BGC0001635 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001635 (Nonomuraea sp. ATCC 55076)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T3PKS	1	70,700	High	kistamicin A	NRPS:Type I+PKS

BGC0001635 - Region 1 - NRPS,T3PKS

Download region GenBank file

Download region SVG

Location: 1 - 70,700 nt. (total: 70,700 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 70,701
Label		BGC0001635

Tailoring functions

Oxidoreductases: oxygenase

BKM31_54160: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

BKM31_54165: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: aminotransferase halogenase

BKM31_54170: aminotransferase

smcogs: SMCOG1019: aminotransferase

BKM31_54195: flavin-dependent tyrosine-like hpg halogenase

halogenases: flavin-dependent tyrosine-like hpg halogenase
MITE: MITE0000028Halogenation (identity: 76.30%)
smcogs: SMCOG1119: halogenase

Lyases: hydratase

BKM31_54180: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

BKM31_54185: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

BKM31_54190: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

BKM31_54135: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BKM31_54140: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BKM31_54145: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BKM31_54175: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001635.4	1.39	NRPS, PKS	kistamicin A	Nonomuraea sp. ATCC 55076
BGC0002341.2	0.94	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.92	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001635.4	0.98	NRPS, PKS	kistamicin A	Nonomuraea sp. ATCC 55076
BGC0002341.2	0.77	other (other)	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002608.2	0.76	NRPS, PKS	ochratoxin A	Aspergillus steynii
BGC0002135.2	0.71	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001050.5	0.70	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0000322.3	0.69	NRPS	chloroeremomycin	Amycolatopsis orientalis
BGC0001825.4	0.69	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 70700:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Trp) + (D-Dhpg) + (Hpg - D-Hpg - Tyr) + (Hpg)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 65291:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Trp) + (D-Dhpg) + (Hpg - D-Hpg - Tyr) + (Hpg)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 29611 - 70700:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Hpg - D-Hpg - Tyr) + (Hpg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BKM31_54135: Trp

Search NORINE for peptide: strict or relaxed

AMP-binding (457..841): Trp

nrpys: Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVGIVGEITK
Nearest Stachelhaus code(s):: Trp DVGIVGEITK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BKM31_54140: Dhpg

Search NORINE for peptide: strict or relaxed

AMP-binding (448..832): Dhpg

nrpys: Dhpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: dhpg (Dhpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DAHQAGTLYK
Nearest Stachelhaus code(s):: Dhpg DAHQAGTLYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BKM31_54145: Hpg - Hpg - Tyr

Search NORINE for peptide: strict or relaxed

AMP-binding (458..842): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DIYHLGLLCK
Nearest Stachelhaus code(s):: Hpg DIYHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1488..1878): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DIYHLGMLCK
Nearest Stachelhaus code(s):: Hpg DIYHLGMLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2992..3376): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTVAAVCK
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BKM31_54150: Hpg

Search NORINE for peptide: strict or relaxed

AMP-binding (451..848): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: Polar, uncharged (hydroxy-phenyl) (Dhpg, Hpg)
Single AA prediction:: hpg (Hpg)

Stachelhaus prediction details:

Stachelhaus sequence:: DAYHLGLLCK
Nearest Stachelhaus code(s):: Hpg DAYHLGLLCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor