LOCUS       BGC0001528             21000 bp    DNA     linear   PLN 18-MAR-2021
DEFINITION  Neocamarosporium betae PS-13 DNA, betaestacin biosynthetic gene
            cluster, complete sequence.
ACCESSION   BGC0001528
VERSION     BGC0001528
KEYWORDS    .
SOURCE      Neocamarosporium betae
  ORGANISM  Neocamarosporium betae
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae;
            Pleosporaceae; Neocamarosporium.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 02:21:08
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 21000
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..21000
                     /db_xref="taxon:1979465"
                     /isolate="PS-13"
                     /mol_type="genomic DNA"
                     /organism="Neocamarosporium betae"
     subregion       1..21000
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Terpene"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..21000
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(2982..4165)
                     /gene="btcD"
     CDS             complement(join(2982..3124,3176..3567,3605..3926,
                     4003..4165))
                     /codon_start=1
                     /gene="btcD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1121:dioxygenase, TauD/TfdA (Score: 162.8; E-value:
                     1.7e-49)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative alpha-ketoglutarate-dependent
                     dioxygenase"
                     /protein_id="BBE36499.1"
                     /transl_table=1
                     /translation="MALIKEPLKPTGALDEFRSFDVTPIIGTEFPDASLKAWLEDPKAD
                     DLLRELAITVSRRGVVFFRRQDGLTEEMQKAIVQKLGVLSGKPATSSLHRHAHQPDPNA
                     DPEILWINSEENKKLLAGTPFDPALPARQSCRGLWHNDISYEPNPSDYALLRITQPPAL
                     VVADLPAIDTLWASGYEVFDRISRPIQKFLETLTATFGELRERDGSDPKFQVPRGSPAN
                     VGTNLRPTHPVIRTNPVTGWKSVYAVGLHVQTINGLASDESDGLKKWFTKLIVENHDLQ
                     VRFRWNNANDVAIWDNRCTYHTATYDHEGYGIREGYRACGVGEKPYLDPNSTGRREAR"
     misc_feature    2982..16881
                     /note="betaestacin biosynthetic gene cluster"
     gene            complement(9171..10933)
                     /gene="btcC"
     CDS             complement(join(9171..9539,9603..10510,10574..10683,
                     10740..10852,10901..10933))
                     /codon_start=1
                     /gene="btcC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 293.8; E-value: 4.1e-89)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative cytochrome P450"
                     /protein_id="BBE36500.1"
                     /sec_met_domain="p450 (E-value: 5.1e-69, bitscore: 221.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MSFPGVIFVVSFFPLLMGIAVYRLLWHPLASFKGPKLAAVTDWWF
                     CKKWLNGRYHQTLEKLQLQYGDVIRIAPNELVFATPEAARDIYTRCNPDQDLQFIKQPP
                     FYKQSEGFPTLVTETDPAAHRTLRKPLERGFSPSSLKDYGRVIERVADDLTAQLEKASE
                     HSNAVDVKAWAARFTFDVITEVTFGKSSGTVAQGKNTVWLDLLTGNIAAAAVGVAIRRQ
                     PHAVKTLLRSIFAKLSKTAKLRAQYLSVCRKMCEERLRDPPKAANLFDHVLATCPPVEK
                     DADNHGYLVFLQGQAAALVSGGTETSSTLLSSLIYNLLAHPAHLARLQYEVRNAFSQSG
                     EIDIESTKQLKYLQAVIDESLRIFPPVGFGLPRVCPGAMIGGVYVPKGTVVQAPDILMV
                     RNSRYFVRPYEFLPERWLPKDHEFYDEQFSGDRKEASKPFSLGPRQCIGMSLAYAEWRI
                     VLAKLVLKFDWEIIGETQDLMDVARLKLLWEMPPILVSFKPVGGLAAASPGA"
     gene            complement(11716..13533)
                     /gene="btcB"
     CDS             complement(join(11716..12436,12494..12665,12724..13128,
                     13192..13342,13396..13533))
                     /codon_start=1
                     /gene="btcB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 248.3; E-value: 2.5e-75)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative cytochrome P450"
                     /protein_id="BBE36501.1"
                     /sec_met_domain="p450 (E-value: 1.1e-58, bitscore: 187.1,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MDAMNGSDVQSDGLPNGNTLQLAYHVMPRLVQDMGPSLLLIFGPL
                     VLVSSCIYYLFFHPLSKIPGPKLAAISPVWMMKSLYGKKLNRDIKALHDRYGDVVRVGP
                     NTVSFATVGALSSVHHEKAGGDDGTFTKEGTIEWLLGLMTWPAQNILTKTDVKGHARLR
                     KAVQPAFSAKELRKQEPIEQEWITKFNRLLDAAAVDNTEVNITEHMSHLVWDMLSDLSF
                     GEPLARSQLVKFNRLKWLACAMSPMLEVMQSILTFPIAGAFTRWIVTSFPQVFQLPRDI
                     LPTGTLRTTLERADRGHDFLSAIVGAQEKGIVLSQDELQSNSAMLVMVGQDATVTCLSS
                     TLYFLLRDPQHMANLQDEVRGQFKSEDEINGLAIARLPLLNGSINEAMRLLSPANGTGT
                     HRLSNGGFVEGIYLPAGITVAVDQYTIQRSPKYWRDPETYRPERWASVGEPESEFENDV
                     HSAYRPFLLGPRACLGRELALQIVRLVLARLVFSYDLRMVNRDFVWERDCDSSYLWLGH
                     KVVVKVQKRES"
     gene            order(14494..14794,14861..15155,15300..16881)
                     /gene="btcA"
     CDS             join(14494..14794,14861..15155,15300..16881)
                     /codon_start=1
                     /gene="btcA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182:Polyprenyl synthetase (Score: 235.1; E-value:
                     1.5e-71)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative bifunctional terpene synthase"
                     /protein_id="BBE36502.1"
                     /transl_table=1
                     /translation="MANPPVTEWKQSEAIPAHVAQETGCFTTLPIRIHKNNSIADQATL
                     ESINDWKQHLDDGWESRSGSAISKVGNWCSFIFSEALPERLPCITYLANIGNIHDDATE
                     DATLDEAITSHAQFSTALSLDTDDEPSLQEAKTRRFRHLVSNCVLEILSIDRDMGTRMI
                     VSYQKKWLDVMEHLNYEGIESLEEYLEFRMLNGGMEPFWLMCQFGMGLNIPDAELAPTR
                     HIFEPAEWALVLTNDYWSWGREYNASLTKGSRIVNSIELLSRLRNISYDEAKEVVRDLI
                     TTYEAEYERRVQDFLRENPSTQMYLRQFIEVAGLVVAGNHYWCANCPRHHAWKEQDQAV
                     HAVDQLEEFPAVEAQSPSSATHTAAPQEDPPVTEAPSPISSPSSSSSAKPSSSSAADSS
                     SCTSTSQHSPSETDSTPPSSLHLLKSPHPVDAPCTYMSSLPSKGVRSTLIHALNQWFQI
                     SSRNVTLIKEITSMLHNSSLILDDIQDQSPLRRGKPAAHTIFSTAQSINSSTYLFVRAM
                     QMVSENFESSVQMSFLEILQRMHIGQSYDLHWRFHLQCPTEDEYFEMVDAKTGCMFEML
                     LLLMSSKSRHVKDHSFTSFIRTFGRYFQVRDDYMNLTSGEYTAGKGWCEDLDEGKLSYP
                     LIECQKMAPASFTQIMGIFRAKQADTAQLSDETKKYVIELFRKSGVLDSTLDWIMEMER
                     QLLMEVKRLEGVMGDSNPMLYVLLQTLSLNQ"
ORIGIN
        1 cttgagggtg gtgaaatcgg gggtgatgcc ggaggaggtc aagtacacaa cgcctgtgaa
       61 gggctcgttg aggagggagc cgatggactg cacggggaag ccacgtgcgg cagcggcgag
      121 ggtgtggatc atggctttga agccgagatc gactttcccg gagccaatga tctcggtgac
      181 gtcctgagaa ggttagtcac gaatcttttc atgagagttc gaattcgtac gctgggatca
      241 ttcggttcaa gaagagcaac tttgatgcct tcatccttaa agtatccctt gctctgagca
      301 aggtacagag gggcgtggta aggcgtcgcg tgcctatatc ttgttaacct aatacacctt
      361 ccatccaaga tgcatagcgc accagttggt caagaaggta atcttatcag tcgacatctt
      421 gacttattca cagaagctaa tcaagtcagc acgccaacat tgaggggaga agggcatcat
      481 gaagcagcag tagtccttcg cgctggaatt atccagatca agtttaaacg gtataatctc
      541 agaacaagcc gccagcgacc caaaggtcat tgacagcttg aacacccgcc atgctttgct
      601 tcagatggtt gagatgggta cctgatgatg ccatttcgga catctgttac ttgaccgaga
      661 catcctggtg agcgggaaga taggccgata tggcccttca acgctgcttt gatggtagca
      721 tcgtaccgat gtagtctggc ggtggtaatt ttgatggtaa caatgtatct gggtctgcca
      781 aaagaatacg atacgtcgtc gcgatggaaa tacgcatact ttcaaatact tatagacacg
      841 attctagttt gttgggctcc cccacgacgg gtaaattgct catgtgcgga tacaggtgta
      901 ttggtaaatg gtggggcagt gaggggtaca gcttccaacc acgtcaagac gcatcactca
      961 tccacgagtc cacgatacaa ttccaccaac cgcataacat gcagagatcc ggcccaagct
     1021 atttcgcgca aatttgctgg tgctaaagtg ttgagccgat gatcataact cgggataccc
     1081 ttttgggttt gctctttacc gcattctgca ctcgcgaaag cagcatgcat acgttgagtc
     1141 ttcctgataa cccgcgtttt cggttccctg gagtcatcat tccagctgat ggcgatgccg
     1201 tggtctgacg agtttccaat tggctccatt tcgagatcag caggagataa ggaagcgctg
     1261 tgtcttcgca gacattcagg cgctcaggat atacggattg gaaacagaag ggatggcagt
     1321 gtgaaatctg attagtacct taagcgtggc tagcagttga ttagctgcag ccatgattat
     1381 gttaagcacg gacgtcacca gtattggaga tacgcaggta gcacgctgca cgcgtacact
     1441 gggtactact acgtgcacgg tcgccaacac caaagacgcg atgcgaggca tggaaatatt
     1501 gtgttacgct ctggtctaag catactactc gaccccaatt acctttctat tcaagccatc
     1561 gccctggagc tcccttgaga atagcctgtc ccaatccggc ttcagagccc cgcggttatc
     1621 aaagaagttc ggaaaatggg cgcgatacct agcccataaa cctggcacgg ccgcttcctc
     1681 tggcaatcct ttgactactt gtccaagggt gtgaaagtcg ccacacacac catcttcaaa
     1741 gccaaacaaa ggaataagcg gcccctgttg aaagccccag aaggatccac gcgctttagc
     1801 tgacataacg gattcatctc gaatctccgc aatgtctgcc acgaccataa ttgttagctg
     1861 cgtatatgtt ctcgcaccgt gctttccatc tgtccactgc ggctgtacct ggatcaaggc
     1921 gactctgctg tgatcttctg gcatcagacc agcaataacc tttccttcgt acgatggaat
     1981 tcctggccgc tcaatcacaa gtcgcaactg cgtcttgctc tccctatgta tcgtggatgt
     2041 gccgggaggg tgactaaatt atcaaaagtg ttagagaata tcgacgtcga caaagctcga
     2101 catgggccat catggtactt acaagaaggg tgctggtatc ttcatcgcac ggccacttaa
     2161 agcgttgtag acaacactct cttcatctgc accgggcatg atgctgtcga acaggactag
     2221 ctgcacccag tcgatttcca ttctttcagg tccatgataa cgtattcggt aggaaataag
     2281 attgtgagcc ccaagcatga gcaactgtgg ctggttcgtg gtttgaacac tgacaactcc
     2341 gctggtccac aaattcaggt gtggtgtatt agtatcggga ccggagaata tcttttgata
     2401 tatggaaaat atgtcaatgt cgtccggcag gggatgcagt tgaggaacac catttagcgg
     2461 atcatctggc tcggatcctg ggaaaggcat tgcaccgcgt tgtatatttt gtaatatcca
     2521 accactgcgg ccatctatgt ggtcaaactc tcacacggaa atacgacaac aagtagtatg
     2581 aggaagctgg taacttctaa ggattcgtgt caactggttg accgaagcac taccaactca
     2641 caagcttcgc ttatgaacaa catcaaaaca atcaactaca gcactcagat gactgatctt
     2701 gagaatccac ctcctccttt gatcacaaac tgcctgctcc tggaatcggg ccgcaatggc
     2761 actgatacaa acacgaagcc accttgcgga aggcggggat ggcaatcact ctcccagccc
     2821 caccaagaat gagatgctat actgcatacg tattggagta tggttgccat tgtatatggt
     2881 gatttttgag ctagcggggt gtctacgtac acgcctgatt catggctcac tgcggcccga
     2941 tcctagttca ataaggtttc ggcacggcct ttctcctaac cttaccgcgc ttccctccga
     3001 ccagtactat taggatctag atacggcttt tctccaactc cacacgcacg atagccttcc
     3061 cgaatcccgt atccctcatg gtcgtacgtg gcggtatgat acgtacagcg gttatcccag
     3121 atagctttat ttgtcagtaa gagatgcatt aacatgggac atgaagacaa cataccgacg
     3181 tcattggcgt tgttccaacg gaagcgcact tgaagatcgt ggttctcgac aatcagtttg
     3241 gtaaaccatt ttttgagccc gtcactctca tcactggcaa gtccattgat cgtttgcaca
     3301 tgaagaccga cagcatagac acttttccaa cccgtgacag ggttcgtgcg gattaccgga
     3361 tgcgtcggtc ggaggttcgt gcccacattc gctggggacc cacgtgggac ttggaattta
     3421 ggatctgaac cgtctctctc gcgcagctcc ccgaacgtag cggtaagagt ctccagaaac
     3481 ttttgtatgg gtcgcgagat gcggtcgaat acctcgtatc cagaagccca tagcgtatct
     3541 attgcgggaa ggtcagccac aactaatcta tatgcaggtg aggaattact tgccgcctcc
     3601 tgacgcaggc ggttgcgtta ttctgagcaa ggcgtagtcg cttgggttag gttcgtagct
     3661 aatgtcgttg tgccacaatc cgcgacagct ttggcgtgct gggagtgccg gatcaaatgg
     3721 tgtccctgct aagagtttct tgttctcttc agaattgatc cacaggattt caggatccgc
     3781 gttgggatcg ggctgatgag catggcggtg gagagaggag gtggctggct tgccactgag
     3841 gactcctaac ttttggacga tggccttctg catttcttca gtgagcccgt cttgccgacg
     3901 aaaaaagacg acgccgcgac gtgagactac ttttgcgatt agcgactggg aagcttagtc
     3961 atcctcaaac ctgatgtgga tgccatatat ctggaagctg acctgttatt gctaattcac
     4021 gaagcagatc atctgccttc ggatcttcca accaggcttt aaggcttgca tccggaaact
     4081 ctgttccaat tattggtgta acatcgaacg aacggaattc gtccaacgcc ccagttggtt
     4141 tcaatggctc tttgatgaga gccatagtgt accaatagtt cggaggctct cgatcggaat
     4201 aagatagaac aatcgcaaac acggctcaat cgatcaatga cacgtgtttt gtagaagctt
     4261 caagagacag aagttgggaa gttccagcgg agctaccgat atactcttct taagtgtcac
     4321 agtcggtata ctgcaagtga tgcgggcctt ttcgtccgac agaactgtat gcagcatgat
     4381 acgttcattt tacccactaa gcagagtggt ttcaaaacta ctcaacaaat taacacgttg
     4441 tttcggaccc ctctgtcccg tcggacacgg gaagccgccg tatatcaaac gccagtgacg
     4501 tggcaaatct tgggtgagat gcggagttca atagttcaag tctcatcggc ggtattacgg
     4561 taagttataa gacgcccttg cctgcagcac ttttcggcgt aaatatatgc gggctgaggc
     4621 atattcggcg atgatgactg caccgcagtc gtccagccaa gtcgatccta tgtaccgtat
     4681 gcacagctaa aaccgggcct gttcgcgaca tgatctacct atgattattg ccctattcac
     4741 atttaccaca aattccatct cctcattctc atcatcgtca acaataacgc gccatcatgt
     4801 tccacactat caatatcgtc actccggctg agggaagaca gcaaagggta atatggatca
     4861 ttcgattctt aatttgaacc tgcaataact gacttgatgt tattatctgg ctttcaggtt
     4921 cgggaaatgc tcgatcatct tgtctccgaa gttcaaaagc acgagccgaa tgcgatctcg
     4981 tttcgagctg tttgggatgc ggaggccgga gtgttttatg tgatcgagaa gtatgttgat
     5041 gtagtctctg gttcatgatt tgaagatatt aacggttgac agatttaccg atgaggaagc
     5101 ttggaaacac catcgcgacc aaccgtacac ggcagagctg cgccgcgtat cgcgggaaga
     5161 acacaacctc gccaagaaag tggaggtgcg tcttgtggag agctttgcag aatttgcaag
     5221 atagataatt ttaagcaaac atggaattgg atatctcccc tgcaatgatg tttcgatgtg
     5281 cgtgtgtaca tgtgagagat gcgagtataa gagttgtcgg atgtaccagt cttgtctatc
     5341 ctggttgccg ctcatgaatg acgcgtgaat gaaactcgtt ggacaaaatg gtagtgcaac
     5401 aatgagattt ggcaagtcaa gagaaaggtc aagtgaagaa tattcagttt gtatgcataa
     5461 aaatcaagtg ttttgcatca tgtctctcgc agtgtctagt aagctttgct ctcatcctca
     5521 tagctcgccc gtcccctctt ctctctcttc ccattcagca acccatgctt tcactgcctc
     5581 aatctctttc tcatgaacga gtcgaatctc agcgggctca tcaacaagaa caggcaaagc
     5641 cgaacgatcg ttcgcgaaac ctccttgccg taaaacatga ccatcgccta gtttcacgcg
     5701 catatcgccg gccaagggag aatgtgcagt gacgtgcagc cacgggtcat cctcaccgtt
     5761 gacaaagagt aaacgacggt cgtgaatact tacaccgcca tatttgttta tacggtcaac
     5821 ggtgacgaga tagtcatcag ctacagaaat tagtaagctg cggagtcatg gtggtgtgga
     5881 aaaaagcaaa acttacggaa gtcgaaagca aagcgacaaa cttcagtcaa gtaatattcg
     5941 ctgagagagc gtgagagaac cgctggcaaa ggatatggct cagctgcaga gaaataaccc
     6001 cactgtggac aaacagtcag tgtctagtct ggcttaggaa gactggaagt aagattcata
     6061 cctcagtgca tgcttgccaa taccataagc gccagacttg gcccagatca gtcttctgag
     6121 aatctgtcct gttccgacga atctctccaa gggtgaaaaa cctgtcgtcc gaggcttggg
     6181 agagttgtgg tatgttttgc tggtggccta cagccaggta gtgacgaatg tagcgagcat
     6241 agttctccat ggctcggctg tggaagactt ctctgatctg ttggtcagat ttggtcatct
     6301 tgttgttgct tgtttcgtcc agctgaattc cttgcgtcag gttcgcgcag aaatactccc
     6361 acgcgcgatt gtcctgctga cgatcatccc aagatttcct ctgccatagg cctaggggtg
     6421 ttgcgacctg attcatgaag tcgcgcactg tcttttcgtt gctcaaattg aacatatcac
     6481 gataacgtgc tagaggctcg gtaatgatga tctccccatc cttgtcaagg ccggctcgat
     6541 caatctcatc atcgatatcg gccacgacag tcgtaacaac ctgcatgcag agagggtctg
     6601 ctcgcagcct aatagcttcc aagtactgcc acacgttttg gatagccaac agaggagctg
     6661 aggacgcaat agcaccccaa agctgctttg ggtacatctt gcgtagtaag gcacccttcg
     6721 tgccggcgat ccagtggccg taagatatcc aaggggcatt ggcgggtagt atagatgtat
     6781 ccaattcgtg gtgttgagca tgggggaaat tctcggcaaa gtgggccatg tccgcaagag
     6841 cttcatccat attcaagaat tgaagactgg cggtagacag gttttgggtg acaaaagagc
     6901 ggccatagta acgttgctcg aggaccacaa cagcagcttt cacatcgtga gcaatgtgac
     6961 tgacgatgcc ttgctcaatc aagcgaactg tctcctctcc tggcttatcc gctccatcga
     7021 caatcaggac aggatgacct ttcgagtatt tatccaagtc aagccagtat cgttgctcga
     7081 atgtggcatt aaagcctgcc ggaagagaag tagccaagtg ggttgtccgc tgagtaaagc
     7141 tgaagggtcc ttgaaatgag gcatgacatg tagcaacgaa gtaatgggtg agaatcgttg
     7201 caggcttcat tttgccggat ctatcgggag tgtggagatc cggaatatga agtctgcaaa
     7261 gcaaagcggt agtagatatg aagaagtgtt ccttaaggct tgaattgtat agatctttcg
     7321 atcctcaagc aaagtcttgg cggcactttg cgattcggtg tcaacatgaa tctccatagt
     7381 cagcggtgac gctaccgtga gatctcctag ccgtgctgcc agctcgagcc atgagctggt
     7441 ctcagttcag accgccaatc gatgaggggt acggcaggct tgcggttcag tgcacaatca
     7501 gtcttcggac cgaatcttct cggccgacga catgaactgt gaactagtcg gtagcttttg
     7561 ctatccgcgg gaaagatgct gatattaggt agtcaacgga agcacatatg caagttcatc
     7621 tatttcccat gtcagatcca ccttccggga tgtgctgtca tacagctgca ccgagtagaa
     7681 gacagagact actgacatgt agcgtgcaaa gttacgtggc attttctcca taaagtttcg
     7741 gcgatccaag gatgctacta gtagatacag aatgcgcgaa tttccaaact ttgccgatct
     7801 ggcaggttct cagtgccagg cataccaaag gcaccgtggc cacacctcca cgctgtattc
     7861 tccatttttg agtcgtggtg tttgaacacc agcagctcgt tcagacgctg gttggacatg
     7921 taataccact ggtgatcgac actaccctcc ccgtgcttca acaccgacca ttcaccgtcg
     7981 taagtctttc tcttgttagg ctgcaggata acgtaatcct catctgggac ggtacgggag
     8041 tctgagacac agagcgggtc cttctctacg gccttgtaaa gaggccgcca aagacttgat
     8101 cgacatggag ttagcgtgtg tgatcttgag caagttgacg ggacagacgg atgtggcgct
     8161 actcacccta gaatcgaaaa gcgtggcgct ttttcggcgt ctgacagatc tacgccaatc
     8221 gactctagcc acttggggac agcggcccgg agttcagggt cggtgaacgt tttccaccaa
     8281 ccctggctgg ccgaattagg accgaaatct aaatggggcc gtggaatggt cttctgcgtg
     8341 ttaggaatag gtttgtctga caggtctagc ggtctcaaca ccggcgcgaa gcagaagaca
     8401 tgcttgacgc cgatctgctc ttgcaacagt tgctcgactt ccttgtagta cttggtctgt
     8461 atctccttgg tatctttgtc attgcgctcc cggtacggaa gatggaggac ttcgaatcca
     8521 tgagtgtcga gcttgtattc gtccttcttg ggccgcatgt ctcgtacaag cttggtgcgc
     8581 ggtgctcgca gactggtatc cgcgactacc tcatcggaac agacccgctc gggatccttc
     8641 aagtacgaga tctccgtgtg gaaatcaatg ggggaaagct tttccgacat gattcgttgc
     8701 aaagaatggt agtgcgtaat atttagatgc agaaatgggt agatgggata tgatagcagt
     8761 ggagtatgag aaggactcaa cacttcactt ccgcacctct atatatcgtt gcccgccgat
     8821 agaagcgggg actgtaagcg gcccgaatac atccgtctct ggcaactaag cggaagaaat
     8881 taagcggaca gattgggcgt tttaacacac tctattattg tgacagaaaa catgccactg
     8941 tcgggttcaa atacaggtga tggcaagaaa gtagagtagt atccaactag gtggctgtgc
     9001 tggaatgaat gcctcaaatg caagactatt tggttatgtt ggtatatagt cactagccac
     9061 ggggaagtat gatgatatct ttcagtgact caatggaagc gtaaagccgt tggtgaatcg
     9121 ttttggtttt gttggaaaga gattaaacag aagcttcaac tcatgctctg ctacgctcca
     9181 ggagaagctg cggcaagccc acctacaggc ttgaaagaga ccaagattgg tggcatctcc
     9241 cagagcagct tcaatctggc cacatccatc aagtcctgcg tctcgcctat aatttcccag
     9301 tcaaatttca aaaccagctt tgctagaaca attctccatt cagcataggc aagtgacatg
     9361 ccaatacact gccgggggcc tagagagaag ggttttgatg cttctttgcg gtcccctgaa
     9421 aactgttcgt cgtagaattc atgatccttg ggtagccatc tctcaggaag aaactcgtaa
     9481 ggccgcacaa agtatcgcga gttccgtacc atgaggatat ctggcgcttg cactacggtc
     9541 tggattgcgt ctcacatcag ctgtgtacga tatgcaatgt atggggaaaa tgtgcaactc
     9601 actcctttcg ggacgtaaac gcctccgatc attgcaccag ggcataccct tggcagcccg
     9661 aatcctacag gagggaagat gcgaagggac tcgtcaatta ccgcttgaag gtacttgagc
     9721 tgcttggtag attcgatatc gatctctccg ctctgggaga acgcatttct tacttcgtat
     9781 tgtagtcgtg ccaaatgtgc aggatgcgca aggaggttgt aaataaggga agatagtaaa
     9841 gtagaagagg tttccgttcc gcccgacacg agagcagctg cctgaccttg tagaaacact
     9901 agatagccat gattgtcggc gtctttctca acgggagggc atgtggcaag gacgtggtcg
     9961 aagaggtttg cggccttggg cgggtcacgg agtctttcct cacacatctt tcgacagacg
    10021 gacagatact gagcccttaa cttcgccgtc ttggatagct tagcaaatat cgacctcagc
    10081 aatgtcttga cagcatgtgg ctgtcgacga attgcgacac ccaccgctgc cgccgcaatg
    10141 ttgcctgtta agagatctag ccagactgtg tttttgcctt gagcgacagt tcctgacgat
    10201 ttcccaaagg tgacctcagt aataacgtcg aaggtaaaac gagccgccca ggctttcaca
    10261 tcaactgcat tggaatgttc cgacgccttc tcaagctgcg ctgtaaggtc atcggccact
    10321 ctctcaatta cgcgcccata atctttaagc gagcttggac taaatcctcg ctcaagaggc
    10381 tttcgcaggg ttcgatgagc ggctggatca gtctcagtca cgagagttgg gaatccttca
    10441 ctctgcttgt agaagggagg ctgcttgatg aactgaagat cctggtcagg attacagcgt
    10501 gtgtaaatat cttgaagcac ttcatgttag caagcaacat gacagtgagt atactgcttg
    10561 gctagtacaa tacctcttgc tgcctcagga gttgcgaaga ctagctcatt tggggcaatt
    10621 cggatgacgt cgccatattg gagctgcagc ttctccaggg tctgatggta tcgaccattg
    10681 agcctaagat gtcttagctt ctcgaatcca ttacggattg gatcacatgc tccacatacc
    10741 attttttgca aaaccaccaa tcagtgacag cggctagttt cggtcctttg aaggatgcca
    10801 aaggatgcca aagaaggcgg tagacggcaa ttcccatgag aagagggaag aactagtgtc
    10861 gtgagcaaat tgttagagca ctgacagaaa gagtacatac cgatacaaca aagatcaccc
    10921 ctggaaatga catagtgaac tgcctcgatg gttgaaactt ggtgcacaag ttacggtatt
    10981 gactcctgga acatagcccg gcgacacgca cccataatga aggctagtta gaagttttcc
    11041 ggtcaatcca catcgaactc tgacatcatc ttgatttttc ggcctctctt ttggcgctct
    11101 ccggtagacc gccacgtagc ccctcctttc ggcccggacc cgcggagctt tgacagaaca
    11161 ttgtgttgag acagagcaat ggtggaattg aactccgaaa gcatcaccat ctccggcgcg
    11221 tatgtaggtt gctttagcgt tggaccactg tgctgcaatc tgcatctttc ggccaatttg
    11281 tctcggcaat cttcgggaga ttgccacgta atccttcgtt cgtctaggct cgggccgcga
    11341 ggctttgaca tgaacgtgtt atcgagtcag tacaaaggtt gcgttgccta tcttgtaagc
    11401 aaggttttgc ggacatagat ggttctaagt gctgatggag tgcggaatgt cttacgtggc
    11461 acgttgttag atgaggagta tccaacaaga tcctagttgt caccaagaac gggtgcaatt
    11521 tcgcaagcag tcggaaggcg cggaaccgtt gctgcatcac aggtttgaaa cacgatctag
    11581 agtgtcgttg aatcttgcat acttggctag tcatacactg cagtgtacat tatcaggctt
    11641 tactacttat ggaaagagat aaccaacacc cctgctagca ggataagttc cccgcaacta
    11701 cagttgctgc acgtactagc tctcccgctt ctgcaccttg acaactacct tgtgaccaag
    11761 ccaaagatag gatgaatcgc aatctctttc ccacacaaaa tccctgttta ccatacgcag
    11821 gtcgtatgag aacactaagc gtgcaaggac cagccgcacg atctgcaagg cgagctcgcg
    11881 cccgagacag gctcttggac ccaatagaaa cggccgatag gccgaatgca cgtcgttctc
    11941 gaactcagat tctggctcac caacgctagc ccatcgttcc ggccgatacg tctcagggtc
    12001 tcgccagtac ttcggtgaac gctggatggt gtactgatcc accgcaaccg tgatacctgc
    12061 cggaaggtag atgccctcta caaaacctcc attggaaagt cgatgtgtgc ctgtaccgtt
    12121 cgctggagag agcagacgca tggcctcgtt gatggagcca ttgagaaggg gaagtctagc
    12181 gattgctaga ccgttaatct cgtcttcgga tttgaactgc cctcgcacct cgtcctgcag
    12241 attggccata tgttgtgggt ctcggagcaa gaagtagagc gttgacgaga gacatgtgac
    12301 agttgcatct tggccaacca tgacgagcat ggcggaatta ctctgcagct cgtcctgaga
    12361 gaggacaata cctttttctt gggcgccaac gatggcacta aggaagtcat ggccgcggtc
    12421 tgcgcgctcc agtgttctgc gtaccttgcg ttagcgggag tgttggaatg aaattttatc
    12481 tgcggatact tacgtgcgca gggtaccagt tggcaatatg tcgcgaggca gctgaaagac
    12541 ctgcggaaat gaggtgacga tccatctcgt gaaggctccc gcaattggga atgtgaggat
    12601 gctctgcatg acctccaaca tagggctcat agcgcaagca agccacttga gcctgttgaa
    12661 tttgactttt cattccgtta gcctccctca catttcgact tctttcctgc gaagatgact
    12721 taccaagttg gcttcttgcc aaaggctctc cgaacgagag atcactcaac atgtcccaga
    12781 caaggtgcga catatgttca gtaatattga cctccgtgtt atctacagcc gcagcatcca
    12841 gaagccgatt gaacttggta atccattctt gttcgatggg ctcctgcttt ctcagctcct
    12901 tggccgaaaa ggcgggttgc accgctttgc gaagtctggc atgccctttg acatcagtct
    12961 tggtcaagat gttctgtgca ggccatgtca tgaggccaag aagccactcg atggtgcctt
    13021 ctttggtgaa agtaccgtcg tcgccaccag ccttttcgtg atgcactgag gagagagctc
    13081 caactgtggc gaatgagacg gtgttgggac caacgcgaac cacatcgcct ttaaaccata
    13141 gtaagtatcc agctcacaag aaggcgaagt cataaatatg gctatacata ccataccgat
    13201 catgtagcgc tttaatatct cggttcagtt tcttgccata caaggatttc atcatccata
    13261 ctggtgatat tgccgccagc tttggccctg gtatctttga gagagggtgg aagaagaggt
    13321 agtagataca cgaggatacg agctaaaaca ataatcttag atttgatgta cgcgttgtat
    13381 tcttgcagaa ctcacaacta gaggcccaaa aatcagcaat agtgatggtc ccatgtcctg
    13441 tacaagacga ggcatcacat ggtaggcaag ctgtagtgta ttgccattcg gcaaaccatc
    13501 agattggacg tctgacccgt tcattgcatc catgtgctca gagagggaat tgggaatatc
    13561 gtaaggtgag attgatctct gtacgccttc gcaaatgaga ggtaacgaca gcgtgcttgg
    13621 acagcttgtt ccgaacgtcc gcttactccg tacccagacg cgtactgatg tgtttcgcca
    13681 ggcggggtac atttaaccca gtttcaatag tttaacaaag tggcatgtgg gtaatatcag
    13741 tcgggcatcc atgacccgct gtgagcggcc cgctcggtca gactacgaaa gcggatcata
    13801 tcaaagatac tccattcgaa ctcgacttcg cggatcatgc ggcataactg cagtatacgc
    13861 gttctcgtac gcgctgcgta atcttagtat tggcgatagt agcagctggg gccacccccg
    13921 cctagtatcc gcctttccac caacttatca acacattcac gcattagatt cccatctcag
    13981 atagtcgccg atgcaacaac gggatcatcc actgtggaac gatgatccat gacaagtgga
    14041 ggtcgattcc gaaagacctc tatcggacct ttggtggggc aagttaaatc gtggatagaa
    14101 ctggattacc atcggttaga tgccagagag catctctaga cagttgctag tgtgatgttc
    14161 ccgctttcca ccgtagaaat gccgtgaatc tcgccgtcgt gctcggccta cgttcaccgc
    14221 gtaagccgat ggtaatcccc atctacagca tatgtatgta ggtttctggc cgtgaggaaa
    14281 gtctcatagc ggttttcaaa ccctggagag tgtttttgtt tgactaaagc tgaatttgaa
    14341 actcaacgcc gatactcttt ctcgtgcttt acgctggaca ttggccaaaa cccagtgaca
    14401 atcctcagtt acgactggta tcattacttc tcacgaaatc caactttcat cacttcctca
    14461 cccccaaacc tgacctatag ttccgcaaga gtcatggcaa accctccagt caccgagtgg
    14521 aagcagtccg aggcaattcc agcacatgtc gcccaggaga cgggctgctt caccacgctc
    14581 cccatccgga tccacaaaaa caactcgata gcagaccaag caaccctcga atcaatcaat
    14641 gactggaaac aacacttgga cgacggctgg gaatccagat ctggttctgc aatttccaaa
    14701 gtagggaact ggtgctcgtt tatcttctct gaagcgctgc cggagagatt accgtgtatt
    14761 acgtatctgg cgaatattgg gaatatacat gatggtgaga ttacctgtga attaaatgac
    14821 tatagggttt ttgtttattg aactgacgac tgttgggtag atgcaaccga agacgccaca
    14881 ctagacgaag ccataacctc acacgcacaa ttcagtaccg cattatcact cgacaccgac
    14941 gatgaaccat cccttcaaga agccaaaacc agaagattcc gccatctcgt gagtaattgt
    15001 gtgcttgaga tattgagcat agatcgagat atgggaacga ggatgatagt gtcgtatcag
    15061 aagaaatggc tggatgtgat ggaacatttg aattatgagg ggattgagag tttggaagag
    15121 tatttggagt ttaggatgtt gaatgggggg atggagtaag tcccctttta ccattacgac
    15181 gaccttatag ctgctttgca acaccgcaca tctcatttgg aaagagctct accgagttct
    15241 atttctcctt cccccctttt accacttttc tcaaggctcg tggctaacgc ggacggcaga
    15301 ccattctggc tgatgtgcca atttggcatg ggactcaaca ttccagacgc ggaactcgca
    15361 ccaacaagac acattttcga gcccgcggaa tgggctcttg ttctcacgaa tgattactgg
    15421 agttggggac gcgagtacaa tgcgtcactc acgaaaggct caaggatcgt gaattcgatc
    15481 gagctcctct cgcggctgcg gaacatcagc tacgacgagg cgaaagaggt tgttcgggac
    15541 ctcataacca cgtacgaagc ggaatacgaa agaagagttc aggacttcct gcgtgaaaat
    15601 ccatccacgc agatgtacct gcgtcagttc atcgaagtcg ccgggctggt agtagcaggt
    15661 aaccattact ggtgcgccaa ctgtccgcgc catcatgcgt ggaaggagca ggatcaagcg
    15721 gtacatgcag tggaccaact ggaagaattc cccgctgttg aagctcagtc cccctcatcc
    15781 gcaacacata cagcagcccc acaggaagac cctccagtca ccgaagcccc gtcccccatc
    15841 tcctcaccta gctcctcctc atccgccaaa ccctcctctt cctccgccgc agactcatca
    15901 agctgtacat ccacctcaca gcactctccc agcgaaacag acagcacccc gccatcctct
    15961 ctccacctcc tcaaatctcc ccaccccgtc gacgcaccat gcacctacat gtcatccctc
    16021 ccctcaaaag gcgtccgttc aaccctcatc cacgccttaa accaatggtt ccaaatctcc
    16081 tcccgaaacg tcaccttgat caaagaaata acaagtatgc tgcacaactc ctctttgatc
    16141 ctggatgaca tccaagacca atccccgctc cggcgtggaa aacccgctgc acacactatc
    16201 ttcagtaccg cacagtcgat aaatagctcc acatacctgt tcgtccgcgc catgcagatg
    16261 gtgagcgaga acttcgagtc gagcgttcag atgtcctttt tagagatatt gcaacgtatg
    16321 catataggac aaagctacga cttacactgg cggttccacc tgcaatgtcc cacagaggat
    16381 gagtacttcg agatggtaga tgcgaagacc gggtgtatgt ttgaaatgct gttattactc
    16441 atgtcgtcga aatctcggca cgtcaaagat catagtttta catctttcat ccggacattt
    16501 ggacggtact tccaggtacg agacgactac atgaacttga catccggaga atacacagcg
    16561 gggaaaggat ggtgcgagga tctcgacgaa gggaaacttt cgtatccgtt gattgaatgc
    16621 cagaaaatgg cccctgcgtc ttttacgcag attatgggga ttttcagggc gaaacaggcg
    16681 gatacggcgc aattgtcgga tgagacgaaa aagtatgtta ttgagttgtt taggaagtcg
    16741 ggggtgttgg atagtacgct tgattggatc atggagatgg agaggcagtt gttgatggag
    16801 gtcaagaggt tagagggggt gatgggtgat tcaaacccga tgctttatgt tttgctgcag
    16861 acgcttagct tgaaccaatg atattttgtt gaagcttttt gctggaatta tgttcgcgct
    16921 gagagtggac gtacctgtga atacctaggc atggcacatg acaagtgcta ttgggttggc
    16981 cggactgacc aaggatttat gctgtgcgat tcttcctacc atgttgaagg tcaagtttga
    17041 tggtaatacg cagaaagtga tgaagatgat gtcaaaaaca gcggattggg ttggagaagg
    17101 cagaaagacg gtaaaagcga cggccacctt gcgctgacaa cgaggatatg ggtgggactg
    17161 agacagataa agtgtatatg cagaatcaaa tagggtatac caaaacatga actcctttcc
    17221 aaactccttg aatgcgtgtt cgaccatacg aattattctc gccattcttt cgcgcatttg
    17281 gtgcactggg ttatgtcagc aaagtctgga tgtaggtttg gaatcttgac caaccttgta
    17341 aaaagcagtc ataggctcat cagcactacg aatctgcaac tggtaccagt acgcctcgtg
    17401 gtttctgcac ttttcgttcg gacattgcac tgttttgttg tcagtcacat ggccctggac
    17461 ttctctttca cttggaccct ctttctagca aacgcacctt cagtcttatc cacgttgtcc
    17521 catgcgcctt tgcctcctag gatatcctcg acttccttcc tcttgatggt cttgcgctcg
    17581 tagtagcgct tgtcgatgac gtagtggtaa ggacatgtga ggcattcgaa acggttctgg
    17641 ccgactgagt cttcggttgt gggatcaccg gcgttgactt tggagacgcg gagcatattg
    17701 ctgcatgttg ggcagactgg gagggttagc cttattcttg gcgtatagaa tgatgatgaa
    17761 tatagcatgc agtatggtaa ataatatcta acttacataa caacatggtg ggcgacggtt
    17821 gggcaacttg caaggaagac gcgtgaggcg gatgaatctt gaagatgccc aaaagttacc
    17881 gcaggaccct gagaagctag cgccgcatca ggcaaagcgc ggggcggcga cgaataccag
    17941 cgctgagcct ccggccaacg cggacgctgt gaaggggctg tctcaggttg cgggcttatg
    18001 cgcgctggtc tagggacttt gctactgagt cgctcttctc gtgcgtgctc tccaacgtca
    18061 tcacgccctc ttcacaggat tctgaggcca cctcctttcc gttagcactt ccgagtaccc
    18121 cgccaaacaa caccgccgaa acattcacgc gcacctggtc gaagcgaagc gattaggcac
    18181 cctctcctcc agcacgctca ggatatcccc ggacggcccc aacatgtccg acgacgaggg
    18241 agcctctccc cgcgagcgca tcctcgctgc ctgccgcagt gggaacaccg acctccttga
    18301 agaggctatt gcagatctca ccaccgaagc ctccaagtcg ggaaagaagc cggccgagca
    18361 cgtcgcggcg gcactgaacg gcgcacgaga cggcataggc aacgggtgtc tccacctcgc
    18421 tgcactgaat ggaaggtgta agtaggcacg agactcatat ggcctctgac gagtgtccac
    18481 gtcacgttct gagatcaggt cacaacaagc acaaccaact aacacccccc cagacgaagt
    18541 cctcgacgaa ttgctcgacc aggagggcct cgagatcgac gagatcgacc gcctggagca
    18601 agataccccc cttcacaaag ccgtccgcta cgtcaacacc ctcgacccgt ctgaatgggt
    18661 gtcgttcggc ctgcccatcg tcgacatcct cctagacgcc ggctgcgacc ccagaatacg
    18721 gaacaaggcg aagctcaagc ccattgagct ggcggacccg aggaataccg agctgaggaa
    18781 gatgttgcaa aagggagagt ttgccatgca ggcaggtgca gatgtagtcc acgaggacga
    18841 tgacgggcca acgggcagtg gcagtgatag tgattagatt gtcacgttgg agggaggaga
    18901 gtgttctgga agtgcattgg accctggtta tggggtgacc ggagtgtctt cattcaaatg
    18961 tacatgctgt tgcccgccag accactcagc atttgtggcg gataatatac actttggaca
    19021 gtctagtcct gcttgccttt cctactctca tggatatcgt ctaacctctc agtcccagca
    19081 ttttttgtct cgggtgtcca tctgtttccg ctgtaagcac cgcacccccc gccagtgttc
    19141 tatgtttgcc ttcttgaatt atatgcctcg cttcgtctcg tcaaatattc gggtatgctg
    19201 ttcgttcaat cttgttgctg tttggtgtca gccgttgctc tcgatacccg ccctctgcta
    19261 cacatgacga agactgtatg tttgggaaca gaatcgtatg tactgaaaac cattacatcc
    19321 cctcccctca aacatcactc acctcaggcg gcggtcccac aggccgaacc atgcgctcca
    19381 tcaactgtag ccttgtcttc tcgcgcggct ggccaagacc aatgctcatg tactgcagta
    19441 ggggcgggtg gccgacgtag ctcgacagcg agtcgcgcgc gatgttggaa gtccactcgt
    19501 gtttggtcgt gtctgcgctg ccgataccga tgtagcgcgc ctggagggct tcgagctgtt
    19561 gctgcgcgcg gagtttgtcg gccatgatgc gatgacgata tgtgcgggtt tcagtggatc
    19621 ggggatagta tgagatgtat cgggagtatg cggcggtagt taaggcgcaa aagttgggga
    19681 agtcgcgatg cgttgcggag aagtcgcgga aatcctgctt agtcattcca tctccgacac
    19741 atgctccatc atcatcgcga cagcttcgcg accgaactaa caacccggca aacctccaat
    19801 caaaacactt tcttcatttc cgcgcttcaa agaatacaca actaaccatg ggcaacgaag
    19861 acataatgat gagcaacgcg ccgctcactc cgcgcgacga acccgtcgac ctcttaacat
    19921 cccccagcgt cgacttctct ccctctacag tccaatacga cgaaactttc gaaaacaacc
    19981 tcatggaaat catcctaaac ccatcatcta catccgatca gcgcatacca acaccctcgc
    20041 gcgagccccc catggtttcc gcctcacaac ttcccatccc catctcctct cccgtgcgaa
    20101 cgcacccctc ccccatacca ggtctatacg tcacacacgc aaacgggtat cacacgggcg
    20161 gacccggtcc ctcaccccac accatccaag aattcgcaga ccgcttcata cgggagcaca
    20221 gtattgagga tgcgggacaa ctggagcgcg tggtggagga gattgtagga caaaagatgc
    20281 aggaggtacg ggagaggatg gagaggagaa aggaggttgt agagagaaat agaggggtgg
    20341 aaagggagtt ggaggacttg aggttgcaga gagcggcgga gatgcgcgtt atggagaggg
    20401 ttaaggggaa gaggacgtga gatgttgctt ggattaccta ggcggtgctt tgggggaggg
    20461 gatgtggtgg atatgatacc cgacattgtt tttgtcactt ttgaggatga gagggggaga
    20521 tgtattgtca tatgtgggaa tagagtagat ggctatatgg ttattgtatt gtatacgtac
    20581 tatctgtaca cgcgaacaca tgcccattac agaccgtagg atttgctgca acaaaatgtt
    20641 caaaagcgac aatccaatcg caatgaacac caagatacac catcacatgt acaaacatac
    20701 actaccgtag acctgagtaa ttgcatgcat atataatccc aaatgcacat gagcggcagg
    20761 caagcggggg gaggcatgga cagctctccc gcccgcctca gcgcaccgac caatagcgtc
    20821 ataaccaccc cgatcgccaa agatcgcaac ctatcgtccg ccctagaccc tggatcgaca
    20881 cgccctgccg cgcgggaaga aaggtcatct gctatcttcc tcttgtccca tttcctatct
    20941 tctggctgat cgcaatctgc gatcgagatg ctgtgcttgt tccagcgggc tgcaggtgcg
//
