LOCUS       BGC0001605              9118 bp    DNA     linear   CON 11-MAY-2023
DEFINITION  Sinorhizobium fredii NGR234 plasmid pNGR234a, complete sequence.
ACCESSION   BGC0001605
VERSION     BGC0001605
KEYWORDS    .
SOURCE      Sinorhizobium fredii NGR234
  ORGANISM  Sinorhizobium fredii NGR234
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            U00090.2.
            On Aug 12, 2009 this sequence version replaced NC_000914.1.
            Rhizobium spec. strain NGR234 is available from Prof. Wolfgang
            Streit, Biozentrum Klein Flottbek,Mikrobiologie, Ohnhorststr. 18
            D-22609 Hamburg, wolfgang.streit@uni-hamburg.de.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 15:54:06
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1203
                     /GO_function="GO:0020037 - heme binding [Evidence IEA];
                     GO:0005506 - iron ion binding [Evidence IEA]; GO:0016705 -
                     oxidoreductase activity, acting on paired donors, with
                     incorporation or reduction of molecular oxygen [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 360.6; E-value:
                     1.6e-109)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532617.1"
                     /locus_tag="NGR_RS31335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGR234_213"
                     /old_locus_tag="NGR_a02700"
                     /product="cytochrome P450"
                     /protein_id="WP_010875295.1"
                     /sec_met_domain="p450 (E-value: 1.2e-33, bitscore: 105.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEQPLPTLPMWRVDHIEPSPTMLALRANGPIHNVRFPRGHEGWW
                     VTGYDEAKAVLSDAAFRPAGMPPAAFTPDCVILGSPGWLVSHEGGEHARLRTIVAPAFS
                     DRRVKLLAQQVEAIAAQLFETLAAQPQPADLRRHLSFPLPAMVISALMGVLYEDHAFFA
                     GLSDEVMTHQHESGPRSASRLAWEELRAYIRGKMRDKRQDPGDNLLTDLLAAVDRGEAT
                     EEEAIGLAAGMLVAGHESTVAQIEFGLLAMLRHPQQRERLVGNPSLVDKAVEEILRMYP
                     PGAGWDGIMRYPRTDVTIAGVHIPAESKVLVGLPATSFDPRHFEDPEIFDIGRDAKPHL
                     AFSYGPHYCIGMALARLELKVVFGSIFQRFPALRLAVAPEELKLRKEIITGGFEEFPVL
                     W"
     subregion       1..9118
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001605"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9118
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..1203
                     /locus_tag="NGR_RS31335"
                     /old_locus_tag="NGR234_213"
                     /old_locus_tag="NGR_a02700"
     misc_feature    283..285
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1296..2699
                     /locus_tag="NGR_RS31340"
                     /old_locus_tag="NGR234_212"
                     /old_locus_tag="NGR_a02710"
     CDS             1296..2699
                     /GO_function="GO:0020037 - heme binding [Evidence IEA];
                     GO:0005506 - iron ion binding [Evidence IEA]; GO:0016705 -
                     oxidoreductase activity, acting on paired donors, with
                     incorporation or reduction of molecular oxygen [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 311.7; E-value: 1.2e-94)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875296.1"
                     /locus_tag="NGR_RS31340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGR234_212"
                     /old_locus_tag="NGR_a02710"
                     /product="cytochrome P450"
                     /protein_id="WP_010875296.1"
                     /sec_met_domain="p450 (E-value: 8.3e-25, bitscore: 76.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDMQETTTACADAFAELASPACIDDPYPFMRWLREHDPVHRAASG
                     LFLLSRHADICWALKATGDAFRGPAPGELARYFPRAATSLSLNLLASTLAMKEPPTHTR
                     LRRLISRDFTMREIDNLRPSIARFVAARLDGMAPALERGEAVDLHRQFALALPMLVFAE
                     LFGMPQDDMFGLAAGIGAILEGLSPHASDPQLAAADAASARMKAYFGDLIQRKCIDPRH
                     DIVATLVGAHDDDADTLSDAELISMLWGMLLGGFATTAATIDHAVLAMLAYPDQRHWLQ
                     GDAAGVEAFVEEVLRCDAPAMFSSIPRIAQSDIELSGVVIPKNADVRVLIAAGNRDPDA
                     FADPDRFDPARFYGTSPGMSTDGKIMLSFGHGIHFCLGAQLARVQLAESLPRIQARFPT
                     LTVAEQPTREPSAFLRTFRALPVRLHAQGDSPRLTSAFLNGQRGVEGGASFEHGDGERR
                     SATDRRAQP"
     misc_feature    2592..2594
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2729..3037
                     /locus_tag="NGR_RS31345"
                     /old_locus_tag="NGR234_211"
                     /old_locus_tag="NGR_a02720"
     CDS             2729..3037
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1265:ferredoxin
                     (Score: 67.8; E-value: 1.1e-20)"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204698.1"
                     /locus_tag="NGR_RS31345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGR234_211"
                     /old_locus_tag="NGR_a02720"
                     /product="ferredoxin"
                     /protein_id="WP_010875297.1"
                     /transl_table=11
                     /translation="MRVVIDQDLCGTTGQCVLTLPGTFRQREPDGVAEVCVATVPQALH
                     AAVRLAASQCPVAAIRVIESDAGDDERASADPARSPAEAERHAAKDQRIPGGHDGTV"
     gene            3024..3860
                     /locus_tag="NGR_RS31350"
                     /old_locus_tag="NGR234_210"
                     /old_locus_tag="NGR_a02730"
     CDS             3024..3860
                     /NRPS_PKS="Domain: PKS_KR (7-176). E-value: 1.6e-09. Score:
                     29.7. Matches aSDomain:
                     nrpspksdomains_NGR_RS31350_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     218.1; E-value: 2.2e-66)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875298.1"
                     /locus_tag="NGR_RS31350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGR234_210"
                     /old_locus_tag="NGR_a02730"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_010875298.1"
                     /sec_met_domain="adh_short (E-value: 2e-33, bitscore:
                     105.0, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERFEGKVAVVTGAGAGIGKACALAIAREGGRVVVADLDGSAAIA
                     CTAQIAAEAGNALAMAMDIADAQAVAALFETAERHFGGVDLLVNNASAMHLTPRDRAIL
                     DLDLAVWDQTMATNLRGTLLCCRQAIPRMIARGGGAIVNMSSCQGLSGDTAQTSYAVSK
                     AAMNMLSASLATQYGHAQIRCNAVAPGLIMTERLLAKLDKCMQRHLSRHQLLPRVGHPE
                     DVAALVAFLLSDDASFITGQVVCIDGGMLAHMPTYADGGNSCAGRTAGDTAETARC"
     aSDomain        3045..3551
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_NGR_RS31350_PKS_KR.1"
                     /evalue="1.60E-09"
                     /label="NGR_RS31350_PKS_KR.1"
                     /locus_tag="NGR_RS31350"
                     /protein_end="176"
                     /protein_start="7"
                     /score="29.7"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAVVTGAGAGIGKACALAIAREGGRVVVADLDGSAAIACTAQIAA
                     EAGNALAMAMDIADAQAVAALFETAERHFGGVDLLVNNASAMHLTPRDRAILDLDLAVW
                     DQTMATNLRGTLLCCRQAIPRMIARGGGAIVNMSSCQGLSGDTAQTSYAVSKAAMNMLS
                     ASLATQ"
     gene            3860..5203
                     /locus_tag="NGR_RS31355"
                     /old_locus_tag="NGR234_209"
                     /old_locus_tag="NGR_a02740"
     CDS             3860..5203
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 200.4; E-value: 8.5e-61)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838317.1"
                     /locus_tag="NGR_RS31355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGR234_209"
                     /old_locus_tag="NGR_a02740"
                     /product="cytochrome P450"
                     /protein_id="WP_010875299.1"
                     /sec_met_domain="p450 (E-value: 3.2e-44, bitscore: 140.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVLLNPLNRRHRLRYDIPVMPGAFPLVGHLPAIVCDLPRLLRRA
                     ERTLGSHFWLDFGPAGHLMTCVDPHAFALLRHKDVSSALIEEIAPELLGGTLVAQDGGA
                     HRQARDAIKAAFLPEGLTQAGIGDLFAPVIRARVQAWRDRGDVTILPETGDLMLKLIFT
                     LMGVPAQDLPGWHRKYRQLLQLIVAPSVDLPGLPLRRGRAARDWIDAQLRQFVRDARAH
                     AARTGLINDMVSAFDRSDDALSDDLLVANIRLLLLAGHDTTASTMAWMVIELARQPMLW
                     DALVEEAQRVGAVPTRHADLEQCPVAEALFRETLRVHPATTLLPRRALQELQLGQRRIP
                     AGTHLCIPLLHFSTSALLHEAPDQFRLARWLQRTEPIRPVDMLQFGTGPHVCIGYHLVW
                     LELVQFSIALALTMHKAGVRPLLLSGVEKGRRYYPTAHPSMTIRIGFS"
     gene            5357..6355
                     /locus_tag="NGR_RS31360"
                     /old_locus_tag="NGR234_208"
                     /old_locus_tag="NGR_a02750"
     CDS             5357..6355
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182:Polyprenyl synthetase (Score: 183.9; E-value:
                     5.8e-56)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605890.1"
                     /locus_tag="NGR_RS31360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGR234_208"
                     /old_locus_tag="NGR_a02750"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_010875300.1"
                     /transl_table=11
                     /translation="MQTGSTLHDDRTSVSALGGLGARARGASGRLLPEIWMQNGAKRVE
                     QALARLLCAEDHGETELMAAMRYATLHGGKRTRALLCLAAGALADTPAHILDDVGAAIE
                     MMHACTLVHDDLPAMDDDVLRRGLPTVHVKFGEATAILVGDALQAHAFLTLASLDAPGD
                     NRIALVRELAQAVSAEGAAGGQAMDLSLVGKHVELDRIVAMHRMKCGALVRASVRMGAL
                     CAIAEDAADATLYCALDHYSACFGLALQVVDDILDATADTATLGKTPGKDAAAQKPTCA
                     SIMGLQAARQFALDLLCEAGEAIAPLGPRAERLAQMLQRASAYLFKHAPSA"
     gene            6666..8216
                     /locus_tag="NGR_RS31365"
                     /old_locus_tag="NGR234_207"
                     /old_locus_tag="NGR_a02760"
     CDS             6666..8216
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026312707.1"
                     /locus_tag="NGR_RS31365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGR234_207"
                     /old_locus_tag="NGR_a02760"
                     /product="hypothetical protein"
                     /protein_id="WP_010875301.1"
                     /transl_table=11
                     /translation="MNALSEQILFELRHLLSEMSDGGSVGPSVYDTARALQFGGNVTGR
                     QDAYAWLLAQQQADGGWGSADFPLFRHAPTWAALLALQRADPLPGAADAVQAATRFLER
                     QADPYAHAVPEDAPIGAELILPQLCGEAASLLGGVAFPRHPALLPLRQACLVKLGAVAT
                     LPSGHPLLHSWEAWGTWPTAACPDDDGSIGISPAATAAWRAHAVTQGSTPQVGRADAYL
                     QAASRATRSGIEGVVPNVWPINVFEPCWSLYTLHLAGLFAHPALDEAVRVIVAQLDARL
                     GVRGLGPALHFAADADDTAVALCVLRLAGRDPAVDALRHFEIGELFVTFPGERNASVST
                     NIHALHALRLLGKPAAGTSAYVEANRNPHGLWDNEKWHVSWLYPTAHAVAALAQGKPQW
                     RDERALAALLQAQRDDGGWGAGRASTFEETAYALFALHVMDGSEEPTGRRRIAQAVARA
                     LEWMLARHAAPALPQMPLWIGKELYCPIRVVRVAELAGLWLALRWGPRVPAEGAGAAP"
     gene            8213..9118
                     /locus_tag="NGR_RS31370"
                     /old_locus_tag="NGR234_206"
                     /old_locus_tag="NGR_a02770"
     CDS             8213..9118
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084944.1"
                     /locus_tag="NGR_RS31370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NGR234_206"
                     /old_locus_tag="NGR_a02770"
                     /product="hypothetical protein"
                     /protein_id="WP_010875302.1"
                     /transl_table=11
                     /translation="MIPTERGLQQVLEWGRSLTGFADEHAAEAVRGGQYILQRIHPSLR
                     DTSARTGRDPQDETLIVAFYRELALLFWLDDCNDLDLIAPEQLAAVEQALGQGVPCALP
                     GFEGCAVLRASLAALAYDRRDYAQLLDDTRCYCAALRAGHAQAAGAAERWSYAEYLHNG
                     IDSIAYANVFCCLSLLWGLDMATLRARPAFRQVLRLISAIGRLQNDLHGRDKDRSAGEA
                     DNAAILLLERYPAMPVVEFLNDELAGHTRMLHRVMAEERFPAPWGPLIEAMAAIRAHYY
                     QTSTSRYRSDAAGGGQHAPA"
ORIGIN
        1 atgcccgaac aacccttgcc gacgctgccg atgtggcggg ttgatcacat cgagccctcg
       61 cccacgatgt tggcgctacg cgccaacggt ccgattcaca acgtgcgctt cccgcggggg
      121 cacgaaggct ggtgggtgac aggctacgac gaggcgaagg cggtgctgtc cgacgcggcg
      181 ttccggcctg cgggaatgcc gccggcggca ttcaccccgg attgtgtgat tctcggttcg
      241 ccggggtggc tggtctcgca cgaagggggc gagcatgccc ggttacgcac gatcgttgcg
      301 ccggccttca gcgaccgcag ggtgaagctg ctcgcgcagc aagtcgaggc gatcgcagcg
      361 cagttgttcg agactctggc ggcccagccc cagcccgccg acctgcggcg ccacctctcc
      421 tttccgcttc cggccatggt catcagcgcg ctgatgggcg tgctctacga ggatcacgcc
      481 ttttttgccg ggctgtccga cgaggtgatg acgcaccagc atgaaagcgg cccgcgcagc
      541 gcgtcgcgcc tggcctggga agaactgcgc gcctacattc gcggcaagat gcgggacaag
      601 cgccaggatc cgggcgacaa cctgctgacg gatctgctcg cggcggtcga ccggggcgag
      661 gcgaccgagg aagaggcgat cggcctcgcg gcgggcatgc tggtggcagg acacgagagc
      721 accgtcgcgc agatcgaatt cggcctcctg gccatgttgc gccatccgca acagcgcgaa
      781 cgtctggtcg gcaatccatc cctggtggac aaggcggtgg aggaaatcct gcgcatgtac
      841 ccgccgggcg cgggctggga cggcatcatg cgctatccga ggaccgacgt gaccatcgcg
      901 ggcgtgcata ttcccgcgga gagcaaggtg ctggtcggcc tgccggcgac atcgttcgat
      961 ccacgccatt tcgaggaccc tgaaatcttc gacatcggac gcgacgcaaa gccgcacctg
     1021 gcgttttcct acgggccgca ctactgcata ggcatggcgc tggccaggct ggaactcaag
     1081 gtggtgttcg gttcgatctt ccagcgcttt cccgcgctgc gcctggccgt ggcgcccgaa
     1141 gaactgaagt tgcgcaagga gatcatcact ggcggattcg aggagttccc ggtgctctgg
     1201 tgatgcgcgg acgccgccgg gaatcgcgat cttctccgca atttgcaggc gcgcctggcg
     1261 cgcctgtcag atcagccagc cgacaggtaa ccaagatgga catgcaagaa accacgacag
     1321 catgcgcgga cgcctttgcc gaactggcgt cgccagcctg catcgacgac ccgtatccgt
     1381 tcatgcgctg gttgcgcgag cacgatccgg tgcatcgcgc ggcgtcgggc ctctttctgt
     1441 tgagccgcca cgccgacatc tgctgggcgc tcaaggccac gggcgacgcg tttcggggac
     1501 cggcgccggg cgaactggcg cgctatttcc cgcgtgcggc gaccagcctg tcgctcaatc
     1561 tgctggcgtc cacgctagcg atgaaggaac caccgacgca tacgcgtctg cgccggctga
     1621 tctcgcgcga tttcaccatg cgcgagatcg acaacctgcg gccgagcatc gcgcgcttcg
     1681 tcgcagcgcg cctggacggc atggcgcccg cgctggagcg cggggaggcg gtggacctgc
     1741 atcggcaatt cgcgctggcc ttgcccatgc tggtcttcgc cgaactgttc ggcatgccac
     1801 aggacgacat gttcggcctc gccgccggca tcggcgccat tctggaaggc ctgagcccgc
     1861 acgccagcga tccccagctc gccgcggcag acgcggccag cgccaggatg aaggcctact
     1921 tcggcgacct gatacagcgc aagtgcatcg atccccgcca cgacattgtg gccacgctgg
     1981 tcggcgcaca cgacgacgat gccgacacgc tatcggatgc ggagttgatc agcatgctgt
     2041 ggggcatgct gctgggcggc ttcgccacca ctgctgcgac catcgaccat gccgtcctgg
     2101 cgatgctggc gtatcccgac cagcggcact ggctgcaggg agacgccgcg ggggtggagg
     2161 cattcgtcga agaggtcctg cgctgcgacg cccccgccat gttcagctcc attccgcgta
     2221 ttgcccagag cgacatcgaa ctgagcggcg tggtgatccc gaagaacgcg gacgtgcgcg
     2281 tgctgatcgc ggccggcaat cgcgacccgg acgccttcgc cgatcccgac cgcttcgatc
     2341 ccgctcggtt ctacggcacc agtcctggca tgtcgaccga cgggaagatc atgcttagct
     2401 tcggccacgg catccacttc tgcctcggtg cgcaactggc ccgggtgcag ttggccgaga
     2461 gcctgccgcg aatccaggcg cgcttcccca cgctgacagt ggccgagcag ccgacccggg
     2521 agccctccgc gttccttagg acgttccgcg cgctgccggt gcggctgcat gctcaggggg
     2581 actccccccg tttaacaagc gcatttttga acggccagag aggggtcgaa ggtggtgcca
     2641 gttttgagca tggcgacggc gagaggcggt cggcgactga tcgcagggcg caaccgtaga
     2701 accgcatgaa aagggtacaa gggctgagat gcgcgtcgtg attgaccaag atctgtgcgg
     2761 aaccactggg cagtgcgtgc tgacgctgcc gggcaccttt cgccagcgcg aaccggacgg
     2821 cgtggccgaa gtgtgcgtgg cgacggtacc gcaggcgctg cacgccgccg tgcggctcgc
     2881 ggccagccag tgcccggtcg ccgccattcg ggtcatcgaa agcgacgctg gcgatgacga
     2941 gcgcgccagc gccgaccctg cgcgttctcc ggcggaggcc gagcggcatg cggcgaaaga
     3001 ccaacgcatt ccaggaggac acgatggaac ggtttgaagg caaggtggcc gtggtgaccg
     3061 gcgccggcgc cggcatcggc aaggcatgtg ccctcgccat cgcgcgcgag ggcggcagag
     3121 tggtggtggc cgaccttgat ggctcggcgg ccatcgcctg caccgcgcag atcgcggccg
     3181 aagcgggcaa tgcgctggcc atggccatgg acatcgccga tgcgcaggca gtggcagcgc
     3241 tgttcgagac ggcggagcgg cacttcggtg gggtcgacct gctggtgaac aacgcgagcg
     3301 ccatgcatct gaccccgcgc gaccgcgcga tcctcgacct ggacctggcg gtctgggatc
     3361 agaccatggc gaccaatctg cgcggcacgc tgctctgctg ccgacaggcc atcccacgga
     3421 tgatcgcccg cggcggtggc gcgatcgtca acatgtcgtc gtgccagggg ctcagcggtg
     3481 acaccgcgca gacgtcatac gccgtgtcga aggcggcgat gaacatgctg tcggcctcgc
     3541 tcgccaccca gtacggccat gcgcagatcc gctgcaacgc ggttgcgccg ggtctcatca
     3601 tgaccgagcg tctcctggcc aagctggaca agtgcatgca acggcatctc agccggcacc
     3661 agctcctgcc gcgcgtcggc caccccgagg acgtggccgc gctggtggcg ttcctgctct
     3721 ccgacgatgc ttcgttcatc accggccagg tcgtgtgcat cgacggcggc atgctggcgc
     3781 atatgccgac gtacgccgac ggtggcaaca gctgcgccgg gcggactgcg ggcgacaccg
     3841 ccgaaacggc gcgctgctga tgaacgtgct gctcaacccc ctaaaccgtc ggcaccggct
     3901 gcggtacgac atccccgtca tgcccggcgc tttccccctg gtcgggcatc ttcccgccat
     3961 cgtctgcgac ctgccgcgcc tgctgcggcg cgcggaacgg acgctgggca gccacttctg
     4021 gctggatttc ggccctgccg gacacctgat gacctgcgtg gatccgcatg cgttcgcact
     4081 gctccggcac aaggacgtgt cctcggcgct gatcgaagag atcgcgcccg aattgcttgg
     4141 cggaacgttg gtcgcccagg acggcggcgc gcaccggcag gcgcgcgatg cgattaaggc
     4201 ggcgttcctg cccgaggggc tgacccaggc cggcatcggc gacctgttcg cgcccgttat
     4261 ccgggcgcgg gtgcaggcgt ggcgcgaccg cggcgacgta accatcctgc ccgaaaccgg
     4321 cgacctgatg ctcaagctca tcttcaccct catgggagtc cctgcgcagg acctgccggg
     4381 atggcatcgc aagtaccggc aactactgca gttgatcgtc gcgccctcgg tcgacctgcc
     4441 tggactgccc ttgcggcgcg gccgcgccgc ccgcgactgg atcgacgcgc agttgcgcca
     4501 gttcgtccgc gacgcacgcg cgcatgccgc gcgcaccggg ttgatcaacg acatggtgag
     4561 tgccttcgac cgcagcgacg atgcgctctc cgatgacctc ctggtcgcca atatccgctt
     4621 gctgctgctt gccggtcacg acaccaccgc ctcgacgatg gcctggatgg tgatcgaact
     4681 ggcgcggcag cctatgctgt gggatgccct ggtcgaggag gcacaacgcg taggcgcggt
     4741 gccgacccgg cacgcggacc tggagcagtg tccggtcgcc gaggcgctgt tccgcgagac
     4801 gctgcgcgtg catccggcga cgacgctcct gccgcgtcgc gcgctgcagg aactgcaact
     4861 cggccaacgg cgcattccgg cgggcaccca tctgtgcatc ccgctgctgc atttctcgac
     4921 ctcggcgctg ctgcacgagg cgcctgatca gttccgcctg gcgcggtggc tgcaacgtac
     4981 ggagccgatt cggccggtgg acatgctgca gttcggtacc ggcccacacg tttgcatcgg
     5041 ctatcacctg gtatggctgg aactggtgca gttctccatc gccttggcgc tgaccatgca
     5101 caaggccggg gtgcggccgc tgttgctgag cggcgtcgaa aaaggccggc gctattaccc
     5161 gaccgcacat ccgtccatga caatccgcat cggattctca tgagctggca tcgcatgccc
     5221 cgtgtggcga ggcagcggca ccggcgacgc gcggcattgc tgcattcggc gcgcgcgttc
     5281 ggtgcgacgc ggcaacaccg cggcggctcg acgctcgccg tggccgtctc ctgtcaggta
     5341 caaggacatg aacaacatgc agaccggttc cacgctacac gacgaccgaa ctagcgtttc
     5401 cgccctcggc ggattgggcg cgcgggcgcg cggcgcatcc ggcaggctgc tgccggagat
     5461 ctggatgcag aacggcgcaa agcgggtcga acaggcgcta gcgcgtcttc tctgcgccga
     5521 agaccacggt gagaccgagc ttatggcggc gatgcgctac gccaccttgc atggcgggaa
     5581 gcgcacccgc gccctgctct gtctggctgc tggagcactg gccgacacgc cggcgcacat
     5641 actcgacgac gtcggcgccg ccatcgagat gatgcacgct tgtaccctgg tccacgacga
     5701 cctgcccgca atggacgacg acgtgcttcg ccgcggcctt ccgaccgtgc atgtcaagtt
     5761 cggcgaagcc actgcgatcc tggtcggcga tgcgctgcag gcacacgcct tcctgaccct
     5821 ggcaagcctg gatgcgccgg gcgacaaccg tatcgcgctc gtgcgcgaac tggcgcaggc
     5881 ggtgtctgcc gagggtgccg caggcgggca ggccatggat ctgtcgctgg tcggaaagca
     5941 cgtcgagctg gacaggatcg tagcgatgca ccggatgaag tgcggagcgc tagtgcgtgc
     6001 gtccgttcgc atgggcgccc tatgcgccat cgcggaggat gccgcggacg ctacgctgta
     6061 ctgtgcgctc gatcactaca gcgcctgttt cggcctggcg ttgcaggtgg tcgacgacat
     6121 tctcgacgcg acagcggata ccgcgacgct gggcaagacc cccggcaagg acgcggcggc
     6181 gcagaaaccg acctgcgcgt cgatcatggg gctgcaggca gcgcgccagt tcgcgctgga
     6241 tctgttgtgt gaggccgggg aggccattgc cccgctgggg ccgcgtgcgg aacggttggc
     6301 gcagatgctg cagcgggcca gtgcgtacct gttcaagcac gcgccatccg catgagcgcg
     6361 cccgtccgca tggagtcgcc cctgtgccgc tgcgatcggc cggcggcagc catgcatgca
     6421 gcactgtgtc cgagtccgcg gcgccgatcg ccgcggttgc ccagcacggt cacggcgcac
     6481 gcggcacgct gcgcgcaagc ccatgcggcg gagaaccggc gcccgcatcg gggctcatcg
     6541 ccgcgccgga gcagagcggc cgtccggcgc tgcccatgcg gcgggccgca acgggcctgc
     6601 ggcgacgtgc gcgccgtctg cccgatcctg gttctcgtca accgtctgca gaaggaacat
     6661 cccccgtgaa tgcgctgtcc gaacagatcc ttttcgaatt gcgccacctg ctgagcgaga
     6721 tgagcgacgg cggcagcgtc ggtccgtccg tctacgacac ggcgcgggct ctgcagtttg
     6781 gcggcaacgt caccggtcgg caggacgcat acgcgtggct cctcgcgcag caacaggccg
     6841 atggcggatg gggaagcgcg gacttcccgc tgttccgcca tgcgcccacg tgggcggcgt
     6901 tgctggcatt gcagcgtgcc gatcctcttc ccggcgctgc cgacgcagtc caggctgcaa
     6961 cccggttcct cgagcgccag gccgatccct acgcgcatgc ggtgccggaa gacgcgccga
     7021 tcggcgcgga gctgatcctg ccgcagttgt gcggcgaggc cgcatccttg ttgggcggcg
     7081 tggcgtttcc gcgccacccc gcgctgttgc cgttgcggca agcgtgcttg gtcaagctgg
     7141 gggcggtggc gacgttgccg agcggccatc cgttgctgca ctcctgggag gcctggggga
     7201 cgtggccaac cgccgcatgc ccggatgacg acggcagcat cggcatcagt ccggcggcca
     7261 ccgctgcgtg gcgtgcgcac gccgtgacac aggggagcac gccgcaggtc gggcgcgccg
     7321 acgcgtatct gcaggcggca tcgcgggcga cgcgcagcgg catcgaaggt gtcgttccca
     7381 acgtctggcc gatcaatgtg ttcgagccat gctggtcgct gtacactcta catttggccg
     7441 ggctgttcgc gcatcccgcg ctcgacgagg cggtgcgcgt gatcgtcgcg cagctcgacg
     7501 cccgcctggg cgtgcgcggt ctgggcccgg ccttgcactt cgcggctgat gcggacgaca
     7561 ccgccgttgc cttgtgcgtc ctgcgccttg caggccgcga cccggcggtc gatgcgttgc
     7621 gccatttcga aatcggcgag ctgttcgtca ccttccccgg cgagcgcaac gcctcggtgt
     7681 cgaccaacat ccatgccctg catgcgttgc gactattggg aaagcctgcc gctggcacca
     7741 gcgcctacgt cgaggccaat cgcaaccctc acggtctatg ggacaatgaa aaatggcacg
     7801 tttcgtggct gtatcccacc gcgcatgccg tcgctgcgct ggcgcaaggc aagccccagt
     7861 ggcgcgacga gcgcgcgctg gcggcgctgc tgcaggcgca gcgcgacgac ggcggctggg
     7921 gcgccggtcg cgcgtccaca ttcgaggaaa ccgcctatgc gctgttcgcg ttgcacgtga
     7981 tggacgggag cgaagagccg acagggcgcc ggcgcatcgc gcaggcggtg gcgcgtgcgc
     8041 tggagtggat gctcgcccgc catgcggcgc ctgcattgcc gcagatgccg ctgtggatcg
     8101 gcaaggaact gtattgcccc atccgggtcg tgcgcgtggc cgaactcgcc gggttgtggc
     8161 tggcgcttcg ttgggggccg cgcgtcccgg ccgagggagc aggagcggcg ccatgatccc
     8221 gaccgaacgc gggctgcagc aggtgctgga gtgggggcgt tccctgaccg ggttcgccga
     8281 cgagcatgcc gcggaagccg tcaggggcgg ccagtacatc ctgcagcgca tccacccgag
     8341 cctgcgcgac accagcgccc gcaccggtcg cgatccgcag gacgaaacgc tgatcgtggc
     8401 attctatcgc gaactggcgc tgctgttctg gctcgacgat tgcaacgacc ttgacctgat
     8461 cgcgccggag cagctcgccg cggtggagca ggcgctgggg cagggcgtgc cgtgcgcgct
     8521 ccccggattc gagggctgcg ctgtgctgcg cgcttcgctg gccgccctcg cctacgatcg
     8581 tcgcgactat gctcagcttc tcgacgatac ccggtgctac tgcgcggcgc tgcgcgccgg
     8641 acacgcgcag gcggcagggg cggcggaacg ctggtcctac gccgagtacc tgcacaacgg
     8701 catcgattcg atcgcctacg caaacgtgtt ctgttgcctg tcgttgctgt gggggctgga
     8761 catggcgacc ttgcgcgcgc gtccggcgtt tcgccaggtc ctgcggctca tctcggcgat
     8821 agggcgcctg cagaacgatc tgcatggacg cgacaaggac aggtcggccg gcgaggccga
     8881 caacgcggcg atcctgctgc tggagcgcta tccggctatg cctgtggtgg agttcctcaa
     8941 cgacgaactg gccggccata cgcgcatgct gcaccgggtt atggcggaag aacggtttcc
     9001 cgcgccgtgg gggccgttga tcgaggccat ggcggccatc cgcgcgcact attaccagac
     9061 ctcgaccagc cgctaccgca gcgacgctgc ggggggaggc cagcatgcgc ccgcctga
//
