LOCUS       BGC0001526             14417 bp    DNA     linear   BCT 05-SEP-2016
DEFINITION  Synechocystis salina LEGE 06099 bartoloside biosynthetic gene
            cluster, complete sequence.
ACCESSION   BGC0001526
VERSION     BGC0001526
KEYWORDS    .
SOURCE      Synechocystis salina LEGE 06099
  ORGANISM  Synechocystis salina LEGE 06099
            Bacteria; Cyanobacteriota; Cyanophyceae; Synechococcales;
            Merismopediaceae; Synechocystis.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:45:32
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..1674)
                     /gene="brtA"
     CDS             complement(1..1674)
                     /codon_start=1
                     /gene="brtA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045:glycosyl transferase group 1 (Score: 101.3;
                     E-value: 1.1e-30)"
                     /gene_kind="biosynthetic-additional"
                     /note="BrtA"
                     /product="glycosyltransferase"
                     /protein_id="AOH72619.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 1.7e-18,
                     bitscore: 56.3, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTSNIPLSPAIFYESEGYRTSGSKLMGRHSAGEAFLRAVIQGTP
                     PSNPIFGYCRDAGSFGAFKADVSRHDPTNGDRLVQWLPRHQPEKLGNASILYLPGPGLL
                     NEAQRRLGSGQEHSYSLCGITHTISSAGAMQSIRDLLVAPVHGWDAIICTSKAVKASLE
                     TIIAEQAEYLRVRLGARFCEMPQFPVIPLGVHCQDFQFTTAEKQAARARLGIAPEDVVV
                     LFVGRLVFHAKAHPLPLYLALERAAAEKSVTLIECGWFPNEPVARSYDQARQLAAPNIS
                     SIVLDGRQPSQRRDAWAAADIFTSLSDNIQESFGLTPIEAMAAGLPSVVSDWDGYKDTV
                     RDGIDGFRIPTTMPSDGGDDFALRHLLDIDSYDFYIGHVSQTVAVDIEAAALAFHQLLR
                     QPELRQRMGTAAKKQAQTVFDWSCIYGRYQELWHHLTEKRRQVSGDRQLPRGGAMIDPY
                     KLFANYPTNAMGNLQFALRENVTLAEIETIGQLESVKFASYVLPPMALVDQIVQNLNKS
                     QPLSLEELQSFVSVPMATLEKAISWLLKYGLIKSELILRN"
     source          1..14417
                     /country="Portugal: Moledo do Minho beach"
                     /db_xref="taxon:945781"
                     /isolation_source="intertidal zone"
                     /mol_type="genomic DNA"
                     /organism="Synechocystis salina LEGE 06099"
                     /strain="LEGE 06099"
     misc_feature    1..14417
                     /note="bartoloside biosynthetic gene cluster; brt"
     protocluster    1..14417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[6134:7286]"
                     /cutoff="20000"
                     /detection_rule="DarB"
                     /neighbourhood="20000"
                     /product="resorcinol"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6135..7286
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="DarB"
                     /neighbourhood="20000"
                     /product="resorcinol"
                     /protocluster_number="1"
     cand_cluster    1..14417
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="DarB"
                     /kind="single"
                     /product="resorcinol"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14417
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001526"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14417
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="resorcinol"
                     /region_number="1"
                     /rules="DarB"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            2378..4873
                     /gene="brtB"
     CDS             2378..4873
                     /codon_start=1
                     /gene="brtB"
                     /note="hypothetical protein; BrtB"
                     /product="haemolysin-type calcium-binding repeat"
                     /protein_id="AOH72618.1"
                     /transl_table=11
                     /translation="MANPFDVALTNLNSLYSGIPDVPVLQTYNSLNPYDISLTNLNNLS
                     NFVIEPAVLPTAAGTYGPQISEIAFALGGGIITGALADLLSTELGIEIENEALITKFLS
                     FWTPGSDYAFGQDYVEFIQGREGFDTIIGYDPGFNSALAPVQIDFVLGGPRQDFNFFAP
                     LDEPARYVLGDWRTPYYVDNDIQSEGLNEFMYVSLFTLNPDEFSPDNAENHRIQLYGSL
                     DNYYFVPTTVVIDGSALDPSLPSIPVPGTEIYYKTGQVFADLIAIVPFQDPNDPNLISA
                     WIDFEGYLPPEQADLSGQTANPLLSGSPLLQLGYEGIDMGTATALAPDGDIYVAGSTSS
                     LLGPFPRGGSDNFIARYNDDGSLEWIVQFGSTEFDIITDIVSDSAGNVYATGWTRGNVE
                     TGFGLAPGVQDNWVAKFDANGSQLWVEQFTIDGYLDRSMGIALDEVNGRIHLTGHSNTD
                     GSVADTNDPAIVPNGNVVTWIAGFDANTGAEDYRNVFEVAQTSNSRGRIDEGFGIDVDD
                     AGNVFSTGWAGENAYDVYLVKSDTDGAVLWTKSFGTGSNATQSYAWDVASDGTNAYILG
                     WTQGELNTFRDQIPPTDEFAGNATAPLTPQNAYQGGTFDAFVAAYDTDGTELWTWHVGG
                     TGDDGTFFGKIVAAGDFVYATGYTDGFIGTLGGSNAGDYDAWIGKFDKLTGQTAWIQQI
                     GSTKLDYATGISVDGDDIFVTGFTEGSLGGLNGGASDAWVAKLNQDGALEAFNVSAPIA
                     PPAPFAPPAPLTPPAPPAPDLSSLQSLGSTPQPTYPALGDSYGTNPFGTQPTQPTYDLS
                     SMYQNAYYGGFGGYSGYGY"
     gene            5103..6032
                     /gene="brtC"
     CDS             5103..6032
                     /codon_start=1
                     /gene="brtC"
                     /note="aromatase; BrtC"
                     /product="dialkylresorcinol-condensing enzyme"
                     /protein_id="AOH72617.1"
                     /transl_table=11
                     /translation="MTDSAAISILVLYYSQSGDTEQLVRNFVEPVASAGANITWANIQP
                     VEPYPYPWNLFRFFDVFPDCINGDTPEILPPSFEPDQHFDLIILAYQVWFLAPSLPIQA
                     FMKSPYAQVLRNTPVITLIACRNMWHSASEQLQQTLIDLGGHLIGKIVVTHQGSPLATF
                     ITTPRLVLTGKKDSALGVLPPGGFAEKDIKSVSQFGEQLVDYLPQLSLPQPPPLFIGMD
                     PVQVNKRYILPEWVGITLYRPWAKLISLGGPSGSPVRTLLVWLFALQLVIALPIVLLLC
                     TIIRILFHPILNPVLESYALKLQKQSFD"
     gene            6135..7286
                     /gene="brtD"
     CDS             6135..7286
                     /codon_start=1
                     /gene="brtD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     resorcinol: DarB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084:3-oxoacyl-(acyl carrier protein) synthase III
                     (Score: 183.9; E-value: 6.4e-56)"
                     /gene_kind="biosynthetic"
                     /note="ketosynthase; BrtD"
                     /product="dialkylresorcinol-condensing enzyme"
                     /protein_id="AOH72616.1"
                     /sec_met_domain="DarB (E-value: 1.5e-162, bitscore: 530.0,
                     seeds: 92, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKAYINGMGAFLPNEPINNAEIENVLGLINDKPSRAKKLVLRN
                     NGIKTRHYAIDPINRNITHTNVELTVESIKTLVKNSSFDLSQLDCLACGTSGPDQMIPN
                     HALMVHGALGNPPCEVIATSGVCCSGVAAFKYAYMNVSLGFARNAVVTGSEQVSSILRS
                     QHFQPEIDAKLAQLDDKPIVGFERDFLRWMLSDASGAALIENKPNDEGISLQIDWVDYI
                     SFANELETCMYGGCIKLEDGNVQGWKNINNLKEAFDEGYFDLNQDVKLLADNIVLMGQR
                     GLEKTKKKYGLDPNTIDWFLPHMSSGYFKKDLSLQMEEIGMFIPEERWFTNLEYKGNTG
                     AASIWIMLEELFNSGRLEKGQKILCIIPESARFSYAYMHFTVI"
     gene            7433..8629
                     /gene="brtE"
     CDS             7433..8629
                     /codon_start=1
                     /gene="brtE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MGT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1062:glycosyltransferase, MGT family (Score: 222.1;
                     E-value: 2.4e-67)"
                     /gene_kind="biosynthetic-additional"
                     /note="BrtE"
                     /product="glycosyltransferase"
                     /protein_id="AOH72615.1"
                     /sec_met_domain="MGT (E-value: 4.3e-40, bitscore: 127.5,
                     seeds: 193, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVALGKALSKKGHQVTLFCVDDFKEKFPQSAIQVQVIGKEEFPKG
                     TLPAFLEKLAQLEGVQAGMYTRDGLLAFAQVVLKEVPSLVKSHGVEALLVEQLCPEAGS
                     VAEYLQIPFVTTCAISPLNYELQVPPCFTDWPFQKEGWALARNRMGYFAYYQARRPIHQ
                     LIDKYRQDWDLPVLNHPDEAFSPIAQICQVPQSFEFPRHQLPPQFIFTGPFLDESSRPH
                     IPFPFEKLDGSPLIYASLGTTFNLDQTLFQRIATAVKDLNVQTVISLGGGLKSEELDNM
                     PNNVIVVDYAPQLELLQKASLTITHAGINTTLESLKNGVPLVALPVAGDQPSVAARIAW
                     TKSGEALPIARRSSQNLRAVIEKILDSPIYRQNAQRIQTDIEQAGGLQKAVEVIESCV"
     gene            8890..10170
                     /gene="brtG"
     CDS             8890..10170
                     /codon_start=1
                     /gene="brtG"
                     /gene_functions="transport (smcogs) SMCOG1029:RND family
                     efflux transporter MFP subunit (Score: 155.3; E-value:
                     3.4e-47)"
                     /gene_kind="transport"
                     /note="BrtG"
                     /product="DevB family protein"
                     /protein_id="AOH72614.1"
                     /transl_table=11
                     /translation="MGWFSFPPSRISDSQSQSGDNKQNQSSTAADLNGQQANVPSRVAA
                     IGYLEPKGEVRRLSGPLSPDGKGSRVERLLVKEGEFIRSGQVVALLDNYATMESKVRIA
                     QSQLQTAQAKLMQVQAGAKQGQISAQKARLSGVVSELEGQISIQNATIQRITAELAGEK
                     IAQEKSIEKLEIEYNNANSECQRYKLLFEQGGTSASEYDRFCLVPKQLERELAEAQANL
                     QRIISSRQEELAEARANLSRTVQTLNDQQNQALGTLDEISEVRGVDLAVAEAQVQEAMA
                     QLKQAQAEFNLTKIVSPTDGQVLKIYTYPGERISDKGIVEIGQTQTMYVRTEVYETDII
                     NVRKGQKAYISNPAFPEPLEGVVESVGHQIGRRTILDNDPVADIDARVVEVDIRLSEES
                     QNKIDLSRLTNLKTQVIIQTNSSKLQG"
     gene            10428..11501
                     /gene="brtH"
     CDS             10428..11501
                     /codon_start=1
                     /gene="brtH"
                     /note="BrtH"
                     /product="DevC family protein"
                     /protein_id="AOH72613.1"
                     /transl_table=11
                     /translation="MQLSFQDALFDSAVSLYEPLDADIFVLSSRSSALIAMENFPERRL
                     SQTLAVENVAEVIPLYLGFAQWRNPVQKNYWRSIYVIGVNPSQSVFNLKSVEENRFRLQ
                     LPDTVLFNEHSRAEYGPIPEIFQQKGELTTEIGDRGSSNREVTVVGLFPLGISFGADGT
                     ILTSDLNFRRILPGRKKGFINLGGIKLQDKSKLDITLEALRDYLPNDVKVFSKAEIFAF
                     EKNYWATGSPIGFVFALGVGIGLSVGVVITYQVLYSSISQHLSEYATLRAVGYRQRYLL
                     GVVFQEAVFLALFAYVPGIIFTSFATKLARDATNLPVYIDANKAIVVLLLSCLMCFVSG
                     GIAIRKLQDADPADVFY"
     gene            11555..12232
                     /gene="brtI"
     CDS             11555..12232
                     /codon_start=1
                     /gene="brtI"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 197; E-value:
                     6.1e-60)"
                     /gene_kind="transport"
                     /note="BrtI"
                     /product="DevA family protein"
                     /protein_id="AOH72612.1"
                     /transl_table=11
                     /translation="MNDAVFVSNLNHYYGHRQLRQQVLFDINLVINEGEIVLLTGESGS
                     GKTTLLSLIGALRSITEGNALVLNRQLYRANEARLVDVRRRIGYIFQSHNLLQFMTALQ
                     NVRMSAELLEEPHLGPATERAHRILDAVGLKSRKDYYPHQLSGGQKQRVAIARALVNSP
                     KLILADEPTASLDSKTGREIINLIQRLAQEQGSAVLLVTHDNRIFDIAHRILSMEDGRL
                     YET"
     gene            12314..13522
                     /gene="brtJ"
     CDS             12314..13522
                     /codon_start=1
                     /gene="brtJ"
                     /note="BrtJ"
                     /product="putative halogenase"
                     /protein_id="AOH72611.1"
                     /transl_table=11
                     /translation="MVSATSSSVENLDFSHLRGKDRKITQIIEKTYHNNTESDYTEKIE
                     QLEKAFNYSVEKDYYWGSPELSFLYGTPLYEQSSESQRKVLNHLYWIGQYNLVAASETS
                     TILFNQVTANVFFSLGGFDSLCHELDLETNQERYHIHAFQNIDRIVQEGIFGNADVNGD
                     QYRKQTLDVLRDGVARYMGSDLTLKFLSINWGSTPFLASQYYCARYIANISLKNKEQVY
                     SLHCRKLQKSGAHVPAPTAISRYHLLDESFHTTISQLLGKELYKEFAKPSAYEKFLMNT
                     VIYNYQKHGAGSLSGLAPGAPLKDELFMPVLYSVLRSSVFGMSTTEALDWIEKCACSEH
                     KGFHVSLEYVTRLLNDLRRFFSTIDYLWPVNKEWSIMAEAHSIPKTLKANRLAFRKFAR
                     TVY"
     gene            complement(14028..14417)
                     /gene="brtK"
     CDS             complement(14028..14417)
                     /codon_start=1
                     /gene="brtK"
                     /note="BrtK"
                     /product="endonuclease"
                     /protein_id="AOH72610.1"
                     /transl_table=11
                     /translation="MTQLKEGDFFLPYNKNLVERAREMRKNPTPAEGKLWNLLKGTRPF
                     GRDGIRVWRQRPIDHFIVDFYIPQLNLVIEVDGDSHFSPDGQAYDQERSRIIEGYDLTV
                     VRFTNHEVLHNLDEVWERICEFLPF"
ORIGIN
        1 ttaattcctt agtattaatt cggatttaat caagccatat ttcagtagcc aactgatggc
       61 cttttctagg gtagccattg gcacagacac aaatgactgt aattcttcca gagacaaagg
      121 ctgggattta ttgagatttt gcacaatttg gtcaaccaat gccataggtg gcaacacata
      181 gctagcgaat ttaacacttt ctaattggcc aattgtctct atttctgcca acgttacatt
      241 ttccctgagg gcaaattgta gattacccat ggcattggtt gggtagttgg cgaataactt
      301 ataagggtcg atcattgctc cccctcgggg taactggcga tcgccgctga cctgtcgacg
      361 cttttcggtc agatggtgcc acaattcctg gtaacgacca taaatacaag accaatcaaa
      421 aaccgtttgg gcctgttttt tcgctgctgt ccccatgcgt tgccgtagtt ccggttgacg
      481 taaaagctgg tgaaaagcta gagccgccgc ctcaatatcg accgcaacag tctgactgac
      541 atgaccaatg taaaaatcgt aggaatcaat atctaacaaa tgacgtagtg caaaatcatc
      601 acccccatca gagggcatgg ttgtgggaat cctaaagcca tcgatcccat cccgtaccgt
      661 atctttgtag ccatcccagt cactaactac agaggggaga cctgcggcca tcgcctcaat
      721 gggcgttaaa ccaaagcttt cttgaatatt gtccgatagc gatgtaaaga tatccgccgc
      781 cgcccaggca tctcgacgct gtgacggttg gcgaccatct agcacaattg agcttatatt
      841 aggtgcagcc aactgacggg cttgatcata actacgagcc accggctcat tcgggaacca
      901 accacattcg atcaaggtaa cggatttttc cgctgccgcc cgttctaagg ccagatacaa
      961 aggtaaggga tgggctttag catggaagac taatcgaccc acaaataata ccaccacatc
     1021 ctctggtgca attcccagac gggcacgggc agcctgcttt tccgccgttg taaactgaaa
     1081 atcttggcaa tgcaccccca ggggaatgac tggaaattga ggcatttcac aaaaacgtgc
     1141 ccccaaacga acccgtagat actcagcttg ttctgcgatg atagtttcta aactagcttt
     1201 cacagcttta gatgtacaaa taatcgcatc ccagccgtga accggagcaa ctaacaaatc
     1261 tcggatcgac tgcattgccc cagcggatga aattgtatgg gtgatgccac acaaactata
     1321 ggaatgctct tgaccactac ccaaacgtcg ctgggcttca ttgagcagac ctggccctgg
     1381 cagataaagt attgatgcat tccccaactt ttccggctga tgacgaggta gccactgcac
     1441 aaggcgatcg ccgttggtcg gatcatgtct tgaaacatca gccttaaatg cgccaaaact
     1501 tcctgcatct cgacaatagc caaatatggg attactgggg ggagttccct ggattacggc
     1561 ccgcaaaaac gcttcacccg cagagtgccg tcccataagc ttggaaccgc tagtacgata
     1621 accctctgac tcatagaaga tagccggaga caggggaata ttgcttgttg gcatcaatct
     1681 aaaaactcaa tttgcaacca tcttagtctg agttgttccc taatcgtttt tcggattaat
     1741 ccccaatgac atctagctag gacagtgggc aactccttta tcggtgaagg agatcgaggg
     1801 gattagttga aattgttttt aaccagcata ctgattagac tatgggcaat tttatccact
     1861 gtctggtaca aatcggtatc atttgtgttg ttatgcttcc tcaatgggtc tcaatgaatg
     1921 aagtcaagct taaattaaga ccttattaac tattttttgt cagactgtgt aggttgattg
     1981 gaggaaaacc acggaaagtt tctaagagca aaggtttcag gaagtgtact tgataaaaag
     2041 tcaatcccat atttcttgag ttatgaaaag cctaaaatgc tttcacagca agacctttaa
     2101 tgtcccaatg tcgagtccag cttacggggg ccatgaaagt aaggttagat taccaatact
     2161 gggatgagct gatgctaatc attgccgtca acacgaaaaa taaataagat taatgacaat
     2221 ttttggcatc ttgacttgca acccccgctg aattgactat aatcgcatag tcacactccg
     2281 taaattaaga attttttggt gaaactttct ctgattcttt aaagtttctt ctccgtaatc
     2341 gttaatcgct attcaatcta atcctggtga ggagtttatg gccaatccct tcgacgtcgc
     2401 tttaactaat cttaacagtc tttacagcgg tatccctgat gttcccgttc ttcagacgta
     2461 caactctctt aacccctacg acatttcttt aactaatctt aacaatctta gcaacttcgt
     2521 cattgagcca gccgtgctcc ctacagcggc aggtacctac ggccctcaaa tcagtgaaat
     2581 tgcttttgcg ttggggggtg ggatcataac aggggcacta gctgatctgc tatccactga
     2641 gttggggatc gagatcgaga acgaagctct gataactaag ttcctttcct tctggactcc
     2701 cggttccgat tacgccttcg gtcaggatta tgttgaattt attcaaggtc gggaaggctt
     2761 tgacaccatc atcggttacg accccggatt caacagtgca ttagctcccg tacaaattga
     2821 ctttgtcctt ggcggcccca ggcaggactt taacttcttt gcccccttag atgagccagc
     2881 acgctacgtt ttgggtgatt ggcggacccc ctattatgtg gataacgata tccagagtga
     2941 ggggctgaac gagtttatgt atgtttcgct cttcacactt aacccggatg aattttcgcc
     3001 agacaatgca gaaaatcatc gtatccagct ctacggcagt ctggacaatt actactttgt
     3061 accaacgaca gttgtaattg atggaagcgc cctagatcca agtcttccat caattcccgt
     3121 cccaggcaca gaaatttact ataaaaccgg gcaggttttt gcagatctga ttgccattgt
     3181 gcctttccag gatcctaacg acccaaatct tatttccgcc tggattgatt ttgagggcta
     3241 tctgcccccg gaacaggccg acctgagtgg ccagaccgcg aaccccttgc tctccggttc
     3301 tcccctcctt caattgggct acgaaggcat cgatatgggt acggcaacgg ccctcgcccc
     3361 ggatggtgat atctacgtcg ctggctccac tagcagtctt ttgggacctt ttccccgtgg
     3421 aggttcagac aacttcatcg cccgctacaa cgacgatggt agcttggaat ggattgttca
     3481 atttggtagc actgagttcg acataatcac cgacattgtc agcgacagcg ctggtaacgt
     3541 ttatgccacc ggttggaccc ggggcaatgt ggagacgggt tttggcctgg ccccaggtgt
     3601 tcaagacaat tgggtagcca aatttgatgc caatggcagc caactctggg tagagcaatt
     3661 taccattgat ggctacctcg accgttccat gggcattgcc ctcgatgaag tcaatgggag
     3721 aattcacctg acagggcact ccaacacgga tggctccgtt gcggatacaa acgaccccgc
     3781 cattgtcccc aatggtaatg tggtcacctg gattgctggt tttgatgcta acactggagc
     3841 agaagactat cgcaatgtct ttgaagtcgc ccaaacctct aacagccggg gacgcattga
     3901 tgaaggtttt ggcatagatg ttgacgatgc tggcaatgtg ttctccaccg gttgggccgg
     3961 agaaaacgcc tacgacgtct atctggttaa atccgatacg gatggcgctg tactctggac
     4021 caagagcttc ggaactgggt ctaatgccac ccaaagttat gcctgggatg ttgctagtga
     4081 tggcaccaat gcctatatcc taggttggac ccagggcgaa ctcaacacat tccgcgatca
     4141 aattcctccc acggatgaat ttgcgggtaa tgcgacggct ccacttactc cgcaaaatgc
     4201 ttaccaaggt ggtaccttcg atgcctttgt tgccgcctat gacaccgatg gtactgaact
     4261 ctggacttgg catgtaggtg gaacagggga tgatggaacc ttcttcggta aaattgtcgc
     4321 tgctggggac tttgtttacg ccacaggcta taccgatggc tttattggca ccctaggtgg
     4381 cagtaacgct ggtgactacg atgcctggat cggtaagttt gataagttaa ccggtcagac
     4441 tgcatggatc cagcagattg gtagcaccaa gctagactac gccactggta tttccgttga
     4501 tggcgatgac attttcgtaa caggctttac ggaaggttcc ttgggcggct tgaacggtgg
     4561 tgcttccgac gcctgggttg ctaagttaaa tcaagatggc gctttagaag cttttaatgt
     4621 ttctgcccct attgctcccc cagctccttt tgctccccca gcccctctta ctcctccagc
     4681 tcccccggct cctgatctca gtagcttgca gtcattgggt agcacccctc agccgaccta
     4741 cccggccctt ggggacagct atggcactaa cccctttggt actcagccta ctcaacctac
     4801 ctatgacctc agcagcatgt accaaaatgc ttactatggc ggcttcggag gttattccgg
     4861 gtacggctac taggatcggc ccattacgat ggtggactaa ataatctgga gggatagtaa
     4921 ttttatccct cctttgcttg tagttcctga tacttatccc tgaagctaag taggtgaggg
     4981 taaatatttg taaaatctcc ccgtcctacg gaatccctcc ttatcaaggg gggttggggg
     5041 gatagagtgc aaaacttatg cttggtttaa ttacaccgac ctaattaaaa tttgttttgg
     5101 taatgactga ctcagccgcc atcagtattc ttgtgcttta ttattcccag tccggggata
     5161 ccgagcaatt agtccgaaat tttgtagagc cggtagcatc agcgggagcc aacataacct
     5221 gggccaatat tcaacccgtt gagccctatc cctatccctg gaatctcttt cgcttctttg
     5281 atgtttttcc cgattgtatt aacggagaca cgccagagat tttacctcct tcctttgaac
     5341 ctgatcagca ttttgacctg attatcttgg cctatcaagt ttggtttttg gccccatccc
     5401 tccccattca ggctttcatg aagtcaccct atgcccaggt cctgcggaat acccccgtta
     5461 tcaccctcat tgcctgccgc aatatgtggc attctgcgtc agagcaattg caacaaaccc
     5521 tgattgacct cggcggccat ttaattggaa aaatagtggt gacccatcaa ggctctcctt
     5581 tggctacctt tatcactact ccccgcctag tgctcacggg gaaaaaagac tcggctttag
     5641 gggttctccc tcctggagga tttgcggaga aggatattaa atctgtgtca cagtttggcg
     5701 agcaattagt ggactatttg ccccaattgt ctctgccgca accaccaccg ctctttatcg
     5761 gtatggatcc cgtccaagtc aataaacgct acattttacc tgaatgggtt ggcattactc
     5821 tctaccggcc ttgggctaaa ctgatcagct taggtggccc gtccggatct cccgtcagga
     5881 ctttattggt ttggttattt gctttacaac tcgttattgc cttgccgata gtattgttgc
     5941 tatgtacaat catcaggata ttatttcatc ctatcttgaa cccggttcta gaatcctacg
     6001 ccctaaaact gcaaaaacag tcctttgatt agccttatag gctaagacta attcagtaga
     6061 taggcgacta aaattcaggg caatatatcg cccggataaa taataataac ctagttacag
     6121 aggagcaacc ctgaatgaat acaaaggctt atatcaatgg aatgggagct tttcttccca
     6181 acgaaccgat taataatgct gagattgaga atgtcttggg gttaatcaat gacaagccat
     6241 cacgggcaaa gaagttagtt ttaagaaaca atggaattaa aactcgtcac tacgctattg
     6301 atccgataaa tagaaatatt acccacacca atgtggaact aacggttgaa tcaattaaaa
     6361 ctcttgtcaa aaattcaagc tttgatctca gtcagttgga ctgcttggct tgtggaactt
     6421 ctggcccaga ccaaatgatt ccgaaccatg ccttgatggt tcacggcgcc ttgggaaatc
     6481 ccccctgcga agtcatcgcc acgagtggtg tttgttgctc gggagttgca gcctttaaat
     6541 atgcctacat gaatgttagt ctgggcttcg ctcgtaatgc cgtggtgact ggctcagagc
     6601 aagtatccag tatcctccgc tcccaacatt tccaaccgga aatagatgct aaattagccc
     6661 aactggacga taaacccatt gtcggattcg aaagggattt tttgcgctgg atgctttccg
     6721 atgcgtcagg ggccgctctc attgagaata agcctaatga tgaaggcatt tctctgcaga
     6781 ttgattgggt cgattatatt tcctttgcta atgaactaga aacctgtatg tacggaggct
     6841 gtatcaagct ggaagatggc aatgttcaag gctggaaaaa tatcaataac cttaaggaag
     6901 cttttgatga aggttatttt gatcttaacc aagacgttaa actgctcgca gacaacattg
     6961 ttcttatggg tcagcgaggt ctggaaaaga ccaagaaaaa gtatggttta gatcccaata
     7021 caattgattg gtttcttcct cacatgtctt ctggttattt caaaaaagat ttaagtctac
     7081 aaatggaaga aattggcatg ttcataccag aagagcgttg gtttaccaat ctggaataca
     7141 aaggcaatac tggagctgct tctatttgga ttatgttaga ggaattgttt aattctggac
     7201 gtctggagaa aggccaaaaa attctctgca ttattccaga aagtgcccgc ttttcctatg
     7261 cttatatgca ctttactgtc atttaggtca ttgagcagag ccagggggtc tgatcactag
     7321 ataaatacca tattcatagt catcaaaact gaattattta cggttacttt gagagaactc
     7381 catgactcat ttcggactga tttgtcctca atcgacaggc caccttaatc cgatggttgc
     7441 actgggcaag gccttgtcca aaaaaggaca tcaggtaact cttttttgtg ttgacgactt
     7501 taaagagaaa tttcctcagt cagccatcca ggtacaagta atcggaaaag aagaatttcc
     7561 caagggtaca ttacctgcct ttttggaaaa attagctcaa cttgagggcg ttcaggcagg
     7621 catgtacacg cgagatggtt tactggcctt cgcccaagtt gtattgaagg aagttccctc
     7681 ccttgtcaag tctcatggtg ttgaagcgtt gctagtggag caactttgcc cggaagctgg
     7741 aagcgttgct gagtatctac aaattccctt tgtcaccact tgcgctattt ctccacttaa
     7801 ctatgagtta caagtccctc cttgcttcac agattggcct tttcagaaag aaggttgggc
     7861 attagcgcgt aatcgtatgg gatattttgc gtattatcag gcccgtcgtc caattcacca
     7921 acttatagat aaatatcggc aggactggga cttaccagtc ttaaatcatc ctgatgaggc
     7981 tttttctccc attgctcaaa tttgccaagt tccccagtca tttgagtttc cccgccatca
     8041 attacctcca caatttattt ttacgggacc ttttttagat gaatcaagcc gtccccatat
     8101 tccttttccc tttgaaaaat tagatggcag tccgcttatt tacgcctcgc tgggaaccac
     8161 atttaattta gatcaaaccc tctttcaacg aattgcaacg gcagtcaaag acctcaatgt
     8221 acagacagtg attagtctcg gtggtgggtt aaagtctgaa gaactagata atatgcctaa
     8281 caatgtcatt gtggtagatt atgcacctca attggaacta cttcaaaaag cctcgctgac
     8341 tattacccat gctggtatta atacaacttt ggagtccttg aaaaatggtg ttcccttagt
     8401 tgccttgccg gtggcgggag atcaaccgag tgtggcagct cgcattgcct ggacaaaatc
     8461 tggtgaagcc ctgccgatcg cccgccgttc ttctcagaat ctaagggccg ttattgagaa
     8521 gattttggat agtccaattt atcgccaaaa cgctcaacgt atccagactg acattgagca
     8581 agcaggaggt ttacagaaag cagtggaagt tattgagagt tgcgtctaaa ttatcttccc
     8641 tggccaactc caaaaagtat gaagataatt cggaacggaa aaacgaaata aggcaactga
     8701 caattatccc ttgtactata cacgtcggga tgattgtgct accatggact agccactttt
     8761 acagaatggc gatcgccgta gcattgcaga ggatttatcg caatgaagtc agttttcccg
     8821 aataattcct caagcagcag gctcgttctc ttcctcgttg ttggtgttgt attcgcaggg
     8881 gtcgggattt tggggtggtt tagttttccc cccagtagaa tatctgattc tcagtcccag
     8941 tctggtgata ataaacagaa tcaaagctcg acagcggcag atctcaatgg tcagcaagcc
     9001 aatgtgccat cccgagtggc agccattggt tatctggaac ctaaggggga agttcggaga
     9061 ctttctggtc cccttagtcc cgatggcaag ggcagtcggg ttgagcgact tttggtcaaa
     9121 gagggggaat ttatcagatc tggacaggtg gtggcccttc tggataacta cgccacaatg
     9181 gaatcaaagg taaggattgc ccagtcccaa ctgcaaacag cccaagccaa gttaatgcag
     9241 gtacaggcgg gggccaagca ggggcaaatt tcggcgcaaa aagctcgatt atctggtgtt
     9301 gtgtccgagc tagaggggca aattagcatc cagaatgcaa ctattcagcg gattactgca
     9361 gaattagcag gggagaaaat tgctcaagaa aaatccatag aaaaactgga aattgagtac
     9421 aacaatgcca attcagaatg ccagcgctac aaattgctct ttgaacaagg aggtacttct
     9481 gcctccgaat atgatcggtt ctgtttggtg ccgaaacaac tagagcggga actagcagag
     9541 gcccaagcta atttgcaacg aattattagc agccgccaag aagaacttgc ggaggccagg
     9601 gcaaatctga gtcggactgt gcaaacgctc aatgatcaac aaaatcaggc cttgggaact
     9661 ctggatgaaa tttctgaagt taggggagtt gatctggccg tcgcagaagc ccaggtacag
     9721 gaagcaatgg cgcaactcaa acaagctcag gccgaattta atttaactaa aattgtttcg
     9781 cccactgatg ggcaagtcct caagatttat acctatccag gcgagaggat tagtgataaa
     9841 ggcattgttg agattggaca aactcaaaca atgtacgtaa ggacggaagt ctatgaaacc
     9901 gatattatca atgtcagaaa gggtcagaaa gcctacattt ctaatccggc ttttcctgag
     9961 ccactggaag gggtggttga gtccgttggt catcaaatcg gtcgtcgcac aattcttgat
    10021 aacgatcccg ttgcggatat cgatgccagg gtagtcgaag tggatatccg cctgtcggag
    10081 gagagccaaa ataaaattga tctttcacgg ctaaccaatc ttaagactca ggtgattatt
    10141 caaactaatt cttcaaagct acaaggctaa gcggcaaaaa tcggtggcct ggtaagatgt
    10201 ccttcgacta gctctagcta tttcgctgat ttgattggag cggtcaacac catgtttttt
    10261 tagattaaac tggtgactgc ctagcttgga agtaaacatt tgacattaaa aaaaattgtt
    10321 agtttttttc tgtgaagata cctactgctt ggcttcagct caaattctat ccactgcgtc
    10381 tcgcggtggc ggtggcgggg gtctgttttt caatattttt aatttttatg caattgagct
    10441 ttcaggatgc tctcttcgat agtgccgtat ctttgtacga gcctctggat gcggatattt
    10501 tcgttctgag tagtcgctcc tcagctttaa ttgccatgga aaactttccg gaacgaaggc
    10561 taagtcagac cctggccgtg gagaatgttg cggaagtaat cccgctctat ctaggctttg
    10621 cccagtggcg caatccagtt caaaaaaact attggcggag tatttatgtt attggcgtca
    10681 accccagcca gtctgtattt aatttgaaat cagttgaaga aaatcgcttt agactgcaac
    10741 taccagatac ggttcttttt aatgagcatt ctcgggcaga gtatggcccc attccagaga
    10801 ttttccagca aaagggagaa ttgacaaccg aaattggcga tcgtggttct agtaatcgcg
    10861 aagtgaccgt tgttggcttg tttcctttag gaatatcctt tggtgccgat ggcactattc
    10921 ttaccagtga tctaaacttc cggcggatat taccgggacg caaaaaagga tttattaatt
    10981 tggggggcat taaactccaa gacaaaagta agttggatat caccctggaa gcactcaggg
    11041 attatttacc gaacgatgtc aaggtttttt caaaggcaga aatttttgcc tttgagaaaa
    11101 actactgggc cacgggcagt cccattggtt ttgtctttgc tttaggagtc ggaatcggtt
    11161 tgtctgtcgg tgtggtgatt acctatcaag tgctctacag tagcatctct cagcatctgt
    11221 cggaatatgc aactttgcgg gccgttggtt atcgacagcg ctacctactc ggagttgttt
    11281 ttcaagaggc tgttttttta gccctatttg cctatgttcc gggtattatc tttacgagtt
    11341 ttgccaccaa attggcgagg gatgccacca atcttcctgt ctatatcgat gcaaacaaag
    11401 caatagttgt attgttgcta tcatgtctga tgtgttttgt atcgggtgga attgccattc
    11461 gtaaacttca ggatgctgat ccggcggatg tgttttatta gaattgaagc agttaatata
    11521 tttttaactc caggagcaat ttcacttact ttgcatgaat gacgctgttt ttgttagtaa
    11581 tctcaaccac tattacggcc atagacaact ccggcagcag gttttatttg atattaatct
    11641 ggttatcaat gaaggggaaa ttgttctact aacgggggaa tcgggttcgg gcaagacgac
    11701 cctactgtct ttgatcggcg ctctgaggag cattacggag ggaaatgccc tagtgctcaa
    11761 tcgtcaattg tatcgagcta atgaggcccg attagtggat gttcgccgga gaattggtta
    11821 tattttccaa tcccataatc ttctccaatt tatgacggct ctgcaaaacg tcagaatgtc
    11881 ggcggagcta ttggaagaac ctcaccttgg cccagcaaca gagcgagccc atcgcatcct
    11941 ggatgctgtc ggtttaaaat cacggaaaga ttattatccc caccaactat cggggggaca
    12001 aaaacaacgg gtggcgatcg cccgtgcctt ggtcaatagt cccaagctaa ttttggcgga
    12061 tgagcccact gcatccttgg atagcaaaac cggtcgggag attattaatc tcatccagcg
    12121 actggcccag gaacaaggaa gtgcggttct cctggtcacc cacgataacc gtatttttga
    12181 cattgcccac cgcattctca gcatggaaga tggccgactg tacgaaactt agagcagtgt
    12241 tatagtgggg gtagttataa ggcttggcac aaaaaaagtc caccctgtat tatttctatt
    12301 tatttgtaaa tccatggttt ctgcaacgtc atcctctgtt gaaaacttag attttagtca
    12361 tcttcggggt aaagatcgta agattaccca gatcatcgaa aaaacctacc acaacaacac
    12421 ggaatctgac tacaccgaaa aaattgaaca gttggaaaag gctttcaact actcggttga
    12481 aaaagattat tattggggct ctccagaatt atcatttttg tacggaacac ctctttacga
    12541 gcagtcttca gagtcgcaaa gaaaggttct gaatcatctc tactggattg ggcagtataa
    12601 tctcgttgcg gccagcgaaa caagcaccat tctttttaat caagtcacgg caaacgtttt
    12661 cttctccctg ggaggatttg actccctctg ccacgagctg gatctggaaa ccaatcaaga
    12721 acggtaccat attcacgctt ttcagaatat tgatcgcatt gtccaagagg gcatctttgg
    12781 gaatgctgac gtcaatggag atcaataccg caagcagacc ttggatgttc tgcgggatgg
    12841 tgttgctcgc tatatgggat cggatctaac cctaaaattt ctcagcatca attggggcag
    12901 tactcccttt ctggcgtctc aatactattg tgctcgatat attgccaata tttccctcaa
    12961 aaataaagaa caggtttatt ctttacactg ccgtaagcta caaaagtccg gtgctcatgt
    13021 tccggcaccg acggccattt ctcgttacca tcttttggat gagtcattcc atactactat
    13081 ttctcagtta cttggcaagg aactttataa agagtttgcg aagccatctg cctacgaaaa
    13141 gtttttgatg aatacggtca tctataacta tcaaaaacac ggggctggta gtttatctgg
    13201 cttggcccct ggggcacccc tcaaagatga gctgtttatg cccgttctgt atagcgtcct
    13261 tcgatcttca gtgtttggga tgtccaccac ggaggctttg gattggattg aaaaatgcgc
    13321 ctgtagcgaa cacaaaggat ttcatgtctc cttggaatat gtcacccggt tattgaatga
    13381 cttgcggcgt ttcttttcga ctattgatta cttatggccg gtaaataagg agtggagcat
    13441 aatggcggaa gcccattcta ttccaaaaac gttaaaggct aatcgccttg catttaggaa
    13501 gtttgccaga acagtctatt gacatccgcc gccgccctaa agggtacgga ttccttacca
    13561 cgcctcaacc taaaaaagga atcgttcact gaatgggttt gacttttggc tggagaaatc
    13621 ctgtcagggt aagttgagtc tgtgaacaaa gaatcctcgc cgttcacggc ggggagtgtc
    13681 aaactccacc gggtcgttcc agcagagcag tcttgcaatc cctctgcgtc cgctttaagt
    13741 ctccgaatac cctccggcga ctaagtttgg cgcggtctac tgaattttgc tttttgcctt
    13801 caaggttgaa ctttagagca ttgcctctcc agcgttgtct gcgcctactc gcaacgtagc
    13861 tatttcaaca tgtcttgagt ccagagggag aatatgaaca ccgttttgat gttcttgggc
    13921 tagtttcgtt tttggatgag gacagaaata ataaatccaa ttcccccctt tcttaagggg
    13981 ggttaggggg gattaaaaca ccaaaattta aaaggattta gaggagatta aaaaggcaaa
    14041 aactcacaaa tacgctccca aacttcatca agattatgga gaacttcatg gttggtgaaa
    14101 cgaacaacgg ttaagtcata tccctcgata atacgggatc gctcttggtc gtaggcttga
    14161 ccatcagggg aaaaatggga atcaccatcc acttcaatta ctaggttcaa ctggggaata
    14221 taaaaatcga caatgaaatg atcgatggga cgttgcctcc agactcgaat gccatcccga
    14281 ccaaatggcc tagtaccctt taataagttc cagagttttc cctctgccgg agtgggattt
    14341 ttccgcattt ccctggcccg ttcgactaaa ttcttattat aggggaggaa aaaatcgcct
    14401 tctttaagtt gagtcat
//
