LOCUS       BGC0001554              5975 bp    DNA     circular CON 17-DEC-2020
DEFINITION  Methanosarcina barkeri str. Fusaro, complete sequence.
ACCESSION   BGC0001554
VERSION     BGC0001554
KEYWORDS    RefSeq.
SOURCE      Methanosarcina barkeri str. Fusaro
  ORGANISM  Methanosarcina barkeri str. Fusaro
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanosarcinales; Methanosarcinaceae; Methanosarcina.
REFERENCE   1  (bases 1 to 4837408)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C.,
            Glavina,T., Hammon,N., Israni,S., Pitluck,S., Goodwin,L.A.,
            Saunders,E.H., Schmutz,J., Larimer,F., Land,M., Anderson,I. and
            Richardson,P.
  TITLE     Complete sequence of chromosome 1 of Methanosarcina barkeri str.
            fusaro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4837408)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C.,
            Glavina,T., Hammon,N., Israni,S., Pitluck,S., Goodwin,L.A.,
            Saunders,E.H., Schmutz,J., Larimer,F., Land,M., Anderson,I. and
            Richardson,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2006) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B100, Walnut Creek, CA 94598-1698, USA
  REMARK    Protein update by submitter
REFERENCE   3  (bases 1 to 4837408)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C.,
            Glavina,T., Hammon,N., Israni,S., Pitluck,S., Goodwin,L.A.,
            Saunders,E.H., Schmutz,J., Larimer,F., Land,M., Anderson,I. and
            Richardson,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-AUG-2005) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2020 02:14:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,093
            CDSs (total)                      :: 4,020
            Genes (coding)                    :: 3,750
            CDSs (with protein)               :: 3,750
            Genes (RNA)                       :: 73
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 121 of 270
            Pseudo Genes (incomplete)         :: 175 of 270
            Pseudo Genes (internal stop)      :: 106 of 270
            Pseudo Genes (multiple problems)  :: 96 of 270
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-5707d64f
            Run date                          :: 2025-08-15 19:41:25
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5975
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP000099.1.
            URL -- http://www.jgi.doe.gov
            Source DNA and bacteria available from Kevin Sowers
            (sowers@umbi.umd.edu)
            Whole genome sequencing and draft assembly at JGI-PGF
            Finishing done by JGI-LANL
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Contacts: Paul Richardson (microbes@cuba.jgi-psf.org)
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..5975
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     subregion       1..5975
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001554"
                     /subregion_number="1"
                     /tool="antismash"
     gene            1..393
                     /db_xref="GeneID:3626588"
                     /gene="cfbA"
                     /locus_tag="MBAR_RS01875"
                     /old_locus_tag="Mbar_A0344"
     CDS             1..393
                     /EC_number="4.99.1.3"
                     /codon_start=1
                     /db_xref="GeneID:3626588"
                     /gene="cfbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023539.1"
                     /locus_tag="MBAR_RS01875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Mbar_A0344"
                     /product="sirohydrochlorin nickelochelatase"
                     /protein_id="WP_011305381.1"
                     /transl_table=11
                     /translation="MTEKLGILAIGHGSKLPYNKEVVTQIADYIARKHSDVVVRAGFME
                     NSEPTLGEAIEGFSGTGVTKIAAVPVFLASGVHITKDIPRILSLDENGCGTLEIDGKTV
                     PLCYANPLGADELIADLVFKRVQEAL"
     gene            420..2006
                     /db_xref="GeneID:3626589"
                     /gene="cfbE"
                     /locus_tag="MBAR_RS01880"
                     /old_locus_tag="Mbar_A0345"
     CDS             420..2006
                     /codon_start=1
                     /db_xref="GeneID:3626589"
                     /gene="cfbE"
                     /inference="COORDINATES: protein motif:HMM:NF033197.0"
                     /locus_tag="MBAR_RS01880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Mbar_A0345"
                     /product="coenzyme F430 synthase"
                     /protein_id="WP_011305382.1"
                     /transl_table=11
                     /translation="MDLYRKKFAVLDLTHGGIPIAKSLAAAGNEVSGIDVYRTVKPETL
                     LELEEKYGIHCSKTPLPASGFDLIVAPVHLDPAYPMLLEARAQKKKVLSHHEIVGEILK
                     GDSRLSSIKTVEVTGVKAKTSTASLLADMLSRQFEVVLHTSRGLECWKAGASSLIYKGL
                     SITPGSILVAVEKTFEAGFRPDFFIFEISIGGTGTADLGILTTLSPDYGIANNTALASE
                     AKLQLIKHAKSGSTLLINAGAEKALKAAKKDQVKVLTFRDPFNDQLSATSVQIADFVLE
                     TVPETSALSVPSELVMPSGLQKPAGLSGSSEPDSLSGSSTSAGLSDSSMPAGSTEFQAS
                     PTPSGSDIRFMRRGKKILSASLRPGYNVSAYRTAFVAASAAAIELGTKPEAIISAIEGF
                     RGLSGRMQEKEMNGVSLIDNSNSGMDILSAEKALDYALLKRKDEKKEGIILVLGEESSQ
                     VCEGLPPELVQGFIEKFGAKCKQVILVGERMKAVNGKNISYARDLTEGLSKASEFAGGD
                     DIILSSVKCFR"
     gene            2442..3554
                     /db_xref="GeneID:3626590"
                     /gene="cfbD"
                     /locus_tag="MBAR_RS01885"
                     /old_locus_tag="Mbar_A0346"
     CDS             2442..3554
                     /EC_number="6.3.3.7"
                     /codon_start=1
                     /db_xref="GeneID:3626590"
                     /gene="cfbD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011023536.1"
                     /locus_tag="MBAR_RS01885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Mbar_A0346"
                     /product="Ni-sirohydrochlorin a,c-diamide reductive cyclase
                     catalytic subunit"
                     /protein_id="WP_011305383.1"
                     /transl_table=11
                     /translation="MAEKEISIIHPRPSSIVAALYTLRDLNVDVAIMHGPPGCSFKHAR
                     LLEEDGIHVVTTGLDENNFVFGGHDKLVQLINKSVELFNPKLIGIVGTCPSMIIGEEMH
                     DAVLEANPDVPVIEVEVHAGYHDNTKGVLFALESALDAGIIDHKEFERQKYLLEKATEV
                     EKKFGAASREYLAPSRGDVKYKAAQRVIQLLKEGKKGLVIMNAKKETGYMFADITLAVN
                     EVAEALGNKENLINMANIDPELGLPRVRQHAEYITRDLKAHGVEVHEIIGGMDEYPIAG
                     EKVSELIKEKYSDFDFAVISGVPHAIPMENIKNMELISITNGPRQVLPLKEMGHEYVLV
                     EIDLHPKTLGVSGIVESEFGATLREVAKEA"
     gene            3592..4389
                     /db_xref="GeneID:3626591"
                     /gene="cfbC"
                     /locus_tag="MBAR_RS01890"
                     /old_locus_tag="Mbar_A0347"
     CDS             3592..4389
                     /codon_start=1
                     /db_xref="GeneID:3626591"
                     /gene="cfbC"
                     /gene_functions="other (smcogs) SMCOG1157: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411155.1"
                     /locus_tag="MBAR_RS01890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Mbar_A0347"
                     /product="Ni-sirohydrochlorin a,c-diamide reductive cyclase
                     ATP-dependent reductase subunit"
                     /protein_id="WP_011305384.1"
                     /transl_table=11
                     /translation="MKNQKIIAIYGKGGIGKSSTASNVAAACAEAGKKVMIIGCDPKSD
                     SSITLLRGRRIPTILDLLREGVDIKKEDVVFEGYAGVKCVEAGGPEPGIGCAGRGIIVA
                     IQKLKSISGNLLKEQDLIIYDVPGDIVCGGFVAPVRKGFVNEAYVLTSGEYMPLYAANN
                     ICKGLSKIGMPLSGVICNSRNVSREEEIVSKFSEEIGSQLMAFIPKRQVVQDCEREGYS
                     VMEKAPESDIAEIYRKLGKAILENEKRVTADSLSDERLRELTK"
     gene            4491..5975
                     /db_xref="GeneID:3626592"
                     /gene="cfbB"
                     /locus_tag="MBAR_RS01895"
                     /old_locus_tag="Mbar_A0348"
     CDS             4491..5975
                     /codon_start=1
                     /db_xref="GeneID:3626592"
                     /gene="cfbB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037854.1"
                     /locus_tag="MBAR_RS01895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Mbar_A0348"
                     /product="Ni-sirohydrochlorin a,c-diamide synthase"
                     /protein_id="WP_011305385.1"
                     /transl_table=11
                     /translation="MLNDKQSVENIPRILISADRSSSGKTTISMGLMAALVSRGYKVQP
                     FKVALDYIDPSYHTEITGRFCRNLDGYLMDENGILDVYTHACEAGEKADIAIIEGVRGL
                     YEGFESLSDLGSTAQIAKILNCPVIFVINARSITRSSAALINGYRNFDPDVEIAGVILN
                     NIGSRRHAKKAKEAIEYYTGVPVIGIVPRDPAMQISMRHLGLMPALEGRRRLGDGGFEA
                     RLRGIEEIINKGIDVDRFMEIAKSAKALKSPENSVFSSVSDPGAPRPKIGVALDEAFNF
                     YYRDNIDLLNLAGAEIVYFSPVKDASLPEVDGLYIGGGYPELFAAELEANESMRQDIKK
                     ASAAGMPIYAECGGLMYLTEKISTGVPGKGTYHDASMPESTYSMVGALPGHTIMGQTRV
                     VSYNIGTLNKDCLLGKKYNSFKGHEFHHSEIREIPEDAEFAITLSRGTGIKNGMDGLIS
                     GNTLGSYAHLHGVAYREFASSLVEAARNFRDSRVLP"
ORIGIN
        1 atgacagaaa aactcggaat tctggccata ggccatggta gtaaacttcc atacaacaag
       61 gaagtagtca ctcagatcgc agattatata gcacggaaac actcggatgt cgttgtcaga
      121 gccggcttta tggaaaacag tgagccaacc cttggagagg caatcgaagg gttttcaggt
      181 acaggcgtga caaaaattgc agcagtaccg gttttcctgg catctggggt tcatatcaca
      241 aaggatatcc cgaggatcct gagcctggac gaaaatggct gtggtacctt ggaaattgac
      301 ggaaaaaccg ttcctctgtg ctatgcaaac cctcttggag ccgatgagct tatcgccgac
      361 ctggtattca aaagggttca ggaagccctt taaattcata tttggggtcc ggttttatca
      421 tggacctgta ccggaagaag ttcgccgtgc tcgacctgac ccacggcggc attcctatcg
      481 caaaaagtct tgcagccgct ggaaatgaag taagcggaat agatgtatac aggacagtca
      541 aacctgaaac gctgcttgag cttgaagaga agtacgggat acactgctca aaaactcctc
      601 ttcctgcctc aggattcgac ttgattgttg cgcctgtaca ccttgatcct gcctatccga
      661 tgcttttaga agccagggca cagaaaaaaa aagtcctttc gcaccatgaa attgtcggag
      721 aaattctcaa aggcgattca aggctttcaa gcataaaaac agtcgaagtt accggcgtaa
      781 aagcaaaaac aagtacagca tccctcctgg ccgatatgct ctccaggcaa tttgaagtag
      841 tgttgcatac ttcaaggggg cttgaatgct ggaaagcggg tgcttcttct cttatataca
      901 aaggcctgag cataactcct ggaagcattc tggttgccgt tgagaaaact ttcgaagccg
      961 gattcaggcc ggattttttt atctttgaga tctcaatagg aggcacaggc actgctgacc
     1021 tggggattct tacaaccctt tccccggact atgggattgc aaacaataca gctcttgcaa
     1081 gcgaagcaaa actccaactt attaagcacg caaaatctgg aagtactctt ttaatcaatg
     1141 caggagccga aaaagccctt aaagctgcaa aaaaagatca ggtaaaggtt ttaactttca
     1201 gagacccgtt caatgatcag ctttccgcga cttcagtcca aatagccgac tttgtactgg
     1261 aaacagtacc tgagacttca gccctatcag taccctcaga gttagtaatg ccttcaggtt
     1321 tacaaaagcc agccggccta tcaggttcat cagaaccaga cagtttatca ggttcatcaa
     1381 cctcagccgg cctatcagat tcatcaatgc cagcaggttc aaccgaattc caggcttcgc
     1441 ctacaccttc aggttccgac attcgcttta tgcgcagggg aaagaaaata ttatcagctt
     1501 ccctgcgtcc tggatataat gtttcggctt acaggactgc ttttgtcgca gcgtcggcag
     1561 cagccatcga acttggaact aaacctgaag ctattatttc ggcaatcgaa ggattcagag
     1621 ggctttcagg caggatgcag gaaaaggaaa tgaacggcgt ctccttaata gacaattcca
     1681 attccggcat ggatatcctc tcggccgaaa aagcccttga ttacgctctc cttaagagaa
     1741 aggacgaaaa aaaggaaggt atcatccttg tgttgggaga agaatcctct caggtctgtg
     1801 aagggctacc tcctgaactt gtgcagggtt ttatagaaaa gttcggtgca aagtgtaagc
     1861 aggttatcct ggtaggagaa cggatgaagg cagtaaatgg aaaaaacatt tcttatgcac
     1921 gagatctcac agaagggctt tcaaaagcct cggaatttgc aggaggcgac gatataatac
     1981 tttcttcggt aaaatgcttc cgttaaaatt atgttataat gcttccgtta aaattatgtt
     2041 ataactgtat aatgaggctc ttcgctgagg aatattgagg aattgttgaa gaattcccag
     2101 aaattccggt taattcgggc attacgaaaa ttactcagga atttctgctc aggataacaa
     2161 tttctaatat acaaatattt aatatataat ttggatccga gtctattccg gatacaagcc
     2221 gtaataggct gcaagcattt gccgaaattt ttatgataga gttttataca ggatcttcca
     2281 atctgtattc gtttccatta ctcactttaa tttcaactga tcgtgtttcc caatcccatt
     2341 ttacaggtaa tagtttatat cctgaggaca caggagtttt cagggaaatg cgttctatca
     2401 ttcttatcag tatccatatt atctaaacaa gaggatttgt tatggctgaa aaagagattt
     2461 caatcattca tccccgccca agttccatag ttgcggctct ttacactctg agagacttaa
     2521 atgttgatgt tgctatcatg catgggcctc cagggtgttc ctttaaacat gcaaggctgc
     2581 tcgaagaaga cggtatacat gtggttacca caggccttga tgagaacaac ttcgttttcg
     2641 gagggcatga caaacttgtc caacttatca acaagtcagt ggaacttttt aaccctaaac
     2701 ttataggtat tgtaggaacc tgccccagca tgattatagg ggaagaaatg catgacgccg
     2761 tgctcgaggc aaatcctgat gtccctgtaa tcgaagtcga ggtgcacgca ggctatcacg
     2821 ataacacaaa aggagtactc tttgccctgg aatctgcact cgatgcaggg attattgatc
     2881 acaaagagtt cgagcgtcag aaatacctcc ttgaaaaagc tacagaagtg gaaaaaaagt
     2941 ttggggctgc aagcagggaa taccttgctc cctcccgcgg ggatgtcaaa tacaaagccg
     3001 cacagcgtgt aattcaactt ctcaaagaag gcaaaaaggg ccttgtcatc atgaatgcca
     3061 aaaaagaaac cggctacatg tttgcagaca ttacccttgc agtcaacgag gttgcagaag
     3121 ctctagggaa taaagaaaac cttatcaata tggcaaatat cgacccggag cttgggcttc
     3181 ccagagtaag gcagcatgcc gaatatataa caagagacct caaagcacat ggagttgagg
     3241 tgcatgaaat cattggcggc atggacgagt acccgattgc aggcgaaaag gtaagcgaat
     3301 taataaagga aaaatacagt gactttgatt ttgcagtcat ttcgggtgtt ccacatgcaa
     3361 tccctatgga gaacataaag aacatggaac tgatttcgat aactaacggc cctagacagg
     3421 tcctgccctt aaaagagatg gggcatgagt atgtgctggt agaaatcgac ctccacccga
     3481 aaacactggg agtcagtggg attgtggaat ccgaatttgg ggctacctta agggaagttg
     3541 caaaggaagc ctgagcaaga agagacagga taaaatcagg aggaagatcc tttgaaaaac
     3601 cagaagatca tagcgatcta cggaaagggc ggcataggta aatcgagtac agcttcaaac
     3661 gttgccgctg cctgcgccga ggcaggaaag aaggttatga ttataggctg cgatcctaaa
     3721 agtgactcat caatcaccct tctaagaggc aggagaattc caaccattct cgacctcctg
     3781 cgtgagggtg tggacataaa gaaagaagat gtggtctttg aaggatatgc aggcgtcaaa
     3841 tgcgtggaag caggcggccc agaaccaggc ataggttgtg cagggcgagg aatcattgtt
     3901 gccatccaga aattgaagag catttcagga aatctcttaa aagagcagga tttgatcatt
     3961 tacgatgtgc ccggagacat agtctgcgga gggtttgtcg cccctgtcag gaaaggattc
     4021 gtaaatgaag cttatgtcct gacctcagga gagtacatgc ccctttatgc agcaaataat
     4081 atatgcaaag gcttatcgaa gataggaatg ccgcttagcg gggtaatctg taattcaaga
     4141 aacgtgagca gggaagagga aatcgtttcg aagttttctg aggagatcgg cagccagctt
     4201 atggctttta tcccgaagag gcaggtagtt caggactgtg aaagagaagg ttactcggta
     4261 atggaaaaag cgcctgaatc cgatattgca gagatctacc gcaaactcgg aaaagcaatc
     4321 cttgaaaacg aaaaacgagt tacagccgat tcccttagtg atgaacggtt gagggaatta
     4381 acaaaataaa attagagtgt taatagttga ataaacacat tagggtgaat aaacacatca
     4441 gggtaaataa atacatcagg gtaaatgaac acaaaagggg tatcgaaaaa atgcttaacg
     4501 acaagcaatc cgtagagaat attcccagga ttctcatttc cgcagaccgt tcttcctcag
     4561 gcaagactac aatctccatg ggcctgatgg ctgctctcgt ttcaagggga tacaaagtcc
     4621 agccctttaa ggtcgctctg gactatatcg acccgagtta tcatactgaa ataactggca
     4681 ggttctgccg gaaccttgac ggctacctta tggatgagaa cgggattctg gatgtctata
     4741 ctcatgcctg cgaggccggc gagaaggcgg atattgcgat tattgaagga gttcgcgggc
     4801 tttatgaagg gtttgaaagc ttaagcgacc ttggaagcac tgcccagata gcaaagatcc
     4861 ttaactgtcc cgtaatcttc gtaatcaatg ccagaagcat tacccgttcg agtgctgccc
     4921 ttatcaacgg atataggaac tttgaccctg atgtggaaat tgcaggagtc atccttaata
     4981 acatcggaag ccgccgacat gccaaaaaag caaaagaggc aatcgagtac tatacaggcg
     5041 ttccggttat tggaattgtt ccaagagacc ccgcgatgca aatttccatg cgccatctag
     5101 ggcttatgcc agctctcgaa ggccggagac ggcttgggga cggaggcttt gaagcccgcc
     5161 ttcgcggtat tgaagaaatc atcaataaag gaattgatgt agaccgcttt atggaaatcg
     5221 caaaaagcgc aaaagccctg aaaagccctg aaaatagcgt tttttcttcg gtttctgatc
     5281 ccggagcacc caggccaaag ataggggtcg cccttgatga ggctttcaac ttttactatc
     5341 gcgataacat cgatcttcta aatcttgcag gcgcagaaat cgtttacttt agccctgtta
     5401 aagacgcctc acttccagag gttgatgggc tctacatcgg aggaggttac cccgaactct
     5461 ttgcagccga acttgaagct aacgagtcca tgcgccagga tatcaaaaag gcttctgctg
     5521 caggcatgcc tatttatgcc gagtgcggag ggcttatgta ccttacggaa aaaataagca
     5581 caggcgtccc cggaaaaggt acctatcacg atgcctcaat gccggaatct acatattcaa
     5641 tggttggagc cctaccaggg catactataa tgggacagac aagagtggtc agctacaata
     5701 tcgggaccct taataaggac tgccttctcg ggaaaaaata caacagtttc aagggacacg
     5761 aattccacca ttctgaaata agggaaattc ctgaggatgc tgaatttgca ataaccctgt
     5821 caagaggtac aggcataaag aacggtatgg acgggcttat ttctggaaat accctgggct
     5881 cgtatgccca cctgcacgga gttgcgtaca gagagtttgc aagctcactt gtcgaggctg
     5941 cacgaaattt tagggattcc agggttcttc cgtga
//
