BGC0001705 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001705 (Nostoc sp. CENA543)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS,NRPS-like	1	48,240	nodularin	NRPS:Type I+PKS	100%

BGC0001705 - Region 1 - NRPS,NRPS-like,T1PKS

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Location: 1 - 48,240 nt. (total: 48,240 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 48,241
Label		BGC0001705

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001705	2.73	NRP, Polyketide	nodularin	Nostoc sp. CENA543
BGC0001016	1.50	NRP, Polyketide	microcystin	Anabaena sp. 90
BGC0000396	1.45	NRP	nodularin	Nostoc sp. 73.1
BGC0001050	1.27	NRP, Polyketide	thalassospiramide A	Tistrella bauzanensis
BGC0002135	1.21	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000465	1.21	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0001133	1.20	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002437	1.15	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001132	1.08	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128	1.08	NRP	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001705	1.00	NRP, Polyketide	nodularin	Nostoc sp. CENA543
BGC0001016	0.80	NRP, Polyketide	microcystin	Anabaena sp. 90
BGC0000465	0.72	NRP	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000396	0.71	NRP	nodularin	Nostoc sp. 73.1
BGC0002135	0.68	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.67	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0000426	0.67	NRP	sevadicin	Paenibacillus larvae
BGC0002437	0.64	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001833	0.64	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128	0.64	NRP	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 48240:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Arg) + (NMe-Thr - X) + (Me-ohmal) + (Me-ccmal - ccmal) + (mal - Glu)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 16498 - 48240:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal - ccmal) + (Me-ohmal) + (NMe-Thr - X) + (Arg)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ndaF

Search NORINE for peptide: strict or relaxed

PKS_AT (561..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 170.3
inactive: 121.5
Methoxymalonyl-CoA: 54.3
Methylmalonyl-CoA: 47.6
Propionyl-CoA: 44.8
Isobutyryl-CoA: 38.8
3-Methylbutyryl-CoA: 21.1
Benzoyl-CoA: 17.7
Acetyl-CoA: 13.0
CHC-CoA: 11.7
fatty_acid: 10.6
2-Methylbutyryl-CoA: 9.7
Ethylmalonyl-CoA: 8.7
trans-1,2-CPDA: 0.0

AMP-binding (2444..2839): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: DPRHSGVVGK
Nearest Stachelhaus code(s):: Glu DPRHSGVVGK (100% 8Å match); Glu or D-Glu DPRHSGVVGK (94% 8Å match); D-Glu DPRHSGVVGK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

ndaD

PKS_AT (574..761): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 42.9
inactive: 34.8
Propionyl-CoA: 16.0
CHC-CoA: 12.3
2-Methylbutyryl-CoA: 11.6
Methylmalonyl-CoA: 10.7
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2632..2916): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 198.5
inactive: 140.3
Methylmalonyl-CoA: 68.9
Methoxymalonyl-CoA: 67.9
Propionyl-CoA: 40.5
Isobutyryl-CoA: 34.8
Ethylmalonyl-CoA: 27.8
Benzoyl-CoA: 25.8
2-Methylbutyryl-CoA: 24.0
Acetyl-CoA: 21.3
trans-1,2-CPDA: 19.9
CHC-CoA: 17.8
fatty_acid: 13.6
3-Methylbutyryl-CoA: 8.3

ndaC

CAL_domain (90..495): pk

minowa: fatty_acid

Prediction, score:

fatty_acid: 20.1
shikimic_acid: 0.0
NH2: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

PKS_AT (1246..1539): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.7
inactive: 131.2
Methylmalonyl-CoA: 77.1
Methoxymalonyl-CoA: 76.5
Propionyl-CoA: 45.0
Isobutyryl-CoA: 39.2
Ethylmalonyl-CoA: 34.7
Acetyl-CoA: 34.5
2-Methylbutyryl-CoA: 31.6
Benzoyl-CoA: 30.9
CHC-CoA: 26.1
trans-1,2-CPDA: 23.0
fatty_acid: 18.9
3-Methylbutyryl-CoA: 14.7

ndaA

Search NORINE for peptide: strict or relaxed

AMP-binding (260..668): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2024..2450): X

nrpys: D-bMe-Asp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DARHVGIFVK
Nearest Stachelhaus code(s):: D-bMe-Asp DARHVGIFVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ndaB

Search NORINE for peptide: strict or relaxed

AMP-binding (477..874): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWSFGLVDK
Nearest Stachelhaus code(s):: Arg DAWSFGLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)