BGC0001648 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001648 (Streptomyces sp)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	76,167	High	lasalocid	PKS

BGC0001648 - Region 1 - T1PKS

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Location: 1 - 76,167 nt. (total: 76,167 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 76,168
Label		BGC0001648

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

ANR02547.1: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

ANR02548.1: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

ANR02550.1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

ANR02553.1: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

ANR02555.1: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

ANR02562.1: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

Transferases: acylase

ANR02549.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ANR02551.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

ANR02552.1: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

ANR02545.1: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: synthase

ANR02544.1: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

ANR02554.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

ANR02556.1: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

ANR02557.1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001648.5	1.00	PKS	lasalocid	Streptomyces sp.
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000086.5	0.70	PKS	lasalocid	Streptomyces lasaliensis
BGC0000087.5	0.68	PKS	lasalocid	Streptomyces lasaliensis
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.67	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001648.5	1.00	PKS	lasalocid	Streptomyces sp.
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000086.5	0.70	PKS	lasalocid	Streptomyces lasaliensis
BGC0000087.5	0.68	PKS	lasalocid	Streptomyces lasaliensis
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000041.3	0.67	PKS	oronofacic acid	Pseudomonas syringae
BGC0002154.2	0.67	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0001257.3	0.67	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 76167:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Me-mal) + (Me-ohmal) + (redmal - pk) + (redmal) + (pk - Me-redmal - ohmal) + (Me-ccmal) + (mal)
Non-colinear ordering:: ANR02549.1 + ANR02551.1 + ANR02552.1 + ANR02553.1 + ANR02554.1 + ANR02555.1 + ANR02556.1 + ANR02557.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ANR02549.1: mal

PKS_AT (540..839): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.3
inactive: 110.0
Methoxymalonyl-CoA: 83.0
Methylmalonyl-CoA: 63.9
Propionyl-CoA: 52.8
Isobutyryl-CoA: 43.2
Ethylmalonyl-CoA: 28.2
Acetyl-CoA: 25.8
2-Methylbutyryl-CoA: 24.0
fatty_acid: 21.4
Benzoyl-CoA: 20.4
CHC-CoA: 18.4
3-Methylbutyryl-CoA: 8.7
trans-1,2-CPDA: 5.4

ANR02551.1: mmal

PKS_AT (564..861): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 194.6
Ethylmalonyl-CoA: 179.2
Methoxymalonyl-CoA: 157.0
2-Methylbutyryl-CoA: 145.0
trans-1,2-CPDA: 120.6
Propionyl-CoA: 101.1
CHC-CoA: 94.3
Malonyl-CoA: 74.6
Isobutyryl-CoA: 63.4
Benzoyl-CoA: 53.0
Acetyl-CoA: 32.3
fatty_acid: 28.0
3-Methylbutyryl-CoA: 27.0
inactive: 14.0

ANR02552.1: mmal

PKS_AT (569..866): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 192.8
Ethylmalonyl-CoA: 177.1
Methoxymalonyl-CoA: 157.0
2-Methylbutyryl-CoA: 144.0
trans-1,2-CPDA: 112.5
CHC-CoA: 100.9
Propionyl-CoA: 100.4
Malonyl-CoA: 73.2
Isobutyryl-CoA: 63.9
Benzoyl-CoA: 50.9
Acetyl-CoA: 26.7
3-Methylbutyryl-CoA: 25.3
fatty_acid: 20.3
inactive: 12.8

ANR02553.1: mal - pk

PKS_AT (563..849): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 172.5
inactive: 83.8
Methoxymalonyl-CoA: 41.3
Methylmalonyl-CoA: 27.9
Propionyl-CoA: 26.6
Acetyl-CoA: 16.0
fatty_acid: 15.7
Isobutyryl-CoA: 13.3
Benzoyl-CoA: 11.7
2-Methylbutyryl-CoA: 9.7
trans-1,2-CPDA: 1.6
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2623..2916): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 183.3
Ethylmalonyl-CoA: 173.9
Methoxymalonyl-CoA: 145.9
2-Methylbutyryl-CoA: 118.6
trans-1,2-CPDA: 104.9
CHC-CoA: 94.6
Propionyl-CoA: 91.6
Malonyl-CoA: 77.2
Isobutyryl-CoA: 57.2
Benzoyl-CoA: 37.7
fatty_acid: 33.4
3-Methylbutyryl-CoA: 21.7
inactive: 20.2
Acetyl-CoA: 17.6

ANR02554.1: mal

PKS_AT (1312..1600): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.1
inactive: 94.4
Methoxymalonyl-CoA: 41.9
Methylmalonyl-CoA: 30.3
Propionyl-CoA: 29.6
fatty_acid: 16.1
Isobutyryl-CoA: 15.8
Acetyl-CoA: 15.8
Benzoyl-CoA: 12.0
CHC-CoA: 8.3
Ethylmalonyl-CoA: 7.4
2-Methylbutyryl-CoA: 6.5
trans-1,2-CPDA: 1.1
3-Methylbutyryl-CoA: 0.0

ANR02555.1: pk - mmal - mal

PKS_AT (566..859): pk

ATSignature: Ethylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 87.5%; Methylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 184.8
Ethylmalonyl-CoA: 175.9
Methoxymalonyl-CoA: 147.3
2-Methylbutyryl-CoA: 120.2
trans-1,2-CPDA: 104.7
CHC-CoA: 95.8
Propionyl-CoA: 91.1
Malonyl-CoA: 80.4
Isobutyryl-CoA: 59.5
Benzoyl-CoA: 39.5
fatty_acid: 33.8
3-Methylbutyryl-CoA: 22.7
inactive: 20.0
Acetyl-CoA: 19.6

PKS_AT (2329..2626): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 193.5
Ethylmalonyl-CoA: 177.4
Methoxymalonyl-CoA: 157.8
2-Methylbutyryl-CoA: 144.8
trans-1,2-CPDA: 114.2
CHC-CoA: 101.8
Propionyl-CoA: 101.0
Malonyl-CoA: 73.5
Isobutyryl-CoA: 64.2
Benzoyl-CoA: 51.4
Acetyl-CoA: 26.4
3-Methylbutyryl-CoA: 26.2
fatty_acid: 21.0
inactive: 12.9

PKS_AT (4392..4680): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.6
inactive: 85.8
Methoxymalonyl-CoA: 40.0
Methylmalonyl-CoA: 29.0
Propionyl-CoA: 22.9
fatty_acid: 17.5
Acetyl-CoA: 16.0
Isobutyryl-CoA: 15.4
Benzoyl-CoA: 11.6
2-Methylbutyryl-CoA: 10.0
trans-1,2-CPDA: 1.3
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

ANR02556.1: mmal

PKS_AT (601..898): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 192.8
Ethylmalonyl-CoA: 177.1
Methoxymalonyl-CoA: 157.0
2-Methylbutyryl-CoA: 144.0
trans-1,2-CPDA: 112.5
CHC-CoA: 100.9
Propionyl-CoA: 100.4
Malonyl-CoA: 73.2
Isobutyryl-CoA: 63.9
Benzoyl-CoA: 50.9
Acetyl-CoA: 26.7
3-Methylbutyryl-CoA: 25.3
fatty_acid: 20.3
inactive: 12.8

ANR02557.1: mal

PKS_AT (566..854): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.5
inactive: 94.6
Methoxymalonyl-CoA: 40.1
Methylmalonyl-CoA: 29.3
Propionyl-CoA: 26.4
Isobutyryl-CoA: 16.2
fatty_acid: 16.0
Acetyl-CoA: 15.8
Benzoyl-CoA: 12.0
2-Methylbutyryl-CoA: 8.4
CHC-CoA: 8.3
Ethylmalonyl-CoA: 7.4
trans-1,2-CPDA: 1.1
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor