BGC0001709 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001709 (Streptomyces noursei)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS	1	122,844	High	nystatin	PKS

BGC0001709 - Region 1 - T1PKS

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Location: 1 - 122,844 nt. (total: 122,844 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

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TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 122,845
Label		BGC0001709

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

nysK: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

nysL: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

nysN: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acylase aminotransferase glycosyltransferase

nysB: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

nysDI: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

nysDII: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

Hydrolases: esterase

nysE: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

nysDIII: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

nysA: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

nysC: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

nysI: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

nysJ: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001709.5	1.00	PKS	nystatin	Streptomyces albulus
BGC0000115.5	0.83	PKS, saccharide	nystatin A1	Streptomyces noursei ATCC 11455
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000116.5	0.71	PKS	nystatin-like Pseudonocardia polyene A1	Pseudonocardia autotrophica
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001709.5	1.00	PKS	nystatin	Streptomyces albulus
BGC0000115.5	0.83	PKS, saccharide	nystatin A1	Streptomyces noursei ATCC 11455
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000116.5	0.71	PKS	nystatin-like Pseudonocardia polyene A1	Pseudonocardia autotrophica
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001257.3	0.66	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.66	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 122844:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Me-ohmal - Me-ohmal) + (ccmal - ccmal - redmal - ccmal - ccmal - ccmal) + (ccmal - ohmal - Me-ohmal - ohmal - ohmal - ohmal) + (redmal - ohmal - ccmal) + (ccmal)
Non-colinear ordering:: nysA + nysB + nysC + nysI + nysJ + nysK

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

nysI: mal - mal - mmal - mal - mal - mal

PKS_AT (572..867): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.4
inactive: 105.1
Methoxymalonyl-CoA: 57.7
Methylmalonyl-CoA: 45.2
Acetyl-CoA: 17.4
Ethylmalonyl-CoA: 17.3
Benzoyl-CoA: 16.5
CHC-CoA: 13.7
Isobutyryl-CoA: 10.8
trans-1,2-CPDA: 9.9
Propionyl-CoA: 1.3
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: -1.4

PKS_AT (2328..2624): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 224.6
inactive: 94.1
Methoxymalonyl-CoA: 70.2
Methylmalonyl-CoA: 62.9
Isobutyryl-CoA: 41.7
Propionyl-CoA: 34.6
Ethylmalonyl-CoA: 31.7
2-Methylbutyryl-CoA: 23.9
Acetyl-CoA: 22.7
Benzoyl-CoA: 18.0
CHC-CoA: 15.2
fatty_acid: 9.3
trans-1,2-CPDA: 6.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (3879..4177): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 216.6
Ethylmalonyl-CoA: 205.5
Methoxymalonyl-CoA: 176.1
2-Methylbutyryl-CoA: 137.9
trans-1,2-CPDA: 129.8
Propionyl-CoA: 110.1
CHC-CoA: 103.8
Malonyl-CoA: 88.9
Isobutyryl-CoA: 83.5
Benzoyl-CoA: 63.7
fatty_acid: 52.2
3-Methylbutyryl-CoA: 46.6
Acetyl-CoA: 45.0
inactive: 27.1

PKS_AT (5414..5714): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 229.1
inactive: 118.5
Methoxymalonyl-CoA: 66.2
Methylmalonyl-CoA: 52.2
Propionyl-CoA: 30.1
Benzoyl-CoA: 22.5
Isobutyryl-CoA: 19.0
Acetyl-CoA: 16.7
fatty_acid: 15.7
CHC-CoA: 14.8
Ethylmalonyl-CoA: 12.3
2-Methylbutyryl-CoA: 10.1
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (6942..7240): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.1
inactive: 100.1
Methoxymalonyl-CoA: 71.2
Methylmalonyl-CoA: 60.8
Propionyl-CoA: 38.4
Ethylmalonyl-CoA: 34.8
2-Methylbutyryl-CoA: 26.1
Isobutyryl-CoA: 20.2
Acetyl-CoA: 20.1
Benzoyl-CoA: 19.5
fatty_acid: 13.1
trans-1,2-CPDA: 11.9
CHC-CoA: 1.3
3-Methylbutyryl-CoA: 0.0

PKS_AT (8454..8743): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 228.4
inactive: 112.1
Methoxymalonyl-CoA: 61.6
Methylmalonyl-CoA: 53.3
Isobutyryl-CoA: 34.4
Ethylmalonyl-CoA: 26.7
Propionyl-CoA: 26.2
CHC-CoA: 23.0
Benzoyl-CoA: 21.7
Acetyl-CoA: 16.0
2-Methylbutyryl-CoA: 14.6
fatty_acid: 12.2
trans-1,2-CPDA: 9.7
3-Methylbutyryl-CoA: 0.0

nysJ: mal - mal - mal

PKS_AT (570..859): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.2
inactive: 91.5
Methoxymalonyl-CoA: 53.3
Methylmalonyl-CoA: 47.6
Benzoyl-CoA: 20.5
Propionyl-CoA: 19.9
Acetyl-CoA: 19.6
Ethylmalonyl-CoA: 19.0
Isobutyryl-CoA: 13.9
CHC-CoA: 13.3
fatty_acid: 12.9
2-Methylbutyryl-CoA: 9.3
trans-1,2-CPDA: 8.4
3-Methylbutyryl-CoA: 0.0

PKS_AT (2626..2923): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 201.3
inactive: 93.7
Methoxymalonyl-CoA: 64.4
Methylmalonyl-CoA: 61.6
Isobutyryl-CoA: 38.4
Propionyl-CoA: 33.0
Ethylmalonyl-CoA: 30.6
Acetyl-CoA: 25.6
Benzoyl-CoA: 19.6
2-Methylbutyryl-CoA: 19.1
trans-1,2-CPDA: 18.0
CHC-CoA: 11.4
fatty_acid: 11.2
3-Methylbutyryl-CoA: 5.6

PKS_AT (4142..4439): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 216.8
inactive: 103.4
Methylmalonyl-CoA: 53.9
Methoxymalonyl-CoA: 53.6
Propionyl-CoA: 31.3
Isobutyryl-CoA: 30.5
Ethylmalonyl-CoA: 15.2
Benzoyl-CoA: 15.0
2-Methylbutyryl-CoA: 13.7
Acetyl-CoA: 9.8
CHC-CoA: 9.4
fatty_acid: 8.4
trans-1,2-CPDA: 6.4
3-Methylbutyryl-CoA: 0.0

nysK: mal

PKS_AT (565..856): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 207.2
inactive: 82.1
Methoxymalonyl-CoA: 61.3
Methylmalonyl-CoA: 44.7
Propionyl-CoA: 20.9
Ethylmalonyl-CoA: 18.2
CHC-CoA: 16.3
Isobutyryl-CoA: 15.7
Benzoyl-CoA: 12.1
2-Methylbutyryl-CoA: 11.2
Acetyl-CoA: 7.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

nysA: mal

PKS_AT (521..811): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 195.4
inactive: 85.3
Methoxymalonyl-CoA: 59.3
Methylmalonyl-CoA: 52.9
Propionyl-CoA: 31.4
Isobutyryl-CoA: 26.8
Ethylmalonyl-CoA: 25.2
Benzoyl-CoA: 20.0
CHC-CoA: 19.3
2-Methylbutyryl-CoA: 17.9
Acetyl-CoA: 16.7
trans-1,2-CPDA: 5.3
3-Methylbutyryl-CoA: 0.0
fatty_acid: -0.2

nysB: mmal - mmal

PKS_AT (569..867): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 91.7%; Malonyl-CoA: 87.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 213.6
Ethylmalonyl-CoA: 195.4
Methoxymalonyl-CoA: 172.9
2-Methylbutyryl-CoA: 138.7
trans-1,2-CPDA: 123.6
Propionyl-CoA: 104.8
Malonyl-CoA: 96.7
CHC-CoA: 93.2
Isobutyryl-CoA: 81.3
Benzoyl-CoA: 59.7
fatty_acid: 48.4
3-Methylbutyryl-CoA: 44.9
Acetyl-CoA: 43.7
inactive: 27.8

PKS_AT (2117..2418): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 91.7%; Methoxymalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.6
Ethylmalonyl-CoA: 204.2
Methoxymalonyl-CoA: 174.5
2-Methylbutyryl-CoA: 140.2
trans-1,2-CPDA: 134.2
Propionyl-CoA: 113.5
CHC-CoA: 110.3
Malonyl-CoA: 107.5
Isobutyryl-CoA: 79.1
Benzoyl-CoA: 73.0
Acetyl-CoA: 45.2
fatty_acid: 40.5
3-Methylbutyryl-CoA: 39.7
inactive: 25.4

nysC: mal - mal - mal - mal - mal - mal

PKS_AT (544..834): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 75.0%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 222.0
inactive: 98.6
Methoxymalonyl-CoA: 61.3
Methylmalonyl-CoA: 50.3
Propionyl-CoA: 37.0
Ethylmalonyl-CoA: 22.8
Acetyl-CoA: 20.4
Benzoyl-CoA: 19.6
Isobutyryl-CoA: 16.2
2-Methylbutyryl-CoA: 16.0
CHC-CoA: 13.5
trans-1,2-CPDA: 11.2
fatty_acid: 10.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (2290..2580): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 233.4
inactive: 119.6
Methoxymalonyl-CoA: 63.4
Methylmalonyl-CoA: 59.2
Isobutyryl-CoA: 33.1
Propionyl-CoA: 32.2
Ethylmalonyl-CoA: 21.6
Benzoyl-CoA: 20.5
Acetyl-CoA: 20.3
2-Methylbutyryl-CoA: 18.2
CHC-CoA: 14.0
trans-1,2-CPDA: 7.7
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (4031..4335): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 237.1
inactive: 117.2
Methoxymalonyl-CoA: 63.5
Methylmalonyl-CoA: 61.1
Propionyl-CoA: 39.6
Isobutyryl-CoA: 29.9
Ethylmalonyl-CoA: 27.7
2-Methylbutyryl-CoA: 24.8
Benzoyl-CoA: 24.7
Acetyl-CoA: 20.9
fatty_acid: 16.5
CHC-CoA: 15.3
trans-1,2-CPDA: 12.5
3-Methylbutyryl-CoA: 0.0

PKS_AT (6189..6482): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 236.9
inactive: 106.8
Methoxymalonyl-CoA: 59.3
Methylmalonyl-CoA: 52.9
Propionyl-CoA: 28.7
Ethylmalonyl-CoA: 25.0
Benzoyl-CoA: 24.5
Acetyl-CoA: 22.5
Isobutyryl-CoA: 22.1
2-Methylbutyryl-CoA: 19.5
fatty_acid: 18.5
CHC-CoA: 16.6
trans-1,2-CPDA: 15.7
3-Methylbutyryl-CoA: 0.0

PKS_AT (7953..8245): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 237.0
inactive: 104.9
Methoxymalonyl-CoA: 54.6
Methylmalonyl-CoA: 49.5
Propionyl-CoA: 30.3
Ethylmalonyl-CoA: 24.7
Benzoyl-CoA: 24.7
Acetyl-CoA: 23.9
2-Methylbutyryl-CoA: 20.9
Isobutyryl-CoA: 19.9
fatty_acid: 18.3
CHC-CoA: 16.2
trans-1,2-CPDA: 15.1
3-Methylbutyryl-CoA: 0.0

PKS_AT (9738..10031): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 75.0%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 238.0
inactive: 116.1
Methoxymalonyl-CoA: 63.2
Methylmalonyl-CoA: 60.2
Propionyl-CoA: 34.4
Isobutyryl-CoA: 27.4
Ethylmalonyl-CoA: 24.4
Benzoyl-CoA: 24.0
Acetyl-CoA: 23.7
2-Methylbutyryl-CoA: 18.1
fatty_acid: 14.3
CHC-CoA: 14.3
trans-1,2-CPDA: 11.8
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor