BGC0001702 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001702 (Saccharothrix syringae)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	68,204	nocamycin	NRPS:Type I+PKS	100%

BGC0001702 - Region 1 - NRPS,T1PKS

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Location: 1 - 68,204 nt. (total: 68,204 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 68,205
Label		BGC0001702

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001702	1.48	NRP, Polyketide	nocamycin	Saccharothrix syringae
BGC0001164	0.64	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001163	0.61	Polyketide	1-heptadecene	Moorea producens 3L
BGC0002226	0.59	NRP	acu-dioxomorpholine B, acu-dioxomorpholine A	Aspergillus aculeatus ATCC 16872
BGC0002171	0.56	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275	0.56	NRP, Polyketide	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0000844	0.56	Other	clavulanic acid	Streptomyces clavuligerus
BGC0000343	0.53	NRP	enterobactin	Pseudomonas sp. J465
BGC0002157	0.53	NRP, Alkaloid	(-)-ditryptophenaline	Aspergillus flavus
BGC0000217	0.53	Polyketide	daunorubicin	Streptomyces sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001702	1.00	NRP, Polyketide	nocamycin	Saccharothrix syringae
BGC0002631	0.67	RiPP	myxarylin	Pyxidicoccus fallax
BGC0001164	0.66	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000677	0.66	Terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000160	0.65	Polyketide	terreic acid	Aspergillus terreus NIH2624
BGC0001163	0.65	Polyketide	1-heptadecene	Moorea producens 3L
BGC0002229	0.65	Polyketide	6-MSA, aculin A	Aspergillus aculeatus ATCC 16872
BGC0001051	0.65	NRP, Polyketide	thuggacin A	Sorangium cellulosum
BGC0000067	0.64	Polyketide	geldanamycin	Streptomyces hygroscopicus subsp. duamyceticus
BGC0000170	0.64	Polyketide	yanuthone D	Aspergillus niger ATCC 1015

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 68204:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly) + (mal - Me-ohmal - Me-mal - ohmal) + (Me-ccmal) + (Me-ohmal) + (Me-ccmal) + (ccmal - mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 36840:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly) + (mal - Me-ohmal - Me-mal - ohmal) + (Me-ccmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ARS01470.1

Search NORINE for peptide: strict or relaxed

AMP-binding (659..918): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVIWK
Nearest Stachelhaus code(s):: Gly DILQLGVIWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

ARS01473.1

PKS_AT (435..715): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 179.6
inactive: 71.0
Methoxymalonyl-CoA: 59.7
Methylmalonyl-CoA: 58.3
Propionyl-CoA: 34.6
Isobutyryl-CoA: 34.6
Ethylmalonyl-CoA: 29.7
Acetyl-CoA: 20.8
fatty_acid: 20.4
Benzoyl-CoA: 18.9
2-Methylbutyryl-CoA: 18.2
CHC-CoA: 16.1
trans-1,2-CPDA: 14.4
3-Methylbutyryl-CoA: 4.9

PKS_AT (1511..1808): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 226.5
Ethylmalonyl-CoA: 217.3
Methoxymalonyl-CoA: 177.6
2-Methylbutyryl-CoA: 150.7
trans-1,2-CPDA: 134.8
Malonyl-CoA: 126.8
Propionyl-CoA: 120.8
CHC-CoA: 105.8
Benzoyl-CoA: 80.5
Isobutyryl-CoA: 78.1
Acetyl-CoA: 70.9
fatty_acid: 47.9
3-Methylbutyryl-CoA: 45.7
inactive: 39.4

PKS_AT (3008..3298): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 194.8
Ethylmalonyl-CoA: 181.4
Methoxymalonyl-CoA: 156.3
2-Methylbutyryl-CoA: 124.9
Propionyl-CoA: 116.3
Malonyl-CoA: 103.0
trans-1,2-CPDA: 95.0
CHC-CoA: 83.7
Isobutyryl-CoA: 71.8
Acetyl-CoA: 55.8
Benzoyl-CoA: 51.7
inactive: 37.7
3-Methylbutyryl-CoA: 37.3
fatty_acid: 26.6

PKS_AT (3936..4217): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 155.4
Methoxymalonyl-CoA: 73.7
Methylmalonyl-CoA: 72.6
inactive: 54.8
Propionyl-CoA: 50.6
Isobutyryl-CoA: 41.4
Ethylmalonyl-CoA: 35.9
Benzoyl-CoA: 28.8
fatty_acid: 26.4
CHC-CoA: 26.1
2-Methylbutyryl-CoA: 20.1
Acetyl-CoA: 18.4
trans-1,2-CPDA: 14.0
3-Methylbutyryl-CoA: 12.1

ARS01474.1

PKS_AT (550..826): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 202.7
Ethylmalonyl-CoA: 193.9
Methoxymalonyl-CoA: 161.4
2-Methylbutyryl-CoA: 129.3
Propionyl-CoA: 117.6
Malonyl-CoA: 105.5
trans-1,2-CPDA: 101.1
CHC-CoA: 99.7
Isobutyryl-CoA: 79.8
Benzoyl-CoA: 61.7
Acetyl-CoA: 55.9
3-Methylbutyryl-CoA: 43.1
inactive: 33.0
fatty_acid: 27.3

ARS01475.1

PKS_AT (561..851): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 207.9
Ethylmalonyl-CoA: 202.7
Methoxymalonyl-CoA: 169.6
2-Methylbutyryl-CoA: 132.8
Propionyl-CoA: 117.3
trans-1,2-CPDA: 109.3
Malonyl-CoA: 108.5
CHC-CoA: 100.7
Isobutyryl-CoA: 81.8
Benzoyl-CoA: 67.7
Acetyl-CoA: 57.4
3-Methylbutyryl-CoA: 52.0
inactive: 36.2
fatty_acid: 32.5

ARS01476.1

PKS_AT (559..849): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 205.3
Ethylmalonyl-CoA: 200.0
Methoxymalonyl-CoA: 166.0
2-Methylbutyryl-CoA: 128.8
Propionyl-CoA: 110.9
trans-1,2-CPDA: 106.1
Malonyl-CoA: 102.4
CHC-CoA: 100.4
Isobutyryl-CoA: 78.5
Benzoyl-CoA: 69.0
Acetyl-CoA: 56.7
3-Methylbutyryl-CoA: 48.8
inactive: 35.3
fatty_acid: 28.8

ARS01477.1

PKS_AT (557..825): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 177.3
Methoxymalonyl-CoA: 88.0
Methylmalonyl-CoA: 76.0
inactive: 74.3
Propionyl-CoA: 53.1
Isobutyryl-CoA: 48.1
fatty_acid: 46.1
Ethylmalonyl-CoA: 37.1
2-Methylbutyryl-CoA: 31.1
Acetyl-CoA: 28.5
Benzoyl-CoA: 28.0
CHC-CoA: 22.5
trans-1,2-CPDA: 17.5
3-Methylbutyryl-CoA: 9.3

PKS_AT (2180..2464): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 75.0%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 163.8
Methylmalonyl-CoA: 67.2
Methoxymalonyl-CoA: 67.1
inactive: 65.9
Isobutyryl-CoA: 51.6
Propionyl-CoA: 39.7
Ethylmalonyl-CoA: 28.6
2-Methylbutyryl-CoA: 25.7
CHC-CoA: 22.8
Benzoyl-CoA: 18.8
Acetyl-CoA: 18.4
fatty_acid: 16.6
trans-1,2-CPDA: 14.0
3-Methylbutyryl-CoA: 3.2