LOCUS       BGC0001529             12821 bp    DNA     linear   BCT 06-JAN-2018
DEFINITION  Streptomyces sp. RI18 DNA, bez gene cluster for biosynthesis of
            bezastatin derivatives, strain: RI18.
ACCESSION   BGC0001529
VERSION     BGC0001529
KEYWORDS    .
SOURCE      Streptomyces sp. RI18
  ORGANISM  Streptomyces sp. RI18
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 02:21:17
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12821
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..12821
                     /db_xref="taxon:1894972"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces sp. RI18"
                     /strain="RI18"
     subregion       1..12821
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Alkaloid"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12821
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(220..1173)
                     /gene="bezJ"
     CDS             complement(220..1173)
                     /codon_start=1
                     /gene="bezJ"
                     /product="N-oxygenase"
                     /protein_id="BBC27531.1"
                     /transl_table=11
                     /translation="MTVAREEAAYERDLLAQLTRRWGKRVAVKKDELDLDGHFDEALPD
                     FPEHLVPVLDLPGADRLDREARERILSAAWISYNAKTAAIEDEIILPACRLMLQERVPV
                     RRDDVAVDALHQTIIDEHYHILMCHNAVGVTRRRRDLENLRFAPDVWSVVRGRNEVRTG
                     LTGFDRDIVDIAFSLAAETTISGFLSTLATAREIQPMNRITTDLHRRDEGGHAVVFREL
                     CGSLYRNLDGTRQRMFREALVTGLTAFRSADLDPWVTVAAQGGFEIDTGQLSAWAAARP
                     APARDTGPLRLLLDDLGIDHDLVEPVHHRPPRPTRP"
     misc_feature    220..12060
                     /note="bez gene cluster for biosynthesis of bezastatin
                     derivatives"
     gene            complement(1157..2155)
                     /gene="bezI"
     CDS             complement(1157..2155)
                     /NRPS_PKS="Domain: Aminotran_4 (94-288). E-value: 1.7e-26.
                     Score: 85.3. Matches aSDomain:
                     nrpspksdomains_bezI_Aminotran_4.1"
                     /codon_start=1
                     /gene="bezI"
                     /product="PABA synthase"
                     /protein_id="BBC27532.1"
                     /transl_table=11
                     /translation="MNTTTTDGTTDGTTDAAMGTTAGTTAEARDGTTDATTGAMAEARD
                     AATDAATDAETVQGVRRWWDADGGWSTADSTGTVLVIDSWLVDEGRVRGLDRHAARFAA
                     ACDRFRGPGAERTDRFLRAVAEASPARGRWFPRVELVETGSGTRLRMWPRPAPPRTATV
                     RLWTAPGPDRRRLPAVKGVDLDHLASLRAAATGAGADEALLVSAEGHVVEGATTSVVWW
                     RGDTLCGPPPGPGLLPGITRALLGELAAAAGHQVVTERATPQDLADLPVWTLNALHGIR
                     PVTEGLGRFRGADTAATELWQSRLTALAVPADAAPLPPAYPRRRFPDDRGT"
     aSDomain        complement(1292..1873)
                     /aSDomain="Aminotran_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_bezI_Aminotran_4.1"
                     /evalue="1.70E-26"
                     /label="bezI_Aminotran_4.1"
                     /locus_tag="bezI"
                     /protein_end="288"
                     /protein_start="94"
                     /score="85.3"
                     /tool="antismash"
                     /translation="LDRHAARFAAACDRFRGPGAERTDRFLRAVAEASPARGRWFPRVE
                     LVETGSGTRLRMWPRPAPPRTATVRLWTAPGPDRRRLPAVKGVDLDHLASLRAAATGAG
                     ADEALLVSAEGHVVEGATTSVVWWRGDTLCGPPPGPGLLPGITRALLGELAAAAGHQVV
                     TERATPQDLADLPVWTLNALHGIRPVTEGLG"
     gene            complement(2152..4341)
                     /gene="bezH"
     CDS             complement(2152..4341)
                     /codon_start=1
                     /gene="bezH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1018:isochorismate synthase (Score: 396.6; E-value:
                     3.1e-120)"
                     /gene_kind="biosynthetic-additional"
                     /product="PABA synthase"
                     /protein_id="BBC27533.1"
                     /transl_table=11
                     /translation="MRTLLLDNYDSYTFNLYQLIAGINGREPVVMVNDDPSLAGLPLAD
                     FDNIVVSPGPGRPQNPRDVGLLGDLLRRATLPVLGVCFGHQMIAHLAGAPVVAAPEPRH
                     GHLAKVSHDGDPLFAGVPREFVAVRYHSLCVPEPLPEELVATAWADDGVVMALRHRELP
                     QWGVQFHPESIASQYGPEILRNFADLTRGTRHGQRPSVTVTGTPTRASADTGTALAEEM
                     PGTVAETLAEAEQMPGAAQELPGAAGGLGIISAVVPTAADSEAVFLRLFDNLPYCFWLD
                     SSRVEEGLSRFSFLGDTSGPLSEVLTYRTGSGAVEVRDAHGVHLVTGSVFDVLERRLRE
                     RRVPDPDLPFDLTGGYVGYLGYELKAECGATARHTAETPDAVWMFADRVVAVDHQEGLT
                     YLVAVHDERTRRAAQEWVDRTSALLTDLPPADSGPSYAPAAGPLSDAGDHLGRDRDQYL
                     ADIAECRRQLNQGESYEICLTDMLHLPFHDDDTSFYLRLRRANPAPYAAFLRLGGITVF
                     GSSPERFLRIERDRTVTSKPIKGTAPRDADPVRDAELAATLASSAKTQAENLMIVDLLR
                     NDLGRVCEVGSIEVDPYLAVESYETVHQLVSTVRGRLKDGVGAVDCVRQCFPGGSMTGA
                     PKLRTMEIIDRLETEARGVYSGALGYFGLSGGTDLNIVIRTAVRVGDRLSIGAGGAIVL
                     DSDPQEEYEEMLLKAAASLRAWRAPTVSDVGGGPR"
     gene            complement(4341..5189)
                     /gene="bezG"
     CDS             complement(4341..5189)
                     /codon_start=1
                     /gene="bezG"
                     /product="N-acetyltransferase"
                     /protein_id="BBC27534.1"
                     /transl_table=11
                     /translation="MNDEPRVTDSMWQGSGVDLDAYLTRVGYQGDVAPDLATLRGLHLA
                     HVDAIAFDNLDALLGRTAVPLDLESVQEKIVRQGRGGWCLEQVVLTAAVLDRIGFTFTA
                     FAGRTRIRTGSRFGPALHVALCVEIDGERWLHDVSFGAHGLHEPIRLADGARLDGDWPY
                     DLVREPTGEHVLRFLRPEGPVELYGFTTDPRYPSDFELLNHFCLTHPRSPFNHRMVLQR
                     TQPDVRHLLAGSTLMEIRPAEPVTSRELDLGELLSAPEEMFGITLEPGDVQTLRKTLDG
                     P"
     gene            complement(5186..6082)
                     /gene="bezF"
     CDS             complement(5186..6082)
                     /codon_start=1
                     /gene="bezF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ubiA"
                     /gene_kind="biosynthetic-additional"
                     /product="prenyltransferase"
                     /protein_id="BBC27535.1"
                     /sec_met_domain="ubiA (E-value: 2.7e-34, bitscore: 108.1,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLHKISTGVVTQSPDPVRAYLRLMRLDRPTGYVLYLLPGLWAGIL
                     ASGRRPDLLAVVVLAVAAVLVRGAACAVNDVVDKDVDARVARTVSRPVASGTISVGNAL
                     LFAAAQAVVALLVLAVANTRTALVAAASYPLIVAYPYMKRVFFWPSAFLALVMSVYVLI
                     GWTAVTGSVDYPPAVYGLYVCAAFWTLIHDAIYSHQDKEYDRNIGVRSSALLFGKATKV
                     WLAAFVVVSVGGVLWAGSRTSTGWAFHLMVVLAGVFLAFLVVRVDLDDPKSCWDTFVAN
                     TYFGWLVLAAVVAGQLT"
     gene            complement(6093..7343)
                     /gene="bezE"
     CDS             complement(6093..7343)
                     /codon_start=1
                     /gene="bezE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 389.7; E-value:
                     2.4e-118)"
                     /gene_kind="biosynthetic-additional"
                     /product="cytochrome P450 monooxygenase"
                     /protein_id="BBC27536.1"
                     /sec_met_domain="p450 (E-value: 3.2e-22, bitscore: 68.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDSTSASTSTSTAWSEPRFRRRPSVSSPPAQLPFEQPNVLRLAP
                     GLRTLRSRGAVHRVRTVEGDEAWLVTGHAWVRRLLDDDRLGRSNPDPEAAAKDSGSALL
                     ASLLGGFATDHARLRALLEPHFSPERMLALRPYVEKLTRQLLDGLAARKPPVDLRQELA
                     LSLPIQVMCEWLGVPQEDKDRFGGWTQDAANVGDPARSKQGLGQLFGYCRQLVADKRRN
                     PGDDAISRICATEGIDDNEVLGLTALLLFGGYETTVARIGTCVSLLLADPEQWQALLRD
                     PDLVPGAVEETLRMSMPNPHTGGMPRYALTDFEIDGVTVRTGDFVLLNIIAANHDETAF
                     ADPDRVDVTRDTTGSLAFGHGAHHCVGAMLARMQLQVVLSQLVARFPTLRLAVGAEELR
                     LRDSTLIGGLVHLPVTW"
     gene            complement(7401..8525)
                     /gene="bezD"
     CDS             complement(7401..8525)
                     /codon_start=1
                     /gene="bezD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182:Polyprenyl synthetase (Score: 225.9; E-value:
                     9.7e-69)"
                     /gene_kind="biosynthetic-additional"
                     /product="polyprenyl synthetase"
                     /protein_id="BBC27537.1"
                     /transl_table=11
                     /translation="MGKARDRRRIPMSALDTSSAGPQALLSPRMVQSTVLPVIRQQVSA
                     LEPTLARICSYHLCFTDEHGAPSSVQGGKLTRAALMTAAADGVGLRPEDVRCQAAALEL
                     LHNSTLLHDDIIDADELRRGRPAAWTVFGAGPAILAGDALQALGLRMVVDDPGPGRADI
                     SAAFAEALALVLAGQARELTVRLGSADGTAEHDGTAEHDRVAEYDRIVEEKTSALLQCA
                     LVTPALRAGGPGHVLTALRGAGRHLGLAFQILNDVEDIWGDPRVTGKPVRSDILRRNPS
                     FPVLAALSTDTPAGDRLRRLWHADVTTGPHLQEMADLVEESGGRHTAEQRSRSHLHTAL
                     EHLGRAGLSPAASTELAALFDRIVHRGAGSRPTT"
     gene            8850..10193
                     /gene="bezC"
     CDS             8850..10193
                     /codon_start=1
                     /gene="bezC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 299.1; E-value: 7.9e-91)"
                     /gene_kind="biosynthetic-additional"
                     /product="cytochrome P450 monooxygenase"
                     /protein_id="BBC27538.1"
                     /sec_met_domain="p450 (E-value: 3.8e-10, bitscore: 28.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVGSGQLPGHRSFDPYGAHRDDPYSFLADIGETEPVFYAPLLDA
                     WCVTRREDIVAVLRDDRNFSARDHNPRSAVAFPKDVSRMLRTWRGTDALGVGSLDPPEH
                     ARVREVLNAGFTPARVRAFEPTMRGIADDLVERAAVTEEFDFITAFAVPYALEVICRLL
                     GIPDEYLDRCRTWSEQRIELMMLQRSTDPDRLRECARGLMDFGAFARSLVQERLASPQD
                     DLISELLHDGKAGHTLTADEASVQIPTLIFAGHMTCAESLGTIVFQQLRSPGGWAEVVD
                     GTIAARDLVEEGLRFDSPLAGMYRTALDDVLVGDVRLAAGSRLLLLYGAAGRGGRSPVC
                     PAGFSPGKGSATHLAFGHGIHFCLGAGFARMELEVAIRAVADRLPELTLAPGPPPRHRP
                     VFPLRALTELRVRQGGRAAGQLSGRAAGRAAGRAAGDLDDTLTAAELT"
     gene            complement(10352..11182)
                     /gene="bezB"
     CDS             complement(10352..11182)
                     /NRPS_PKS="Domain: oMT (30-224). E-value: 5.9e-45. Score:
                     145.0. Matches aSDomain: nrpspksdomains_bezB_oMT.1"
                     /codon_start=1
                     /gene="bezB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 197.8; E-value:
                     3.6e-60)"
                     /gene_kind="biosynthetic-additional"
                     /product="methyltransferase"
                     /protein_id="BBC27539.1"
                     /transl_table=11
                     /translation="MTLDQSPAPSDVRNVYDGFGRIYRTVWGPNIHYGYWENDADDSSV
                     EEATHRLTDLMISGLAARADQRVLDIGCGIGNPALRLVRACDVDVVGISVSHDQVARAT
                     ERATEAGLADRATFQFADAMDMPFPDGSFDAAWALESMWHMPDRGHVLAETARVLRPGG
                     RLAIADVIQRGPVGPEAQVVLDHIRENYAVRSLGTVDEYRTTLAANGFVDVEIRDITDN
                     VIRTLGLMADAYETVRDKLSDLVGEEKADSLIDFVRRFGAIPESGYLFLTAVRA"
     aSDomain        complement(10511..11092)
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_bezB_oMT.1"
                     /domain_subtype="oMT"
                     /evalue="5.90E-45"
                     /label="bezB_oMT.1"
                     /locus_tag="bezB"
                     /protein_end="224"
                     /protein_start="30"
                     /score="145.0"
                     /tool="antismash"
                     /translation="IHYGYWENDADDSSVEEATHRLTDLMISGLAARADQRVLDIGCGI
                     GNPALRLVRACDVDVVGISVSHDQVARATERATEAGLADRATFQFADAMDMPFPDGSFD
                     AAWALESMWHMPDRGHVLAETARVLRPGGRLAIADVIQRGPVGPEAQVVLDHIRENYAV
                     RSLGTVDEYRTTLAANGFVDVEIRDITDNVI"
     gene            complement(11338..12060)
                     /gene="bezA"
     CDS             complement(11338..12060)
                     /NRPS_PKS="Domain: oMT (31-187). E-value: 4e-17. Score:
                     53.2. Matches aSDomain: nrpspksdomains_bezA_oMT.1"
                     /codon_start=1
                     /gene="bezA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 94.5; E-value:
                     1.2e-28)"
                     /gene_kind="biosynthetic-additional"
                     /product="methyltransferase"
                     /protein_id="BBC27540.1"
                     /transl_table=11
                     /translation="MTPDAFRRSIRLHQEGLLRYAAKIWGAEHRLSGRILDVGCGLGGG
                     SLFWAQEYGADVTAVTNAPEHAPIVEGFARECGVGGRVRTLVCDAMHLPLDGGPYDAAV
                     AIESSGYFDRPVWFERLAHVLRPGGSVCIEEVFTTRPHGADVWAEYFYTKPATVLDYAE
                     AAKAAGFELVDDVDATSETLPFWEESTAWTKAVLDSDSTLSAVDRRQLRISLMANQALG
                     AEWQAGGLRLGFLRFERK"
     aSDomain        complement(11500..11967)
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_bezA_oMT.1"
                     /domain_subtype="oMT"
                     /evalue="4.00E-17"
                     /label="bezA_oMT.1"
                     /locus_tag="bezA"
                     /protein_end="187"
                     /protein_start="31"
                     /score="53.2"
                     /tool="antismash"
                     /translation="SGRILDVGCGLGGGSLFWAQEYGADVTAVTNAPEHAPIVEGFARE
                     CGVGGRVRTLVCDAMHLPLDGGPYDAAVAIESSGYFDRPVWFERLAHVLRPGGSVCIEE
                     VFTTRPHGADVWAEYFYTKPATVLDYAEAAKAAGFELVDDVDATSETLPFWE"
ORIGIN
        1 ccgccgagac cgccacggcc gtcatggctt cccccagcat cgacttcacc ggatagcggt
       61 acagcgccgc cacagtcccc atggatggat tatggggtgg attgaggatg gactgtggcg
      121 ctcaacaccg ccgcgcttca caggagttca gggtcgtggt gtctgcgggt acgtggtggc
      181 gccgccgggg tcgggggcgg catggtcttc gtccgccgct catggccggg tggggcgggg
      241 cggtctgtgg tggaccggct cgacgaggtc gtggtcgatg cccaggtcgt cgaggaggag
      301 tcgcaacggg ccggtgtccc gtgcgggcgc gggacgggcc gcggcccacg cgctcagctg
      361 accggtgtcg atctcgaagc cgccctgggc cgccacggtc acccaggggt ccaggtccgc
      421 ggagcggaac gcggtcagcc cggtgaccag ggcctcgcgg aacatccgct gccgtgtgcc
      481 gtccaggttc cggtagagcg agccgcacaa ctcgcggaac accaccgcgt gtccgccctc
      541 gtcccgccgg tgcagatcgg tggtgatccg gttcatcggc tggatctccc gggcggtggc
      601 gagagtggag aggaacccgc tgatcgtcgt ctcggcggcc agggagaacg cgatgtcgac
      661 gatgtcgcgg tcgaacccgg tcagtccggt gcggacctcg ttccgccccc gcacgaccga
      721 ccagacgtcc ggcgcgaacc gtaagttctc caggtcgcgc cgcctgcggg tgacgccgac
      781 ggcgttgtgg cacatcagga tgtggtagtg ctcgtcgatg atcgtctggt gcagcgcgtc
      841 gacggcgacg tcgtcgcggc ggacggggac gcgttcctgg agcatcaacc ggcaggcagg
      901 caggatgatc tcgtcctcga tggcggcggt cttggcgttg taggagatcc acgccgcgga
      961 caggatgcgc tcgcgcgcct cccggtcgag gcggtcggca cccggcaggt ccagcaccgg
     1021 gacgaggtgt tcggggaagt ccggcagcgc ctcgtcgaag tgaccgtcca ggtcgagctc
     1081 gtccttcttg acggccaccc gcttccccca gcggcgcgtc agctgcgcca gcaagtcgcg
     1141 ttcgtacgcg gcctcttcac gtgccacggt catcggggaa cctccttcgc gggtacgccg
     1201 ggggaagcgg cgcggcgtcg gccgggacgg cgagcgcggt caggcgggac tgccacagct
     1261 cggtcgccgc ggtgtccgcg ccccggaacc tgcccagtcc ctcggtgacc ggccggatgc
     1321 cgtgcagggc gttcagcgtc cagacgggca ggtcggcgag atcctgcggg gtggcccgtt
     1381 cggtgacgac ctggtgccct gccgcggcgg cgagttcgcc gagcagggcg cgggtgatgc
     1441 cggggagcag gccggggccg ggcggtggcc cgcagagcgt gtcgccccgc caccagacga
     1501 cgctcgtcgt cgccccttcg accacatgac cctccgccga gaccagcagc gcctcgtcgg
     1561 ctccggcccc ggtcgcggcg gcgcgcaggg aggccaggtg gtcgaggtcg acgcccttga
     1621 cggccggcag ccgtcggcgg tcgggtcccg gcgcggtcca caaccggacg gtcgcggtac
     1681 gcggtggtgc ggggcgcggc cacatccgca gccgcgttcc cgagccggtc tcgacgagtt
     1741 cgacgcgggg aaaccatcgg cctcgggcgg gcgatgcctc ggcgaccgcc cgcaggaacc
     1801 ggtcggtccg ttccgctccc ggtccacgga accggtcgca ggcggcggcg aaccgcgcgg
     1861 cgtgccggtc gaggccccgg acccgtccct cgtccaccag ccaggagtcg atgaccagca
     1921 ccgtcccggt ggagtcggcc gtcgaccatc cgccgtcggc atcccaccag cggcggacgc
     1981 cctggacggt ctcggcgtcc gtcgcagcgt cggtggctgc gtccctcgcc tccgccatcg
     2041 cgcccgtcgt cgcgtcggtc gtcccgtccc tcgcctccgc cgtcgtgccc gccgtcgtgc
     2101 ccatcgccgc gtcggtcgtc ccgtccgtcg tcccatccgt cgtcgtcgtg ttcatcgcgg
     2161 gccgccgccc acgtccgaca ccgtcggcgc acgccaggcg cggagtgacg ccgccgcctt
     2221 gagcagcatc tcctcgtact cctcctgcgg gtcggagtcg agcacgatcg ccccgccggc
     2281 tccgatggac agacggtcgc cgacccggac cgccgtgcgg atcacgatgt tgaggtccgt
     2341 gccgccggag aggccgaagt agccgagtgc gccggagtag acccctcggg cctccgtctc
     2401 caggcggtcg atgatctcca tggtgcgcag cttcggcgcc ccggtcatcg acccgcccgg
     2461 gaagcactgc cggacgcagt ccaccgcgcc gaccccgtcc ttgagccgtc cgcggacggt
     2521 ggagaccagt tggtgcacgg tctcgtagct ctcgacggcc agatacgggt cgacctcgat
     2581 gctgcccacc tcgcacaccc ggcccaggtc gttgcggagc aggtcgacga tcatcaggtt
     2641 ctcggcctgg gtcttggcgc tggaggcgag cgtggcggcc agttccgcgt cgcgcaccgg
     2701 gtcggcgtcg cgcggcgcgg tgcccttgat cggcttgctg gtgaccgtgc ggtcccgctc
     2761 gatgcgcagg aaccgctccg gcgaggagcc gaagaccgtg atgccgccga ggcgcagaaa
     2821 cgcggcgtac ggtgccgggt tggcccggcg cagccgcaga tagaaggacg tgtcgtcgtc
     2881 gtggaacggc aggtgcaaca tgtcggtgag gcagatctcg tagctctcgc cctggttcag
     2941 ctggcgtcgg cactccgcga tgtcggccag gtactggtcc cggtcgcggc cgaggtggtc
     3001 cccggcgtcg gagagcgggc ccgcggcggg tgcgtacgac ggtccggagt cggccggtgg
     3061 caggtccgtc aggagcgcgg aggtgcggtc cacccactcc tgcgcggcgc gccgggtccg
     3121 ctcgtcgtgg acggccacca ggtaggtcag cccctcctgg tggtcgacgg cgacgacacg
     3181 gtcggcgaac atccagacgg cgtccggtgt ttcggcggtg tggcgggcgg tggccccgca
     3241 ttcggccttg agttcgtagc cgaggtagcc gacatagccc ccggtcaggt cgaacggcag
     3301 gtccgggtcg gggacgcgcc gttcgcgcag ccgccgctcc aggacgtcga acacgctgcc
     3361 ggtgacgagg tggacgccgt gcgcgtcgcg tacttccact gccccgctgc cggtgcggta
     3421 ggtcaggacc tcgctcagcg gacccgaggt gtcgccgagg aacgagaacc gggacaggcc
     3481 ctcctccacc cggctgctgt ccagccagaa gcagtacggg aggttgtcga agaggcggag
     3541 aaagacggcc tcggaatcgg cggcggtcgg tacgaccgcg ctgatgatgc ccagtccccc
     3601 ggccgcgccg ggcaactcct gggccgcgcc gggcatctgc tcggcctcgg caagcgtctc
     3661 cgcgacagtg ccgggcatct cctccgcgag ggcggtcccg gtgtcggcgg aggcccgggt
     3721 gggggtgccg gtgaccgtga cggacggccg ctgtccgtgc cgcgtcccgc gggtcaggtc
     3781 ggcgaagttc cgcaggatct cgggcccgta ctgggaggcg atcgactcgg ggtggaactg
     3841 cacgccccac tgcggcagtt cgcggtggcg cagcgccatg accacaccgt cgtccgccca
     3901 cgcggtggcg acgagttcct cgggcagggg ctccggcacg cacagcgagt ggtagcgcac
     3961 cgcgacgaac tcacggggga ctccggcgaa cagcggatct ccgtcgtggg agaccttggc
     4021 gaggtgcccg tgtctcggtt cgggcgcggc gaccaccggc gctccggcca ggtgcgcgat
     4081 catctgatgg ccgaagcaga cgcccagcac gggcagcgtg gcgcggcgca gcagatcgcc
     4141 gaggaggccg acgtcgcggg ggttctgcgg ccgtccgggg ccgggtgaga ccacgatgtt
     4201 gtcgaagtcc gccagcggca gtccggccag cgacggatcg tcgttgacca tgacgaccgg
     4261 ctcccggccg ttgatgccgg cgatcaactg gtacaggttg aaggtatacg agtcgtagtt
     4321 gtccagcagc agggtacgca tcagggaccg tccaaggtct tgcggagcgt ctggacgtcg
     4381 cccggttcca gggtgatccc gaacatctcc tcgggagcgg agagcagctc gcccaggtcg
     4441 agttcccggg aggtcaccgg ctccgcggga cgtatttcca tgagggtgct gccggcgagg
     4501 aggtgccgca catcgggctg tgtgcgctgg aggaccatcc ggtggttgaa cggtgagcgc
     4561 ggatgggtga ggcagaagtg gttgaggagt tcgaagtccg acggatagcg cgggtccgtg
     4621 gtgaagccgt agagctccac cggtccctcc ggccgcagga accgcagcac gtgttcgccg
     4681 gtcggctcgc gcacgaggtc gtacggccag tcgccgtcca gccgggcgcc gtccgcgagc
     4741 cggatcggtt cgtgcagccc gtgggcgccg aaactcacgt cgtggagcca gcgttcgccg
     4801 tcgatctcca cgcacagggc cacatgcagg gccggcccga acctgctgcc ggtgcggatc
     4861 cgcgtccggc cggcgaacgc cgtgaacgtg aacccgatgc ggtccagcac ggccgccgtc
     4921 agcacgacct gttccagaca ccagccgccg cggccctgcc gcacgatctt ctcctggacg
     4981 ctctccaggt ccagcggtac cgctgtacgc ccgagcagcg cgtcgaggtt gtcgaaggct
     5041 atcgcgtcga catgggcgag gtgcaggccc cgcagcgtgg cgaggtccgg ggccacgtcg
     5101 ccctggtaac ccacccgggt cagataggcg tccaggtcga cgccgcttcc ctgccacatc
     5161 gaatccgtca cccgcggttc gtcgttcatg tcaactgccc ggcgacgacg gccgcgagga
     5221 cgagccagcc gaagtaggtg ttggcgacga aggtgtccca gcacgacttc ggatcgtcca
     5281 ggtccacccg tacgaccagg aaggccagga agactcccgc gagcacgacc atgaggtgga
     5341 acgcccatcc cgtcgacgtc cgtgaaccgg cccacagcac accgccgacg ctgacgacga
     5401 cgaaggccgc cagccacacc ttggtggcct tgccgaacag cagggcggag gacctgacgc
     5461 cgatgttccg gtcgtactcc ttgtcctgat gggagtagat cgcgtcgtgg atcagggtcc
     5521 agaaggccgc gcagacgtac aggccgtaca ccgcgggcgg gtagtcgacg ctcccggtca
     5581 ccgccgtcca gccgatcagc acatagacgc tcatcaccag ggccaggaac gccgagggcc
     5641 agaagaagac gcgcttcatg taggggtagg cgacgatcaa cgggtacgag gccgcggcga
     5701 ccagggcggt ccgcgtgttc gccaccgcca ggaccagcag ggcgacgacg gcctgtgcgg
     5761 cggcgaacag gagtgcgttg ccgacgctga tggttcccga cgcgacggga cgggacacgg
     5821 tgcgggcgac tcgggcgtcg acgtccttgt cgaccacgtc gttgaccgcg caggccgcgc
     5881 cgcgcaccag caccgcggcg acggcgagga cgacgaccgc gagcaggtcc ggtcgtcgtc
     5941 cggaggcgag gatgccggcc cagagccccg gaagcaggta gagcacgtac cccgtgggcc
     6001 tgtcgagtcg catcagccgg aggtaggcgc ggaccgggtc cgggctctgg gtgaccacgc
     6061 cggtggatat cttgtgcagc atcaggatcc catcaccacg tcaccggaag gtggaccagg
     6121 ccgccgatca acgtgctgtc gcgcagcctc agttcctccg cgccgacagc cagacgcagg
     6181 gtcgggaatc gtgcgacgag ctggctgagc accacctgca actgcatccg ggcgagcatc
     6241 gcgccgacgc agtggtgcgc gccgtggccg aaggcgaggc tgcccgtggt gtcgcgtgtg
     6301 acatcgaccc ggtcggggtc ggcgaaggcg gtctcgtcgt ggttggccgc gatgatgttc
     6361 agcaggacga agtcgccggt ccggacggtg actccgtcga tctcgaagtc cgtcagcgcg
     6421 taccggggca tgccgccggt gtgcgggttc ggcatcgaca tccgcagggt ttcctcgacc
     6481 gcgccgggga cgaggtccgg gtcgcgcagc agcgcctgcc actgctcggg gtcggccagc
     6541 agcagcgaca cacaggtgcc gatccgggcg accgtggtct cgtagccgcc gaacagcagc
     6601 agtgcggtga gtccgaggac ctcgttgtcg tcgatcccct cggtcgcgca gatcctggag
     6661 atcgcgtcgt cgcccgggtt gcgtcgcttg tcggcgacga gttgcctgca gtagccgaac
     6721 agttggccga gcccctgttt ggatctggcc gggtccccca cgttggcggc gtcctgggtc
     6781 cagccgccga accggtcctt gtcctcctgc ggcacgccca gccactcgca catcacctgg
     6841 atcggcagcg acagcgccaa ctcctggcgc aggtccaccg gtggcttccg cgcggccagt
     6901 ccgtccagga gctgccgggt cagcttttcg acgtagggcc gcagtgccag catccgctcg
     6961 ggtgagaagt gcggctccag cagcgcgcgc aaccgggcgt ggtcggtggc gaagccgccc
     7021 agcaggctcg cgagcagcgc ggacccgctg tccttcgccg cggcctccgg atcggggttc
     7081 gagcggccca gccggtcgtc gtcgagcaac cgcctcaccc aggcgtgacc ggtgaccagc
     7141 catgcctcgt cgccctcgac ggtacgaacc cggtggaccg cgccccggga ccggagcgtg
     7201 cgcagccctg gcgcgaggcg cagcacgttc ggctgctcga agggcaattg agctggggga
     7261 ctactcactg acggcctccg ccggaatcga ggttcggacc acgctgtcga agtcgacgtc
     7321 gaagccgatg tcgagtcgcc cagcggccgg ttgatcgggt cgcgggtcgc aggtcctggg
     7381 tcctgagtcc tgggtcctgg tcacgtggtg ggcctgctcc cggcgccgcg atggacgatg
     7441 cggtcgaaca gcgcggccag ttcggtggac gcggccgggg acagcccggc tcgtccgagg
     7501 tgctccagag ccgtgtgcag atggctgcgg gaccgctgct cggccgtgtg ccggccgccg
     7561 gattcctcta ccaggtcggc catctcctgg aggtgcggcc cggtggtgac gtcggcgtgc
     7621 cacagccggc gcagccggtc accggccggg gtgtcggtgg acagggccgc cagcaccggg
     7681 aaggacgggt tgcgtcgcag gatgtcgctc cgcacgggtt tgccggtgac ccgtgggtcg
     7741 ccccagatgt cctcgacgtc gttgaggatc tggaaggcga ggcccagatg ccggcccgcg
     7801 ccgcgcaggg cggtcaggac atgccccggt ccgcccgccc gcagtgcggg cgtcaccagg
     7861 gcgcactgca gcagcgcgga cgtcttctcc tccacgatcc ggtcatactc cgcgacccgg
     7921 tcgtgctccg cggtgccgtc gtgctccgcg gtgccgtcgg ccgaacccag tcggacggtg
     7981 agttcgcgag cctggccggc gaggaccagg gcgagggctt cggcgaacgc ggcggagatg
     8041 tcggcccgtc cgggtccggg gtcgtcgacg accatccgca gtccgagagc ctgcaacgcg
     8101 tcccctgcca ggatggccgg accggcgccg aacaccgtcc acgcggccgg gcgtccgcga
     8161 cgcagttcgt ccgcgtcgat gatgtcgtcg tggagcaggg tcgagttgtg cagcagttcc
     8221 agtgccgccg cctggcaccg cacgtcctcc ggccgaaggc ctacgccgtc ggccgccgcc
     8281 gtcatcaggg cggcgcgggt cagcttgccg ccctgcacgc tgctgggggc gccgtgttcg
     8341 tcggtgaagc agaggtggta ggagcagatc cgggccaggg tcggctccag tgcggaaacc
     8401 tgctgccgta tgacgggcaa taccgtggac tgcaccatcc tcggcgacag gagggcctgg
     8461 ggcccggccg acgaggtgtc gagtgcgctc atcgggattc tcctccggtc gcgggccttt
     8521 cccattcctg gtcagggcct gcgcgcatgg ggttcgcgaa ttgcaccact gaaatcactg
     8581 tgctggatat attgcgcaat tatcggggcc tgcgcagagc ccatgaatgc tgagccggaa
     8641 ttttcgtggc tgttccgaca gccatcctga acgacctctt cgtgttgtgt caacgacgcg
     8701 gcgtgagcgg cttcgacttt aggaaaaacc ccaaagaaca cggtacgggg tctggattag
     8761 actgcgttgc ggaatgatgg ctgccgggcg gcgcccgggc cgtattcgag gcttgccggt
     8821 tcactgaaag ctcgagaggc ggaacggcta tgtcagttgg ttctgggcaa ttacccgggc
     8881 acaggtcgtt cgatccgtac ggcgcacatc gcgacgaccc gtattcattt ctggccgaca
     8941 tcggcgagac ggagccggtg ttctacgccc ctttgctcga cgcgtggtgc gtcacccggc
     9001 gcgaggacat cgtcgccgtg ctgcgggacg accggaactt ctcggcgcgt gaccacaacc
     9061 cccgctccgc ggtcgcgttc cccaaggacg tgagccggat gctccgcacc tggcgcggta
     9121 ccgacgctct gggcgtcggc tcgctcgacc ctcccgagca cgccagggtc agggaggtgc
     9181 tcaacgccgg gttcacaccg gcccgggtca gggcgttcga acccacgatg cgtgggatcg
     9241 ccgacgacct ggtcgaacgg gccgcggtca cggaggagtt cgacttcatc acggcgttcg
     9301 ccgtgccgta cgcccttgag gtgatctgcc ggctcctcgg gattccggac gagtacctcg
     9361 accgctgccg cacctggtcc gagcagcgga tcgagctgat gatgttgcaa cgctccaccg
     9421 atcccgaccg gctgcgtgaa tgcgcccgcg ggctgatgga cttcggggcg ttcgcccggt
     9481 cgctcgtcca ggagcggctc gcctcgccgc aggacgatct gatcagtgaa ctgctccacg
     9541 acggcaaggc gggccacacc ctcaccgccg acgaggcttc tgtccagatc cccacgctga
     9601 tcttcgcggg ccatatgacc tgtgccgagt cgctgggcac gatcgtcttc cagcagctcc
     9661 ggtcgcccgg cggctgggcc gaggtggtcg acgggacgat cgccgcgcgg gacctggtcg
     9721 aggaggggtt gcggttcgac agtccgctgg cgggcatgta ccggaccgcg ctcgatgacg
     9781 tgctggtcgg cgatgtccgg ctggccgcgg ggagccgcct gcttctgctc tacggagcgg
     9841 ccggccgggg cggtcgctcc cccgtgtgtc cggcggggtt cagtccgggc aaggggtccg
     9901 ccacgcatct cgcgttcggg cacggcatcc atttctgtct cggggccgga ttcgcccgca
     9961 tggaactgga ggtcgcgatc cgcgccgtcg ccgacaggct gccggagctg actctggcac
    10021 cggggccgcc gccgcggcac cggccggtgt ttccgctgcg ggcactgacc gagctgcggg
    10081 tgcggcaggg cggacgggcg gcggggcagc tgtcgggacg ggcggcggga cgggcggcgg
    10141 gacgggcggc gggcgatctg gacgacacgt tgaccgccgc ggaactcacc tgactcgcct
    10201 ggtggccggg cccgccggtt cgaccggcgc tccgtgacgg cccgccggtc gaaccggcgg
    10261 gccgtctctg cttgtcgctg attgctggtc gctggtcgct ggtcggacgg ggtgccggac
    10321 cgtcaccgga gaccggtgac ggtgcccgtg gtcacgcccg gacggcggtc aggaagagat
    10381 agccgctctc cgggatggcc ccgaacctgc gcacgaagtc gatgagcgag tcggccttct
    10441 cctcgccgac cagatcggac agcttgtcgc ggacggtctc ataggcgtcg gccatgagcc
    10501 cgagggtgcg gatgacgttg tcggtgatgt cacggatctc cacgtcgacg aagccgttgg
    10561 cggccagcgt cgtgcggtat tcgtccaccg tgcccaggga gcgcaccgcg tagttctcgc
    10621 ggatgtggtc caggaccacc tgggcctcgg ggccgaccgg gccgcgctgg atgacgtcgg
    10681 cgatggcgag acggccgccg gggcgcagga cgcgggcggt ctcggcgagc acatggcctc
    10741 ggtcgggcat gtgccacatc gactccagcg cccaggccgc gtcgaacgaa ccgtcgggga
    10801 agggcatgtc catcgcgtcc gcgaactgga acgtggcccg gtcggcgagc ccggcctcgg
    10861 tcgcgcgttc cgtggcccgc gccacctggt cgtggctgac ggagatgccc accacgtcca
    10921 cgtcacaggc gcggacgagc cgcagcgcgg gattgccgat accgcagccg atgtccagga
    10981 cgcgctggtc ggcccgtgcg gcgaggcccg agatcatcag gtcggtcagg cggtgtgtgg
    11041 cctcctcgac ggagctgtcg tcggcgtcgt tctcccagta gccgtagtgg atgttgggac
    11101 cccacacggt gcggtagatc cggccgaacc cgtcgtacac attgcggaca tcgctgggag
    11161 cgggagactg atcgagcgtc atgacgaact ccgtagggtt gtgaaggacg cgggaaagcc
    11221 ggcggcgatc gtcagccgcc ccggaatccg gcggtgtcat cgaaaggcgg gcggaaaccc
    11281 gctgcgcccg gctgggcgaa cccagccgag cgcctgcctc agctaccgac tcttctctca
    11341 cttccgctcg aagcgcagaa aacccagccg caggcctccc gcctgccatt cggcgcccag
    11401 cgcctggttc gccatcaggg atatcctcaa ctgccgccgg tccaccgccg aaagagtgct
    11461 gtcgctgtcc agcaccgcct tcgtccaggc cgtgctctcc tcccagaacg gcagggtttc
    11521 cgacgtggcg tcgacgtcgt cgaccagttc gaagcccgcg gccttcgctg cctccgcgta
    11581 gtcgagcacc gtggcgggtt tcgtgtagaa gtactcggcc cacacatcgg ccccgtgcgg
    11641 gcgtgtggta aacacttcct cgatgcacac gctcccgccc ggacgcagca catgcgccag
    11701 ccgctcgaac cagacgggac ggtcgaaata cccgctgctc tcgatcgcca ccgccgcgtc
    11761 gtaggggccg ccgtccagcg ggaggtgcat cgcatcacag accagcgtcc ggacccttcc
    11821 gcccactccg cattcccgtg cgaacccctc gactatcggc gcgtgttccg gcgcgttggt
    11881 gaccgccgtg acatcggcgc cgtattcctg cgcccagaac agcgagcccc cgccgagacc
    11941 gcagccgacg tccaggattc tccccgacag ccggtgttcg gcgccccaga tcttggccgc
    12001 ataccggagt aatccctcct gatgcagtcg tatgctgcgg cgaaaggcgt ccggtgtcac
    12061 atcgcgagtg tgccggggcg cttcggagga agggtaataa ccgacgtggt agtgaatcct
    12121 gggtccagga ccgtatttgc gaacaagctt ggccgttttg tcgtcgtagt attggccgac
    12181 tattcttacc tcgtcctgcg gctccagcac tgtttgacgt gaacttgcaa gttcatccag
    12241 attcgacacg gtcgctcctc cagagctcgg atgccgacga tgttggcgaa tggcccgatc
    12301 cggtcggccg ccgcagcagc gcctcgtcgg aaatgcgcag agagccgccc tgatattgag
    12361 tggcggctat tggattccat cctggagggc ggccctggat cgtgtcaacg acgcgacgat
    12421 gcacagcccc aactttggga aaaaccccaa agaacacggc gcagggtctg gattggacgg
    12481 tcctgcggaa tgatagctgc tgggcgtcac ccgggctgta ttcgagtccg gcctattagt
    12541 ccggcgagcc cgtcaccgac gacatccgcg accgcgtgcg cggtccgtgg aacgggtggc
    12601 gaccgaggcg gggccgtcgg cccgaaccga tcgagagagc ggccctttcg ccgcccacca
    12661 cacccggtga ggagcactgt tcgagatggc gctgctggaa tccgtgcgcg acccggcgcg
    12721 gctgcgtgag ctgaccgccg gcgaactgcg cgacctcgcc ggggagatcc gcgcgttcct
    12781 gatcgaccgg gtctccgcga ccggaggaca cctcgggccc a
//
