BGC0001597 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001597 (Actinomadura fulva subsp. indica)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	71,515	fluvirucin B2	PKS	100%

BGC0001597 - Region 1 - T1PKS

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Location: 1 - 71,515 nt. (total: 71,515 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 71,516
Label		BGC0001597

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001597	0.99	Polyketide	fluvirucin B2	Actinomadura fulva subsp. indica
BGC0000016	0.39	Other	amphotericin B	Streptomyces nodosus
BGC0002047	0.37	Polyketide	brasiliquinone A, brasiliquinone B	Nocardia brasiliensis
BGC0001817	0.35	Other	vancosamine	Streptomyces sp. WAC2288
BGC0001925	0.33	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0001163	0.32	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000265	0.32	Polyketide	rhodomycin	Streptomyces purpurascens
BGC0001164	0.32	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001965	0.31	Other	nybomycin	Streptomyces albus subsp. chlorinus
BGC0001651	0.30	Other	legionaminic acid	Tannerella forsythia 92A2

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001597	1.00	Polyketide	fluvirucin B2	Actinomadura fulva subsp. indica
BGC0000016	0.71	Other	amphotericin B	Streptomyces nodosus
BGC0001896	0.70	Other	carbazomycin B	Streptomyces luteoverticillatus
BGC0002047	0.69	Polyketide	brasiliquinone A, brasiliquinone B	Nocardia brasiliensis
BGC0001817	0.68	Other	vancosamine	Streptomyces sp. WAC2288
BGC0001925	0.66	Alkaloid	altemicidin, SB-203207, SB-203208	Streptomyces sp.
BGC0001163	0.65	Polyketide	1-heptadecene	Moorea producens 3L
BGC0000265	0.65	Polyketide	rhodomycin	Streptomyces purpurascens
BGC0001164	0.65	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001965	0.65	Other	nybomycin	Streptomyces albus subsp. chlorinus

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 2508 - 71515:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal - ohmal) + (Me-redmal - redmal) + (Me-redmal)
Non-colinear ordering:: flvP1 + flvP2 + flvP3

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

flvK

PKS_AT (20..292): pk

ATSignature: (unknown)

No matches above 50%

minowa: CHC-CoA

Prediction, score:

CHC-CoA: 13.6
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

flvM

Search NORINE for peptide: strict or relaxed

AMP-binding (16..408): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DCFFFALITK
Nearest Stachelhaus code(s):: Ala DCFFFALITK (100% 8Å match); bMe-Asp DCFFFALITK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

flvP1

PKS_AT (758..1055): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 83.3%; Methylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 158.8
Ethylmalonyl-CoA: 145.5
Methoxymalonyl-CoA: 133.8
2-Methylbutyryl-CoA: 91.9
Propionyl-CoA: 91.3
trans-1,2-CPDA: 86.3
Isobutyryl-CoA: 77.1
Malonyl-CoA: 76.5
CHC-CoA: 67.7
Benzoyl-CoA: 58.0
inactive: 36.6
Acetyl-CoA: 35.8
fatty_acid: 35.6
3-Methylbutyryl-CoA: 19.9

PKS_AT (2856..3149): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 168.2
inactive: 79.0
Methoxymalonyl-CoA: 64.0
Methylmalonyl-CoA: 38.9
Propionyl-CoA: 27.1
Acetyl-CoA: 23.1
Ethylmalonyl-CoA: 16.3
Isobutyryl-CoA: 15.3
trans-1,2-CPDA: 15.2
fatty_acid: 13.3
Benzoyl-CoA: 12.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

flvP2

PKS_AT (583..880): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 83.3%; Methylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 158.8
Ethylmalonyl-CoA: 145.5
Methoxymalonyl-CoA: 133.8
2-Methylbutyryl-CoA: 91.9
Propionyl-CoA: 91.3
trans-1,2-CPDA: 86.3
Isobutyryl-CoA: 77.1
Malonyl-CoA: 76.5
CHC-CoA: 67.7
Benzoyl-CoA: 58.0
inactive: 36.6
Acetyl-CoA: 35.8
fatty_acid: 35.6
3-Methylbutyryl-CoA: 19.9

PKS_AT (2688..2981): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 169.4
inactive: 80.1
Methoxymalonyl-CoA: 64.6
Methylmalonyl-CoA: 39.7
Propionyl-CoA: 26.5
Acetyl-CoA: 23.1
Ethylmalonyl-CoA: 16.0
Isobutyryl-CoA: 15.3
trans-1,2-CPDA: 15.0
fatty_acid: 12.8
Benzoyl-CoA: 12.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

flvP3

PKS_AT (651..948): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 83.3%; Methylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 158.8
Ethylmalonyl-CoA: 145.5
Methoxymalonyl-CoA: 133.8
2-Methylbutyryl-CoA: 91.9
Propionyl-CoA: 91.3
trans-1,2-CPDA: 86.3
Isobutyryl-CoA: 77.1
Malonyl-CoA: 76.5
CHC-CoA: 67.7
Benzoyl-CoA: 58.0
inactive: 36.6
Acetyl-CoA: 35.8
fatty_acid: 35.6
3-Methylbutyryl-CoA: 19.9

flvN

Search NORINE for peptide: strict or relaxed

AMP-binding (39..433): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DYYPTTMRVK
Nearest Stachelhaus code(s):: Asp DYYPTTMRVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)