LOCUS       BGC0001668              7544 bp    DNA     linear   PLN 27-FEB-2015
DEFINITION  TPA_asm: Aspergillus nidulans FGSC A4 chromosome VI.
ACCESSION   BGC0001668
VERSION     BGC0001668
KEYWORDS    .
SOURCE      Aspergillus nidulans FGSC A4
  ORGANISM  Aspergillus nidulans FGSC A4
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus; Aspergillus subgen. Nidulantes.
COMMENT     Aspergillus nidulans FGSC A4 CADRE,
            updated by Eurofungbase with contributions from
            CADRE (http://www.cadre-genomes.org.uk),
            AspGD (http://www.aspergillusgenome.org) and
            Ensembl Genomes (http://www.ensemblgenomes.org).
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 03:26:36
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7544
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7544
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[56:3000]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      57..3000
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..7544
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..7544
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001668"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7544
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /subregion_numbers="1"
                     /tool="antismash"
     mRNA            complement(join(1..951,1011..2223,2292..3000))
                     /locus_tag="ANIA_03396"
                     /note="transcript_id=CADANIAT00009643"
                     /old_locus_tag="AN3396.4"
     gene            complement(1..3000)
                     /locus_tag="ANIA_03396"
                     /old_locus_tag="AN3396.4"
     CDS             complement(join(57..951,1011..2223,2292..3000))
                     /NRPS_PKS="Domain: AMP-binding (43-445). E-value: 4.6e-56.
                     Score: 181.9. Matches aSDomain:
                     nrpspksdomains_ANIA_03396_AMP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (583-649). E-value: 1.2e-10.
                     Score: 33.6. Matches aSDomain:
                     nrpspksdomains_ANIA_03396_PP-binding.1"
                     /NRPS_PKS="Domain: Thioesterase (675-922). E-value:
                     4.4e-37. Score: 120.5. Matches aSDomain:
                     nrpspksdomains_ANIA_03396_Thioesterase.1"
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CADANIAG00009643"
                     /db_xref="EnsemblGenomes-Tr:CADANIAT00009643"
                     /db_xref="GOA:Q5B7T4"
                     /db_xref="InterPro:IPR000873"
                     /db_xref="InterPro:IPR001031"
                     /db_xref="InterPro:IPR006162"
                     /db_xref="InterPro:IPR009081"
                     /db_xref="InterPro:IPR020845"
                     /db_xref="InterPro:IPR029058"
                     /db_xref="UniProtKB/TrEMBL:Q5B7T4"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     204.8; E-value: 3e-62)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ANIA_03396"
                     /note="transcript_id=CADANIAT00009643"
                     /old_locus_tag="AN3396.4"
                     /product="NRPS-like enzyme, putative (JCVI)"
                     /protein_id="CBF82791.1"
                     /sec_met_domain="AMP-binding (E-value: 1.1e-60, bitscore:
                     193.0, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 6.7e-12, bitscore:
                     33.6, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MVGSVVEAHRQSVGCLRNLSQLLAWASNTSGGLIFYSREDDVLTS
                     TRISYAELLADAGEKARLIGQITGLSSESIILLHFDTQREVIEWFWAATLAGYLPAIST
                     PFVDDTARRKAHLLHLHAQLNQPVVLTSKRLVPEFLGLEELRLHDVESLLSSAAKDGLI
                     QYLGVQKLAEDVAVLMLTSGSTGSAKAVPLRHGQLLTAIQGKSTHHGTLPGDVFYNWVG
                     LDHVASLTEIHLHALILGSDQVHTAASELLRNSLQFVRLLDTHKVAYTFAPNFFLTKVL
                     DSLRENPTFTADLSSLKALISGGESNVVVTCDKLTRELRRRGVQAEVIRPGFGMTETCA
                     GSIYSRACPSYDIRQSLEFASLGSCIPGMHMRIMSITEPGKLAAPGESGELQVAGPVVF
                     DHYYNDETATRNAFTPDGWFITGDLGWIDDAGNLNLAGRTKDTIIVNGVKWSSTELEAA
                     IEEEAVSGLVRSFTVVVPTRPPGSATEEIAVVYSPAYAPEDYHARYETAQVISKTVSLL
                     TGTKPARLIPLPQSLLEKSSLGKISHSKVRAALESGEYASIERADQLILAQYRQFKWRP
                     AKSDSERAVQKALVEFLQVPAEGINMDDSIYDLGVSSLNLILLRSTLQRMLDPKIDIPL
                     SIILNNPTPGAIARSIDSSRSSLAGYNAIVPLQQHRHGGTPLFCIHPGSGEVLVFVALA
                     AHFPTRPVYALRTRGYGSNEQLFGSIEETVETYATQIRQVQPHGPYAIAGYSLGSTLAF
                     EVAKVLEAQGEEVKFLASIDYPPHIAHYVRDLNWTDVLLHIAFFLELIDEKTMVEVTPY
                     LHTLDRQTALTHILNIGDAERARALAIDTKHLGLISDIAENFRVNVKTYKPQGKVQHLD
                     VFVADPPTYAARDRKDWRENKLGRWVDFCETKVEFHDCPGIHAKMLNREHIAGFAKVFK
                     AAMRRRGV"
     aSDomain        complement(108..848)
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_03396_Thioesterase.1"
                     /evalue="4.40E-37"
                     /label="ANIA_03396_Thioesterase.1"
                     /locus_tag="ANIA_03396"
                     /protein_end="922"
                     /protein_start="675"
                     /score="120.5"
                     /tool="antismash"
                     /translation="PLFCIHPGSGEVLVFVALAAHFPTRPVYALRTRGYGSNEQLFGSI
                     EETVETYATQIRQVQPHGPYAIAGYSLGSTLAFEVAKVLEAQGEEVKFLASIDYPPHIA
                     HYVRDLNWTDVLLHIAFFLELIDEKTMVEVTPYLHTLDRQTALTHILNIGDAERARALA
                     IDTKHLGLISDIAENFRVNVKTYKPQGKVQHLDVFVADPPTYAARDRKDWRENKLGRWV
                     DFCETKVEFHDCPGIHAKMLNREHI"
     aSModule        108..2871
                     /complete
                     /domains="nrpspksdomains_ANIA_03396_AMP-binding.1"
                     /domains="nrpspksdomains_ANIA_03396_PP-binding.1"
                     /domains="nrpspksdomains_ANIA_03396_Thioesterase.1"
                     /final_module
                     /locus_tags="ANIA_03396"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(597..671)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_03396_0004"
                     /evalue="1.60E-11"
                     /label="NRPS-te1"
                     /locus_tag="ANIA_03396"
                     /protein_end="759"
                     /protein_start="734"
                     /score="36.5"
                     /tool="antismash"
                     /translation="QPHGPYAIAGYSLGSTLAFEVAKVL"
     CDS_motif       complement(819..848)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_03396_0003"
                     /evalue="5.90E-04"
                     /label="NRPS-beforete1"
                     /locus_tag="ANIA_03396"
                     /protein_end="685"
                     /protein_start="675"
                     /score="12.7"
                     /tool="antismash"
                     /translation="PLFCIHPGSG"
     aSDomain        complement(join(927..951,1011..1183))
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_03396_PP-binding.1"
                     /evalue="1.20E-10"
                     /label="ANIA_03396_PP-binding.1"
                     /locus_tag="ANIA_03396"
                     /protein_end="649"
                     /protein_start="583"
                     /score="33.6"
                     /tool="antismash"
                     /translation="AVQKALVEFLQVPAEGINMDDSIYDLGVSSLNLILLRSTLQRMLD
                     PKIDIPLSIILNNPTPGAIAR"
     aSDomain        complement(join(1598..2223,2292..2871))
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_03396_AMP-binding.1"
                     /evalue="4.60E-56"
                     /label="ANIA_03396_AMP-binding.1"
                     /locus_tag="ANIA_03396"
                     /protein_end="445"
                     /protein_start="43"
                     /score="181.9"
                     /specificity="consensus: X"
                     /tool="antismash"
                     /translation="TSTRISYAELLADAGEKARLIGQITGLSSESIILLHFDTQREVIE
                     WFWAATLAGYLPAISTPFVDDTARRKAHLLHLHAQLNQPVVLTSKRLVPEFLGLEELRL
                     HDVESLLSSAAKDGLIQYLGVQKLAEDVAVLMLTSGSTGSAKAVPLRHGQLLTAIQGKS
                     THHGTLPGDVFYNWVGLDHVASLTEIHLHALILGSDQVHTAASELLRNSLQFVRLLDTH
                     KVAYTFAPNFFLTKVLDSLRENPTFTADLSSLKALISGGESNVVVTCDKLTRELRRRGV
                     QAEVIRPGFGMTETCAGSIYSRACPSYDIRQSLEFASLGSCIPGMHMRIMSITEPGKLA
                     APGESGELQVAGPVVFDHYYNDETATRNAFTPDGWFITGDLGWIDDAGNLNLAGRTKDT
                     IIV"
     CDS_motif       complement(1694..1780)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_03396_0002"
                     /evalue="2.20E-05"
                     /label="NRPS-A_a6"
                     /locus_tag="ANIA_03396"
                     /protein_end="413"
                     /protein_start="384"
                     /score="16.9"
                     /tool="antismash"
                     /translation="PGESGELQVAGPVVFDHYYNDETATRNAF"
     CDS_motif       complement(2416..2475)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_03396_0001"
                     /evalue="3.80E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="ANIA_03396"
                     /protein_end="195"
                     /protein_start="175"
                     /score="13.5"
                     /tool="antismash"
                     /translation="AVLMLTSGSTGSAKAVPLRH"
     mRNA            complement(join(4001..4380,4456..4523,4605..4978,
                     5219..5413))
                     /locus_tag="ANIA_03395"
                     /note="transcript_id=CADANIAT00009644"
                     /old_locus_tag="AN3395.4"
     gene            complement(4001..5413)
                     /locus_tag="ANIA_03395"
                     /old_locus_tag="AN3395.4"
     CDS             complement(join(4001..4380,4456..4523,4605..4978,
                     5219..5413))
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CADANIAG00009644"
                     /db_xref="EnsemblGenomes-Tr:CADANIAT00009644"
                     /db_xref="GOA:Q5B7T5"
                     /db_xref="UniProtKB/TrEMBL:Q5B7T5"
                     /locus_tag="ANIA_03395"
                     /note="transcript_id=CADANIAT00009644"
                     /old_locus_tag="AN3395.4"
                     /product="conserved hypothetical protein"
                     /protein_id="CBF82793.1"
                     /transl_table=1
                     /translation="MANMPTEDEIAWMQAHINDSTVPDIIACCSICGAASVIILTLRIW
                     SRLQTRRQLVLSDNLVISSVLNILNPILYGVATAFVKWSILALYIAIFPQKNFQYWVYF
                     LCVINCLNAVAIVLVSCLQCRPLEALWNQAVGGTCIDFSIFSLFNTSFNLVLDVAILVS
                     PIKLVMNLNLSQRKKILLALNFGLGGGACVVAAIRLPFARRVGGTSNPSWDMIPGGLCC
                     VVEVAVALLCASLPVYRPLFARMVSSHSTTGNSQQPGRSRDASDYIHSGGQVSTRITAS
                     KRHSSHRGGINITENISMKTHAYVNGRWAPMPDDDDEAYLVPGTKGSRSTTPSNSHV"
     mRNA            join(5847..6412,6479..7544)
                     /locus_tag="ANIA_03394"
                     /note="transcript_id=CADANIAT00009645"
                     /old_locus_tag="AN3394.4"
     gene            5847..7544
                     /locus_tag="ANIA_03394"
                     /old_locus_tag="AN3394.4"
     CDS             join(5847..6412,6479..7544)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CADANIAG00009645"
                     /db_xref="EnsemblGenomes-Tr:CADANIAT00009645"
                     /db_xref="GOA:Q5B7T6"
                     /db_xref="InterPro:IPR001128"
                     /db_xref="InterPro:IPR002401"
                     /db_xref="InterPro:IPR017972"
                     /db_xref="UniProtKB/TrEMBL:Q5B7T6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 152.7; E-value: 2.6e-46)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ANIA_03394"
                     /note="transcript_id=CADANIAT00009645"
                     /old_locus_tag="AN3394.4"
                     /product="cytochrome P450, putative (Eurofung)"
                     /protein_id="CBF82795.1"
                     /sec_met_domain="p450 (E-value: 3.7e-66, bitscore: 211.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MTYFLLILTPDSTKMAIASLILWGIGTLICYLIYQHSTRSAQRLP
                     LPPGPKPLPIVGNLKDFPPDGKPEYQHWIHHKDRYGGISSVTLLGMTLVIIHDKKAAHE
                     LLEQTSGRTSGRPTMVMANKLCGYESIVVCQDYNARFRRCRKFLHQELGTKVSASQFSA
                     AQELEVKRQLVRALNEPEKWLEHFKTTAGATVLKMAYDYAVDYHKPDPLVDLIDRMMTE
                     FSLAAVPMAWAVDIIPALQYLPGAPFKKTARKWRKSIQAAAYIPYRFVQSQMAALTYKP
                     SYVSKLVQLLKGEQSELDHEDEQAIIWSAASLYGAAADTTVITLTTFTLAMILFPDVQR
                     KAQEEIDRVVGNRLPGFKDREKLPYINALVLEALRWWPIAPMGFPHTATEGFEYNGLYI
                     PKGAYLLPAVWWFLHDPEVYDNPEIFDPDRFLEPRNEPTPMTEAFGYGRRICPGRFFAD
                     SSLFLNIAQSLAVFNFKKAVSSDGKEIEIDVKPKPGLLTYPTKFDFRIEPRSERHIQMI
                     RELERQDPLAAGDAEHLESIDNFQPL"
ORIGIN
        1 cgaggtatat agagcgctct taataactgg taaatgatat agaaacggaa gaaaatctaa
       61 acgcccctgc gccgcatcgc tgccttgaag acctttgcaa agccggcaat atgctccctg
      121 ttaagcatct tggcgtgaat accagggcaa tcatggaact cgacttttgt ctcacaaaag
      181 tcgacccagc gcccaagctt gttctctctc caatcctttc gatcgcgggc tgcataggta
      241 ggcgggtctg ccacgaagac gtccagatgc tgcacttttc cttggggctt atacgtcttg
      301 acgttgacgc ggaagttctc ggcgatatcg ctaatgagcc ccagatgctt ggtgtcgatg
      361 gctagggctc tcgcccgctc agcatcgcct atattcagga tgtgtgtcag tgcagtctgt
      421 cggtcgagcg tgtgcaggta aggtgtgact tcgaccatgg tcttctcgtc aataagctca
      481 agaaagaagg caatatgtag cagcacgtcg gtccaattca agtcgcgcac gtagtgggca
      541 atatgcggcg gatagtcaat gctcgccaga aatttgacct cctctccctg cgcttccagc
      601 actttggcta cttcaaaggc cagtgtggat cccaaggagt accctgcgat tgcatacgga
      661 ccatgcggct gaacttggcg aatctgtgtt gcatacgtct ccacagtttc ctcgatggag
      721 ccgaataatt gctcgtttga gccataacct cgagtacgca gcgcgtacac gggccgcgtc
      781 gggaagtgtg cagcaagggc aacgaatacc agaacttcgc cgcttccagg gtggatgcag
      841 aacaacggtg taccaccgtg tctgtgttgc tgcagtggca cgatcgcatt gtatccagct
      901 aaactagagc gggatgagtc aatcgacctt gcgattgctc caggggtcgg actgcggagc
      961 cattaacgaa cagtttgtat ttggtctttg aagggaatgt gggatctcac ttattcaata
     1021 tgatagacaa tgggatatcg atcttggggt ctagcatcct ctgaagcgta gacctcagca
     1081 atatcagatt caacgagctc acacccaagt cgtaaataga atcatccata ttaatcccct
     1141 cagcaggcac ttgcagaaac tcaaccaagg ctttctgcac agctctttca ctgtcagact
     1201 ttgcagggcg ccacttgaac tggcggtatt gcgccagaat caactggtct gcgcgctcaa
     1261 tcgacgcgta ctcgccgctc tcgagtgcag cacgcacctt gctgtgcgat attttaccaa
     1321 gcgacgactt ctccagaagt gactgaggca gggggataag gcgcgcaggc tttgtgcctg
     1381 tcagcagtga gactgtcttg gaaatgacct gcgcggtctc atatctcgcg tgatagtcct
     1441 cgggggcgta cgccggcgag tagacgacag caatttcctc agtggccgag ccaggagggc
     1501 gggtcggcac aactactgtg aacgaacgca ccaggccaga aaccgcttcc tcctcaatag
     1561 ccgcttctag ctcggtcgag ctccatttga caccattgac gatgatggtg tctttggtcc
     1621 gaccagccag gttcaagttg ccggcatcgt cgatccagcc caaatcccca gttatgaacc
     1681 agccatccgg cgtgaaggcg tttctggtcg ccgtctcatc gttgtagtag tggtcaaata
     1741 cgaccggacc tgcgacttgg agctctccag actcgccggg tgcagctagc tttccgggct
     1801 ctgtgatgct cataatacgc atgtgcatgc cggggatgca ggacccaaga ctcgcaaatt
     1861 caagggactg cctgatatca tacgatgggc aagcccgaga gtagatggat cctgcacatg
     1921 tctcggtcat cccgaagccg ggacgaatca cttcggcttg gacacctcga cggcgaagtt
     1981 ccctcgtgag cttgtcgcag gtcacaacca cattagactc cccgccggaa atcagagcct
     2041 taaggctcga caggtctgcc gtgaacgttg ggttttccct caagctgtca agcaccttgg
     2101 ttagaaagaa gttgggcgca aatgtgtagg ccactttgtg agtgtccagc aatcgaacaa
     2161 actgcagtga attgcggagg agctctgatg ccgcagtatg gacttgatcg ctgccaagta
     2221 tcactaagaa tgtgtttatc agatcgcgaa cagtggccgt atcagaggac gatgcgctgg
     2281 gagtgtcata catgcatgta gatgaatctc ggtcaaacta gcaacgtggt ccagaccgac
     2341 ccagttgtaa aagacatcgc ccggtaatgt gccatgatga gtagactttc cctggatagc
     2401 cgtgagaagc tgaccgtgac gcagaggtac tgccttcgca cttcccgtgc tccccgaagt
     2461 caacatgagg actgccacgt cctcagccag tttctggacc ccgagatact ggataaggcc
     2521 gtctttagcg gcagatgaca ggagagactc aacatcatgt agtctgagct cctcgagtcc
     2581 gagaaattca gggactagtc gcttgcttgt gagcacaacc ggctgattaa gctgtgcgtg
     2641 caggtgtagc aagtgggctt tgcgccgtgc tgtatcgtct acaaagggag tagagatggc
     2701 tggcagatag ccagcaagtg tcgcagccca gaaccactcg atgacctctc gctgagtgtc
     2761 aaaatgcaac agaatgatcg attcagacga gaggcctgta atctggccga ttaaacgagc
     2821 cttctcgcct gcatctgcaa gaagctcagc gtacgagata cgagtggacg tgagcacgtc
     2881 atcctctcga gaataaaata tcaggccccc gctggtatta gatgcccagg caagaagctg
     2941 tgacaggttt cggagacacc caaccgattg ccggtgcgcc tcaaccactg agcctaccat
     3001 tgagccgcaa ttcaacgtac gtgaattagt tgaacttgat gtcggattga atctcgcagg
     3061 aaaatgctgg gacgttgact ggggtgcagg ggaaacatct cagcagcagc atcagcaggt
     3121 tcttgtatta ttagatttgg cagtcacctc ttgtcggccg tcctggaagc ctggcctcga
     3181 tcaaggcatt ctgaggtgga gataagccag actctccaaa aacagaacac gttaggccga
     3241 gatcctgcgg accagtcgag tcgtttcctg tcaggtaatc tggccatgtt tagcaaccgc
     3301 tgagtattct ggtgccttgt ggtactggtc tctcctggcc gacgtaaaaa ccgcaatata
     3361 gaaacaccca atccagacct gcaaccatga cactgactaa cttgctccct tcatgtattc
     3421 ccaaggtccc gaacaaaaca aaaagcccac cagagtgaag tacaattgct ggaaatttga
     3481 tagttggact tcctgtgaag aggtcttgtt ggctggaaca gtccaacaac gcaatagtac
     3541 aagctgacgg ccaacatcat cccctgtctg cagatgcaac cctgcagata aaagaaatcc
     3601 tacgtaggtg cgagccaaac ccaattggtt gtttcccaga ccttttagag acacttgcat
     3661 gcaatttggc ccctgggatt catttaaaac actttcaata aaactaccat gatccggatt
     3721 cgcccgatga tcccagcctg aaggagctaa gagctcactt tcttccaact catctcccgc
     3781 tatattagcc ttttgactga aggaggggcg tcccacccgc cgattggcct gtgcttccga
     3841 ggatccgcca gacccacagc gggccgaccc ctcttccaac caatcacctg cggtgatcaa
     3901 tcggggaaat gcccgtctct ggtcgtacga tttcggtacg gggcaggaat gtagagtgga
     3961 ttcagaaaca gtacctttct tttctttctc gcatgatata ctacacatgg ctattgctag
     4021 gtgttgtcga gcgcgatcct tttgtccctg gcaccagata cgcctcatcg tcgtcgtccg
     4081 gcattggtgc ccacctgcca ttcacgtatg catgtgtctt catgctgatg ttctcagtga
     4141 tgttgatacc ccctcgatgg gatgaatgac gcttgctcgc tgtaatccgt gtgctcacct
     4201 gaccaccgct atggatgtag tcgctggcgt cgcgggagcg tcccggctgc tgggagttgc
     4261 ccgtggtcga atgactggac accatgcgcg caaacagggg tcgatagact ggtagcgatg
     4321 cgcaaagaag ggccacggcg acctcgacga cgcagcatag gcctcctgga atcatgtccc
     4381 ctgtcaggag gagtcagtta actgccaaca caatgatcgc taagatcaga tatactcggg
     4441 gccggcataa cctacagctt gggttggatg taccgcccac gcgtctagca aagggaagtc
     4501 ggatggctgc gacgacacag gcgctggagt tgttaggtta gcatcgaggc cgtaatggtg
     4561 tatttacccc aagggattaa tggaacagtg gataggggac tgaccctccg cccaacccga
     4621 agttaagtgc caacaatatt ttctttcgtt gactcaggtt gaggttcatg acaagcttga
     4681 taggcgatac tagtatggcc acatccaaaa ccaggttgaa ggacgtattg aagaggctga
     4741 aaatgctgaa gtcgatgcat gtgcctccca ctgcttggtt ccacagtgct tcgagagggc
     4801 gacattgcag gcaactaaca agaacaattg cgacagcatt gagacagttg atcacgcaga
     4861 ggaaataaac ccagtactgg aagttcttct gcgggaagat ggcgatgtac agggcaagaa
     4921 tactccactt gacgaatgcg gtggccacgc catagaggat aggattcagg atattaagct
     4981 ggtggagccg cgtatgtcag ctctcaaagt aataccataa aaggatttct aatggtttgg
     5041 agccggcgta caattgctag catacgcatg tcagcggggt cagtaattag aattatctgt
     5101 cttccagcgc cgtaacgtgt tgacaaggcg aagacagtac aaaaagctat aaaaaacacc
     5161 tggacggaat agctctcagc gatgaccctt tgttgcttgt caagagcata atactcacca
     5221 ctgagctaat gaccaaattg tcgctgagaa cgagctgacg gcgcgtttgc agccgcgacc
     5281 agatgcgcag cgtcaagatg atgactgagg cagcaccgca gattgagcag caggcgataa
     5341 tgtctggaac cgttgagtcg ttgatgtgcg cctgcatcca cgcgatctca tcctcggttg
     5401 gcatgttggc catggcgggt gattgggatg gtcctctcgg acctgggtgt gtcaaaaaag
     5461 gtcaaattcg aatattcaac aatgtcactg gattcaatcg gttatatctg ttgagatgga
     5521 gaaggtgaag aggggaggag ggggccaaaa gaaggggcta ggaccgcgcc accggtgagg
     5581 gcatgtactc gccgctaatg caacccttgt cctgttcttt gaacgcgatt gcacgcattg
     5641 cggaagctgc ataatgtcaa gacaccagga aggatgttgg cgcccggagt catcggatta
     5701 tctgagaggc ttgccaggat taagtgccag gattaaagct gcggccgaac tctaagatat
     5761 tgcgggtaag accagtgaaa aggggccaca tctgcagggg caatggcctc cgtcctcgac
     5821 ctttctcgag taaacatttg tcctgcatga cttactttct gttgatcttg acacccgatt
     5881 caactaagat ggcgatcgcg tcgttgatct tatgggggat cggcactctc atctgctacc
     5941 tgatatacca gcattcaaca cggagcgcgc agcggctgcc gctccctccc ggccccaagc
     6001 cgctgcctat cgtgggtaac ctgaaagatt ttcctcctga tggcaaacca gagtaccagc
     6061 attggataca ccataaggac cgctatggtg gtatttcttc tgttaccctt ctgggtatga
     6121 cgctcgtcat tattcatgat aagaaagctg cacatgagct cctggagcag acttcaggaa
     6181 ggacctcagg ccggcctaca atggttatgg caaacaagct gtgtggctat gagtctattg
     6241 ttgtttgtca agactataat gcaaggttcc ggcgctgccg caaattccta catcaagaac
     6301 tcggcaccaa agtttctgcg tcacaattca gtgctgccca agagcttgaa gtaaaacgac
     6361 agcttgtacg cgctctgaac gagcctgaaa aatggctgga acatttcaag acgtgagttc
     6421 cgctggatgg ctcttgagtt tggcgattgt cagctaacgg catgcggcac taaattagca
     6481 ctgctggcgc caccgtgttg aaaatggcct atgactatgc cgtcgattac cataagcctg
     6541 atccgttggt cgacttgatc gacagaatga tgaccgagtt ctccctcgcc gcggttccca
     6601 tggcatgggc ggtagatatt attcctgctc tccaatatct tccaggtgca ccattcaaaa
     6661 agacggcgcg gaagtggaga aagtctattc aagcagcagc atatatcccg taccgatttg
     6721 tccagtctca gatggcagcc ttgacttaca agccatcgta cgtctcaaag ctcgtgcagc
     6781 tgctaaaggg agagcagtcc gagttggatc acgaagacga acaggcaatc atttggtcag
     6841 cagccagtct atacggtgct gcggcggata ccacagttat tactcttact acgttcaccc
     6901 tagccatgat cctgtttccc gatgtgcagc gcaaggctca ggaagagatc gaccgcgtag
     6961 tcggaaaccg cctgccaggc tttaaagacc gcgaaaagct gccatatatc aacgcgttag
     7021 tcctagaggc gctgagatgg tggccaattg cacccatggg cttccctcac acagctactg
     7081 agggttttga atacaacggc ctttatatcc ccaagggcgc atacctcctc ccggcagtct
     7141 ggtggttcct acatgatcca gaagtgtatg acaacccgga aatattcgac ccagaccgtt
     7201 tccttgagcc acgaaacgaa cccactccta tgactgaggc ctttggttat ggtcgaagaa
     7261 tctgccctgg ccggttcttc gcggactcaa gcctcttctt gaatattgcg caatcattgg
     7321 cagtctttaa cttcaagaag gcagtgagca gtgacggcaa agagattgag atcgacgtta
     7381 agccaaagcc aggcctcctc acgtatccga ctaagtttga tttccgaatc gagccgagaa
     7441 gcgagaggca catacagatg atcagagagc tggaacgaca agaccctctg gcagcgggcg
     7501 atgctgagca cctggagagt atcgacaatt tccagccctt gtag
//
