BGC0001658 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001658 (Amycolatopsis sp. M39)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	53,529	macrotermycins	PKS	100%

BGC0001658 - Region 1 - T1PKS

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Location: 1 - 53,529 nt. (total: 53,529 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 53,530
Label		BGC0001658

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001658	1.00	Polyketide	macrotermycins	Amycolatopsis sp. M39
BGC0000016	0.39	Other	amphotericin B	Streptomyces nodosus
BGC0001163	0.33	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001164	0.33	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000068	0.32	Polyketide	geldanamycin	Streptomyces hygroscopicus
BGC0001273	0.31	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276	0.31	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001068	0.31	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001244	0.31	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0002366	0.30	Polyketide	enterocin, neoenterocin A, neoenterocin B	Streptomyces sp. SCSIO 11863

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001658	1.00	Polyketide	macrotermycins	Amycolatopsis sp. M39
BGC0000016	0.71	Other	amphotericin B	Streptomyces nodosus
BGC0001163	0.67	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001164	0.66	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000068	0.65	Polyketide	geldanamycin	Streptomyces hygroscopicus
BGC0001273	0.65	Polyketide	asperlactone	Aspergillus ochraceus
BGC0001276	0.65	Polyketide	6-methylsalicyclic acid	Aspergillus terreus
BGC0001068	0.64	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.
BGC0001244	0.64	Polyketide	(-)-Mellein	Parastagonospora nodorum
BGC0002366	0.64	Polyketide	enterocin, neoenterocin A, neoenterocin B	Streptomyces sp. SCSIO 11863

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 53529:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccmal) + (Me-ohmal) + (ccmal - ccmal) + (mal)
Non-colinear ordering:: A4R44_03195 + A4R44_03196 + A4R44_03197 + A4R44_03191

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

A4R44_03178

PKS_AT (46..285): pk

ATSignature: (unknown)

No matches above 50%

minowa: trans-1,2-CPDA

Prediction, score:

trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

A4R44_03180

AMP-binding (17..401): X

nrpys: bMe-Asp, Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DCFFFALITK
Nearest Stachelhaus code(s):: bMe-Asp DCFFFALITK (91% 8Å match); Ala DCFFFALITK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

A4R44_03181

AMP-binding (12..394): X

nrpys: 3S-Abu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DYYPTTQRIK
Nearest Stachelhaus code(s):: 3S-Abu DYYPTTQRIK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

A4R44_03191

PKS_AT (1762..2048): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 127.6
Methylmalonyl-CoA: 58.5
Methoxymalonyl-CoA: 57.8
inactive: 50.2
Ethylmalonyl-CoA: 23.5
Isobutyryl-CoA: 19.3
fatty_acid: 13.2
Propionyl-CoA: 11.4
Acetyl-CoA: 9.6
CHC-CoA: 5.5
trans-1,2-CPDA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

A4R44_03195

PKS_AT (550..830): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 197.7
inactive: 101.3
Methoxymalonyl-CoA: 71.4
Methylmalonyl-CoA: 60.0
Propionyl-CoA: 30.7
Isobutyryl-CoA: 27.5
CHC-CoA: 24.6
Acetyl-CoA: 19.2
Benzoyl-CoA: 18.1
2-Methylbutyryl-CoA: 14.8
Ethylmalonyl-CoA: 13.1
trans-1,2-CPDA: 10.2
fatty_acid: 10.1
3-Methylbutyryl-CoA: 0.0

A4R44_03196

PKS_AT (551..850): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 197.5
Ethylmalonyl-CoA: 182.2
Methoxymalonyl-CoA: 156.5
2-Methylbutyryl-CoA: 115.1
trans-1,2-CPDA: 114.9
Propionyl-CoA: 102.5
Malonyl-CoA: 96.2
Isobutyryl-CoA: 92.3
CHC-CoA: 85.1
Benzoyl-CoA: 68.1
3-Methylbutyryl-CoA: 56.5
Acetyl-CoA: 55.9
inactive: 28.2
fatty_acid: 26.7

A4R44_03197

PKS_AT (557..841): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.2
inactive: 118.6
Methoxymalonyl-CoA: 58.2
Methylmalonyl-CoA: 43.3
Propionyl-CoA: 26.0
Benzoyl-CoA: 24.3
CHC-CoA: 20.4
Isobutyryl-CoA: 18.5
Acetyl-CoA: 17.2
2-Methylbutyryl-CoA: 16.9
Ethylmalonyl-CoA: 11.6
fatty_acid: 10.4
trans-1,2-CPDA: 9.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (2225..2503): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 204.3
inactive: 96.6
Methoxymalonyl-CoA: 67.9
Methylmalonyl-CoA: 63.9
Isobutyryl-CoA: 44.1
Ethylmalonyl-CoA: 28.1
Acetyl-CoA: 27.6
Propionyl-CoA: 27.3
CHC-CoA: 18.8
2-Methylbutyryl-CoA: 18.4
Benzoyl-CoA: 17.6
trans-1,2-CPDA: 10.1
fatty_acid: 5.9
3-Methylbutyryl-CoA: 0.0