LOCUS       BGC0001727             13202 bp    DNA     linear   BCT 30-JAN-2018
DEFINITION  Paenibacillus polymyxa E681, complete genome.
ACCESSION   BGC0001727
VERSION     BGC0001727
KEYWORDS    .
SOURCE      Paenibacillus polymyxa E681
  ORGANISM  Paenibacillus polymyxa E681
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
COMMENT     On Mar 18, 2015 this sequence version replaced CP000154.1.
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from Dr. Seung-Hwan Park (shpark@kribb.re.kr).
            Annotation modified by submitter.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 16:09:35
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 1552553
            Orig. end    :: 1565755
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..720
                     /codon_start=1
                     /gene="pnlR2"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 187.2; E-value: 5.2e-57)"
                     /gene_kind="regulatory"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:YP_003944370.1"
                     /locus_tag="PPE_01446"
                     /product="transcriptional response regulator"
                     /protein_id="ADM69285.1"
                     /transl_table=11
                     /translation="MEEIAVLIVDDEQGLRDMLTTVLKKEGFLQVKHAATGQEALQVVM
                     ESHMDVIVLDVMLPDSDGFEVCRQMRLYTDAPILFLTARDTDLDKLMGFGIGGDDYITK
                     PFNPLEVVARMKARMKYRKVPTVAMKPALRTLDFGYFRLELESGVLYVEDKQVECPARE
                     WLLLVFLCKHPNRIFSVRQLYEAIWSEAFLGDEKTVVIHISRLRKKIEPDPKHPQFLIN
                     VRGLGYKMMSVKMEERG"
     subregion       1..13202
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..13202
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..720
                     /gene="pnlR2"
                     /locus_tag="PPE_01446"
     gene            717..1787
                     /gene="pnlK"
                     /locus_tag="PPE_01448"
     CDS             717..1787
                     /codon_start=1
                     /gene="pnlK"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 118.2; E-value: 8.9e-36)"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /locus_tag="PPE_01448"
                     /product="sensor histidine kinase"
                     /protein_id="ADM69286.1"
                     /transl_table=11
                     /translation="MNSLVKISFRFMKVTLCFVLAYVLCFVICTILYIFIKSTFGASQN
                     INIDLFWTGLGIVQSVVFIAAYGWYIGKPLVYVIKWIGNIATGEFQAPLSELELPEGKK
                     NQKNSYKPPYQLYKEVFEQMNILSAQLRSNEEERVEIEKRKQQWVAGVSHDLKTPLSYI
                     EGYAAMLTAQEYDWSDEERRSFSMSISEKVTEMKQLIQDLNASMQLKESPLPIQLEKED
                     IVEFLRSAVIDIANHPSAEQYEFSFTSMETTCIMSFDSKLLGRALQNFLINAIIHNPPG
                     THVSMKVNQANDKLHISIEDDGIGMNSKEFMQVSHSQGHGIPIAKSFIEAHNGTLHILS
                     GSKAGTRMEISLPLNI"
     gene            1932..2855
                     /gene="pnlF"
                     /locus_tag="PPE_01449"
     CDS             1932..2855
                     /codon_start=1
                     /gene="pnlF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 179.1; E-value:
                     1.8e-54)"
                     /gene_kind="transport"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:YP_003944707.1"
                     /locus_tag="PPE_01449"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="ADM69287.1"
                     /transl_table=11
                     /translation="MSEYVIHTQQLTRRFGKREFVKRINLQVPGQQIYGFLGPNGAGKT
                     TTIRMLLGLIKSTSGEIEIFGKKLKDQRMEILKDIGSLVESPAYYAHLSAYRNLEIVTT
                     MRGLPTRRIHEVLDLVRLTKDAYRPVKGYSLGMRQRLGIAMALIANPKLLILDEPTNGL
                     DPSGIQEIRELIMSLPGAYGMTVLVSSHLLSEIEQIATYVGIINQGEMIFQGTMKELTA
                     KSKPQLFIETPDPVLAAGALIQHGWTAGTESIEQNEVVTPIVDRQQSSEMIKVLVEHNH
                     PIYRVTEKKKTLEEIFLELTGKERSL"
     gene            2852..3559
                     /gene="pnlG"
                     /locus_tag="PPE_01450"
     CDS             2852..3559
                     /codon_start=1
                     /gene="pnlG"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /locus_tag="PPE_01450"
                     /product="ABC transporter permease"
                     /protein_id="ADM69288.1"
                     /transl_table=11
                     /translation="MILRVIKSEGLKLKKICWWIPLIQGCVLTGMTAVEWYLYFRQGPG
                     GVYAGFAVMFMFLSFVMLLGGTLLASIMAGTEHDTQTWKQLMAMPVPRSYIYLSKIVWI
                     VILQLGTALITVAGMSLIWVLYTNDPIPWRIMLLQPINASLATLPVLAIQLWLSTLFTN
                     QAFPLAFGIFGSIASLFLARTSILWIKILPWSYPALSSPLMKEHMIWVIVALSAGVIFT
                     GLGTLQFTKHEFK"
     gene            3574..4308
                     /gene="pnlE"
                     /locus_tag="PPE_01451"
     CDS             3574..4308
                     /codon_start=1
                     /gene="pnlE"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /locus_tag="PPE_01451"
                     /product="ABC transporter permease"
                     /protein_id="ADM69289.1"
                     /transl_table=11
                     /translation="MGSILRVETIKLQWIMVWILIILDAIINSLMGVLELNDLKQFFSP
                     SWLTLYTYSAQFHSMFFYPLYCGIIASLLCAYEHRDGGWKILLSSPYPRQQIYYAKYIL
                     LILILGLDQLIFIVGYFVSGNIAGAPGEIPWSVVLSTGLGGWLGIFPLAALQLMLSIKI
                     RNFGASLGMSICCVVPNIVITGFHSSIGAWFPFTIPYYIMMPQTATYAPRVEPYSLGLI
                     VIFTFLAYLYGGRKMFVNRDWL"
     gene            complement(4309..5670)
                     /gene="pnlC2"
                     /locus_tag="PPE_01452"
     CDS             complement(4309..5670)
                     /codon_start=1
                     /gene="pnlC2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     362.8; E-value: 3.6e-110)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /locus_tag="PPE_01452"
                     /product="cyclase"
                     /protein_id="ADM69290.1"
                     /sec_met_domain="LANC_like (E-value: 9.3e-60, bitscore:
                     191.7, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIDSFVIQQSDQVRARDISKKIALRLCEPESVYAAVHAEHNVSP
                     ETKYHPWGETSLSHGYPGNIHLLSQWRATDPSYDWNRAIHSNMAQIQSSLAKNGLHDLS
                     LYSGWTGIASSVRVASNHGKYYSNFLQQIHQWMMPLTEQWLQSNTEQLSLHEGVPMQAF
                     DTISGASGIGRYILMNSTDPICTPLVEPILQFLTDLTHPIHMHNRYVPGWYSPQKFQFL
                     DRDKRNYPLGNFNCGMAHGIPGPLALLSVAYSAGIEVKGQYQAIETIAEWLLKYKYEDE
                     YGSYWPHIVPFEAETGSLRFDLERAREAWCYGTPGIACSLFLAGKALHNENLQKLAVKA
                     CVGAISYEIAKREMDSSALCHGLTGLLTISWRMWRHSKEPAFLPLIQQLTNCLLDRFQM
                     DSPLGYLDPEPRSDGSLTWLTKAGMLEGVAGIAAALLSVSENKEIGWDYILMLS"
     gene            5867..6019
                     /gene="pnlA"
                     /locus_tag="PPE_04953"
     CDS             5867..6019
                     /codon_start=1
                     /gene="pnlA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /locus_tag="PPE_04953"
                     /product="lantibiotic paenilan precursor peptide"
                     /protein_id="ADM69291.1"
                     /sec_met_domain="TIGR03731 (E-value: 6.5e-09, bitscore:
                     25.4, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNQFDLDLQVAKNEVASKGVQPASGLICTPSCATGTLNCQVSLS
                     FCKTC"
     gene            6096..7358
                     /gene="pnlC1"
                     /locus_tag="PPE_01453"
     CDS             6096..7358
                     /codon_start=1
                     /gene="pnlC1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     265.6; E-value: 1.2e-80)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /locus_tag="PPE_01453"
                     /product="cyclase"
                     /protein_id="ADM69292.1"
                     /sec_met_domain="LANC_like (E-value: 3.9e-61, bitscore:
                     196.2, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEDLKGRSVEISKYMAQKLIDLSYTEKIVNHTNNLSPSRGNPPW
                     NPGSLADGIPGVFALLSEWGYLEPDQNWAEHVHSQLRYLDANSLLLLPRISLFYGTTGV
                     AYGILLASNNRANYNKLISQLNKYIEVKWGDLPVIGTITEFSRQMDIINGLLGVGRYTL
                     EVAEDKTMCDLSRQLLDTITHFIPEYIHLSSREDSASIHLGLAHGITGILALLVTALQK
                     KIEVEGLHEAIKHIADFLLIQIQEDQYGGYWPVNSQASSMSKREKKVGMREIEGWCHGT
                     FGIAWTLFRAGIECSNEEWKKIAEKAMQSIFCLDDELLNSLSPTFCHGLAGLLHMAHRF
                     YSLTDNIAALRFADQIALKLVNLYDEELPFGYQDLEGTTHVHNVGILQGATGIALSLLD
                     YGIGKEHLKYSGRWSELFLIS"
     gene            7408..8085
                     /gene="pnlR1"
                     /locus_tag="PPE_01454"
     CDS             7408..8085
                     /codon_start=1
                     /gene="pnlR1"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /locus_tag="PPE_01454"
                     /product="transcriptional response regulator"
                     /protein_id="ADM69293.1"
                     /transl_table=11
                     /translation="MKQKFMVISSFRKIKHFFETLSTPENIRIMFYDSLYQAIPEIDEQ
                     CIGVALNQDHITLEDLSYWHSIKEKFTVPFIFLSYHSDDQRQLDLLKQLFSMASSEYEM
                     EREQQAISLSPNVIFDPAGKVIWKQGHEHMLTMMEFRLLMYLLTAKECASTIDHIIDRV
                     WGVDAFTTSNTVYVHIRKLREKIEDDPSHPKILVTCHGIGYKLNIFPKNLEVVSCEETT
                     ALH"
     gene            8060..11314
                     /gene="pnlB"
                     /locus_tag="PPE_01455"
     CDS             8060..11314
                     /codon_start=1
                     /gene="pnlB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     891.8; E-value: 8.7e-270)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /locus_tag="PPE_01455"
                     /product="dehydratase"
                     /protein_id="ADM69294.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 5.5e-194,
                     bitscore: 635.3, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 7.3e-18,
                     bitscore: 54.8, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.2e-56, bitscore:
                     182.0, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRQLHYTDAHKLHNKVRDRDERWFSACDFFLIRAPLLSLDRYFQ
                     VFSDQAVDSQLTLEEITGRLKLMSQEAVVQEAISLASPSLMQFLHYLNDGHALDDKGEL
                     QKYEKTLNSFYRYFIRMTTRTTPFGLFSGVGQGICGKTTDLDIGSYPKHLKRARPDMEW
                     IYKLIQKIEADPAILNQLKVFLNKTVMIKGNRAKLPFRPKHGAAEADDSAEIFNSIRLT
                     DVVRMTFEAAEHPIRVMELKEILLKAFPDATLEIIADYLQQLVEQEFILSELRPPLMDH
                     SPFHYIFEKIKKLDISDPWIHILHEIDALLLQYNQCTIGEGEKVYRKLCDKMLDIISVK
                     TPVQVDVKLDKEVTLPKHVLDEVSLGAEVMCKLYASPKTDELAEYLNRFLEKYGVYQEV
                     PIHELFDEDWGLGIPEMYRSNEKKSMGMEAFKRNAVLFQWVSNAISNHEIELALTDEQL
                     EQLMISDNYDTSPESMEIYYSIFADSPEKVDSGDYSLLLGASAGSNRAGKTFGRFLDMF
                     SEEFVQKFSEIYDHHRCFHPDALLSEMVFMPEQGKISNISLSKNLMAYEIVSGTTSSKD
                     EDHTLCIDDLVVGATHDRFYVKSRRLGKEIIPLQTHMLNYKLSPHIFRFLTDITNKEIG
                     TWSYFDWGPLEQSPFLPRLVYKKIIISAAQWKLSKASLNISESCESKEGEELFQVWRQH
                     WNVPRYVYLTMGDNRLLLDLCNLFHIKELLHEVSKTREGQELLLTECLGNPFHTPVQGT
                     AGRYMGEFVFPLKKKKIKVKPSIPSSQSHIVSQNFIAHTPSSVHRGIFPGGEWLYLKLY
                     GVKERETDLVGLSLGHLLKQPGFKDWSRKFYFLRYVDPEHHIRLRFQGEPDKLWTAGLA
                     QLNQWAAELREEGLITNMVIDTYHPELERYGGQTLMELAEQVFDVDSQWVIAYMKGIRS
                     NAFNLDIEIAAVMNIIHYLSGFGLTIQEQVKWLEERIDCKPHMKAFRDVKKTLITAVES
                     NYEWIRQADTTTQVAFLALVEKRQAMIQQYAQAIILAKNNHELTNHPDDILGSIIHMHL
                     NRLLGVNREREIKCMALAKHTVANLLHRKGVSYVTV"
     gene            11301..13202
                     /gene="pnlT"
                     /locus_tag="PPE_01456"
     CDS             11301..13202
                     /codon_start=1
                     /gene="pnlT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 193; E-value:
                     1e-58)"
                     /gene_kind="transport"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /locus_tag="PPE_01456"
                     /product="paenilan transporter"
                     /protein_id="ADM69295.1"
                     /transl_table=11
                     /translation="MSQSNGQTNDLISLSYKNIFRTFLFWPRIFRLLWDTHPSFLIAIL
                     ITNLLKGAIPAVILLTTRELINSVVAGASGGSFQPVFIAFGVLIGVNLLYDLINIGESF
                     FRKMFQSRLSNQVNIRIIEKAQKMSLQSFEDADVQNMLQRAQGEADFRPFEIFTQILAI
                     ISSVVTLFSTGAILIAWRWWAFLFIFLIPCLSFYSFLRIGQREFNVHFQRAGRQRESWY
                     LSFLVTRDNSFKEVKIFQLGDYLRKRYKTILEGFYREDKVLAVRRSYTSLVFQIVNQGA
                     CAFIIFMAIQATYVGQILVGNLVSLIQGITLTQSTSQSIVQGILSLCQNNLYIKQLFDF
                     LDMPEEAKIQKSVQQLPLSAIHSIEFRNVSFKYPNTEENAISNVSFKLHQGEKLAIVGK
                     NGSGKSTIVKLMTHLYSDFEGEILINGHSIHELDKDSFREKIGVVFQDFVHYEMAVRDN
                     IGFGNVASRENDEAIWTAIDKAGITDLIVGLPDKINSLLGKWFEDGQQLSGGQWQRIAI
                     ARAFMRNADIYVLDEPSSFLDPHAEKEVFQKFDQLIHEKIGVFISHRLSSARMADTIIL
                     MEEGRIVEAGSHERLMDIDGVYAEMFRLQASSYLSQRERGFEEENLSPSLVRTTGGS"
ORIGIN
        1 atggaagaaa ttgcagtact tatagtagac gatgagcagg ggttgcggga tatgctgaca
       61 acggtgttaa aaaaagaagg gttccttcaa gtcaagcatg ctgcaacggg tcaggaagcg
      121 ctgcaagtcg tgatggaatc tcatatggat gtgattgtac ttgatgtcat gctgcctgat
      181 agcgatgggt ttgaggtgtg tcgtcaaatg cgtctatata cggatgcccc tattcttttc
      241 ttgacggcaa gggatacaga cctggacaaa ttaatggggt ttggtatcgg aggggacgat
      301 tatattacca aaccgtttaa cccgctggaa gtcgttgccc gtatgaaggc tcgaatgaaa
      361 tatcggaaag ttccgacagt ggccatgaaa ccggccttac gcactttaga ttttggatat
      421 tttcgtctgg aactagaatc cggtgtgttg tatgtggagg acaagcaggt cgagtgtccg
      481 gcacgtgaat ggctactgct ggtatttctg tgcaagcatc ctaaccgtat tttcagtgta
      541 cgacagcttt acgaggcgat atggagcgag gccttcttgg gtgatgagaa gaccgtagtg
      601 attcatatct cccgcttgcg gaaaaaaata gaacctgatc ccaagcaccc gcaattttta
      661 atcaatgtaa gaggacttgg ttataaaatg atgtcagtca agatggagga acgaggatga
      721 actctctcgt aaagatatcc tttcgtttta tgaaagtaac gttatgcttt gtactggcct
      781 atgtcctatg ttttgttatt tgcacgatct tatatatttt tataaaaagt acatttggag
      841 catctcaaaa tataaatatt gatctattct ggacaggact tggtatcgtg cagagtgttg
      901 tgttcatagc tgcctacgga tggtatatag gcaaaccttt ggtgtatgta attaaatgga
      961 ttggaaatat tgcgacaggg gaattccaag cgccgctaag tgaactggaa ctgccagagg
     1021 gcaaaaaaaa tcagaaaaat agttataaac ctccgtatca attgtataaa gaagtttttg
     1081 aacaaatgaa catcttatcg gctcaattgc ggagcaatga ggaagagaga gtggaaatag
     1141 agaaacgaaa acagcagtgg gtagctggtg taagccatga tctcaaaaca ccattatctt
     1201 atattgaagg atatgcggcc atgctaactg ctcaagagta cgactggtct gatgaagaaa
     1261 gaagaagctt tagtatgagt atcagtgaga aagtgactga aatgaagcag ttgattcagg
     1321 atttgaatgc ttctatgcag cttaaggaaa gtccattgcc aatccaattg gagaaggagg
     1381 acattgtgga gtttctccgt agtgcagtca tagatatcgc taaccatcct tcggccgagc
     1441 aatacgaatt ttcttttacg tcaatggaga cgacatgtat tatgtccttc gatagcaagt
     1501 tattaggcag agcgctgcag aattttttga ttaacgcaat cattcataat cctccaggta
     1561 cacacgtttc catgaaagta aaccaagcga acgataagct tcatatttct atcgaagatg
     1621 acggaatagg aatgaattct aaagagttca tgcaggtatc tcactcccaa gggcatggaa
     1681 tacctattgc caaaagcttt attgaggcgc ataacggaac gcttcatatc ctttcaggta
     1741 gtaaagcggg tactaggatg gagatttcac tgccgctaaa catctaactt tttaacatct
     1801 aacctcacat gttgcagttt gctcgttttt tttgcctgtt gtttagggac tgtttacttg
     1861 atgtttactt tacttcagta aactgtatgt aacttaagct ccagcattag aagtcgagag
     1921 gagatcagga aatgtctgaa tatgtaatac atacacagca gctaactagg cgttttggaa
     1981 aacgggaatt tgtaaaacga ataaatctcc aggtacccgg acagcagatt tatggatttt
     2041 taggacctaa tggggcgggt aaaacaacca cgattcgtat gctgcttggg ctaattaaat
     2101 ccacctccgg ggaaattgag atttttggga aaaagctgaa ggatcagcgt atggaaatat
     2161 tgaaggatat tggttccttg gttgaatcac ctgcctatta tgcccattta tccgcatatc
     2221 gaaatttaga aatcgttaca accatgcgtg gactgccgac ccgaagaatt catgaagtgc
     2281 tggatttagt gagattaacc aaggatgcgt atcgtccggt aaagggatat tccttgggga
     2341 tgcgacaaag actggggatt gctatggcgc ttattgccaa ccccaaatta ttgatacttg
     2401 atgaaccgac gaatggtttg gacccgtctg gtattcagga aattcgggag ttaattatga
     2461 gcctccctgg cgcttacgga atgacggtac tggtttcgag ccatttatta agcgaaattg
     2521 agcagattgc aacctatgta ggaattatta atcagggtga aatgatcttc cagggaacga
     2581 tgaaggaact caccgccaaa agcaagcctc aattattcat tgaaacccca gatcctgttc
     2641 tggctgccgg tgcactgatt cagcatgggt ggacggccgg aactgaatcg attgaacaaa
     2701 atgaggtcgt gactcctatt gtagatcggc aacaatcttc ggagatgatc aaggtgcttg
     2761 tggaacacaa tcatcccatt tatcgggtca cagagaagaa aaaaacattg gaggagatat
     2821 tcctggaact gacaggtaag gagcgaagct tatgatactc cgggttataa agtcagaggg
     2881 gcttaagctg aagaaaatat gctggtggat tcctcttata caggggtgtg tacttacagg
     2941 catgactgct gtggaatggt atctttactt tcggcaagga ccgggaggtg tctacgctgg
     3001 ttttgccgtt atgtttatgt tcctttcgtt tgtgatgctg cttggcggta cacttttggc
     3061 gagtattatg gccggtacag agcatgatac tcaaacatgg aagcagctta tggccatgcc
     3121 tgttcccaga agttatatct atttatccaa aatcgtatgg atcgtgattc tgcagttggg
     3181 taccgccttg atcactgtag caggtatgag cctcatctgg gtattatata caaacgaccc
     3241 cattccttgg cggatcatgc tgttacagcc gattaatgca agcttggcta ccctaccagt
     3301 acttgcaatt caactttggc tgtctacgct tttcactaat caggcattcc cattagcatt
     3361 tggtatattc ggttcaattg caagcttgtt tttggcacgc acaagtattc tgtggattaa
     3421 aatattgcct tggtcgtacc ctgcactatc aagtcctctg atgaaggaac acatgatttg
     3481 ggtgatcgtt gctttaagtg caggagttat ctttacggga cttggtacac tacagtttac
     3541 caagcatgaa ttcaaataga gaggaaaaga tacatgggct ctattttacg cgtggagaca
     3601 ataaagcttc aatggatcat ggtatggatt ctcattatat tggatgcgat cataaattct
     3661 ttaatgggag tcctggagct gaatgatttg aagcagtttt tttcgcccag ttggttaact
     3721 ttatatactt attcagcgca atttcactct atgttttttt atccgctcta ttgtggaatt
     3781 atcgcttctc ttttgtgtgc gtacgagcac cgggatgggg gatggaagat tttattaagc
     3841 agcccgtatc ctcgtcagca aatttattat gcaaaatata tattgcttat tctcatattg
     3901 ggtctggatc agcttatatt tatagtaggt tattttgtaa gtggaaatat agcaggtgct
     3961 ccaggagaaa tcccttggtc tgtcgttctt tctaccggtt tgggagggtg gcttgggatt
     4021 tttcccctgg cagcactaca gcttatgctc tcgataaaaa ttcggaattt tggagcttct
     4081 ctgggaatgt ctatttgttg tgtggttcct aatattgtca taacaggctt tcactcttcc
     4141 ataggtgcgt ggtttccttt tacgattcct tattatatta tgatgccgca aacagcgact
     4201 tatgcaccca gagttgagcc ttacagtctc ggactgattg tcatttttac gtttttagcc
     4261 tatctgtatg gtggaagaaa gatgtttgtg aatagggact ggttgtagtt aggatagcat
     4321 gaggatgtaa tcccaaccga tctctttgtt ctcagaaaca cttaataaag cagctgcaat
     4381 cccagcaacg ccttcaagca tgcccgcttt tgtcagccaa gtcaaagagc catctgacct
     4441 tggctcgggg tccagatatc caagtgggct atccatttga aagcgatcaa gcagacaatt
     4501 cgttaactgt tgaataagcg ggagaaatgc aggctcttta ctgtgacgcc acattcgcca
     4561 agagatggta agtaatccgg taagaccatg acagagtgca gaggagtcca tttcccgctt
     4621 agctatctca taggagattg caccgacaca agctttcaca gccaattttt gcagattctc
     4681 attatggaga gcctttccgg caagaaatag ggaacaggcg atgccgggtg taccgtaaca
     4741 ccatgcttct cttgctcgtt ccaaatcaaa ccgtagagag ccagtttcag cttcgaatgg
     4801 aacaatatga ggccagtagc taccatattc atcttcatat ttatacttaa gcaaccattc
     4861 cgcaatggtt tcaatcgctt gatattggcc ttttacttca attccagcac tgtaagctac
     4921 agataataaa gccaacggtc caggaatacc gtgtgccatt ccacaattaa aattgcccag
     4981 aggatagtta cgtttatccc ggtctaaaaa ttgaaacttt tgcggggagt accaacctgg
     5041 gacataacga ttgtgcatat gtatgggatg tgtaagatcg gtaagaaatt gaagaattgg
     5101 ctcaacaaga ggagtacaga taggatcggt tgagttcatc aaaatatatc ttccaatccc
     5161 tgaagctccc gaaatggtgt caaaggcttg catgggcaca ccttcgtgaa ggcttaattg
     5221 ttctgtgttc gactgaagcc attgctctgt tagaggcatc atccattgat gtatttgctg
     5281 taagaaatta ctgtagtatt ttccatgatt agaagccact cttacggacg aagcgatgcc
     5341 tgtccagcca ctgtaaagag agagatcgtg aaggccattt ttagcaagtg aggattgaat
     5401 ctgagccatg tttgaatgta tcgctctatt ccaatcatac gaagggtctg tagcacgcca
     5461 ttgcgatagt aaatgaatgt taccaggata accatgcgaa agtgaagttt ctccccatgg
     5521 gtggtacttt gtttctggcg aaacattgtg ttctgcatgg actgctgcat agacggattc
     5581 gggctcgcat agacgtaaag caattttttt actaatatct cttgcacgaa cttggtcgga
     5641 ttgttgtata acaaaactat caattttcat atgaaagcct ccatattttt ttcttcatta
     5701 tattgtcact aaaatctagt gtgttgtcct taaaaatact aaaaaataat ggaggtttat
     5761 cttaaatgta ctcgttgaaa ttaagataaa tgtaagaatt atataaagtc tagttaagat
     5821 tatataactt ataataaaat tgtaaaaaat aaaaggaggt aacaagatga aaaatcaatt
     5881 tgatctggac ttgcaagtag ctaaaaatga ggttgcttca aaaggggttc aaccagccag
     5941 tggtcttatc tgtactcctt catgtgcaac aggtactttg aattgtcaag ttagtcttag
     6001 cttctgcaaa acgtgttaat aacgaaaata catggaaaaa aggcacggtt tctataaccg
     6061 tgtcttttcc agttatgaaa atgagggaag gatttatggt tgaagatttg aaggggagat
     6121 cagttgaaat cagtaagtat atggcacaaa agctaataga cctatcatat acagaaaaga
     6181 tcgttaatca tacaaacaac ctttcacctt cacgtggaaa tcccccttgg aatcctggct
     6241 cgcttgccga tggaattccg ggtgtgtttg cgctgttgtc cgaatggggt tacctggagc
     6301 cagatcaaaa ttgggcagag catgtgcata gtcagttgcg gtacttagat gctaactctc
     6361 tattattatt gcctcgtatt tctctttttt atggaacaac aggagtagct tatggcatat
     6421 tattagcttc caataacagg gctaattata acaaactaat ctctcagctt aataagtata
     6481 ttgaagtaaa gtggggggat ttacctgtaa ttggcacaat tacagagttc agtaggcaaa
     6541 tggatatcat caatgggctg ctcggggtgg gcagatacac tctggaggtt gctgaagaca
     6601 agacgatgtg tgatctgtct cgtcaattat tagacaccat tacgcacttt atacctgaat
     6661 atatacattt gtcgtcaagg gaagattcgg cttcaatcca tctaggtttg gcgcatggta
     6721 tcaccggaat acttgcactg cttgtcactg ccctacaaaa aaagatcgaa gttgaaggac
     6781 tacatgaggc tataaaacat atagccgatt ttttgcttat tcaaattcag gaggatcaat
     6841 atggcgggta ttggcctgta aactctcaag cgtcttcaat gagcaagaga gagaaaaaag
     6901 tagggatgag agaaattgaa ggctggtgtc atggaacctt tggaatagct tggacattat
     6961 tcagggcagg aattgagtgc agtaatgaag aatggaaaaa gatagcggaa aaagctatgc
     7021 aatccatatt ttgcttagat gatgagctac taaactcgct atcccctact ttctgtcacg
     7081 ggttggctgg tttgcttcat atggcccata gattctatag cttaaccgat aatatagctg
     7141 cgttacgctt tgccgatcag atagctttaa agttagtaaa tttatatgat gaagagctcc
     7201 catttggata ccaggatttg gaggggacaa ctcatgttca taatgtagga atactgcaag
     7261 gagcaacagg gattgcgtta agtttgctgg actatggaat tgggaaagaa catcttaaat
     7321 attcagggag atggagtgaa ttgtttctaa tttcttaagg gtgctacctt attgctttat
     7381 ctgaagcatt ggttttggag ggattagatg aaacaaaaat ttatggttat atcatctttt
     7441 aggaagatta agcatttttt tgagacttta agcactccag aaaacattcg aatcatgttt
     7501 tatgattcgc tttaccaggc tataccggag attgatgagc agtgtatcgg tgtagctttg
     7561 aatcaggatc atataacgct agaggacttg tcttactggc acagcattaa agaaaaattc
     7621 acggttccat ttatctttct ttcctatcat agtgatgacc aacggcaatt ggatctcctc
     7681 aaacaactgt tttcaatggc atcatctgaa tatgaaatgg aaagagaaca gcaggctatt
     7741 agtttatcac ctaatgtcat ttttgatcct gctggaaagg ttatttggaa gcaagggcat
     7801 gagcatatgc tgactatgat ggaattccgg ttattaatgt atcttcttac tgcaaaagag
     7861 tgtgcttcta cgattgatca cattatagac agagtctggg gagtggatgc ttttactacg
     7921 tctaatacgg tgtatgtgca catccgaaaa ttgagagaga aaatagaaga tgatccaagt
     7981 catccaaaaa ttctggtaac atgtcatgga atcggttaca agttgaatat atttcctaaa
     8041 aacttggagg tggtcagttg tgaagagaca actgcattac actgatgcgc ataaactaca
     8101 taataaagtg agagatcggg atgaaagatg gtttagtgca tgtgattttt ttctaatcag
     8161 agctccatta ctatcgctag accgatattt tcaggtgttt tcggatcaag ctgtggacag
     8221 ccagcttact ttagaggaaa taactggtcg attaaagttg atgtcccaag aagctgttgt
     8281 ccaagaagcc atatcattag cctctccgtc cctaatgcag tttctacatt acttaaatga
     8341 tggccatgct ttggatgata aaggagagct acaaaagtat gagaagacgc tgaacagctt
     8401 ttatcggtat tttattcgga tgactacccg aacgactcca tttgggttat tttcaggagt
     8461 ggggcagggg atttgtggga aaactaccga tctggatatt ggtagctatc ctaagcatct
     8521 gaagagagcc agacccgaca tggaatggat atataaactg attcaaaaaa tagaagctga
     8581 tccggccata cttaatcaac taaaggtctt tctaaacaaa acggttatga tcaaaggaaa
     8641 tcgagccaaa ttaccattcc gacctaagca tggagcagca gaagctgatg attcggcaga
     8701 aatttttaat tccattcggc ttactgacgt tgttcgaatg acgtttgaag ctgcggaaca
     8761 tccaattcgg gtgatggaac tcaaagaaat actattaaag gcattccctg acgctacttt
     8821 ggaaatcata gctgattatt tgcagcaact agtagagcaa gagtttattt tgagcgagct
     8881 tagacctccg ttaatggatc attcaccctt tcattacata ttcgaaaaaa ttaaaaaact
     8941 ggacatttcc gatccttgga ttcacatttt acacgaaatt gatgctttat tacttcaata
     9001 taatcagtgc acgattggag aaggagagaa ggtctaccgg aaattatgtg acaaaatgct
     9061 ggatatcatt tctgtaaaaa cacctgtaca agttgatgtt aaattggata aagaagtaac
     9121 gctgcccaaa cacgtgcttg atgaggtttc attaggggct gaagtcatgt gcaaattgta
     9181 tgcatctccc aaaacggatg aattggctga atatttgaat cgtttcttgg aaaagtacgg
     9241 tgtgtatcag gaggttccta ttcatgaact gtttgatgag gattggggtc tgggtatacc
     9301 ggaaatgtat cgttcaaatg aaaaaaaatc aatgggcatg gaggcattta aacgcaacgc
     9361 tgttcttttt caatgggttt cgaatgccat ttctaatcat gaaatagaac tggcccttac
     9421 agatgaacag ttagagcaac tgatgatttc tgataactac gacacttccc cagaatcaat
     9481 ggagatctat tatagtatat ttgctgattc tcctgaaaaa gttgattctg gagactattc
     9541 gcttttactt ggagccagtg ctggctcgaa tagagcgggt aaaacctttg gacgttttct
     9601 ggatatgttt agtgaggagt ttgtacaaaa attctctgaa atatatgatc atcaccgatg
     9661 ctttcatccc gatgcgttat tgtcagagat ggtttttatg ccggagcagg ggaaaatatc
     9721 aaatatctca ttaagcaaaa atttaatggc atatgagatt gtgtcgggaa ccacctcttc
     9781 taaagatgag gaccatacgc tctgtattga tgatttagtg gtgggtgcta ctcatgatcg
     9841 tttttatgtc aagtccagac gtttgggtaa ggaaataatc cccttgcaaa cacacatgct
     9901 gaattacaag ctttcaccac atatattcag atttctaacg gatattacta ataaagagat
     9961 tggtacgtgg tcttattttg actgggggcc attagagcag tccccttttt tgccaagact
    10021 agtatataaa aagatcatta ttagtgctgc gcagtggaaa ctgagcaagg cttcattgaa
    10081 tatttcagaa tcatgcgagt ccaaagaagg agaagaactt ttccaggtgt ggagacaaca
    10141 ttggaatgta ccgcgctatg tttacctaac gatgggggat aatcgccttt tattagacct
    10201 atgcaacctc ttccatatca aagaattgct acatgaggtg agcaagacac gtgaaggaca
    10261 ggaacttctt cttaccgagt gtttgggtaa tccctttcat accccagtac aggggacggc
    10321 aggaagatat atgggagaat ttgtgtttcc actaaagaag aaaaaaataa aggtgaagcc
    10381 gagtatccct agttcacaaa gccatattgt ttctcaaaac ttcattgctc atactccttc
    10441 cagcgttcat agaggcatat ttccaggagg ggagtggcta taccttaaat tgtacggagt
    10501 caaggaaaga gagactgacc tggtaggctt aagcctaggg catctcttaa aacagccggg
    10561 gtttaaggat tggagtcgaa aattttactt tttgcgatac gttgatcctg aacatcatat
    10621 ccgtttacgt tttcagggag agcccgataa gctatggaca gctggtttgg cgcaattaaa
    10681 tcagtgggca gccgagttac gtgaagaggg gctaataacg aatatggtca tagatacata
    10741 ccatcctgaa ttggagaggt atggaggaca gacacttatg gagctggcag aacaggtttt
    10801 tgacgtggat agtcaatggg tcatcgccta tatgaaggga attcgctcta atgctttcaa
    10861 tctggacata gagattgcgg ctgtgatgaa cattattcat tatctgagtg gttttggttt
    10921 aacgattcag gagcaagtga aatggttaga agagagaatt gattgcaagc ctcatatgaa
    10981 ggcattcagg gatgtcaaaa aaacattgat tactgctgta gaatcgaatt atgaatggat
    11041 tagacaagca gatacgacca cacaggtggc tttcttagcg cttgtagaaa aaagacaggc
    11101 tatgattcag cagtatgctc aagcgataat cctggctaaa aataatcacg aacttaccaa
    11161 tcatcctgat gacattttag gcagtattat tcatatgcat ttgaatcgtc tgcttggtgt
    11221 aaatagggag cgagaaataa aatgtatggc tttagctaag catacagtag ctaacctgtt
    11281 gcataggaaa ggggtaagct atgtcacagt ctaatggaca aaccaatgac ctgatctctt
    11341 tgtcctataa aaatatattt cgtacctttt tattttggcc tcgtatattc aggctgcttt
    11401 gggacacaca tccttccttt ttgattgcta tactcattac gaatttattg aagggtgcaa
    11461 ttcctgctgt gatcctttta actaccagag aattaatcaa cagtgtcgtg gccggagcgt
    11521 cagggggaag ctttcaacct gtttttatag cttttggcgt cttgatcggt gtcaatttgc
    11581 tgtacgatct gatcaatatt ggcgaaagct tttttcgtaa aatgtttcaa agccggctat
    11641 cgaaccaggt aaatatcaga attattgaaa aagcacaaaa gatgtctttg cagtcgtttg
    11701 aggatgcaga tgtgcaaaat atgcttcaac gtgctcaggg agaggcagat ttcagaccgt
    11761 ttgaaatatt tactcaaatc cttgccatta ttagcagtgt tgtaacctta ttttcgacgg
    11821 gtgcaattct catcgcatgg agatggtggg cgttcttatt catttttctc attccatgct
    11881 tatcatttta ttctttttta cgtatcggac aaagagagtt taatgttcac tttcaaagag
    11941 caggaagaca acgtgaatcc tggtatttgt cgtttttagt gacaagagat aatagtttta
    12001 aagaagtgaa aatatttcaa ttgggagact atctgcgaaa aagatacaaa acgatactcg
    12061 aagggtttta tcgcgaggat aaagtgctag cggttcgtag atcctatact tctcttgtat
    12121 ttcagattgt aaatcagggt gcgtgtgcat ttattatttt catggccata caagccactt
    12181 atgttggtca aattttagtg gggaacttgg tgagcttaat tcagggtatc acgttaaccc
    12241 agtcaacatc gcagagcatt gtacaaggga ttttaagctt gtgtcaaaat aatttgtata
    12301 tcaagcagct atttgacttt ttggatatgc cggaagaggc aaaaatacaa aaatcagttc
    12361 agcaattgcc tttgtccgct attcacagta ttgaatttcg gaatgtatcg tttaaatatc
    12421 cgaacacgga ggagaatgcc atttctaatg ttagcttcaa gcttcatcaa ggagagaagc
    12481 tggccattgt agggaaaaat ggatcgggta aatcaacgat tgtgaagtta atgactcatt
    12541 tgtattccga ttttgaagga gaaatcttaa ttaatggaca ctctatacat gaattggata
    12601 aagacagttt tagagaaaaa ataggcgttg tttttcagga ttttgtacat tatgagatgg
    12661 ctgttagaga taatataggc tttggtaatg tggcttccag agaaaatgat gaggctattt
    12721 ggacagctat agataaggcg ggaatcacag atttgatagt cggcctgcct gataaaataa
    12781 atagcttgct tggaaaatgg tttgaagatg gacagcagct atctggggga cagtggcagc
    12841 gtattgcaat agctagggct tttatgagaa atgccgatat atatgttttg gatgaaccga
    12901 gctctttttt ggatccacac gcggagaaag aggtttttca aaagtttgat cagcttattc
    12961 atgagaaaat tggagtgttt atttcacaca gattgtcctc tgcacgtatg gccgacacca
    13021 ttattttaat ggaggagggc agaattgtag aagcaggtag ccatgaacgc ttaatggata
    13081 tagacggagt gtatgcagaa atgttcaggc ttcaagcctc ctcgtatctt tcacaacgag
    13141 aacgtggttt tgaggaagaa aatctgtcgc cctcgttagt aaggacgaca ggtggatcat
    13201 aa
//
