BGC0001662 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001662 (Kitasatospora mediocidica)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	168,396	High	mediomycin A	PKS

BGC0001662 - Region 1 - T1PKS

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Location: 1 - 168,396 nt. (total: 168,396 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 168,397
Label		BGC0001662

Tailoring functions

Oxidoreductases: dehydrogenase

cle3: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

cle9: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase sulfotransferase

cle7: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

cleB: Sulfotransferase domain

extras: Sulfotransfer_3: Sulfotransferase domain

cleG: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

cleF: thioesterase

smcogs: SMCOG1004: thioesterase

cleN: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: hydratase

cleO: Nitrilase/cyanide hydratase and apolipoprotein

smcogs: SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein

Ligases: ligase synthase

cle1: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

cle2: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

cle4: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

cle5: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

cle6: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

cle8: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

cleE: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001662.4	1.00	PKS	mediomycin A	Kitasatospora mediocidica
BGC0002356.3	0.92	PKS, other (other)	mediomycin A	Streptomyces blastmyceticus
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002357.3	0.76	PKS, other (other)	neomediomycin B	Streptomyces sp. RK95-74
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0002355.2	0.68	PKS, other (other)	tetrafibricin	Streptomyces neyagawaensis
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001662.4	1.00	PKS	mediomycin A	Kitasatospora mediocidica
BGC0002356.3	0.92	PKS, other (other)	mediomycin A	Streptomyces blastmyceticus
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0002357.3	0.76	PKS, other (other)	neomediomycin B	Streptomyces sp. RK95-74
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0002355.2	0.68	PKS, other (other)	tetrafibricin	Streptomyces neyagawaensis
BGC0000107.3	0.66	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 168396:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ohmal - ohmal - ccmal - ohmal - ohmal) + (ccmal - ohmal) + (ccmal - ohmal - ccmal - ohmal - ccmal) + (Me-ohmal) + (ohmal - ccmal - ccmal) + (ccmal - ccmal - ccmal - ccmal) + (Me-ohmal - Me-ohmal) + (ccmal - redmal - ccmal) + (ccmal - Me-ccmal)
Non-colinear ordering:: cle1 + cle2 + cle3 + cle4 + cle5 + cle6 + cle7 + cle8 + cle9

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

cle1: mal - mal - mal - mal - mal

PKS_AT (670..966): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 237.3
inactive: 137.8
Methoxymalonyl-CoA: 80.9
Methylmalonyl-CoA: 67.7
Isobutyryl-CoA: 41.9
Propionyl-CoA: 40.8
Acetyl-CoA: 26.7
Benzoyl-CoA: 23.8
Ethylmalonyl-CoA: 22.9
2-Methylbutyryl-CoA: 19.1
trans-1,2-CPDA: 18.2
fatty_acid: 11.2
CHC-CoA: 11.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (2211..2502): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 235.8
inactive: 119.9
Methoxymalonyl-CoA: 67.8
Methylmalonyl-CoA: 60.3
Propionyl-CoA: 43.1
Benzoyl-CoA: 25.2
Isobutyryl-CoA: 23.9
Acetyl-CoA: 22.2
Ethylmalonyl-CoA: 20.8
2-Methylbutyryl-CoA: 19.5
trans-1,2-CPDA: 12.7
CHC-CoA: 12.1
fatty_acid: 9.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (3739..4030): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 233.6
inactive: 122.6
Methoxymalonyl-CoA: 66.8
Methylmalonyl-CoA: 60.8
Propionyl-CoA: 41.0
Isobutyryl-CoA: 24.5
Acetyl-CoA: 24.0
Benzoyl-CoA: 23.6
2-Methylbutyryl-CoA: 18.6
Ethylmalonyl-CoA: 15.6
trans-1,2-CPDA: 13.2
CHC-CoA: 12.2
fatty_acid: 9.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (5516..5807): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 237.4
inactive: 123.4
Methoxymalonyl-CoA: 69.6
Methylmalonyl-CoA: 68.1
Propionyl-CoA: 44.6
Benzoyl-CoA: 26.7
Acetyl-CoA: 26.4
Ethylmalonyl-CoA: 26.1
Isobutyryl-CoA: 21.8
trans-1,2-CPDA: 20.1
2-Methylbutyryl-CoA: 19.3
CHC-CoA: 16.9
fatty_acid: 11.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (7037..7331): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.1
inactive: 106.5
Methylmalonyl-CoA: 68.7
Methoxymalonyl-CoA: 67.7
Isobutyryl-CoA: 39.2
Propionyl-CoA: 38.7
Acetyl-CoA: 31.8
Ethylmalonyl-CoA: 26.2
2-Methylbutyryl-CoA: 24.0
Benzoyl-CoA: 20.2
CHC-CoA: 12.0
trans-1,2-CPDA: 6.7
fatty_acid: 5.8
3-Methylbutyryl-CoA: 2.6

cle2: mal - mal

PKS_AT (571..854): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 223.3
inactive: 110.5
Methoxymalonyl-CoA: 70.4
Methylmalonyl-CoA: 61.4
Isobutyryl-CoA: 38.4
Propionyl-CoA: 38.3
Ethylmalonyl-CoA: 26.8
2-Methylbutyryl-CoA: 23.3
Acetyl-CoA: 20.0
Benzoyl-CoA: 16.4
CHC-CoA: 15.3
fatty_acid: 14.1
trans-1,2-CPDA: 11.9
3-Methylbutyryl-CoA: 0.0

PKS_AT (2294..2585): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 243.4
inactive: 116.9
Methoxymalonyl-CoA: 68.9
Methylmalonyl-CoA: 62.3
Propionyl-CoA: 41.4
Benzoyl-CoA: 26.2
Acetyl-CoA: 22.1
Isobutyryl-CoA: 22.0
Ethylmalonyl-CoA: 21.5
2-Methylbutyryl-CoA: 15.8
trans-1,2-CPDA: 14.3
CHC-CoA: 12.7
fatty_acid: 9.5
3-Methylbutyryl-CoA: 0.0

cle3: mal - mal - mal - mal - mal

PKS_AT (567..858): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 225.7
inactive: 117.1
Methoxymalonyl-CoA: 63.4
Methylmalonyl-CoA: 62.3
Propionyl-CoA: 46.4
Isobutyryl-CoA: 29.7
2-Methylbutyryl-CoA: 24.8
Acetyl-CoA: 23.0
Ethylmalonyl-CoA: 21.3
Benzoyl-CoA: 19.7
trans-1,2-CPDA: 17.7
fatty_acid: 11.1
CHC-CoA: 10.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (2322..2613): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 233.2
inactive: 123.5
Methoxymalonyl-CoA: 64.4
Methylmalonyl-CoA: 62.6
Propionyl-CoA: 45.2
Isobutyryl-CoA: 31.0
Acetyl-CoA: 28.9
Ethylmalonyl-CoA: 24.9
Benzoyl-CoA: 22.2
trans-1,2-CPDA: 21.5
2-Methylbutyryl-CoA: 15.6
CHC-CoA: 15.2
fatty_acid: 13.8
3-Methylbutyryl-CoA: 0.0

PKS_AT (3798..4089): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 238.9
inactive: 122.5
Methoxymalonyl-CoA: 65.7
Methylmalonyl-CoA: 63.2
Propionyl-CoA: 46.2
Isobutyryl-CoA: 28.5
Ethylmalonyl-CoA: 27.3
Acetyl-CoA: 20.8
2-Methylbutyryl-CoA: 19.1
Benzoyl-CoA: 18.7
CHC-CoA: 15.7
trans-1,2-CPDA: 14.6
fatty_acid: 10.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (5547..5838): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.3
inactive: 123.0
Methoxymalonyl-CoA: 61.3
Methylmalonyl-CoA: 60.4
Propionyl-CoA: 41.6
Isobutyryl-CoA: 30.5
Acetyl-CoA: 28.7
Ethylmalonyl-CoA: 22.8
Benzoyl-CoA: 22.1
trans-1,2-CPDA: 18.9
fatty_acid: 14.0
2-Methylbutyryl-CoA: 13.9
CHC-CoA: 13.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (7083..7375): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 243.5
inactive: 123.5
Methoxymalonyl-CoA: 64.6
Methylmalonyl-CoA: 57.1
Propionyl-CoA: 43.2
Isobutyryl-CoA: 30.0
Benzoyl-CoA: 25.5
Acetyl-CoA: 21.9
Ethylmalonyl-CoA: 17.7
2-Methylbutyryl-CoA: 17.6
trans-1,2-CPDA: 12.6
CHC-CoA: 11.2
fatty_acid: 10.1
3-Methylbutyryl-CoA: 0.0

cle4: mmal

PKS_AT (565..864): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 220.3
Ethylmalonyl-CoA: 204.6
Methoxymalonyl-CoA: 171.9
2-Methylbutyryl-CoA: 141.5
trans-1,2-CPDA: 122.7
Propionyl-CoA: 102.9
Malonyl-CoA: 99.1
CHC-CoA: 90.9
Isobutyryl-CoA: 88.3
Benzoyl-CoA: 64.1
3-Methylbutyryl-CoA: 61.8
Acetyl-CoA: 50.4
fatty_acid: 38.0
inactive: 31.6

cle5: mal - mal - mal

PKS_AT (568..862): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 242.6
inactive: 114.4
Methoxymalonyl-CoA: 72.4
Methylmalonyl-CoA: 65.6
Propionyl-CoA: 44.3
2-Methylbutyryl-CoA: 29.5
Ethylmalonyl-CoA: 26.6
Acetyl-CoA: 26.0
Isobutyryl-CoA: 25.4
Benzoyl-CoA: 18.2
CHC-CoA: 14.4
trans-1,2-CPDA: 9.5
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2111..2402): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 242.2
inactive: 123.3
Methoxymalonyl-CoA: 67.7
Methylmalonyl-CoA: 64.8
Propionyl-CoA: 48.8
Isobutyryl-CoA: 32.8
Ethylmalonyl-CoA: 27.3
2-Methylbutyryl-CoA: 23.8
Benzoyl-CoA: 23.3
Acetyl-CoA: 23.2
trans-1,2-CPDA: 17.5
CHC-CoA: 14.7
fatty_acid: 10.6
3-Methylbutyryl-CoA: 0.0

PKS_AT (3877..4171): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 235.6
inactive: 119.3
Methoxymalonyl-CoA: 70.3
Methylmalonyl-CoA: 65.1
Propionyl-CoA: 46.2
Ethylmalonyl-CoA: 27.2
Acetyl-CoA: 26.9
2-Methylbutyryl-CoA: 26.3
Benzoyl-CoA: 21.9
Isobutyryl-CoA: 21.3
CHC-CoA: 15.7
trans-1,2-CPDA: 14.7
fatty_acid: 8.1
3-Methylbutyryl-CoA: 0.0

cle6: mal - mal - mal - mal

PKS_AT (566..857): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 239.0
inactive: 122.0
Methoxymalonyl-CoA: 70.3
Methylmalonyl-CoA: 68.6
Propionyl-CoA: 47.0
Ethylmalonyl-CoA: 29.9
2-Methylbutyryl-CoA: 28.3
Acetyl-CoA: 26.9
Isobutyryl-CoA: 24.4
Benzoyl-CoA: 22.2
CHC-CoA: 16.3
trans-1,2-CPDA: 14.3
fatty_acid: 9.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (2323..2614): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 239.0
inactive: 124.7
Methoxymalonyl-CoA: 65.8
Methylmalonyl-CoA: 61.5
Propionyl-CoA: 37.4
Isobutyryl-CoA: 22.4
Acetyl-CoA: 21.9
Benzoyl-CoA: 20.9
Ethylmalonyl-CoA: 16.5
CHC-CoA: 12.3
2-Methylbutyryl-CoA: 11.8
trans-1,2-CPDA: 10.7
fatty_acid: 9.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (4076..4366): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 236.0
inactive: 125.3
Methoxymalonyl-CoA: 67.6
Methylmalonyl-CoA: 60.6
Propionyl-CoA: 43.0
Benzoyl-CoA: 27.1
Isobutyryl-CoA: 26.8
Ethylmalonyl-CoA: 26.4
Acetyl-CoA: 26.4
trans-1,2-CPDA: 20.1
CHC-CoA: 16.4
2-Methylbutyryl-CoA: 16.4
fatty_acid: 10.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (5823..6114): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 236.4
inactive: 117.5
Methoxymalonyl-CoA: 69.6
Methylmalonyl-CoA: 61.5
Propionyl-CoA: 43.7
Isobutyryl-CoA: 29.5
Acetyl-CoA: 29.1
Ethylmalonyl-CoA: 28.0
Benzoyl-CoA: 25.1
2-Methylbutyryl-CoA: 18.3
trans-1,2-CPDA: 17.3
CHC-CoA: 15.5
fatty_acid: 10.0
3-Methylbutyryl-CoA: 0.0

cle7: mmal - mmal

PKS_AT (586..885): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.8
Ethylmalonyl-CoA: 202.9
Methoxymalonyl-CoA: 168.8
2-Methylbutyryl-CoA: 139.5
trans-1,2-CPDA: 120.3
Propionyl-CoA: 101.4
Isobutyryl-CoA: 92.9
CHC-CoA: 92.9
Malonyl-CoA: 92.3
Benzoyl-CoA: 66.0
3-Methylbutyryl-CoA: 59.9
Acetyl-CoA: 51.4
fatty_acid: 39.4
inactive: 30.2

PKS_AT (2124..2423): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.0
Ethylmalonyl-CoA: 205.8
Methoxymalonyl-CoA: 168.4
2-Methylbutyryl-CoA: 144.4
trans-1,2-CPDA: 125.9
Propionyl-CoA: 102.9
Malonyl-CoA: 95.4
CHC-CoA: 93.0
Isobutyryl-CoA: 86.7
Benzoyl-CoA: 66.6
3-Methylbutyryl-CoA: 55.1
Acetyl-CoA: 48.5
fatty_acid: 43.8
inactive: 31.2

cle8: mal - mal - mal

PKS_AT (573..864): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.6
inactive: 120.9
Methoxymalonyl-CoA: 68.0
Methylmalonyl-CoA: 61.9
Propionyl-CoA: 40.9
Acetyl-CoA: 31.4
Isobutyryl-CoA: 30.3
Benzoyl-CoA: 28.5
Ethylmalonyl-CoA: 26.4
2-Methylbutyryl-CoA: 23.9
CHC-CoA: 18.6
trans-1,2-CPDA: 17.6
fatty_acid: 16.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (2341..2632): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 237.5
inactive: 118.1
Methoxymalonyl-CoA: 66.3
Methylmalonyl-CoA: 63.7
Propionyl-CoA: 44.6
Benzoyl-CoA: 26.0
Isobutyryl-CoA: 25.3
Ethylmalonyl-CoA: 25.2
Acetyl-CoA: 22.9
2-Methylbutyryl-CoA: 20.7
CHC-CoA: 15.7
trans-1,2-CPDA: 15.1
fatty_acid: 10.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (4420..4710): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 233.3
inactive: 125.4
Methoxymalonyl-CoA: 68.3
Methylmalonyl-CoA: 65.9
Propionyl-CoA: 45.9
Ethylmalonyl-CoA: 32.5
Benzoyl-CoA: 23.4
2-Methylbutyryl-CoA: 22.9
Isobutyryl-CoA: 21.3
Acetyl-CoA: 21.2
CHC-CoA: 16.8
trans-1,2-CPDA: 15.4
fatty_acid: 8.3
3-Methylbutyryl-CoA: 0.0

cle9: mal - mmal

PKS_AT (574..864): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 227.0
inactive: 115.0
Methoxymalonyl-CoA: 63.7
Methylmalonyl-CoA: 63.1
Propionyl-CoA: 47.7
Isobutyryl-CoA: 29.6
Acetyl-CoA: 27.1
2-Methylbutyryl-CoA: 26.8
Benzoyl-CoA: 21.9
trans-1,2-CPDA: 15.1
Ethylmalonyl-CoA: 14.3
CHC-CoA: 11.4
fatty_acid: 8.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (2322..2621): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 220.9
Ethylmalonyl-CoA: 209.6
Methoxymalonyl-CoA: 175.0
2-Methylbutyryl-CoA: 138.9
trans-1,2-CPDA: 126.5
Propionyl-CoA: 102.5
Malonyl-CoA: 93.6
CHC-CoA: 91.2
Isobutyryl-CoA: 86.4
Benzoyl-CoA: 67.4
3-Methylbutyryl-CoA: 64.6
Acetyl-CoA: 49.5
fatty_acid: 41.8
inactive: 32.4

cleG: pk

PKS_AT (11..281): pk

ATSignature: (unknown)

No matches above 50%

minowa: CHC-CoA

Prediction, score:

CHC-CoA: 13.1
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

cleE: X

AMP-binding (9..371): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QYSDCGSAFK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

Link to PARAS substrate predictor