BGC0001630 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001630 (uncultured Candidatus Entotheonella sp)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS,NRPS-like	1	36,804	kasumigamide	NRPS:Type I+PKS	100%

BGC0001630 - Region 1 - NRPS,NRPS-like,T1PKS

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Location: 1 - 36,804 nt. (total: 36,804 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 36,805
Label		BGC0001630

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001630	2.05	NRP, Polyketide	kasumigamide	uncultured Candidatus Entotheonella sp.
BGC0001133	1.01	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002437	0.97	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0000426	0.95	NRP	sevadicin	Paenibacillus larvae
BGC0002071	0.92	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	0.91	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.87	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001833	0.84	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132	0.83	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825	0.82	NRP	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001630	0.94	NRP, Polyketide	kasumigamide	uncultured Candidatus Entotheonella sp.
BGC0001133	0.71	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000426	0.70	NRP	sevadicin	Paenibacillus larvae
BGC0002071	0.68	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002437	0.67	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0002135	0.64	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000351	0.63	NRP	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0002518	0.62	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001023	0.62	NRP, Polyketide	myxochromide S1	Stigmatella aurantiaca
BGC0001833	0.62	NRP	icosalide A, icosalide B	Burkholderia gladioli

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 36804:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (bAla - Trp - ohmal) + (D-Arg - D-X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 27548:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (bAla - Trp - ohmal) + (D-Arg - D-X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

kasA

AMP-binding (178..593): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: --LWVAASGK
Nearest Stachelhaus code(s):: Cin AILWVAASGK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

kasB

Search NORINE for peptide: strict or relaxed

AMP-binding (502..904): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: VDWVVSLADK
Nearest Stachelhaus code(s):: bAla VDWVVSLADK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1568..1959): Trp

nrpys: Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVASVGAVVK
Nearest Stachelhaus code(s):: Trp DVASVGAVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (2688..2983): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 70.8%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 166.8
inactive: 96.3
Methoxymalonyl-CoA: 69.3
Methylmalonyl-CoA: 67.8
Propionyl-CoA: 33.9
2-Methylbutyryl-CoA: 33.1
Ethylmalonyl-CoA: 31.9
CHC-CoA: 27.8
Isobutyryl-CoA: 23.6
fatty_acid: 23.5
Benzoyl-CoA: 18.0
trans-1,2-CPDA: 13.2
Acetyl-CoA: 12.6
3-Methylbutyryl-CoA: 6.4

kasC

Search NORINE for peptide: strict or relaxed

AMP-binding (537..944): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVENVGAITK
Nearest Stachelhaus code(s):: Arg DVENVGAITK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2080..2476): X

nrpys: R-bohPhe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTIAAVCK
Nearest Stachelhaus code(s):: R-bohPhe DAWTIAAVCK (100% 8Å match); Phe DAWTIAAVCK (97% 8Å match)
Stachelhaus code match:: 100% (strong)