BGC0001716 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001716 (Xenorhabdus nematophila AN6/1)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS	1	72,493	High	odilorhabdin NOSO-95A/odilorhabdin NOSO-95B/odilorhabdin NOSO-95C	NRPS:Type I

BGC0001716 - Region 1 - NRPS

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Location: 1 - 72,493 nt. (total: 72,493 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 72,494
Label		BGC0001716

Tailoring functions

Transferases: aminotransferase

XNC2_2376: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Ligases: ligase

XNC2_2370: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

XNC2_2371: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

XNC2_2372: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

XNC2_2373: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001716.4	0.98	NRPS	odilorhabdin NOSO-95A, odilorhabdin NOSO-95B, odilorhabdin NOSO-95C	Xenorhabdus nematophila AN6/1
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.74	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002071.2	0.73	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.71	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.71	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001716.4	0.98	NRPS	odilorhabdin NOSO-95A, odilorhabdin NOSO-95B, odilorhabdin NOSO-95C	Xenorhabdus nematophila AN6/1
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.74	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002071.2	0.73	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.71	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.71	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 72493:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Lys - X) + (Gly - Orn - Pro) + (His) + (Lys - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

XNC2_2370: Lys - X - X

Search NORINE for peptide: strict or relaxed

AMP-binding (530..935): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIESIGTVLK
Nearest Stachelhaus code(s):: Lys DIESIGTVLK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1599..2007): X

nrpys: Arg, ohArg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DIGDLGIIDK
Nearest Stachelhaus code(s):: Arg or ohArg DIGDLGIIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2667..3073): X

nrpys: N6-ohLys, Lys

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIESIGTIVK
Nearest Stachelhaus code(s):: N6-ohLys or Lys DIESIGTIVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

XNC2_2371: His

Search NORINE for peptide: strict or relaxed

AMP-binding (547..943): His

nrpys: His

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSPVIAEVWK
Nearest Stachelhaus code(s):: His DSPVIAEVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

XNC2_2372: Gly - Orn - Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (542..938): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQIGMVWK
Nearest Stachelhaus code(s):: Gly DILQIGMVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1599..2001): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMENSGAVNK
Nearest Stachelhaus code(s):: Orn DMENSGAVNK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2674..3080): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMENVSHVVK
Nearest Stachelhaus code(s):: Pro DMENVSHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

XNC2_2373: Lys - X

Search NORINE for peptide: strict or relaxed

AMP-binding (488..891): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIESIGTVTK
Nearest Stachelhaus code(s):: Lys DIESIGTVTK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1573..1986): X

nrpys: ohDab

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVWQMIGDDK
Nearest Stachelhaus code(s):: ohDab DVWQMIGDDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor