0.31	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0002729	0.31	Polyketide	sordarial	Neurospora crassa OR74A
BGC0002211	0.31	Polyketide	HEx-pks1 polyketide	Paraphaeosphaeria sporulosa

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001523	1.00	Polyketide	azalomycin F3a	Streptomyces sp. 211726
BGC0001700	0.76	Polyketide	niphimycins C-E	Streptomyces sp. IMB7-145
BGC0000041	0.68	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001163	0.67	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001647	0.66	Polyketide	lagunapyrone A, lagunapyrone B, lagunapyrone C	Streptomyces sp. MP131-18
BGC0001164	0.66	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000067	0.65	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000996	0.65	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0002729	0.65	Polyketide	sordarial	Neurospora crassa OR74A
BGC0002211	0.64	Polyketide	HEx-pks1 polyketide	Paraphaeosphaeria sporulosa

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 129796:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (pk - pk - ccmal) + (pk - ohmal) + (redmal) + (ohmal - ohmal - ohmal) + (ohmal - ohmal - mal - pk - redmal) + (pk - ohmal) + (pk - ccmal) + (Me-ccmal)
Non-colinear ordering:: azlB + azlC + azlA + azlD + azlE + azlF + azlG + azlH

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

azlB

PKS_AT (581..879): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 233.5
Methylmalonyl-CoA: 231.5
Methoxymalonyl-CoA: 190.1
2-Methylbutyryl-CoA: 156.6
trans-1,2-CPDA: 138.1
Propionyl-CoA: 120.4
Malonyl-CoA: 116.9
CHC-CoA: 111.0
Isobutyryl-CoA: 83.3
Benzoyl-CoA: 60.3
3-Methylbutyryl-CoA: 47.0
inactive: 44.8
Acetyl-CoA: 44.2
fatty_acid: 30.1

PKS_AT (2313..2610): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 232.4
Methylmalonyl-CoA: 231.6
Methoxymalonyl-CoA: 191.0
2-Methylbutyryl-CoA: 158.1
trans-1,2-CPDA: 139.9
Malonyl-CoA: 120.6
Propionyl-CoA: 120.1
CHC-CoA: 112.9
Isobutyryl-CoA: 85.9
Benzoyl-CoA: 62.2
3-Methylbutyryl-CoA: 49.5
inactive: 43.9
Acetyl-CoA: 41.5
fatty_acid: 32.3

PKS_AT (3873..4177): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.7
inactive: 115.8
Methoxymalonyl-CoA: 73.6
Methylmalonyl-CoA: 64.8
Propionyl-CoA: 36.4
Isobutyryl-CoA: 31.2
Acetyl-CoA: 29.2
2-Methylbutyryl-CoA: 22.2
Ethylmalonyl-CoA: 19.7
Benzoyl-CoA: 14.5
fatty_acid: 12.6
trans-1,2-CPDA: 7.5
3-Methylbutyryl-CoA: 6.3
CHC-CoA: 4.4

azlC

PKS_AT (565..863): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 234.3
Methylmalonyl-CoA: 230.9
Methoxymalonyl-CoA: 187.9
2-Methylbutyryl-CoA: 153.2
trans-1,2-CPDA: 136.8
Malonyl-CoA: 115.9
Propionyl-CoA: 113.1
CHC-CoA: 110.2
Isobutyryl-CoA: 89.8
Benzoyl-CoA: 66.6
3-Methylbutyryl-CoA: 48.8
inactive: 45.6
Acetyl-CoA: 40.7
fatty_acid: 28.3

PKS_AT (2292..2593): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 238.8
inactive: 113.2
Methoxymalonyl-CoA: 77.8
Methylmalonyl-CoA: 65.2
Propionyl-CoA: 32.7
Isobutyryl-CoA: 28.9
Ethylmalonyl-CoA: 21.4
2-Methylbutyryl-CoA: 18.9
Acetyl-CoA: 18.3
Benzoyl-CoA: 13.6
fatty_acid: 10.0
CHC-CoA: 6.6
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

azlD

PKS_AT (595..897): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 245.1
inactive: 116.9
Methoxymalonyl-CoA: 81.3
Methylmalonyl-CoA: 69.7
Propionyl-CoA: 40.0
Isobutyryl-CoA: 29.4
Ethylmalonyl-CoA: 28.4
Acetyl-CoA: 23.2
Benzoyl-CoA: 19.7
2-Methylbutyryl-CoA: 17.8
fatty_acid: 13.6
CHC-CoA: 12.2
trans-1,2-CPDA: 10.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2147..2448): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 243.5
inactive: 111.3
Methoxymalonyl-CoA: 79.7
Methylmalonyl-CoA: 68.1
Isobutyryl-CoA: 38.4
Propionyl-CoA: 32.0
Ethylmalonyl-CoA: 32.0
Acetyl-CoA: 23.0
2-Methylbutyryl-CoA: 21.9
Benzoyl-CoA: 19.5
fatty_acid: 13.0
CHC-CoA: 10.3
trans-1,2-CPDA: 8.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (3694..3996): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.8
inactive: 117.5
Methoxymalonyl-CoA: 76.5
Methylmalonyl-CoA: 64.7
Isobutyryl-CoA: 36.6
Propionyl-CoA: 34.8
Benzoyl-CoA: 31.1
Ethylmalonyl-CoA: 25.0
Acetyl-CoA: 20.1
2-Methylbutyryl-CoA: 19.3
CHC-CoA: 13.9
fatty_acid: 13.8
trans-1,2-CPDA: 7.8
3-Methylbutyryl-CoA: 0.0

azlE

PKS_AT (569..870): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 245.4
inactive: 122.9
Methoxymalonyl-CoA: 77.8
Methylmalonyl-CoA: 69.4
Propionyl-CoA: 39.7
Isobutyryl-CoA: 37.2
Ethylmalonyl-CoA: 30.5
Acetyl-CoA: 24.8
Benzoyl-CoA: 21.0
trans-1,2-CPDA: 19.0
CHC-CoA: 14.4
2-Methylbutyryl-CoA: 14.3
fatty_acid: 11.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (2121..2422): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 244.1
inactive: 118.0
Methoxymalonyl-CoA: 77.4
Methylmalonyl-CoA: 68.4
Propionyl-CoA: 38.2
Isobutyryl-CoA: 31.9
Ethylmalonyl-CoA: 26.4
Acetyl-CoA: 22.5
Benzoyl-CoA: 17.7
2-Methylbutyryl-CoA: 17.5
fatty_acid: 15.7
trans-1,2-CPDA: 14.3
CHC-CoA: 13.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (3673..3974): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 242.6
inactive: 115.8
Methoxymalonyl-CoA: 81.1
Methylmalonyl-CoA: 75.2
Propionyl-CoA: 39.4
Ethylmalonyl-CoA: 37.8
Isobutyryl-CoA: 33.0
Acetyl-CoA: 21.3
Benzoyl-CoA: 21.0
2-Methylbutyryl-CoA: 19.9
trans-1,2-CPDA: 16.0
CHC-CoA: 14.9
fatty_acid: 14.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (5043..5341): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 235.0
Methylmalonyl-CoA: 232.0
Methoxymalonyl-CoA: 192.0
2-Methylbutyryl-CoA: 157.0
trans-1,2-CPDA: 139.3
Malonyl-CoA: 120.3
Propionyl-CoA: 116.5
CHC-CoA: 113.3
Isobutyryl-CoA: 86.1
Benzoyl-CoA: 63.1
3-Methylbutyryl-CoA: 51.5
inactive: 51.2
Acetyl-CoA: 40.5
fatty_acid: 36.0

PKS_AT (6613..6915): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 238.9
inactive: 119.9
Methoxymalonyl-CoA: 77.6
Methylmalonyl-CoA: 67.6
Propionyl-CoA: 32.4
Isobutyryl-CoA: 30.2
Acetyl-CoA: 24.9
Ethylmalonyl-CoA: 18.9
Benzoyl-CoA: 18.8
fatty_acid: 16.9
trans-1,2-CPDA: 12.8
2-Methylbutyryl-CoA: 11.1
CHC-CoA: 7.0
3-Methylbutyryl-CoA: 0.0

azlF

PKS_AT (566..864): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 234.0
Methylmalonyl-CoA: 231.6
Methoxymalonyl-CoA: 188.6
2-Methylbutyryl-CoA: 153.5
trans-1,2-CPDA: 139.8
Malonyl-CoA: 119.3
Propionyl-CoA: 117.6
CHC-CoA: 111.6
Isobutyryl-CoA: 88.3
Benzoyl-CoA: 63.9
3-Methylbutyryl-CoA: 43.3
Acetyl-CoA: 38.3
fatty_acid: 37.8
inactive: 35.5

PKS_AT (2117..2417): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 217.0
inactive: 107.8
Methoxymalonyl-CoA: 67.3
Methylmalonyl-CoA: 58.5
Propionyl-CoA: 37.5
Isobutyryl-CoA: 30.1
2-Methylbutyryl-CoA: 16.1
Ethylmalonyl-CoA: 16.0
CHC-CoA: 9.0
Benzoyl-CoA: 8.7
Acetyl-CoA: 7.4
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

azlG

PKS_AT (574..872): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 236.3
Methylmalonyl-CoA: 232.2
Methoxymalonyl-CoA: 190.3
2-Methylbutyryl-CoA: 156.2
trans-1,2-CPDA: 138.5
Malonyl-CoA: 118.7
Propionyl-CoA: 115.0
CHC-CoA: 112.8
Isobutyryl-CoA: 82.1
Benzoyl-CoA: 62.0
3-Methylbutyryl-CoA: 46.3
inactive: 44.2
Acetyl-CoA: 40.0
fatty_acid: 35.7

PKS_AT (2144..2445): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 237.4
inactive: 119.9
Methoxymalonyl-CoA: 72.8
Methylmalonyl-CoA: 69.4
Propionyl-CoA: 39.5
Isobutyryl-CoA: 30.9
Acetyl-CoA: 23.6
Benzoyl-CoA: 19.8
Ethylmalonyl-CoA: 19.6
2-Methylbutyryl-CoA: 18.3
CHC-CoA: 13.7
fatty_acid: 9.3
trans-1,2-CPDA: 6.3
3-Methylbutyryl-CoA: 0.0

azlH

PKS_AT (578..876): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 227.9
Ethylmalonyl-CoA: 227.1
Methoxymalonyl-CoA: 185.6
2-Methylbutyryl-CoA: 160.5
trans-1,2-CPDA: 130.3
Propionyl-CoA: 114.8
Malonyl-CoA: 111.5
CHC-CoA: 105.3
Isobutyryl-CoA: 80.6
Benzoyl-CoA: 63.7
3-Methylbutyryl-CoA: 48.8
inactive: 42.5
Acetyl-CoA: 40.8
fatty_acid: 29.0

azlA

PKS_AT (674..973): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 236.5
inactive: 139.0
Methoxymalonyl-CoA: 81.8
Methylmalonyl-CoA: 66.8
Isobutyryl-CoA: 41.8
Propionyl-CoA: 29.9
Ethylmalonyl-CoA: 28.4
Benzoyl-CoA: 27.6
Acetyl-CoA: 19.5
fatty_acid: 17.6
trans-1,2-CPDA: 16.7
2-Methylbutyryl-CoA: 11.9
CHC-CoA: 9.2
3-Methylbutyryl-CoA: 0.0

azl4

AMP-binding (9..375): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QYSDCGSNVK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

azl5

PKS_AT (31..309): pk

ATSignature: (unknown)

No matches above 50%

minowa: CHC-CoA

Prediction, score:

CHC-CoA: 12.8
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0