LOCUS       BGC0001550              5410 bp    DNA     linear   CON 07-DEC-2022
DEFINITION  Streptomyces katrae strain NRRL B-16271 contig33.1, whole genome
            shotgun sequence.
ACCESSION   BGC0001550
VERSION     BGC0001550
KEYWORDS    .
SOURCE      Streptomyces katrae
  ORGANISM  Streptomyces katrae
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            JNZY01000033.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:48:11
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5410
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5410
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[952:3560]"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      953..3560
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     cand_cluster    1..5410
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..5410
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001550"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5410
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            815..925
                     /locus_tag="IF09_RS45020"
     CDS             815..925
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lassopeptides)
                     predicted lassopeptide"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656044.1"
                     /locus_tag="IF09_RS45020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="keywimysin-related RiPP"
                     /protein_id="WP_106975170.1"
                     /transl_table=11
                     /translation="MKKAYEAPTLARLGSFRKKTGLLGRSGNDRLVLSKN"
     CDS_motif       815..877
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKKAYEAPTLARLGSFRKKTG"
                     /locus_tag="IF09_RS45020_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       878..925
                     /prepeptide="core"
                     /locus_tag="IF09_RS45020_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="-0.20"
                     /molecular_weight="1623.9"
                     /monoisotopic_mass="1622.9"
                     /core_sequence="LLGRSGNDRLVLSKN"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MKKAYEAPTLARLGSFRKKTG"
                     /leader_location="[814:877](+)"
     gene            953..2818
                     /locus_tag="IF09_RS0130345"
     CDS             953..2818
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 102.6; E-value: 3.5e-31)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057605464.1"
                     /locus_tag="IF09_RS0130345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_037636388.1"
                     /sec_met_domain="Asn_synthase (E-value: 2.9e-48, bitscore:
                     153.7, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTESHESAAPGPGDANFAVFPDREDAAAVARRFARPGLRTLSHPS
                     GRPWLVGHWHDDEIVTARAGSTALTLIGCCPLDASGLQRHAAKVQDVTQLDALARALPG
                     SFHLAAALDGRLRVQGTASGLRLVFHAHIDGVAVAATRADVLAAGLGREPDEERLAVRL
                     LWPVPHPLGEEPMWRGITAVPPQDALIVAADGRTVRHSRWWSPPEPVRTLADGAPQVRE
                     ALTEAVDARTRKGGVVSCDLSGGLDSTSICFLADRSPARVVASTWPGRDPADTDLQWAE
                     QAVGFLPDVEHVVWDADASPLVYDDLLSIDDLLDEPTIGVMDRSRVLHHLPGMAERGSR
                     LHLTGIGGDHVAWCSEAYYHRLLRTRPLTAVRQLRGFRALWQWPLSGTVRALADSRPYG
                     AWLADAAGRLRDPQPATVSTSLAWGMPPRLFDWVTPDAERAASRALREAARGVSPHHPD
                     RGLHSDLEQIRSGTRIIRQWDRMAARAGVPMASPFLDDRVIEACLAVRPHERVTPWAYK
                     PLLTAAMHGIVPEPCLRRTNKAAASMDAADGLRAHRADLMALWEDSRLERLGLVDGDVL
                     RRLAQRPSTPGLRDAILYSTIACEVWLRGLPPVKKSGAEPAAAPL"
     gene            2875..3129
                     /locus_tag="IF09_RS0130350"
     CDS             2875..3129
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602540.1"
                     /locus_tag="IF09_RS0130350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_030301136.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 4.8e-29,
                     bitscore: 88.8, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 6.4e-20, bitscore: 59.8,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLRFGADVSTADTDYGTVLLDERNGQYWELNPTGTLVVQTLMGG
                     GDEAAAADALVAEFDIDRSQAEQDIAALVTELRASGLAV"
     gene            3126..3560
                     /locus_tag="IF09_RS0130355"
     CDS             3126..3560
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602541.1"
                     /locus_tag="IF09_RS0130355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_030301138.1"
                     /sec_met_domain="PF13471 (E-value: 5.2e-28, bitscore: 85.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPSALERPPGVPFARRTAARLVLLPAIVLSLLPPRRIRAVLGV
                     LRRGARPAAAAEAKGARDAMCAVSLRCAGPRGCLPRSLGAALLCRLTGSWPTWCTGVRV
                     VPPFTAHAWIEAEGRPVDEGVPDDYFTRLMAVAPVSRPAR"
     gene            3614..5410
                     /locus_tag="IF09_RS0130360"
     CDS             3614..5410
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 328.1; E-value:
                     1.7e-99)"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602542.1"
                     /locus_tag="IF09_RS0130360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_078613549.1"
                     /transl_table=11
                     /translation="MYGLTAGHRAAAVTAAVLTLVGSALGLAQPLVAKNAVDASGHGRA
                     LGPLLALLAVLFVAEAATGAVGRFVLERMGEGVVRQLRHGLVARLLRLEMKEHGRHRGG
                     DLISRVTADTTLLREVVSQALVDLVTGALVAAGAVALMVWIDPLLLLLVALTVAAAAAV
                     VASLLKGIRAASERMQSSVGAIAADLERALGALPMVRVHRAEGREAARIGERVESAYSA
                     GVQTAKLASVMSPAVELAVQGSFLLVLVIGGLRVNGHDTSLGDLVAFLLYASYLVLPLS
                     SVFRAVGLMQRGMGAYQRIEHVLDLPVEPAEPAEQATPAVGARTLPQSPYTGQAGTPPA
                     LALRDVHFGYDPDRPVLHGVSFAVPHRRQVALVGRSGTGKSTIFALAARFYEPDSGTLL
                     FGGRPAVELSRSECRARIAVVDQSTHVVHGTLRDNITYAVPDADEAEVRRVVELARLDG
                     VVRRLPGGLSGVLGERGSTLSAGERQRVALARALLARPALLLLDEPTSHLDAINENALA
                     KVMRDVAGECALLVIAHRLSTVQHADRIIVLDNGRSVAAGCHGELLATSPSYRALATGQ
                     MLRPAGSPGAAPKAPPGTRPSP"
ORIGIN
        1 ggacatgcgc ggcttccttc gcgccctgcg aacggttggc ggtcggcggg tactcggcgg
       61 tgggcaggcg gacggcggcc aacggggtgc cggagtccag tgcgatgcgc actcggtgta
      121 cgacgccggc tgtctgtacg gccgtggctg acgccgcctc cgcatgggca gcctgaagcc
      181 ggtgactgga tggtgcctct cggatgtcca ctcgcatcac cagaggcgag cggtggtcag
      241 ctgtcgctgc ggtagaggtt tcgcggaccg acgtcacgcg cggcctcgaa tcgaccaacg
      301 agggcccttc ggggccgtcg aagaatctgc cctcgggagc aggcgtaatc tgcttcgcca
      361 ccggggcgcc cagccggtgc tccaggccgg tccacctcac ctcttcatct ggcgcgagcg
      421 agggaggaac ggccatggcc aacgctgaac tccccggccc cggctcccag tagccgacga
      481 ctcccggcta cgccgacgcg gaggggagtg cgtgggggag tgtcgcgcgc cccctgggtg
      541 atcgccgcgc gctggtggaa ccctcggctg agccgagggt ggctgagagg gatgcatggg
      601 gttggaaagg tttctcctgg ccagaaagtg cttgcgtcaa tgctgttcaa gctgcaaccg
      661 cgatcgtccg atgcgtcgga atggccatcc agcgcaaagc atttcgtacc gtatgattta
      721 tgcgcgttcg gttgctatgt gccggtgacg tgcagctagc ttgggccgta tgccgccgcg
      781 gatgcggtgg cggaggccga catgaggaga cgtcatgaag aaggcgtacg aagctccgac
      841 gctcgcgcga ctgggttcct tcaggaagaa gaccgggctg ctgggtcgct ccggcaatga
      901 ccgcctggtc ctcagcaaga actgacggtc gacggctcgt cgatccgaag ccatgactga
      961 atcgcacgaa agtgcggctc cgggccccgg cgacgcgaac ttcgcggtct ttcccgaccg
     1021 cgaggacgca gccgccgtgg cccggcgctt cgcccgcccc ggcctgcgca cgctgtccca
     1081 cccgtcgggt cggccctggc tggtcggcca ctggcacgac gacgaaatcg tcaccgcccg
     1141 cgccggctcc accgccctca ccctcatcgg ctgctgcccg ctcgacgcct ccgggctgca
     1201 gcgccacgcc gcaaaagtgc aggacgtcac gcagctcgac gcgctggccc gcgcgctgcc
     1261 cggcagcttc cacctggcgg ccgctctcga cgggcggctc agggtgcagg gcaccgcgtc
     1321 cggactgcgg ctggtgttcc acgcgcacat cgacggcgtc gcagtcgccg ccacccgcgc
     1381 cgacgtactc gccgccgggc tcggccgcga accggacgag gagcggctgg ccgtccgact
     1441 gctctggccg gtgccccacc ccctcggcga ggaaccgatg tggcgcggca tcaccgctgt
     1501 gcctccgcag gatgctctga tcgtggccgc cgacgggcgt acggtacgac actcgcgctg
     1561 gtggtcaccg ccggagcccg tacggacact cgcggacgga gcgccgcagg tccgcgaggc
     1621 cctcaccgag gccgtcgatg cccgcacccg caagggcggc gtggtcagct gcgacctgtc
     1681 cggcggactc gactccactt cgatctgttt cctcgccgac cgctcgccgg cccgtgtggt
     1741 ggccagtacc tggcccgggc gcgaccccgc cgacaccgat ctgcagtggg ccgagcaggc
     1801 cgtcggcttc ctcccggacg tcgagcacgt ggtgtgggat gccgatgcct ccccgctcgt
     1861 ctacgacgac ctgctgtcca tcgacgacct gctcgacgaa cccaccatcg gcgtcatgga
     1921 ccgctccagg gtcctgcacc acctgccggg catggcagag cggggcagcc gcctgcatct
     1981 gacgggcatc ggcggcgacc acgtggcctg gtgctccgag gcgtactacc accgcctgct
     2041 gcgcacccgc ccgctcaccg ccgtgcgcca gctgcgcggc ttccgagccc tctggcaatg
     2101 gccgctcagc ggcacggtcc gtgcgctggc cgactcgcgt ccctacgggg cgtggctggc
     2161 ggacgccgcc ggccggctgc gtgatccgca gcccgccacc gtcagcacca gcctcgcgtg
     2221 gggcatgccg ccgcgcctct tcgactgggt cacccccgac gcggagcgtg cggcgagtcg
     2281 ggcactgcgc gaggccgccc ggggcgtgtc cccgcaccac ccggaccgtg gcctgcactc
     2341 cgacctggaa cagatccgct ccggcacgcg catcatccgg cagtgggacc ggatggcggc
     2401 ccgtgccggc gtaccgatgg cctcgccctt cctcgacgac cgtgtcatcg aggcgtgcct
     2461 cgcggtccgc ccccacgaac gggtcacacc ctgggcgtac aagcccctcc tgaccgccgc
     2521 catgcacggc atcgtgcccg agccgtgtct gcggcggacc aacaaggctg cggcctccat
     2581 ggacgcggca gacggcctgc gcgcgcaccg ggccgatctc atggcgctct gggaggactc
     2641 ccggctcgaa cggctcggct tggtggacgg cgacgtgctg cgccgccttg cccaacggcc
     2701 gtccacgccc gggctgcgcg acgccatcct ttactccacg atcgcctgcg aggtatggct
     2761 gcgcggcctt ccgcccgtga agaagtccgg ggcggagccg gcggcggccc cgctgtagtc
     2821 cgcgcggtct tagaccaggc cctcgaccag gccctcgaca gaacggaaca caccatgccc
     2881 ctgcgtttcg gcgccgacgt ctccacggcc gacaccgact acggcacggt ccttctcgac
     2941 gaacgcaacg gccagtactg ggagctgaac cccaccggca ccctggtggt ccagaccctg
     3001 atggggggcg gcgacgaggc ggccgcagca gatgcgctcg tcgcggagtt cgacatcgac
     3061 cgctcgcagg cggagcagga catcgccgcc ctcgtcaccg aactgcgtgc ctcggggctg
     3121 gccgtatgac cacgccgagc gccctggagc ggccgcccgg agtgcccttc gcccggcgca
     3181 cggccgcgcg gctggtcctg ctgcccgcca tcgtcctgtc gctgctgccg ccgcgccgca
     3241 ttcgtgccgt gctgggcgtc ctccggcgcg gtgcccggcc cgccgccgcc gccgaggcca
     3301 agggggcccg ggacgcgatg tgcgcggtca gcctgcgctg tgccgggccg cgtggctgcc
     3361 tgccccggtc gctgggcgcc gcactgctgt gccggctgac gggcagctgg ccgacgtggt
     3421 gcaccggggt acgagtcgtg ccccccttca ccgcgcacgc ctggatcgag gccgagggcc
     3481 gccctgtcga cgagggggtg ccggacgact acttcacccg gctgatggcg gtcgccccgg
     3541 tgagtcgacc ggctcggtga ccgagcctgc gcaggggcca cgcgagccct cggtgtgggg
     3601 cgcggtcgcg accatgtacg gtctcaccgc cgggcaccgg gccgccgccg tcacggccgc
     3661 cgtgctcacg ctggtcggtt cggccctcgg actggcccag ccgctcgtcg ccaagaacgc
     3721 cgtggacgcg agcggacacg gacgggcact cgggccgctc ctcgcgctgc tggcggtgct
     3781 gttcgtcgcc gaagccgcga caggggcggt gggacgcttc gtactggagc gcatgggtga
     3841 aggcgtcgtg cgccagctgc gccacggcct ggtggcgcgg ctgttgcggc tggagatgaa
     3901 ggagcacgga cggcaccggg gcggcgatct gatctcccgc gtcaccgccg acaccaccct
     3961 gctcagggag gtggtgtccc aggcgctggt cgatctggtg acgggggctc tcgtcgcggc
     4021 gggagcggtc gcgctgatgg tgtggatcga cccgctgctg ctgctcttgg tcgccttgac
     4081 cgtggccgcc gccgccgcgg tcgtggcctc gctgctgaag ggcatccggg cggcttccga
     4141 acggatgcag agttccgtcg gggcgatcgc agccgatctg gaacgcgccc tcggcgcctt
     4201 gccgatggtc cgcgtgcacc gtgccgaggg ccgcgaggcg gcgcgtatcg gtgagcgcgt
     4261 cgaatcggcg tacagcgcgg gcgtgcagac cgcgaaactg gcgtcggtga tgagtccagc
     4321 ggtcgaactc gccgtccagg gatccttcct gctcgtgctg gtcatcggcg gtctgcgggt
     4381 gaacgggcac gacacctcgc tcggcgacct ggtcgccttc ctgctctacg cgtcctacct
     4441 ggtgctgccg ctgtcgtcgg tgttccgcgc cgtcgggctg atgcagcggg ggatgggtgc
     4501 ctaccagcgg atcgagcacg tgctggacct gcccgtggaa cccgcggaac ccgcggagca
     4561 ggccactccg gccgttggcg cccgcacgct cccgcagtcg ccgtacacgg ggcaggccgg
     4621 gacgccgccg gcgctggccc tgcgtgacgt ccacttcggg tacgacccgg accggccagt
     4681 gctgcacggc gtctcgttcg ccgtcccgca ccgcaggcag gtcgccctcg tcggccggtc
     4741 gggcacgggc aagagcacga tcttcgcgct ggccgccaga ttctacgaac cggactccgg
     4801 aaccctgctg ttcggcggac ggcccgcagt agagctgagc cgcagcgagt gccgggcccg
     4861 catcgccgtc gtcgaccaga gcacccatgt cgtccatggc accctccggg acaacatcac
     4921 gtacgccgta cccgacgccg acgaggccga agtccggcgc gtggtcgaac tcgccaggct
     4981 ggatggggtg gtcaggcggc tgcctggcgg cctgtctgga gtcctcggtg agcgcgggag
     5041 cacgctgtcg gccggagaac ggcaacgggt cgccctggcc agggcactcc tcgcccgacc
     5101 ggccctcctg ctgctcgacg agccgacgtc ccacctggac gcgatcaacg agaacgccct
     5161 cgccaaggtg atgcgggacg tcgccgggga atgcgccctt ctggtgatcg cgcaccggct
     5221 gtccacggtc cagcacgccg accggatcat cgtgctggac aacggccgca gcgtcgccgc
     5281 aggctgccac ggggaactgc ttgccaccag cccctcgtac cgggcgctgg ccacgggcca
     5341 gatgctccgc cccgcaggct ccccgggcgc tgcgccgaaa gccccgccgg gcacgcgccc
     5401 cagcccctga
//
