LOCUS       BGC0001576             16772 bp    DNA     linear   BCT 08-DEC-2017
DEFINITION  Prochloron didemni divamide A gene cluster, complete sequence.
ACCESSION   BGC0001576
VERSION     BGC0001576
KEYWORDS    .
SOURCE      Prochloron didemni
  ORGANISM  Prochloron didemni
            Bacteria; Cyanobacteria; Synechococcales; Prochloraceae; Prochloron.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 02:39:25
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..16772
                     /db_xref="taxon:1216"
                     /host="Didemnum molle"
                     /mol_type="genomic DNA"
                     /organism="Prochloron didemni"
     misc_feature    1..16772
                     /note="divamide A gene cluster"
     subregion       1..16772
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001576"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..16772
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     protocluster    1458..16772
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[11457:14712]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      11458..14712
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1458..16772
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     CDS             complement(3741..3950)
                     /gene="allorf_03741_03950"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="allorf_03741_03950"
                     /protein_id="allorf_03741_03950"
                     /tool="antismash"
                     /transl_table=1
                     /translation="XTPDSKETSXXXILRHPPSKXIKVPKREKSRRKXQFWXFKSSAIS
                     RQIISACCXLTHFSKTIKSLPXPLP"
     CDS_motif       complement(3798..3950)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence:
                     XTPDSKETSXXXILRHPPSKXIKVPKREKSRRKXQFWXFKSSAISRQIISA"
                     /locus_tag="allorf_03741_03950_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(3741..3797)
                     /prepeptide="core"
                     /locus_tag="allorf_03741_03950_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-6.80"
                     /molecular_weight="1813.2"
                     /monoisotopic_mass="1812.0"
                     /core_sequence="CCXLTHFSKTIKSLPXPLP"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="XTPDSKETSXXXILRHPPSKXIKVPKREKSRRKXQFWXFKS
                     SAISRQIISA"
                     /leader_location="[3797:3950](-)"
                     /alternative_weights="1831.2"
                     /alternative_weights="1849.3"
     gene            4286..4636
                     /gene="divN"
     CDS             4286..4636
                     /codon_start=1
                     /gene="divN"
                     /product="DivN"
                     /protein_id="ARD09209.1"
                     /transl_table=11
                     /translation="MYKGLDRDEIRQIQVLMLLCLCLSPQSKLRQLLEIALAASETQIM
                     TRMTPCDDVNVDGLFTWVQSLFAQGGLTEEEKRLLKWQNESRNMLPAIDELKTIEKKLG
                     FKIRIQKLQSHN"
     gene            complement(4935..5792)
                     /gene="divorf1"
     CDS             complement(4935..5792)
                     /codon_start=1
                     /gene="divorf1"
                     /gene_functions="other (smcogs) SMCOG1226:hypothetical
                     protein (Score: 248.2; E-value: 2.1e-75)"
                     /gene_kind="other"
                     /note="putative formylglycine-generating enzyme"
                     /product="DivU"
                     /protein_id="ARD09208.1"
                     /transl_table=11
                     /translation="MMTTPPLQVFEFDVVTVDARGRKASRRRSQARYFTEDLGSGVTLE
                     AISIPGGEFVMGSTNKEAGAYERPHHKVTLEPFFMGKYQVTQAQWRAVAALPKSNRHLD
                     TDPSWFKDPPSSRGSRPVEQVSWYDAVEFCERLSRKTGREYRLPSEAEWEYACQAGTNA
                     PFHCGETITTDLANYNGDHYGSGPEGEYRQETTTVGSFPPNVFGLCDMHGNVWEWCADF
                     WHHEYTDSPTDGSVWLEGGDRLHRPGRGGSWLNDPRYCRSACRRPNEADSRYNCLGFRV
                     VCVF"
     gene            6263..8203
                     /gene="divT"
     CDS             6263..8203
                     /codon_start=1
                     /gene="divT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 120.6; E-value:
                     1.5e-36)"
                     /gene_kind="transport"
                     /note="ABC transporter"
                     /product="DivT"
                     /protein_id="ARD09207.1"
                     /transl_table=11
                     /translation="MSETHRFDRQFWQRFLALAQPYWYPISGSGVAFLGLLLLLMSLLL
                     TVSFIAVSMVSLLGQSYFPNVLNSIAPGLAESILKIIQSPAIYLVALCFPVTLVSFVYY
                     KRKLQEHWRPWLILTLLLLLLLGSIGMQVLNSYLGRDLITAMAQGKESSYFLLLLLYGA
                     SLIFVVPLVVLSHYVRKKLSIHWQQWLTNEFLAKYFYQKAYYDLKTDLKIDNPDQRLSK
                     EIEFFTQTIMDFVVTIFNQLINLIAFGIVLWSISKYLVAILIVYAVVGNLITVISSRRL
                     VDLNTDKLQYEANFRYSLIRVSDNAESIAFSRGEDRELSLVKQRFTQLVKNINRMIGWE
                     RNIELLSVGYQNILFIIPFLVVGPLYFSGQVELGVITQASMACAQLSAALSVIVSRFES
                     LSALAAVVNRLATFTEALTVSETTEKSVNTIEPVEDNRFAIEALTLQTPNREQTLLKEL
                     SVSVEPGENVLIVGASGCGKSSLLRAIAGLWNAGTGRILRPNLEEILFLPQRPYMILGS
                     LREQLLYPNGDRETPDDKLESVLHQVNLPQLLGKVGGFEAELDWSNVLSLGEQQRLAFA
                     RLLLNRPRYAFLDEATSALDLQNEKHLYQQLQYSNITFVSVGHRESLLNYHQRVLKLLG
                     DANWQLFSAML"
     gene            complement(8777..9574)
                     /gene="divMT"
     CDS             complement(8777..9574)
                     /codon_start=1
                     /gene="divMT"
                     /note="N-terminal methyltransferase"
                     /product="DivMT"
                     /protein_id="ARD09206.1"
                     /transl_table=11
                     /translation="MENNNYPFELKAYDFSFKIFKEIIFALNAFFIGFWLGVLKREHYH
                     LVDSIYYNQTEMYRDENYNKRGLWDWEEKVLAQYFQQCHNLLVVAAGGGREVLALCKRG
                     YEVDGFECNANLLKFANNLIKQEEFASHIKLAPRDQCPDSQKEYDGLIVGWGAYMLIQG
                     KERRIEFLRQLRTQAKKNSPVLLSFFCYSETTGGRDFKAIAMIGNAFRRLLGRECLEVG
                     DNLAPNYVHYFTKDEIASELQAGGFELKIYCTNQYGHAVGIAV"
     gene            complement(9680..10459)
                     /gene="divY"
     CDS             complement(9680..10459)
                     /codon_start=1
                     /gene="divY"
                     /product="DivY"
                     /protein_id="ARD09205.1"
                     /transl_table=11
                     /translation="MKIFLTCLLALALLLGMPSSAFAFKVPIHEEITREVFEDFQVVVE
                     GETFKFTDYAIDQIVKANKDTDDLPNQFNTEMHFDGEDFSGGSNRVMFLKERTITKVTD
                     PQDPQGTSARNDLGTALHTVQDFYAHSNWVELGHSSSDINTKIGREVFSGADKNTATCP
                     NDPGILGGAGLTELTSGYFTFIGVVPSCDVPEGKCRHGVPIVCPDGLNKDDNSRPGFPT
                     ARALAVKATEDFLNQIFSDSRMDGNVDAIKLLMRIRN"
     gene            complement(10470..11387)
                     /gene="divX"
     CDS             complement(10470..11387)
                     /codon_start=1
                     /gene="divX"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03798"
                     /gene_kind="biosynthetic-additional"
                     /note="Asp B-hydroxylase"
                     /product="DivX"
                     /protein_id="ARD09204.1"
                     /sec_met_domain="TIGR03798 (E-value: 3.8e-10, bitscore:
                     29.1, seeds: 22, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKETVIEFYEAIFESPEFIQEIKAITRQEELIKLGARNGYHFTM
                     EDLAQADASYIPKNNQPLISIDSDDRAREELPRPYHYEFEFSEIPGFEEIDRELKKLQI
                     KPNTVDLDLYEKSFREEDFKFNYISPTVPGFRQYYYKSLKSYLDLPSPQPEYAWRPFHL
                     INLDCHVEDPLYEDYFQTKVRLLKLLENCLETELRFSGSLWYPPNAYRLWHTNETQPGW
                     RMYLVDFDNFDDNQEGEVFFRYMNPETKELVTLAEKPKIVRFFKIESEPSKILWHCIVN
                     TTKLNRWSFGFNVSSKWMDKLLTV"
     gene            complement(11458..14712)
                     /gene="divM"
     CDS             complement(11458..14712)
                     /codon_start=1
                     /gene="divM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     704.7; E-value: 3.2e-213)"
                     /gene_kind="biosynthetic"
                     /note="class II lanthionine synthetase"
                     /product="DivM"
                     /protein_id="ARD09203.1"
                     /sec_met_domain="LANC_like (E-value: 5.9e-71, bitscore:
                     228.1, seeds: 47, tool: rule-based-clusters)"
                     /sec_met_domain="DUF4135 (E-value: 8.8e-79, bitscore:
                     254.4, seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDDFSLLKLASRASNLCEQTLIVKELAKSKAPIASTTQLSPVDS
                     WKIKKLTGKLAVQPFKESYEQGTISQSVIEDLRKLLIDYKLYELNLANLSESDRLEFIK
                     PHSQWLKAYQAAMATLDLPREKFSGSCWGEPDIYYGKFAKVCEPFLRLLHQTLRGTGDA
                     INATADNYRINPQVAIDIELHLLNRFELALAWALEANINVYCSQKAIAKSEDDSEAYIA
                     YLEETFDRKQNYHDFYCRFPVLARWLAQVTYFLCNFGEETLQRLTSDREQIGATFFGSK
                     PISQIKSFKLGKSDYHAGAKSVVIVELELANSEPATLVYKPRSIQSEAGMQGLLAQLNQ
                     DKVVRFAHYQVLCRDGYGYAEFIPSGKNQVQNKEDLKKFYQQLGGFLSIFHILGGGDLH
                     HENILVADGNAFICDCETVLEVLPQGMDKLPGTVLDSVFKTAMLDWPRDSASPENSEMM
                     SISGYSGGESYEVAFTVPRVKEHRMSLDQGVEYKTGITVELEGTNRIYYNGEIVDPQDY
                     KDSIVDGFNQVYTWFQQHPTKAITRIKELFSSSLVRFINWGTQAYAKSIVAVRHPKCLA
                     DPLEVDLIFNSLKEHKRQWDKKGELAELELGSLWQLDIPIFTALAAESKDLIFNYQYSV
                     SDTLAISPLDNAKRRIEQLSTENRIRQNQYIYTSLSTDEINSPYFIAAAVNYAQQIGWQ
                     LCEQLSSDSSKAPWQTWDYTATGKRLVDISGDLYDGSAGICLFLAYLDAIKPQVEFRQA
                     AERALEYSIEKRNTTLIGAFQGETGLIYLLTHLAQLWDKPALLDLAVDLSDELLPRIKQ
                     DIYFDILHGVAGIIPVMLGLAEATGGKGIDCALQCAEHLLEQGIYQDNTLSWPPGRPDL
                     VRGNFTGFSHGASGIGWALIMLGCHSNKSEYIEAGRQGFAYEATQFDEEQRDWYDLRKS
                     VTTADSNEPHFANAWCNGAAGIGLSRIISWAALGKTDDDILRDAYTALNATLRNFNKLG
                     NDSLCHGKSGNAELFLRFAQLRDTPYLQMEANVQAQAQWRNFEKARRWMCGSTGNDVFP
                     DLMLGLAGIGMHFLRLAYRERVPSPLLLDPPPRAID"
     CDS             14756..14854
                     /gene="allorf_14756_14854"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="allorf_14756_14854"
                     /protein_id="allorf_14756_14854"
                     /tool="antismash"
                     /transl_table=1
                     /translation="KNRHGSLFSFLSVQEKQLKLIWSKRDGKYCSKL"
     CDS_motif       14756..14782
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: KNRHGSLFS"
                     /locus_tag="allorf_14756_14854_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       14783..14854
                     /prepeptide="core"
                     /locus_tag="allorf_14756_14854_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-5.80"
                     /molecular_weight="2844.4"
                     /monoisotopic_mass="2842.6"
                     /core_sequence="FLSVQEKQLKLIWSKRDGKYCSKL"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="KNRHGSLFS"
                     /leader_location="[14755:14782](+)"
                     /alternative_weights="2862.4"
                     /alternative_weights="2880.4"
     gene            complement(15017..15292)
                     /gene="divA"
     CDS             complement(15017..15292)
                     /codon_start=1
                     /gene="divA"
                     /product="DivA"
                     /protein_id="ARD09202.1"
                     /transl_table=11
                     /translation="MPTTLEKPSVAYLEKLFHQTAIDSEFRSELQSHPEAFGISADLEL
                     PQSVEKQDESFVELLNNALGEIDIAAECASTCSFGIVTIVCDGTTK"
ORIGIN
        1 atagcagttg acagttgata gtggatagtt gatagttata cagcaacctg tataaatgct
       61 ggaactcaat agtaaacacg ttcataggcc ggtatcattg gtacatattg accaagaaac
      121 cctataaata tttttgcatc aatcttcaga ggaaataaaa aaattgtcta ggcaagaaca
      181 gctgaatcct tggaactata aaccttggtg gtgtcaacct tggtccatat tgttagttgg
      241 catatcgctg cagataggaa gctggattgt gttaaaaata gtatggctga caatcttggt
      301 cgtattactg gttttcgctt ggtggttcta ttttctcatc ctttggccaa agttagtaga
      361 taatattaga taactaaact aaaatggaat tttaactggt agcgccaagt gcaacaaaag
      421 cccaaaatta tctatccatt ctccccctag agcaagcaat tcccatgcaa aaccctgcgg
      481 cacagatcca atgctcaaat ctgaactgta aagaattaaa tccgcaaaac aagaagtttt
      541 gtcaacaatg caacacgccc atcagaaaac gctatctatg ggccatagga gaaggaatag
      601 aagctagctt ggtaggagat tacatcggcg atcgctattt actggtggat tctcgccttc
      661 ttctggatac caaaccgaca gaacggttca caatgactga ggatattcct caagcgatcg
      721 ctccctatct caaactttcg gattatagac tgcatattcc tagggtgtat ggagagttac
      781 aatccgcaaa tattaattct tcggaaatct ggctgctaga atacgatggg attataacag
      841 aagagagaga ccaatccgaa gaaataatac tgctgccaaa gatagacaca gtttggcaaa
      901 aagcaacggc cttgcgccag ttaaactggt tatggcaaat agcttccctt tggcaaccgc
      961 tgcatcggca aaaagtagtt tccagtttac tcaatccttc ccttctacta gtagatcgct
     1021 ccatagtaaa gttgcaggaa ctaagagcgg atggaaataa aaccatcagt ttaaaaaatt
     1081 tgggtcaact ttggagaaaa tgggctgatg agtcgtcaga aacaatcaaa gaatttttaa
     1141 caaaactaat agagcgcctg gaaacaggag aaatagcaga agcaagggaa gtggtagcct
     1201 atctggagct agcaatgcaa aaggtagggc aatctcaaca gcgaacttat agtactttca
     1261 cccaaactca ttcgggaccg actcgggacc ataatgaaga tgcttgttat ccttcaacca
     1321 atactgaggc aatctccaaa atgtcggggg ataaccctat cgctatagta tgtgacggta
     1381 ttgggggaca agaaggggga gaaatagctt ctcagatggc cattgatttc ttgggagata
     1441 atctagaaaa aatccaccca gactcagaaa catggaatcc cattgagatt cagcaacagt
     1501 tagcaacatt aacctgtgag gccaacgata ccattagctc ccgcaacgat cgtgaacagc
     1561 gttacaaccg agaacgcatg gggactacct tggtaatgag ttttgctcat ggccatgaag
     1621 tttatcttac tcatgtagga gattctcgca tttattggat aactcccagc aattgtcatc
     1681 aagtaacagt ggatgacgat cttgcttcca gacaagtgcg attaggttat gctctttatc
     1741 gagatgccaa taattatccc caagctggag ctttaattca agcattggga atgaattcct
     1801 ccacaacttt atatcccaca gtacagcgat taataataga tgaagacagc attttgctgc
     1861 tctgttccga tggtttgagt gactttgacc gagtagaaca atactgggag aacgaaattg
     1921 taccgattct agaagggacc acagatctag ttacagtagg aaagcagtta atcaaaatcg
     1981 ccaatgaaaa aaacggtcac gataacgtta ctgtagctct agttcactgt caagtggagc
     2041 caactgaagc agaaacagca gcaacagcac tctcctggtc agagattgaa ttgggtgtgg
     2101 agcaattatt aattagccaa aacaatcaaa cagctggaat agcaaccgag tccccatcac
     2161 ctgagcctct cgattcatca aaagaaaaaa accggagatt attattttta ttaacaacta
     2221 tatttatttt gataataggg ggtttattaa catttaaatt taaagaagaa attaaacgga
     2281 aagacgagct actaaacagc cccaactcgc aatcttcctc taatctggtt gtatctctgg
     2341 gggatgagaa taaaatagtt gacagttgat agttgatagt tatacagcaa ccaaccagtt
     2401 gctttcactc aagactaaat gtctgtaagt aataatcttt gcctcagtct agccatcgcc
     2461 cgtctgaccg cagcaggacc ggacaatttt gctatttgga caatggaatc tccattacca
     2521 ggaggctacg ttcaccacga ttctacctgg cctgtttctt tgactcagaa atggttggca
     2581 tggcaggaaa tgttttctct gcaaaatcaa cctcatctac ctatggaagg gaggaccgtt
     2641 ttaccaaatc cagagaatgt tcctgtttat ccaactgggc caaactatgg cggaagattg
     2701 atgcaggatt tgggtattaa tctttggcag tggctctttg atggtgccat tcgtcatagc
     2761 ctggatcgca gcgaaggaat cgcgatcgga caaaatcaac ccctacgact cagactggaa
     2821 attcgcgatc ctaatttgat tgctttacct tgggaaatta tgcaacaagc tgctggcaag
     2881 caggcaattt ctctcaatca gcaactgctg tttagtcgca ctaccagcga tgtggatccc
     2941 attagccaaa agctccatca ggaagtatta aacatcttgc tagttttggg aagcaatgtt
     3001 gatggagaaa atttgcagct tcagcaagaa gccaatacct tagcaagaga gatagaagca
     3061 agagaggtcc aaacaaacac cattgttccc ccagttcctg ctcgtgtaga tgttctggaa
     3121 caaccaagtg cagaagaact aatccaagcc ttagaaactg gtctttataa cgttatctgt
     3181 tatagcggtc acggtgttac ctccagcgac ggaggtttgc tcctcttgcg atgggatgct
     3241 accctcaacg gcacggaatt agctcaggtt ttggttcgca atcaaattac tttggcagtg
     3301 tttaatgctt gttggggggc tcaaccagat tctcacaagg gaaaaacaat agaacgcagt
     3361 agtttagccg aagtgctgat tcatcatggc gtacccgcag ttttagccat gcgagatgaa
     3421 atcgccgatc gcgaagcatt gagttttgtc agcgcttttg ccaaagccct ccgacaaaga
     3481 atggccgtgg atggcgctgt agtggtagca agacaaaaat tattgactct ttataaattt
     3541 aaccaacctg cttggacatt acccattctc tacatgcacc cagagttcga tggagaattg
     3601 attaaaccca ttaacgaggg agtaacagaa ctgcccaact taccaactag cttgaggaat
     3661 aaatccctac ccctagctta tcttcgttcc ctcaaagaga aagataaaag ctggccaatt
     3721 cggggtggga ttatgcgagt tggcaggggt cagggtaacg atttgatggt tttggaaaag
     3781 tgggtgagtc aacaacatgc ggagattatt tgtcgagaaa tagctgaaga tttaaattac
     3841 caaaattgct actttcttcg ggatttttct cgttttggca ctttaattta tttagatggt
     3901 ggatggcgca aaatttatca tcaggaagtt tctttagaat ctggagttca gattaggttt
     3961 ggtagcgact acggtgaaat ttttgagttt ttgattgatt gattgatata tttgtaaccc
     4021 acattccgca cctcgtgttg tggcacgaaa tgctacgcgc gatcgctaca gggtggtgaa
     4081 acttttcaaa cacgctctaa gatagagatt ctatagtaaa aaatattaat ttttgttaaa
     4141 caattcaaca cttctgggtt tgagacacct ttaagcctat agtctaggaa aaagaggttg
     4201 aattagagcc aacttggtca tctgaaaccc tcatgctgtc gtttccccct gaacagttac
     4261 gaaactacga agatagagaa gctttatgta taaaggtcta gatagagatg aaattagaca
     4321 aatacaggtg ttaatgttac tgtgtctgtg tctctcaccc cagagcaagc tacgtcaatt
     4381 gttggaaata gcgttagccg cttctgaaac tcagataatg actaggatga ctccctgtga
     4441 tgatgtgaat gtggatggtc tgtttacttg ggtacagtct ttgttcgcgc aaggagggtt
     4501 gacagaggag gaaaagcgtt tgctgaaatg gcaaaacgaa tctcgtaata tgttgcctgc
     4561 cattgatgag cttaaaacca ttgaaaagaa gcttggcttt aagattagaa tacagaaact
     4621 acagtctcat aactaggttg tgttgacatt tcagtgtaac aaaatgagag ccgcgatgaa
     4681 atagataaaa ctgaggaagt tcttcacagt cttctcaaaa gagggagatt ttagatggag
     4741 tgttgtctca gctcaagaat ggttgcaact ggtgcgattt gccctgagat ttacctccct
     4801 actccacagt gttttggcac tacaaacagt ggcgagccga aggagtcatc gagagcattc
     4861 cagtgtggcg gtaacggagc gctatcttca tgctcttgcg acggagagtt cttcagtttt
     4921 gtcagtcaac cagcctagaa aacgcacaca acccgaaacc ccaagcagtt gtacctggaa
     4981 tcagcttcgt tcgggcggcg acaggcagaa cggcaatatc tagggtcgtt aagccaggaa
     5041 ccaccacgcc ccggacgatg tagacgatcg ccaccctcaa gccacacact accatcagtt
     5101 ggtgaatcag tgtattcgtg atgccaaaag tcagcgcacc actcccagac gttcccgtgc
     5161 atatcacata gtccaaagac attgggtgga aaactaccta ctgtagtcgt ttcttggcga
     5221 tattctccct cgggacccga accataatgg tcaccattgt agttagccag gtcagtggta
     5281 attgtctctc cacagtgaaa tggtgcattc gttccagctt ggcaagcata ttcccactcc
     5341 gcttcgctag gcaaacggta ctctcgccct gtttttctgg aaagtctctc gcaaaactcg
     5401 acggcatcgt accaggatac ttgctctact ggtctactcc ccctagaact gggggggtct
     5461 ttaaaccaag agggatcggt gtcgaggtgg cgattgcttt ttggcagggc tgcaacagct
     5521 ctccactgcg cctgagtgac ttgatactta cccatgaaaa aaggctctag ggtgactttg
     5581 tgatgaggac gttcataagc ccctgcctct ttgtttgtcg aacccatcac aaattcgccc
     5641 ccagggatag agatcgcttc taaagtgacg ccactgccta gatcttcggt aaagtatcga
     5701 gcttgactgc ggcgacggct agctttccta cctctcgcat ctacagtcac cacgtcaaat
     5761 tcaaacacct gcaacggtgg agtagtcatc attgtttcct gacttcttta gaataaccaa
     5821 cttgtaaata gtatttcata aattgacggc cacaattaaa gcaattgata gttttgtttt
     5881 cctctatgat ggccattttt tataatctca tgagaaccac atttgggaca attaatggtc
     5941 attttagtta gttagcgtcc ccctgcggga ccgcatttta taatttcctc ttttattata
     6001 caccatcccg cagggatgca acgccaagtt gttggctggg ttattctctc gataaaagtt
     6061 taacaagtat gcgagattta tttaagagat tgaatggaac ggatataaat gggtcgcaat
     6121 tgggctcgca gttggttaat ctgtttcatg agggtttttg gatgttgtgg tgatttccat
     6181 caaaccctct tgccctaact caatacaacc ttcgccactt ttgtcctgta cccaggactc
     6241 aagttattct aaactccagc ttatgagtga aacccataga tttgaccgtc agttttggca
     6301 acgattttta gcccttgccc aaccttactg gtacccaata tccggcagcg gagtagcgtt
     6361 tctaggactt ttgctgttac taatgtccct cctgttgacc gttagtttta tagctgtcag
     6421 tatggtcagc ttgctcggtc agtcttactt cccgaacgtc ctcaatagta ttgctccagg
     6481 cttagcagaa tccatcctca agattatcca atcgccagcc atctatcttg ttgccctctg
     6541 tttcccagtt acgctcgtca gctttgttta ttacaaaaga aagttacaag agcattggcg
     6601 accgtggtta atcttgacat tgctgctact gttgttgtta gggagcattg gcatgcaagt
     6661 tctcaacagc tatttgggtc gagacttaat caccgccatg gctcaaggca aggagtcaag
     6721 ctactttctc ctcctgttgc tctatggcgc ttctctaatc tttgtcgtac ctttggtggt
     6781 cttatctcac tacgtcagaa aaaaactctc aattcactgg cagcaatggt taaccaatga
     6841 gttcctcgct aaatatttct atcagaaagc ctattatgac ctcaaaaccg atctcaagat
     6901 tgacaatcca gaccagcgtc tctctaaaga aatcgagttt tttacccaga ccattatgga
     6961 ttttgtggtc acgattttca accaactgat taacctaatt gcttttggga tcgttctctg
     7021 gtctatttct aaatacctgg ttgcgatttt aatcgtctat gcggtggtag gcaatctgat
     7081 cacggtaatt tccagccgac gattagtcga tctcaatacc gataaacttc aatacgaagc
     7141 gaatttccgc tacagcttaa ttcgagtcag cgacaatgct gaatctattg ccttctctcg
     7201 cggcgaggat cgggaattat ctttggttaa gcagcgattt acgcaattgg tgaaaaatat
     7261 taatcgcatg attggctggg agcggaatat agaactctta agcgttggct atcaaaatat
     7321 cctcttcatt atcccgttct tggtggtagg ccctttgtat ttcagcggtc aagttgagtt
     7381 gggagtgatt acccaagcaa gtatggcctg cgctcagcta tccgctgccc tgtcagtgat
     7441 tgtcagtcgg tttgaatctc tcagtgccct tgctgctgta gttaatcgtt tagccacctt
     7501 cacagaggct ttgacagtct cagagacaac agaaaagtct gtcaacacca tcgagccagt
     7561 ggaagacaac cgctttgcaa tagaagcact cactttgcaa actcccaata gagaacagac
     7621 cttgctcaaa gagctgtcgg tgtcagtaga accgggtgaa aatgtcctga tcgtgggggc
     7681 tagtggctgt ggcaagagtt ctctgctacg ggcgatcgcc ggtttgtgga acgcaggaac
     7741 gggtcgcatc ttgcgaccca acctggaaga aattttgttt ttgcctcagc gtccttacat
     7801 gattttgggg tctttgcggg aacagttact ctaccctaat ggcgatcgcg aaactcctga
     7861 tgataaacta gagtcagtgc tgcatcaagt taaccttccc cagttgcttg gcaaagtggg
     7921 aggctttgag gcagaactgg attggagtaa cgttttgtcc ttgggagaac aacagcgtct
     7981 ggcctttgca agactcctgc tcaatcgccc tcgctacgcc tttttggatg aagcaaccag
     8041 tgccttggat ctgcaaaacg agaagcatct ctaccaacag ctgcaataca gtaatattac
     8101 ttttgtcagt gtcggtcacc gagaaagctt gttaaactac catcagcggg ttttgaaact
     8161 attgggtgat gctaattggc aacttttttc ggctatgcta tgattattag ttgactggct
     8221 ttacagatat ctaggaatta cgcattgaac taaacgacaa ggatagaatg gggttttcag
     8281 ctcccttcaa attgacgtga atgagtaatt atgctaccta tcatatcatg tccggtagta
     8341 ttgtcattgt ataattatac atcgtcaatt taattggcat tgccccctcc cccccttggt
     8401 cccccccact gggggggaaa caggggggga ggggactttg aggagttttc ccccaatgtt
     8461 gggggttagg gggctagaca aattataggt atgatatttt cggacatgat atcatctcct
     8521 aaaataactc ccagcctatg ctttataaac atctttcttt acaaaaaatt tgacaaaagc
     8581 gcattttgtg tatattgggc tgaaatgacc atttttggta gcgagcgcca acaacattgt
     8641 gggattttta caaatacatg gttttgtcct tttgtcaagt agtaatagtg gaataaaagg
     8701 ctatatggct tttaaaacaa ggattacagc ttttgttaag aaagtttctt attcctattc
     8761 ctacggggtt atagacctaa acagcaatac cgacagcatg accgtattga tttgtacaat
     8821 atatttttag ctcaaagccc cctgcctgta actcgctggc aatttcatcc ttcgtgaagt
     8881 agtgaacgta attgggagct aaattatctc ctacttctaa gcattctctt cctagcaaac
     8941 gtctgaatgc attgccaatc atagcgatcg ctttaaaatc gcgtccacct gtagtttcag
     9001 agtaacaaaa gaaagaaaga aggacaggag aatttttttt agcttgagta cgtagttgcc
     9061 tcagaaattc gattcttcgc tcttttccct gaattaacat gtaagctccc caaccaacaa
     9121 tcagtccatc atattctttt tgactatctg gacattgatc tcttggagct aactttatat
     9181 gagaagcaaa ttcttcttgt ttaatcaaat tgttggcaaa tttaagcaaa ttggcattgc
     9241 attcaaatcc gtcaacttca tatcctcttt tacagagtgc taaaacttct ctgccacctc
     9301 ctgcagccac tactaataga ttatgacact gctgaaagta ttgagctaaa actttttctt
     9361 cccaatccca caatcctcgt ttattataat tttcatctcg atacatctca gtttggttat
     9421 aataaatact atcaactaaa tgataatgct ctcttttgag aactcccaac caaaacccaa
     9481 taaaaaaagc attcaatgca aagattattt ctttgaaaat tttaaaagaa aaatcgtagg
     9541 cttttaactc aaaaggataa ttattgtttt ccattgcgat aattctaatc tctatctgtc
     9601 ttttataaag cagcagataa gcctaaacca atcctgtcgg gtatggttta ggcgaggtac
     9661 aatttacttg ttaaagggat taattccgaa tccgcatgag aagtttgatg gcatcaacat
     9721 taccatccat acgagaatcg ctgaaaattt gattgagaaa gtcctcagtc gccttaaccg
     9781 ctaaggcgcg tgcggtagga aatccagggc gactgttatc gtctttgttg agtccatcag
     9841 ggcatacgat gggcacgcca tggcggcact ttccttcagg aacgtcacag gagggtacta
     9901 ccccaataaa ggtgaaatat ccagaagtca attcagttaa gccagcaccg ccgagaatcc
     9961 ccggatcgtt gggacaggtt gccgtgtttt tgtctgctcc agagaagact tcccgaccta
    10021 ttttagtatt gatatcggag ctgctatgac cgagttctac ccaattactg tgagcgtaga
    10081 agtcttggac agtatgtaat gctgtaccca aatcgttacg ggctgaggtt ccttggggat
    10141 cttgggggtc agtcactttg gtaatggttc gttcttttag gaacatcacc cggttgctac
    10201 caccagaaaa gtcttcacca tcgaaatgca tctcggtatt aaactgattg ggaagatcgt
    10261 ctgtgtcctt atttgctttc acaatttggt ctatggcata atcggtgaat ttaaacgtct
    10321 cgccttcaac tactacttga aaatcctcaa acacctctct ggttatttct tcgtgtatcg
    10381 gaaccttgaa agcaaaggct gaagatggca ttcctaacag tagtgctaat gccaacaagc
    10441 aagttaaaaa aattttcatt ttattctcct taaactgtca ataatttatc catccattta
    10501 ctggaaacat taaaaccaaa actccacctg tttaatttcg tagtattaac gatgcaatgc
    10561 cacaatattt tacttggttc gctctcgatt ttaaaaaatc taacgatctt gggtttttca
    10621 gccagggtta ctagctcttt tgtttcggga ttcatatagc gaaaaaacac ttctccctct
    10681 tgattgtcgt caaaattatc gaaatcaacc aaatacattc tccaacccgg ttgggtttca
    10741 ttggtatgcc acaatcgata agcgtttggt ggataccata agcttcccga aaacctaagc
    10801 tcagtttcca agcaattttc tagaagcttg agaagcctca cttttgtctg aaaataatct
    10861 tcatagaggg gatcctccac atgacaatcc aggttaatta agtgaaaggg acgccaagca
    10921 tattcaggtt gaggagaagg gagatccaaa taggatttta atgatttgta ataatactgc
    10981 ctaaatccag gtacggtagg agagatatag ttaaacttaa aatcctcttc tcgaaagctt
    11041 ttttcataca aatctaaatc cacggtattg ggcttgatct ggagtttttt caactcccga
    11101 tctatttcct caaaccctgg aatttccgag aattcaaatt cataatgata ggggcggggt
    11161 aattcctccc tagcacgatc gtcagagtca atggagatta atggctgatt atttttggga
    11221 atataagaag catctgcttg agctagatct tccatggtga aatggtagcc atttcttgct
    11281 cctagtttaa ttagctcctc ctgccttgta attgctttaa tttcctgtat aaattctggt
    11341 gactcaaata tagcttcata gaactcgata acagtttctt ttgacatctc gatcctaata
    11401 aacaaactaa actatattac tcttgggtgt tgaatattga attaaaaaac tcaaaactca
    11461 atcaattgcc cttggtggtg ggtccagtag cagaggcgaa gggacgcgct cccgataagc
    11521 tagacgcaaa aaatgcatcc cgatacctgc taaaccgagc atcaggtcgg gaaagacatc
    11581 gttacctgtg gaaccgcaca tccaacgacg agctttctcg aaattacgcc actgtgcctg
    11641 agcttgcaca ttggcttcca tctgcaaata aggtgtgtct cttagctgcg caaaccgcaa
    11701 gaatagctca gcattacccg atttgccatg acataaagaa tcattgccga gcttgttgaa
    11761 atttcgcaaa gtggcattta aggctgtgta agcatcccgc aagatatcgt cgtcagtttt
    11821 ccctaaagcc gcccaactaa tgatacggct cagaccaatc cctgctgcgc cgttgcacca
    11881 agcgttagca aaatgtggtt cgttggagtc tgctgtcgtg acagatttcc gtagatcgta
    11941 ccaatcccgt tgttcttcat caaattgggt cgcttcataa gcaaaccctt gacgaccagc
    12001 ttctatatat tcacttttat tgctatggca accgagcata ataagcgccc aaccaatgcc
    12061 gcttgccccg tgggaaaagc cagtaaaatt gcctcgtact agatccggtc gtccgggagg
    12121 ccaactgagg gtattgtcct gatatatccc ttgctctagc aagtgttcgg cacattgcaa
    12181 ggcacaatct atgcccttac caccagtagc ctctgctagc cctagcataa cgggaataat
    12241 accagcgacc ccatggagaa tgtcaaagta aatatcttgc ttaatacgcg gcagcagctc
    12301 atcactcaag tcaacagcca gatccaacag ggctggttta tcccatagct gagctaggtg
    12361 cgtcaacaag taaatcaacc cagtttcccc ttggaaagcc ccaatcagtg ttgtattgcg
    12421 tttttctatg gagtattcta aagctcgctc tgctgcttgg cgaaattcta cctggggttt
    12481 gatggcatcc aggtaagcca aaaatagaca tattcctgca gagccgtcgt agagatcgcc
    12541 actgatatcc accaggcgtt ttcccgtggc ggtataatcc caggtttgcc agggagcctt
    12601 gctagagtca gaggaaagct gttcgcacaa ttgccagcca atttgctggg cataattaac
    12661 cgctgcggcg atgaaatagg ggctattaat ttcgtcagtg gacaaactag tataaatata
    12721 ctggttttgt cggattcggt tttccgtaga taattgctca atgcgccttt tggcattatc
    12781 cagtggcgaa attgctagag tatcactcac tgagtattgg tagttaaaaa tcaaatcttt
    12841 actctcagct gctaaggcag taaaaatcgg aatatctaac tgccacaatg accctagttc
    12901 caactctgct agctcaccct tcttgtccca ctgtctctta tgttctttta aactgttgaa
    12961 aatcaaatct acttccagtg gatcggcgag gcactttggg tgtcgcacag caactattga
    13021 tttagcgtaa gcttgggtgc cccaattaat aaaccgtacc aatgatgacg agaacaactc
    13081 tttaattcgg gttattgctt tggtaggatg ttgttgaaac caggtataaa cctggttaaa
    13141 gccatccaca atagagtctt tataatcctg gggatctact atctcaccgt tgtaatagat
    13201 acgattcgta ccttctagtt caacagtaat accagtttta tattctactc cctggtctaa
    13261 ggacatgcga tgttccttaa ccctgggtac ggtaaatgct acctcgtaag actcgccgcc
    13321 gctgtagcca cttatactca tcatctcgct gttctcgggg ctggcactgt ctcgaggcca
    13381 atctagcatg gcggtcttaa aaactgaatc aagcactgtc ccgggcaatt tatccatgcc
    13441 ctggggcaac acctctaaaa ccgtttcgca gtcgcaaatg aaagcattac cgtcagcaac
    13501 aagaatattt tcatggtgca agtcgccacc acccaagata tggaatatgc ttaaaaaacc
    13561 acccagttgc tggtaaaact ttttgagatc ttccttgttt tggacttggt ttttaccgga
    13621 gggaataaac tccgcgtaac catagccatc tctacaaaga acttgataat gagcaaatcg
    13681 aacaaccttg tcttgattca attgtgctaa caaaccctgc atccctgctt cagattgaat
    13741 ggagcgaggc ttgtatacga gagttgctgg ttctgagttg gctagttcga gttcaactat
    13801 gaccacactc ttggcaccag catggtagtc agatttacct aacttgaaag atttaatttg
    13861 tgagataggt ttactgccaa agaaagtagc accgatttgc tcgcgatcgc ttgttaaacg
    13921 ttggagggtt tcttcaccaa aattgcatag gaaataggtg acctgagcta accaacgtgc
    13981 tagcacggga aatcggcaat aaaaatcatg ataattttgt tttctgtcaa aagtctcttc
    14041 caggtaggcg atatacgcct cggaatcatc ctctgatttg gcgatcgcct tttgagaaca
    14101 gtaaacattt atatttgcct caagagccca ggctaaagca agttcaaaac gattgagcag
    14161 atgaagctca atatcaattg ctacttgcgg attaattcta tagttgtctg cggtagcatt
    14221 gatggcatcc ccagttcctc gcaatgtttg gtgcaacagc cgcaaaaatg gctcgcaaac
    14281 tttggcaaat ttcccatagt aaatatccgg ttctccccag caactccctg aaaatttttc
    14341 tcttggtaag tctagagttg ccatggctgc ttggtatgct ttcaaccatt gactgtgggg
    14401 tttgatgaat tctaatctgt ccgattcaga taagttggct aaatttagct cgtataattt
    14461 ataatctatg agcagttttc gtaggtcttc aattaccgat tgactaattg ttccttgctc
    14521 gtaagattcc ttaaatggct gtacggctaa tttacctgtg agtttcttta tcttccagct
    14581 atcaacagga ctaagctgtg tagtggatgc tatgggcgct ttgctcttag ctagttcttt
    14641 gacgatgaga gtttgctcgc ataagttgct agcacggctg gcaagtttaa ggagagaaaa
    14701 atcatcaagc atagtggagg ttctcagagt aaaatttgta ttttgtttga gcaacaaaaa
    14761 cagacatggt tcattgttct cctttctctc tgtacaggaa aaacaactga agctcatatg
    14821 gagcaagaga gatggaaaat attgctcgaa actttatgag ccaatagttt cggacatttt
    14881 actacgcttc tcatccaagt tttgtcttgt acttagttcc tttctaacca tgcccaacaa
    14941 tcaatttaat gaggctagct tcacaatgtg gtgctagccc agacatatta ataatataat
    15001 atgctagtaa aacaacttac ttagttgtac catcacagac gatagtaaca ataccaaagc
    15061 tgcaggtaga tgcacattct gcagcgatgt ctatttctcc caaagcattg ttgagtaact
    15121 caacaaagga ttcatcttgc ttctcaactg attgaggtag ctccagatct gctgagattc
    15181 caaaagcttc aggatggctt tgaagttcgc tacggaactc ggaatcgata gctgtttgat
    15241 ggaacagctt ttctaagtat gctacagacg gtttttctag tgtagttggc attagatttc
    15301 ctcgaaagaa tgaacttgat catagcttaa accacatttg gcatttttag agggaaatat
    15361 ttacaattct taatcttttt tttttcgaca atgaaatctc acagggtgac ttgtcacccc
    15421 aaactcttat cttaaaatgg tttcaggcat tgagctgaag ccaaaaatca taatgaagct
    15481 ggtgataatg aagctgatga tgatgataac tcacagggtg acaacaccgt tgtcaccctg
    15541 actgagcttg agtttaaaca ggattgggtt ggagaaaaat taggtaactg ttgggagctt
    15601 attgacaaag cgcactcttt gcggatacct ttggtatcgg ggaaaaggcg atcgctactc
    15661 ttcttgatcg ccgaaataaa aagcagaaga tctgagcagc gatcaccctc atctgcagga
    15721 gatcttgcag tggcaggttt cgccggtcgc gcaccgccag cgaagcctat ctcagctata
    15781 aatgccattt tctatttgat attagtgaaa aagtcccgga aaagagaata aaaaaaatgt
    15841 tcgatttaat tttttacccc agcaacaacg aggaaccaga atatatagaa gtgtcggcag
    15901 aaatgtacga gtggctcgct aaatcggagt tctctaaaac cggcaaatct gtgacgagaa
    15961 caatccacat cgatggagag gaggaaaaag tcccagtggt acaattaaat caagaaaatc
    16021 gtcaaagact gcggaatttt tttatcgaac caataattca agaaagcgat gcagtcttta
    16081 aaaaactggg ggatatcccc gagaaacaag aatatctgga aactacttat cgtttgagaa
    16141 agttgctcca attacgtcaa tgtatcgaga acgaaaatta ctactatctt cagagagtac
    16201 aataaacagc agatgaagtc acaggcaaga ttgaagtcac aggcaagatt gaagtcacag
    16261 gcaagattga agtcacaggc aagatgcctg tgccacgatg aacgcagata aagggggata
    16321 gatttgagta gcgatcggtc acggatccgc aaagagtgcg ccttgtttct caaaagcaca
    16381 agtctgagca gcgcacagct tcgcggatct caaagatatc gctcttcatc tgctgcaggc
    16441 gctctcagct ataaatgcca tggtgggcaa tgcccaccct acgaagagac tcaattgttt
    16501 aaaaaagaag ttccatctca aacaaccaat aaccaatcaa aaaaatccct atatatatgg
    16561 cggcacattt tcccactcct aaactctaac atctcataac aaaaaatgca agacgagttc
    16621 aactaaatcc aaaaaatttc tgctcggaat ccagattcat tactctatcg ttagattaaa
    16681 acccccttta actctaattt tccagctact ttatcctctc aaacctataa atactcagaa
    16741 aaacttcgca gaaacccaag aactagcttt gg
//
