BGC0001537 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001537 (Streptomyces sp. NBRC 110030)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	86,591	High	butyrolactol A	PKS

BGC0001537 - Region 1 - T1PKS

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Location: 1 - 86,591 nt. (total: 86,591 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 86,592
Label		BGC0001537

Tailoring functions

Oxidoreductases: dehydrogenase

APK71_RS29955: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

APK71_RS29960: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

APK71_RS29970: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: transferase

APK71_RS29925: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Hydrolases: esterase phosphatase

APK71_RS29975: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

APK71_RS29980: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: synthase

APK71_RS29920: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

APK71_RS29935: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

APK71_RS29940: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

APK71_RS29945: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

APK71_RS29950: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001537.5	1.00	PKS	butyrolactol A	Streptomyces sp. NBRC 110030
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001537.5	1.00	PKS	butyrolactol A	Streptomyces sp. NBRC 110030
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001284.4	0.66	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.66	PKS	YWA1	Aspergillus oryzae RIB40

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 86591:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-redmal - ohmal - ccmal) + (redmal) + (ccmal - ccmal) + (pk) + (ohmal - ohmal)
Non-colinear ordering:: APK71_RS29920 + APK71_RS29935 + APK71_RS29940 + APK71_RS29945 + APK71_RS29950

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

APK71_RS29920: mmal - mmal - mal - mal

PKS_AT (306..602): mmal

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 75.0%; Methylmalonyl-CoA: 70.8%; Methoxymalonyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 173.0
Ethylmalonyl-CoA: 171.6
Methoxymalonyl-CoA: 157.7
2-Methylbutyryl-CoA: 153.6
CHC-CoA: 127.5
Propionyl-CoA: 125.6
trans-1,2-CPDA: 123.3
Malonyl-CoA: 80.9
Isobutyryl-CoA: 71.1
Benzoyl-CoA: 57.8
3-Methylbutyryl-CoA: 35.1
inactive: 23.2
Acetyl-CoA: 21.6
fatty_acid: 20.7

PKS_AT (1303..1601): mmal

ATSignature: Malonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 184.1
Ethylmalonyl-CoA: 170.3
Methoxymalonyl-CoA: 148.1
2-Methylbutyryl-CoA: 113.2
Propionyl-CoA: 101.0
trans-1,2-CPDA: 98.7
CHC-CoA: 96.0
Malonyl-CoA: 91.8
Isobutyryl-CoA: 74.3
Benzoyl-CoA: 54.9
3-Methylbutyryl-CoA: 44.9
fatty_acid: 38.0
Acetyl-CoA: 29.8
inactive: 22.3

PKS_AT (3297..3564): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 178.3
inactive: 93.4
Methoxymalonyl-CoA: 63.4
Methylmalonyl-CoA: 46.5
Isobutyryl-CoA: 30.1
Acetyl-CoA: 19.8
Ethylmalonyl-CoA: 17.3
Benzoyl-CoA: 14.4
trans-1,2-CPDA: 12.6
CHC-CoA: 11.2
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -1.0
Propionyl-CoA: -1.7

PKS_AT (4801..5071): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 218.4
inactive: 110.3
Methoxymalonyl-CoA: 84.3
Methylmalonyl-CoA: 70.0
Propionyl-CoA: 45.3
Ethylmalonyl-CoA: 38.8
Isobutyryl-CoA: 38.4
CHC-CoA: 26.9
Acetyl-CoA: 25.4
Benzoyl-CoA: 20.9
2-Methylbutyryl-CoA: 18.8
fatty_acid: 14.6
trans-1,2-CPDA: 9.4
3-Methylbutyryl-CoA: 0.0

APK71_RS29935: mal

PKS_AT (547..829): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.8
inactive: 93.5
Methoxymalonyl-CoA: 62.6
Methylmalonyl-CoA: 51.3
Propionyl-CoA: 26.8
Isobutyryl-CoA: 24.6
Benzoyl-CoA: 24.5
Acetyl-CoA: 19.7
Ethylmalonyl-CoA: 19.4
fatty_acid: 17.6
CHC-CoA: 13.9
2-Methylbutyryl-CoA: 12.6
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

APK71_RS29940: mal - mal

PKS_AT (542..823): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.2
inactive: 86.9
Methoxymalonyl-CoA: 65.2
Methylmalonyl-CoA: 52.4
Propionyl-CoA: 31.5
Isobutyryl-CoA: 24.5
Ethylmalonyl-CoA: 22.1
fatty_acid: 20.3
Acetyl-CoA: 20.1
Benzoyl-CoA: 19.6
CHC-CoA: 16.0
2-Methylbutyryl-CoA: 15.7
trans-1,2-CPDA: 10.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (2149..2430): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 178.6
inactive: 93.0
Methoxymalonyl-CoA: 61.8
Methylmalonyl-CoA: 51.4
Propionyl-CoA: 27.6
Isobutyryl-CoA: 24.8
Ethylmalonyl-CoA: 21.2
Acetyl-CoA: 20.5
fatty_acid: 20.1
Benzoyl-CoA: 16.6
CHC-CoA: 16.1
2-Methylbutyryl-CoA: 14.0
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

APK71_RS29945: pk

PKS_AT (594..891): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Malonyl-CoA: 83.3%; Methoxymalonyl-CoA: 83.3%

minowa: Ethylmalonyl-CoA

Prediction, score:

Ethylmalonyl-CoA: 208.4
Methylmalonyl-CoA: 205.8
Methoxymalonyl-CoA: 167.3
2-Methylbutyryl-CoA: 144.6
trans-1,2-CPDA: 118.6
Propionyl-CoA: 109.9
CHC-CoA: 108.6
Malonyl-CoA: 103.2
Benzoyl-CoA: 70.7
Isobutyryl-CoA: 68.3
3-Methylbutyryl-CoA: 41.3
fatty_acid: 34.8
Acetyl-CoA: 31.3
inactive: 26.1

APK71_RS29950: mal - mal

PKS_AT (542..848): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 66.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 87.1
Methoxymalonyl-CoA: 52.2
inactive: 47.7
Methylmalonyl-CoA: 35.2
Propionyl-CoA: 28.2
Ethylmalonyl-CoA: 19.9
2-Methylbutyryl-CoA: 11.5
CHC-CoA: 11.3
fatty_acid: 10.3
trans-1,2-CPDA: 9.6
Isobutyryl-CoA: 8.4
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2072..2382): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; 2-Rhyd-Malonyl-CoA: 62.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 128.0
Methoxymalonyl-CoA: 79.4
inactive: 73.0
Methylmalonyl-CoA: 55.8
Isobutyryl-CoA: 39.0
Propionyl-CoA: 37.3
Ethylmalonyl-CoA: 32.6
fatty_acid: 28.2
trans-1,2-CPDA: 24.0
Benzoyl-CoA: 18.7
CHC-CoA: 15.5
3-Methylbutyryl-CoA: 10.6
2-Methylbutyryl-CoA: 9.7
Acetyl-CoA: 0.0

Link to PARAS substrate predictor