LOCUS       BGC0001618              8595 bp    DNA     linear   BCT 05-APR-2017
DEFINITION  Staphylococcus hyicus plasmid pRJ109 hyicin 3682 gene cluster,
            complete sequence.
ACCESSION   BGC0001618
VERSION     BGC0001618
KEYWORDS    .
SOURCE      Staphylococcus hyicus
  ORGANISM  Staphylococcus hyicus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 02:54:34
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /collection_date="24-Mar-1999"
                     /country="Brazil"
                     /db_xref="taxon:1284"
                     /identified_by="Patricia Carlin Fagundes"
                     /isolation_source="milk from bovine mastitis"
                     /mol_type="genomic DNA"
                     /organism="Staphylococcus hyicus"
                     /plasmid="pRJ109"
                     /strain="3682"
     protocluster    1..8595
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[270:4501]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      271..4501
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..8595
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8595
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001618"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8595
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            71..214
                     /gene="hyiA"
     CDS             71..214
                     /codon_start=1
                     /gene="hyiA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gallidermin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_b"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="lantibiotic precursor peptide; structural protein"
                     /product="HyiA"
                     /protein_id="ARD24445.1"
                     /sec_met_domain="Gallidermin (E-value: 8.7e-26, bitscore:
                     79.3, seeds: 3, tool: rule-based-clusters)"
                     /sec_met_domain="mature_b (E-value: 5.4e-15, bitscore:
                     44.1, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKILDLDVQVKAQKESNDSSGDERITSFSLCTPGCAKTGSFNSY
                     CC"
     CDS_motif       71..139
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MEKILDLDVQVKAQKESNDSSGD"
                     /locus_tag="hyiA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       140..214
                     /prepeptide="core"
                     /locus_tag="hyiA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="2.20"
                     /molecular_weight="2403.8"
                     /monoisotopic_mass="2402.1"
                     /core_sequence="ERITSFSLCTPGCAKTGSFNSYCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEKILDLDVQVKAQKESNDSSGD"
                     /leader_location="[70:139](+)"
                     /alternative_weights="2421.8"
                     /alternative_weights="2439.8"
                     /alternative_weights="2457.8"
     misc_feature    71..8595
                     /note="hyicin 3682 gene cluster"
     gene            271..3264
                     /gene="hyiB"
     CDS             271..3264
                     /codon_start=1
                     /gene="hyiB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     827.4; E-value: 2.4e-250)"
                     /gene_kind="biosynthetic"
                     /note="desidratase; amino acid modification"
                     /product="HyiB"
                     /protein_id="ARD24446.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.8e-132,
                     bitscore: 431.6, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.1e-17, bitscore:
                     53.5, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 4e-14,
                     bitscore: 42.3, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVIYEPSKICMIRTPLLSLNYFYNYLNKENINYEYLVSDSLVKE
                     SILTTTVNLYNSLHNVYSNSKNKKNRDTKESLLKYLIRMSIRPTPFGLLSGINIGEFKK
                     ENTQIKLGNNAQKYVKIDGEWLYKLISYIESTYSLYKDLKVIWNDCCHIVNDRIYLYEQ
                     TAVYLNNNESGSFSIKNSCILSYIKYTVENKDISFARLVEEISVEFDIKDQEQIKKYIH
                     TLITKEVIYSDVRPPLSNENNFQYILDKLCFCNEEFKKKLESINSSIIAYEECEIGDGS
                     ELYLNIIKDMEALFPCRNYLQIDTKVDFVTNVLNENISKRISEAAYLLWLLSKNNIGSR
                     NLKNFQKQFLEHYGFEQLVNVKDLISEIKGFGSLIYTEGEDQGNNIDFLKQKYLKALKT
                     NGEVEINDIDFQKLIKENKVNEYHAPISSDIYSELYIGKFFNSYSELIVISPITTSSNA
                     GATFGRFHHLIDLKYLLELEREKKYYYEKFMENDDIEMVSINELPKYPRHQNVLFNNDS
                     FDYIVNLGTNCSKRGKDLSLDELYVGATINKLYLYSSSLNKRVLFESNNMFNFFKSSNL
                     YRFLKEVSMESVKIIEPLNEFSIDAFSYLPRIRYKNVVLRLASWKINSSVLALPQSDEW
                     DNLFLEYKKEFDIPNMVNLIYGDNKLLLNLELSSHRYLLMKEYKKNKRVRLVETFVPNS
                     SNEYTYEIVTPIYKKRNFSGPKIEIPKNKNKDIQYDRNWLSIHIYINREFQDEFIVQYL
                     NPLMEKNKYNKLLDSYFIIRYIDDKDFLKLRIYRYNEDYKELFDNLNKWFTNVKMYTEV
                     SSFEFVEFIPEYYRYGGENAIYAIEEFFDYDTGVAVNIIRDQFEFEKEYITAISIIYLF
                     EKANITNYEREDIVDNYVSTSYRTKEIRIIKNELVRMCNPKNNFEYLRNEYKDIFSAFD
                     DKNNVLTLLEGSLSKPLTAKRERIVGSLIHMRCNRIFGINKEKEKFVLSIVKEIIKTQK
                     HWSGEKND"
     gene            3257..4501
                     /gene="hyiC"
     CDS             3257..4501
                     /codon_start=1
                     /gene="hyiC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     330.2; E-value: 3e-100)"
                     /gene_kind="biosynthetic"
                     /note="cyclase; amino acid modification"
                     /product="HyiC"
                     /protein_id="ARD24447.1"
                     /sec_met_domain="LANC_like (E-value: 4.1e-73, bitscore:
                     235.2, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDINEILERKFKEISDVENFLNFASSKTEFWEPGTLSHGIPGII
                     LYLESYQKAFNTNTEQISYKYIVELSKSLSNGTFNESLFSGLSGMGFSIDLASQDGKNY
                     QNLLKKIDAVIIENIYVKIDNLRKKGLNPIDYDVISGLSGIGRYLLNRVQYNSNVTLHL
                     KNILKYFRDIHYSYEGWTISRENQFLEKDKEYFKKGNINLGLAHGILGPISLFALNYIQ
                     GITIENHRQTLKDMAAYIVENRFCKDLRWLYRYNFIKEQNNINYIRNGWCYGNTAIMRT
                     LFLISIALDDSYIKGLSKDIMYQVLNDGFVNLISPTICHGLSSHLIMLKLMNLYFKDPN
                     VDDYLEKVEEKILSHYTENDNLNFLDINEKNNSKIKKSKLGILEGELGVLITLMTHKNP
                     SLLKGKNWTNAFLIS"
     gene            4518..5048
                     /gene="hyiD"
     CDS             4518..5048
                     /codon_start=1
                     /gene="hyiD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Flavoprotein"
                     /gene_kind="biosynthetic-additional"
                     /note="cystein-decarboxylase; amino acid decarboxylation"
                     /product="HyiD"
                     /protein_id="ARD24448.1"
                     /sec_met_domain="Flavoprotein (E-value: 3.4e-32, bitscore:
                     100.2, seeds: 143, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRNVLLCLCGSINSINITHYIIELKSKYDEVNILASGNGKKFVN
                     ANILKQFCDNYYDEAENPFLNHIDISKKHEKVLIIPATANTINKIANGICDNLLLTICH
                     TSFDKLYIFPNMNLLMWENPITKKNIDKLKNYGISIYPSNIRNSFEISSRTMKKNVVMP
                     DPHEVLDFILKRR"
     gene            5078..6430
                     /gene="hyiP"
     CDS             5078..6430
                     /codon_start=1
                     /gene="hyiP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 257.9; E-value: 3.7e-78)"
                     /gene_kind="biosynthetic-additional"
                     /note="protease; proteolytic processing"
                     /product="HyiP"
                     /protein_id="ARD24449.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.2e-43, bitscore:
                     138.5, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVSLLIPNKLTLAQDYQYYSVEYIDTLEFEKLIKNESVEVVYRI
                     PKLKIAQIKTKSKTAKKLSLHRKAIRYINLTCSTCNNNEKTTSIYPPISSTESLFSRQW
                     DMNRVTKNGMIYENLPKNNKTQIAIIDTGITMNHEDLKYNHSNKSKNLVPVNGFRGTES
                     DEKGTIDYLIDKKGHGTMVAGQSSANGRLVGVAPKNKFNMYRVFGSKKTEMLWVANAIV
                     TAADDGNQVINISVGSYIILDKKQKAVFRNDEKVEYNALQKAINYAKKKKSLVVAANGN
                     NGIDVNNIKQINSQRNYNKDGDGRVYDVPASLENVVTVGSTDQNGNLSEFSNYGKHYTD
                     IVAPGGSYQYLNKYGHDKWIDDGYMHKENILTTANNGRYIYQAGTSLAAPKVSGALALI
                     IDYYSLEENPQKAITILYDNGTSSVNKPYEKYGHGELDIYQLIKKPIKKAS"
     gene            6453..7142
                     /gene="hyiF"
     CDS             6453..7142
                     /codon_start=1
                     /gene="hyiF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 156.6; E-value:
                     1.4e-47)"
                     /gene_kind="transport"
                     /note="ATP-binding protein; ABC transporter"
                     /product="HyiF"
                     /protein_id="ARD24450.1"
                     /transl_table=11
                     /translation="MTKLVTRDISKKFKSQHILNKINISLQNNEVYGLLGINGAGKTTL
                     IKIICGIIQQDSGEIFLDDRPMVRDDLHKVGSLIETPATYNHLSAQDNLKIVCLNENIS
                     FEDIYEVLKLVNLDVDSKKKVKDFSLGMKQRLGIAMALIKKPDILILDEPSNGLDPYGI
                     QELRELLKSITQKGTSVIISSHILGEIQILADHIGIIHDGELKYQQINNKSENLEEIFF
                     NITKGDR"
     gene            7142..7900
                     /gene="hyiE"
     CDS             7142..7900
                     /codon_start=1
                     /gene="hyiE"
                     /note="membrane protein; ABC transporter"
                     /product="HyiE"
                     /protein_id="ARD24451.1"
                     /transl_table=11
                     /translation="MKYLKAEALKFKNSFSMYVLFLSPLVFLCFSIFTAVFAKSNSLTP
                     NGLSPYITLLFNLWPIAFIPIVVCLACYSLIMIETRNKMFNFYLSNNWDLTKEIKAKIF
                     IISFAFLIHGLMMYIVALVGDFLISPSPIKPSLLLATILIIFWVAIPLIPLNFLLLRYL
                     GIFLTIFVNLVLSIFSIIFLTLKSVFWLSPWAIMQKIPLITLGILPNGLTVDKYSKYFN
                     DLESLYISIIVSIVTYIIFTILNTKKSWRL"
     gene            7900..8595
                     /gene="hyiG"
     CDS             7900..8595
                     /codon_start=1
                     /gene="hyiG"
                     /note="membrane protein; ABC transporter"
                     /product="HyiG"
                     /protein_id="ARD24452.1"
                     /transl_table=11
                     /translation="MINELRVCNLKFSNQFLTYLPILISFIFVFFINFYINSKTWDDDQ
                     ISLYTSSFNVMVSIFLSINVYQVINFEENIGHFNHILSKPKRINWALSVLFFSYSITSL
                     CIIIAVINLFIHTNNVFITLFFFVSSFVFNIITLIILFIFGVFFKSILSIIIGILNFIF
                     NVYFGLEVLGDHSWFYMPTTYSTRYIHMYIENNIPMTLTIIMYIVSTIILFCFIYIRFN
                     KWNGRVIKD"
ORIGIN
        1 ttgcaattca tattaatatg ttaatagaag tatattttta cttcatgata attataggag
       61 gttttttgct atggaaaaaa ttcttgattt agatgtacaa gtcaaagcgc aaaaagaatc
      121 aaatgattca tctggagatg aacgtattac aagtttctca ttgtgtactc ctggttgcgc
      181 taaaactggt agcttcaata gttattgttg ttaaacttat gaagatagag cctatatagg
      241 ctctatctat tataattgag gtgttaaaat atggatgtca tatatgaacc atctaaaatt
      301 tgtatgataa gaaccccgtt attatcattg aattattttt ataattactt aaacaaagaa
      361 aatattaatt atgaatattt agtatctgac tctttagtga aagaatcaat attaactaca
      421 acagtaaatt tatataattc tttacataat gtgtattcaa attctaaaaa caaaaaaaac
      481 agagatacaa aagaaagctt attaaagtat ttaattcgta tgtctattag accaactcca
      541 tttggtctat taagtggtat aaatataggt gaatttaaga aagaaaacac tcaaattaag
      601 ttggggaata atgcccaaaa atacgtgaaa attgatggcg aatggttata taaacttatc
      661 agctatattg aatctacata tagtctttat aaagacttaa aagtcatttg gaatgattgt
      721 tgtcacatag tgaatgatcg tatctatcta tatgaacaaa ctgcagtcta tctaaacaat
      781 aatgaaagtg gttctttttc aattaaaaat agctgtattt tatcctatat aaaatatact
      841 gttgaaaaca aagatattag ttttgcgaga ctagtcgagg aaattagcgt tgaattcgat
      901 attaaagatc aagaacaaat aaaaaaatat attcatacgc taataactaa agaggttatt
      961 tattcggacg ttaggccccc gttgtctaat gaaaataact ttcaatatat attagataaa
     1021 ttatgttttt gcaacgaaga atttaagaag aagcttgaaa gtataaatag ttcaataatt
     1081 gcatatgaag aatgtgaaat tggagatgga agtgagttat atttaaatat aataaaagac
     1141 atggaagcgc tattcccgtg tagaaactat ctacagattg acactaaagt agattttgtc
     1201 acaaatgtat taaatgaaaa catttccaaa cgaatcagtg aagcggctta tttgttatgg
     1261 ttattgtcta aaaataatat aggaagccgt aatcttaaaa attttcaaaa gcagttttta
     1321 gaacattatg gatttgaaca gttggtaaat gtaaaagatt tgatttcaga aattaaagga
     1381 tttggaagtt taatttatac agaaggtgaa gaccagggta ataatataga ttttttaaaa
     1441 caaaaatatt taaaagcttt aaaaactaat ggtgaagttg aaataaatga tatagacttt
     1501 caaaaattaa ttaaagaaaa taaagtaaat gaatatcatg ctcctataag ttctgatata
     1561 tattcagaac tttatatagg aaagtttttt aatagttata gtgagttaat agttatcagt
     1621 cctataacaa cttcatctaa tgctggagca acttttggaa gatttcatca tcttatcgac
     1681 ttaaaatact tattagagct tgaaagagag aaaaaatatt attatgaaaa atttatggaa
     1741 aatgatgata tagaaatggt ctctattaat gaattgccta agtatccaag acatcaaaac
     1801 gttttgttta ataatgattc ttttgactat attgttaatt tagggacaaa ttgctcgaaa
     1861 cgaggaaagg atcttagttt ggatgaactt tacgtaggtg caacaataaa caaattatac
     1921 ttatactcta gttcattaaa taaaagagtt ctatttgaat ctaataacat gtttaacttt
     1981 tttaaatcgt caaatttata cagatttcta aaagaagtat ctatggaatc tgtaaagatt
     2041 attgaaccac taaatgagtt tagtatagat gcgttcagtt accttcctag aattagatat
     2101 aaaaatgttg ttcttagatt agcttcgtgg aaaattaaca gttcggtatt agcattgccc
     2161 caaagtgacg aatgggataa tttattttta gaatataaaa aagaatttga tattcccaat
     2221 atggtgaatt taatatacgg agacaataaa ctgttgttaa atttagagct atcatcacac
     2281 cgctatttat taatgaaaga atacaaaaaa aataaaagag tacgtttggt tgagacattc
     2341 gtccctaatt caagtaatga atacacatac gaaatagtca caccaatata taaaaaaaga
     2401 aacttttcag gtcctaaaat agaaatacct aaaaacaaaa acaaagatat acaatatgat
     2461 agaaattggc tttctattca tatttatata aatagagagt ttcaagatga gtttatagtc
     2521 caatacttaa acccattaat ggaaaagaat aagtacaata aacttttaga tagttacttt
     2581 attattagat acatagatga taaagatttt ttgaaactca gaatataccg ttataatgag
     2641 gattataaag aattgtttga taatttaaac aaatggttta ctaatgttaa aatgtacaca
     2701 gaagtaagct cttttgaatt tgttgagttt atacctgagt attatagata tggtggcgaa
     2761 aatgccatct atgcaataga ggaatttttt gattatgata caggagtggc agtgaatata
     2821 attagggatc aatttgagtt tgaaaaagaa tatattactg ctatttcaat aatttatttg
     2881 tttgaaaagg ctaatattac taattatgag agggaggaca tagtagacaa ctatgtttct
     2941 acttcgtata gaactaaaga aattagaata ataaagaacg aacttgttag aatgtgtaac
     3001 cctaaaaata atttcgaata cttgaggaat gagtataagg atatatttag tgcttttgat
     3061 gataaaaata atgtattaac actgcttgaa ggttcgttat caaaaccttt aacagccaaa
     3121 agggagagga ttgtaggaag tttaattcat atgaggtgta atagaatatt tggaataaat
     3181 aaggaaaaag aaaaatttgt attgtcaatt gtaaaagaaa ttattaagac tcaaaaacat
     3241 tggtcaggtg aaaagaatga ttgatattaa tgaaattctt gaaagaaaat tcaaggagat
     3301 ttcagatgta gaaaattttc ttaactttgc gtcttctaaa acagaatttt gggaaccagg
     3361 aactttatct catggcatac ctggtatcat tctttatcta gaatcctatc aaaaggcttt
     3421 caatactaac actgaacaaa tatcatataa gtatatagtg gagctttcta aaagcttatc
     3481 taatggaacc tttaatgaat ctttgtttag cggcctgtca ggcatgggat tttcgataga
     3541 tctagcatct caagatggga agaactatca aaatttatta aaaaaaatag acgctgtaat
     3601 tatagaaaac atatatgtaa aaatagataa cctaagaaaa aaagggttaa accctataga
     3661 ttatgatgtt ataagtggat tgtccggaat tggaaggtat ttattaaata gagttcaata
     3721 taacagtaat gttactttac atttgaaaaa tattttaaag tattttagag atattcatta
     3781 ttcttacgaa ggttggacta tttcgagaga aaaccagttt ttagaaaaag ataaagaata
     3841 ctttaaaaaa ggaaatatta atctgggttt agcacatgga attttaggcc ctatatctct
     3901 gtttgcatta aactatatac aaggaattac tatagagaat catagacaaa cacttaaaga
     3961 tatggcggca tatattgttg aaaatagatt ttgtaaagat ttaaggtggt tatatcggta
     4021 taattttata aaagaacaaa acaatataaa ttatataaga aatggttggt gttacggaaa
     4081 tactgctatc atgcgaacac tatttttaat tagcatagct ttagatgaca gttatattaa
     4141 aggtttgtcg aaggatatta tgtatcaagt attaaacgat ggctttgtta atttgataag
     4201 tccaactata tgtcatggat tatcctcaca tcttataatg ctaaaattaa tgaatttgta
     4261 ttttaaggac cctaatgtag atgattatct tgaaaaagta gaagagaaga ttttatctca
     4321 ctatacagaa aacgataatt taaacttctt agatataaat gaaaaaaata acagtaaaat
     4381 taaaaagagc aaattgggta ttcttgaagg agaattagga gttttaataa ctttaatgac
     4441 tcacaaaaac ccctctttac ttaaaggtaa gaactggact aatgcatttt taatatctta
     4501 attaggaggt ttaacaaatg agtagaaatg ttttgctatg tttatgtggt tctattaata
     4561 gtatcaatat aacccactat attattgaat taaaatctaa gtatgatgaa gttaatatac
     4621 tagcctctgg caatggaaaa aaatttgtca atgctaatat actgaagcaa ttttgtgata
     4681 attattatga tgaagcagaa aatccttttt taaaccatat agatatttct aaaaagcatg
     4741 aaaaggtatt aataattcca gcgactgcta atacaataaa taaaatagcg aatggaattt
     4801 gtgacaattt attattaacc atatgccata cgtcgtttga taaactgtac attttcccga
     4861 atatgaactt actaatgtgg gagaacccaa taaccaaaaa aaatatagat aaattgaaaa
     4921 actatggtat ttcaatttat ccatctaata ttcgtaatag ctttgagata tcttcaagga
     4981 caatgaaaaa aaatgttgtt atgccagatc cacatgaagt cttagatttt attttaaaaa
     5041 gaaggtgatt cctataaaaa aaattctatt tagcataatg ttagttagtt tgttaatacc
     5101 gaataaactt acattggcac aagattatca atattatagt gttgaataca tagatacttt
     5161 agaatttgaa aagttaatca aaaatgagtc agtagaagtt gtttatagga tacctaaatt
     5221 gaaaatcgct caaattaaaa caaaatcaaa aacggcaaag aaactttcat tacacagaaa
     5281 agcaattaga tacatcaatt tgacttgttc tacttgtaat aataatgaaa agacaacgag
     5341 tatatacccc cccataagtt caactgaatc actcttttca agacaatggg atatgaatag
     5401 agtaactaaa aatggtatga tttatgaaaa tcttcctaaa aacaacaaaa cccaaatagc
     5461 tatcatagat acgggtatta ctatgaacca tgaagattta aaatataacc attcaaataa
     5521 atcaaagaat ttagttccag taaatggatt tagaggaact gaatcagatg aaaaaggaac
     5581 tattgactat ttaattgaca agaaaggcca cggcacgatg gtggctggtc aatcaagtgc
     5641 gaatggtaga ctagtaggtg ttgctcctaa gaataaattt aatatgtaca gagtctttgg
     5701 tagtaaaaag acagagatgt tatgggttgc taatgccata gtaaccgcag cagatgatgg
     5761 caatcaagtg ataaatataa gtgtcggaag ttatataatc ctagataaaa aacaaaaagc
     5821 agtttttaga aatgatgaaa aagtagaata caatgctttg caaaaagcaa ttaattatgc
     5881 taagaaaaaa aagagtcttg ttgttgccgc aaatggtaat aatggtatag atgtaaataa
     5941 tataaagcaa attaacagtc agcgtaatta taataaggat ggtgatggta gggtttatga
     6001 tgttccagca tctttggaaa acgttgttac agttggttct accgaccaaa acggaaatct
     6061 ttcggagttt tcaaactatg gtaagcacta cacagatatt gttgctccag ggggatcgta
     6121 tcagtactta aataagtatg gccatgataa atggattgat gatggataca tgcataaaga
     6181 aaacatatta actactgcaa ataatggtcg atatatttat caagcaggga catccttagc
     6241 tgctccgaaa gtctctggag ccttggcgtt aataatagat tattattctc ttgaggaaaa
     6301 tcctcaaaag gctattacta tattgtacga caatggaaca tctagtgtga ataaacctta
     6361 tgagaaatat ggacatgggg aactggacat ttatcagttg attaaaaaac ctataaaaaa
     6421 agctagttaa taactaaaag gagtttttat tcatgacaaa gttagttact cgagatattt
     6481 caaaaaaatt taaaagccaa catatcctaa ataaaatcaa tattagcctg caaaacaatg
     6541 aagtttatgg tcttttagga ataaatggtg cgggtaaaac aacattaata aaaattatat
     6601 gtggtattat tcaacaagat tcaggagaga tatttttaga tgataggcca atggtacgtg
     6661 atgatttaca taaagtcggg tcattaattg agacacctgc aacatataat cacctcagtg
     6721 cacaagataa tttgaaaata gtctgtttaa acgaaaatat ttcttttgaa gatatttatg
     6781 aagtgttaaa attagttaat ttagatgttg attctaagaa aaaagttaaa gacttttctt
     6841 tagggatgaa acaaagacta ggaattgcta tggcattaat aaaaaagcct gatatactaa
     6901 tcttagatga gccttctaat ggtttagatc catatggaat acaagaactg agagaacttt
     6961 tgaaatcaat aacacagaag gggacgagtg ttataatatc tagtcatatt ttgggggaaa
     7021 ttcaaatatt agctgatcat attgggatta tacatgatgg agaattaaaa tatcagcaga
     7081 taaataataa aagtgaaaat ttagaagaga tattcttcaa tattacgaaa ggggatcgct
     7141 aatgaagtat ttaaaagcag aagctttaaa atttaaaaac tcatttagta tgtatgtttt
     7201 atttttaagc cctttagtat ttttatgttt ttctatattt acagcagttt ttgctaaaag
     7261 taattcttta acgcccaatg gattatctcc ttatattaca ttactattta atctttggcc
     7321 tattgcattt ataccaattg tagtgtgttt agcttgttac tcactaataa tgatagaaac
     7381 tcgaaataag atgtttaact tttatttaag taataactgg gatttaacaa aagaaattaa
     7441 agcgaaaatt ttcataatat ctttcgcttt tttaatacac ggccttatga tgtatatagt
     7501 agcgcttgta ggtgattttt tgataagtcc tagtcctata aaaccttctt tattattagc
     7561 aactatatta ataatttttt gggtggctat cccattaatt cctttgaatt ttttgttgct
     7621 taggtaccta gggatttttc ttactatatt tgtaaattta gtattatcta tatttagtat
     7681 tatattttta acactaaaga gtgtattttg gctatctcca tgggctataa tgcaaaaaat
     7741 cccccttatt actctcggta tacttcctaa tggcctaaca gtcgataaat attctaaata
     7801 ttttaacgat ttagagtccc tatatatttc tataattgtt tcaattgtca catatataat
     7861 atttacaatt ttaaacacta aaaaaagttg gagattataa tgattaatga attaagagta
     7921 tgcaacttga aattttctaa tcaattttta acatacttac cgatactaat aagttttata
     7981 tttgtttttt ttataaattt ttatattaat tctaaaactt gggatgacga tcagataagt
     8041 ctttatacat ctagctttaa tgtaatggtc tctatatttc tgtccataaa tgtgtaccaa
     8101 gtaatcaatt ttgaagaaaa tattggtcat tttaatcata ttctaagtaa acctaaaagg
     8161 ataaattggg ctctttcagt attgtttttc tcgtattcaa ttacttcact ttgtataatt
     8221 atagctgtca taaatttatt tatacacaca aataatgtat tcattacatt gtttttcttc
     8281 gtttcttctt ttgtttttaa tataataaca ttaataatat tgtttatatt cggtgttttt
     8341 tttaagagta ttcttagtat cattattgga atcttaaatt ttatatttaa tgtctacttt
     8401 ggattagaag ttttaggaga tcattcttgg ttttatatgc caacaacata ttctacacgt
     8461 tatattcata tgtatataga aaataacata cctatgacat taaccattat aatgtatatt
     8521 gtgtctacaa ttatattatt ttgttttata tatattagat ttaacaagtg gaatggtagg
     8581 gttattaaag actaa
//
