LOCUS       BGC0001736             12888 bp    DNA     linear   CON 27-FEB-2015
DEFINITION  Photorhabdus luminescens subsp. laumondii TTO1 complete genome.
ACCESSION   BGC0001736
VERSION     BGC0001736
KEYWORDS    .
SOURCE      Photorhabdus laumondii subsp. laumondii TTO1
  ORGANISM  Photorhabdus laumondii subsp. laumondii TTO1
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Photorhabdus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 16:10:28
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 3312940
            Orig. end    :: 3325828
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     subregion       1..12888
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="NRPS"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12888
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     regulatory      complement(1..6)
                     /locus_tag="plu2791"
                     /regulatory_class="ribosome_binding_site"
     CDS             complement(2390..3283)
                     /codon_start=1
                     /db_xref="GOA:Q7N3C5"
                     /db_xref="InterPro:IPR000847"
                     /db_xref="InterPro:IPR005119"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="UniProtKB/TrEMBL:Q7N3C5"
                     /function="Unknown, probable transcriptional regulator"
                     /gene_functions="regulatory (smcogs) SMCOG1014:LysR family
                     transcriptional regulator (Score: 185.7; E-value: 1.9e-56)"
                     /gene_kind="regulatory"
                     /locus_tag="plu2792"
                     /note="unnamed protein product; Similar to transcriptional
                     regulator"
                     /protein_id="CAE15166.1"
                     /transl_table=11
                     /translation="MKLRHLRCFVVVAEELHFARAAERLHIEQSPLSRIIKKLEDDLGT
                     MLFVRTTRNTRLTRAGEILLEQTPRIFSVLGQARDSVKAVAAGFHSQLRIALSDGVTPS
                     KLAELLAKCREDEPETDIRLHEVPLAQHMNGLRDNLFDVGFAQSTIVRDGIIAIPAWYD
                     SLVAVIPARHPLLAYKELPLNEFLRYSLIVCGQETHEGFLRQVLSIFQNAKLVPCISEK
                     VASFDLLLTLVAAGYGIGVAGESQINLARHGDVAIRPFAGGIPKLVTYMLRLDSPLTEE
                     LVRFKERVGNLYHNVY"
     gene            complement(2390..3299)
                     /locus_tag="plu2792"
     regulatory      complement(3294..3299)
                     /locus_tag="plu2792"
                     /regulatory_class="ribosome_binding_site"
     regulatory      3956..3961
                     /locus_tag="plu2793"
                     /regulatory_class="ribosome_binding_site"
     gene            order(3956..3961,3967..4671)
                     /locus_tag="plu2793"
     CDS             3967..4671
                     /codon_start=1
                     /db_xref="GOA:Q7N3C4"
                     /db_xref="InterPro:IPR001474"
                     /db_xref="InterPro:IPR020602"
                     /db_xref="UniProtKB/TrEMBL:Q7N3C4"
                     /function="Unknown"
                     /locus_tag="plu2793"
                     /note="unnamed protein product; Some similarities with GTP
                     cyclohydrolase I precursor"
                     /protein_id="CAE15167.1"
                     /transl_table=11
                     /translation="MPTVEPGDLEVATHGFADGISMQLAKKRALHGPNEVLTKTELNLI
                     IDEAAISFGEFLTALGVDWKNDPNSKDTPRRVAKAYVLDLWKGRYKPMGPITTFPADNY
                     DGIVFDGWIALDSMCSHHHQAISGYVHVAYMPSQYGRVLGLSKLNRIVEHFGRRGLIQE
                     RLTAAIHNAVNQVCERNQGVAVLVDASHNCVRCRGVRHQRSRMKTQMFSGAFAEADSPA
                     KQEFYELIRGIA"
     gene            4656..4661
                     /locus_tag="plu2794"
     regulatory      4656..4661
                     /locus_tag="plu2794"
                     /regulatory_class="ribosome_binding_site"
     gene            4668..5252
                     /locus_tag="plu2794"
     CDS             4668..5252
                     /codon_start=1
                     /db_xref="InterPro:IPR007115"
                     /db_xref="UniProtKB/TrEMBL:Q7N3C3"
                     /function="Unknown"
                     /locus_tag="plu2794"
                     /note="unnamed protein product; Some similarities with
                     6-pyruvoyl tetrahydrobiopterin synthase"
                     /protein_id="CAE15168.1"
                     /transl_table=11
                     /translation="MTIQYITRKGSFDSAHRVMNERVKCFNIHGHTYLYELTFSFEQMQ
                     PIGYAIDFKEIKRIGAAWIDEFLDHASILNPKDADFISAVHATRSKFRLMSLNRHGEYC
                     NPTVENIAKEIFLTMEVLFNEWDNLKIHHIRLYETPNCFTDCNAESIHEEERSAFLSQR
                     FEELKAYSQAMGRFEYDSRNVVTSDSTHERL"
     regulatory      5271..5276
                     /locus_tag="plu2795"
                     /regulatory_class="ribosome_binding_site"
     gene            5271..7239
                     /locus_tag="plu2795"
     CDS             5287..7239
                     /codon_start=1
                     /db_xref="GOA:Q7N3C2"
                     /db_xref="InterPro:IPR001017"
                     /db_xref="InterPro:IPR005475"
                     /db_xref="InterPro:IPR005476"
                     /db_xref="InterPro:IPR009014"
                     /db_xref="InterPro:IPR029061"
                     /db_xref="UniProtKB/TrEMBL:Q7N3C2"
                     /function="Unknown"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DXP_synthase_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1110:1-deoxy-D-xylulose-5-phosphate synthase (Score:
                     236; E-value: 7.1e-72)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="plu2795"
                     /note="unnamed protein product; Similar to
                     3-methyl-2-oxobutanoate dehydrogenase (lipoamide). Putative
                     transmembrane protein"
                     /protein_id="CAE15169.1"
                     /sec_met_domain="DXP_synthase_N (E-value: 1.4e-13,
                     bitscore: 39.8, seeds: 174, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.1e-08, bitscore:
                     24.2, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDSDILRTSLLIRETEEALLRLYSTGELHGTVHTCIGQELTGAIV
                     CKFLKKNDWVFSNHRCHGHFLSRTGDVTGLIAEVMGKETGVCGGRGGSQHLCKEGFFSN
                     GIQGGILPVATGAAFAKKLRHDNSISIAFIGDGTLGEGVVYEVLNIAAKWDLPLLIVLE
                     NNQYAQSTHISETLSGSISKRAQAFGMRYTKSDVWNWEHLIAETEELINYTRNQRHPAF
                     LQIDTYRLKAHSKGDDLRNIDEIEHFNTIDPINVILRERAAELETEVAGVQERVNDAIR
                     KAKQARSSPFYGAETQLQSRSSTFHPLPSQGSKIRLSRAINKAFLEIMELDKNILFIGE
                     DVKAPYGGAFKISDGLSDSFPEQVINTPISESAIVGIGCGLAMHGYCPFVEIMFGDFLT
                     LAFDQILNHAAKFRDMYNDQVKVPLVIRTPMGAGRGYGPTHSQTLEKHFMGIPGLTILA
                     INNLIDPAIVYKTLAKQEEGPVLLIENKILYTKSIRNAPLGFTSYASDDPFPAVVVSPL
                     STNVDVVIFGYGGLSDLLVDVAEELFVEHDVIAQVICPLQIYPFSVIPYIKLVSKCKIA
                     IIVEEGQGFAGFGSEVVAQLTEILGKLLPRTIRIYPSSMAIPSSKALENLMIPGKDMLI
                     ERILKAYISEAEPQQ"
     regulatory      7269..7274
                     /locus_tag="plu2796"
                     /regulatory_class="ribosome_binding_site"
     gene            7269..10088
                     /locus_tag="plu2796"
     CDS             7281..10088
                     /NRPS_PKS="Domain: PCP (407-471). E-value: 2.1e-15. Score:
                     48.6. Matches aSDomain: nrpspksdomains_plu2796_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (492-785). E-value:
                     2.3e-60. Score: 196.0. Matches aSDomain:
                     nrpspksdomains_plu2796_Condensation_LCL.1"
                     /codon_start=1
                     /db_xref="GOA:Q7MB23"
                     /db_xref="InterPro:IPR000089"
                     /db_xref="InterPro:IPR001078"
                     /db_xref="InterPro:IPR001242"
                     /db_xref="InterPro:IPR006162"
                     /db_xref="InterPro:IPR009081"
                     /db_xref="InterPro:IPR011053"
                     /db_xref="InterPro:IPR023213"
                     /db_xref="UniProtKB/TrEMBL:Q7MB23"
                     /function="Unknown"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     230.1; E-value: 9.8e-70)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="plu2796"
                     /note="unnamed protein product; C terminal part similar to
                     peptide synthetase. Putative transmembrane protein"
                     /protein_id="CAE15170.1"
                     /sec_met_domain="PP-binding (E-value: 3.9e-13, bitscore:
                     39.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.7e-46, bitscore:
                     147.7, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNTIILEKLSANDETMLISEWLVEEGDYVTVGQMIARVEASKAI
                     VEIHSPMSGYLLKRYQAGDEVAVGNVIAEIVQEPTSTPTKEDPDASMFQVDRSNATARF
                     SKAATAYLEHHRINPSKLGFSGLITLDYLRQHFAVEKHIQKPFTTTLLADPHESDKILS
                     LVENITTTEPIEVSKQAEIRILSDAQNGSLPSSITVQFNSINLREKLRNDTNSSGQIFP
                     YLLWILGITLQDWPQLRSYYEKQRIHRYSTVNIGVAIDMNHGLRVPVIRQINQLALPNV
                     EAAVADYVCRYHENKLSLEDVTGATFTVSDLSGQNVLHFQPMLNKQQCAILGIGGDKDL
                     EGYPMSLTLVFDHRVITGAIAAQFLNDFKRRLLTSNATKLHLSDTEAQQPTPVHNRFSP
                     TNLKLADDISNIWQSQLECQGVNPSDNFFKLGGDSMKAAHTISQVAAHIAVAVPLRLLF
                     ENPTLQKFTQKITQLINETPRNIDDLLPASPDNYATLSLQQERLLKLCADGDPSDYNLF
                     RAYRLIGHLDREALRQALHHLTKRHEILRSRFDWTTASLIGYFEEDADLPIEIIDLSAI
                     TKEGQDEQLMSALQRNSQCIWHAGDWLFHVSLILLKTDEQVLTLCLHHLIADGESIRIL
                     AAELSTFYAHACGDKSVALPKDLQRQYRHYANWQQQYIRDVSVKRQISYWKHKMEGAPS
                     LLALPICNQSEIYQTRCSASEVFQIPQEIGVLEFSRHIGATPYTVLMSAWAIVLSHYSS
                     QQEVVIGTAADNRQPEYGNNLGFFANNWAARIFVEDTQAFTLLTKSIRRITLDAKDNID
                     WSFGTIADALAPYEKHKVRSRIFQVMLIMLPNAKLTLRNIETIPMNLDIGRGREPFDGT
                     VLDMTLWISVEHENIYAKLVYNVDLFQQKTVKSIVRSFTAVLRKACSESSITLLKLKDT
                     CETIE"
     aSDomain        8502..8693
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_plu2796_PCP.1"
                     /evalue="2.10E-15"
                     /label="plu2796_PCP.1"
                     /locus_tag="plu2796"
                     /protein_end="471"
                     /protein_start="407"
                     /score="48.6"
                     /tool="antismash"
                     /translation="ISNIWQSQLECQGVNPSDNFFKLGGDSMKAAHTISQVAAHIAVAV
                     PLRLLFENPTLQKFTQKIT"
     aSDomain        8757..9635
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_plu2796_Condensation_LCL.1"
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.30E-60"
                     /label="plu2796_Condensation_LCL.1"
                     /locus_tag="plu2796"
                     /protein_end="785"
                     /protein_start="492"
                     /score="196.0"
                     /tool="antismash"
                     /translation="ATLSLQQERLLKLCADGDPSDYNLFRAYRLIGHLDREALRQALHH
                     LTKRHEILRSRFDWTTASLIGYFEEDADLPIEIIDLSAITKEGQDEQLMSALQRNSQCI
                     WHAGDWLFHVSLILLKTDEQVLTLCLHHLIADGESIRILAAELSTFYAHACGDKSVALP
                     KDLQRQYRHYANWQQQYIRDVSVKRQISYWKHKMEGAPSLLALPICNQSEIYQTRCSAS
                     EVFQIPQEIGVLEFSRHIGATPYTVLMSAWAIVLSHYSSQQEVVIGTAADNRQPEYGNN
                     LGFFANNWAARI"
     CDS_motif       8820..8927
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_plu2796_0001"
                     /evalue="3.60E-14"
                     /label="C2_LCL_024-062"
                     /locus_tag="plu2796"
                     /protein_end="549"
                     /protein_start="513"
                     /score="45.1"
                     /tool="antismash"
                     /translation="YNLFRAYRLIGHLDREALRQALHHLTKRHEILRSRF"
     CDS_motif       9144..9209
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_plu2796_0002"
                     /evalue="1.60E-07"
                     /label="C3_LCL_132-143"
                     /locus_tag="plu2796"
                     /protein_end="643"
                     /protein_start="621"
                     /score="23.9"
                     /tool="antismash"
                     /translation="LHHLIADGESIRILAAELSTFY"
     CDS_motif       9546..9626
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_plu2796_0003"
                     /evalue="1.80E-04"
                     /label="C5_LCL_267-296"
                     /locus_tag="plu2796"
                     /protein_end="782"
                     /protein_start="755"
                     /score="14.0"
                     /tool="antismash"
                     /translation="EVVIGTAADNRQPEYGNNLGFFANNWA"
     gene            10095..11456
                     /locus_tag="plu2797"
     CDS             10095..11456
                     /codon_start=1
                     /db_xref="InterPro:IPR011990"
                     /db_xref="InterPro:IPR019734"
                     /db_xref="UniProtKB/TrEMBL:Q7N3C1"
                     /function="Unknown"
                     /locus_tag="plu2797"
                     /note="unnamed protein product; Hypothetical protein"
                     /protein_id="CAE15171.1"
                     /transl_table=11
                     /translation="MIPAQELHRLAHEALNRQDFEHCAKYCYTQLRAANEPYCLQDEIT
                     LDCIYMLLFIHDIYNQQCHLPFTEKWTRHDLFSLAETYYTKKISLDTHIKLKVLKGKYL
                     ANTQSSEAAILAIQDAVILAENSCTPYIKALALAELGHKTMKISLKHGRELIRQAHDIL
                     HQSPVSHELSLENQRHIHFISSIHGVCEFDFGNYEAAMKHLSKVYESSLHLGIKFDISI
                     FCNYLAQVHISLGDYETAESLLLSCLEYFIPEKTPHAWNANNRALLGKVYMEWGKYDKA
                     REHLITGWKESRLARSLPLITLVRNYYAEFLLQRESSPSKQNISLAEEILQENISEAHQ
                     AGIYRSEILSLSLMGQISLSRNDFDAAKKYSQSAIDMLIHMGWLPALRAQEIYMNHYTV
                     AISCGDMEQEKYWLSEAYNVVMSIARRILDDTRRLSFLNRVKINEMIVHKYMET"
     gene            11501..12265
                     /locus_tag="plu2798"
     CDS             11501..12265
                     /codon_start=1
                     /db_xref="GOA:Q7N3C0"
                     /db_xref="InterPro:IPR002198"
                     /db_xref="InterPro:IPR002347"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="InterPro:IPR020904"
                     /db_xref="UniProtKB/TrEMBL:Q7N3C0"
                     /function="Unknown"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     162; E-value: 2.9e-49)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="plu2798"
                     /note="unnamed protein product; Similar to pteridine
                     reductase 1 (H region methotrexate resistance protein)"
                     /protein_id="CAE15172.1"
                     /transl_table=11
                     /translation="MKNEHSTSMRKRRVALITGAARRLGAVIAKTLHAEGFNIVVHCNT
                     SQLEANQLVQELNSIRAQSTISVQADLSNLCNCEPIINAAISEWGQLDILVNNASTYYP
                     TEIGSVSHSQWHDLVDINLKIPFFLIQTAMPWLHQVRGNIVNIVDINAKRPLKGYPVYC
                     AAKAGLALLTEALALELAPNIRVNGVAPGTIMWPEKSNKLDDNEKFTTLKKIPLGRIGD
                     GKDIAEAVRFLAISEYITGHIIRIDGGAILSR"
     gene            12346..12888
                     /locus_tag="plu2799"
     CDS             12346..12888
                     /codon_start=1
                     /db_xref="GOA:Q7N3B9"
                     /db_xref="InterPro:IPR000550"
                     /db_xref="UniProtKB/TrEMBL:Q7N3B9"
                     /function="Unknown"
                     /locus_tag="plu2799"
                     /note="unnamed protein product; Some similarities with
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     pyrophosphokinase"
                     /protein_id="CAE15173.1"
                     /transl_table=11
                     /translation="MKHEKVIAAVAIGSNLSQPYYNLLTAENHLLALKESTNFRFSPIY
                     KTAPIDSTGAPDYLNAVVVFETSLTPVVLLTELLSIETLNGRKRSFVNAPRTLDLDLLL
                     YGDKVISTENLIVPHPRFHKRFFVLTPLNDLLPETQHPLLKASINELEQRLQTLYEKEK
                     YISHFDQTFRSVSTNIH"
ORIGIN
        1 tccccttaaa ttagtaaacc caatctgacc ataaaatcgt atccggcaga attcattgcg
       61 tttaatatgc ctttataatt aacaagtaac gcaatgctgt attaagaaac acagagatac
      121 tggtcagaaa aacgctataa atgtaacaca agtttgaaat taatgtgtaa tgtttactga
      181 cctttttaca ttatccatca gattaaatga atgtttggtg tactgttttt gactgatagt
      241 taatagatat gtcattcaat cgactattgc ccgccagtca cttcatacac ttttagctat
      301 gcctcctatc agtgattcac aacaagcagt gattcacaac aagctacata tatgaaaccc
      361 gttactttag cccccacaca agtaaaagaa gttctaccaa tgagccgatt gttaattaaa
      421 tcaacaaccc attaatatca agaaaaaaat tcaaaaataa gatattgtga ttaattttgt
      481 gagcagatta tcagctttaa caaaaaaatc ctcattaatt tagaaaattt cgcatagtga
      541 catttatcac ataagacttt agaaatcaaa aagataaaaa actcacttat cggatcacaa
      601 tgaaatacca acacatatgc tcaatgtagg aaatatcatc ccaataaata acataatatg
      661 caaaaaacta ataaacatct caacctatat ctgacattta tctgtagact tcctcaatca
      721 caagagcaaa tgatatttcc cctcaactat ttgtgttaag ttatcattaa cattaaaaag
      781 tttttaacga ctgttaccta cttctttaat taattataac gaatacattt ttgcctattt
      841 atctattctc tgaatatatt caaatatata tctaaccgtt cccattattc tattctctat
      901 aagatgattg tctaatattt ccacaagcgg attaaaatga aataacaata aagccgagat
      961 aataagatcc ttttttataa aagttcgagc agtactttga ttacctgcca gatagaccaa
     1021 acttttaaca acatttgtag aaaatttgct gaatgagcaa ctgagttcta agcaaaatcg
     1081 ggtacactgc aaaccaaaat cattacaata atattaccac tttaaatttc cctaagaccg
     1141 gatacgacga gcacagatgt cttagataaa tcaaaaaacg caagacttaa ctttgacaat
     1201 ttaccgtgat ggtcagctaa agacagttta actcaaatca cacctatacc ctctggattt
     1261 caaaatggat cgcgacggca agggaggaat ccccggtcac atagcaaact atgtgaccgg
     1321 ggtgagtgag tgcagccaac aaagaggcaa cttgaaagat aacgggtata tatcagttac
     1381 atgattaatc gccatcgcat tagacgataa aaacaacagg cttaacccaa ctacacataa
     1441 ttaaaaccaa cttttacttt acattacatt aacactatgt tttaatttca ttaaaatatt
     1501 atttcatgta aaaacaatat tgtaaaaaat atgaaaaaac ttctctcata tgcaacataa
     1561 aactggatct tttattatct actgctaata ttttcatttg ttatctttta caaaaaaaat
     1621 atgtttatga agttaatatt gtataaatat ctcactttta ttctaaatgc ataatttaaa
     1681 caatatacgt tgaggttatg aatgatgtta agaagaaaaa ggaagaatcc cactgataac
     1741 aatttaccta taagagtcta tcgtgggaga tctaaatatg aatatcaccc tccatcagga
     1801 ggttcaatat ccatttgttg tttaagttca ccattatctg tcgtttggga ggaatatgaa
     1861 aaaattctta ataaggaaaa acaataacta aataataaca tttcaaatat tatacagtaa
     1921 gtactttatt tacagttata aaaccagcgg gagaacaaac tcccgttagc gtttttatct
     1981 aaatacacca acaataaaga tttttacacg acattaaatt aactatccag ttttaacttc
     2041 tcatataccc tatagatttc aagatggatc gcgacggcaa gggaacgaat ccccgggagc
     2101 atagataacg atgtgaccgg ggtgagtgag tgcagccaac aaagaggcaa cttgaaagat
     2161 aaagggtata cctacttatc gtttatcaaa ccaagctgat catcaaattc cttctcaaga
     2221 taggaatctt aagtccccta ctttggttgg catgaaaacg ctcatcggat tattggaaaa
     2281 gaatagccaa atatttgtca aaaattcgca attctctaca aatttagaag attgcgaatt
     2341 gcacttttaa gtcctagcag agttttaaag cccttttccc ctaccaggat caataaacat
     2401 tgtggtacaa atttccaacc cgttctttga accgtacaag ttcttctgtt aacggggagt
     2461 caagcctaag catatacgtc accagtttag gaataccacc agcaaatggt cttatagcaa
     2521 cgtcgccatg ccgagccaaa ttgatctgtg actctcccgc cacaccaatc ccatacccgg
     2581 cagccactaa agttaacaag agatcaaaac tggcaacctt ttcggagata catggaacta
     2641 actttgcatt ttggaatata gacagaactt gtctcagaaa tccctcgtga gtttcctgac
     2701 cacagacaat cagtgaatag cgcaaaaatt cattcagcgg taattcttta tatgccaaaa
     2761 gcggatgcct ggcgggaata accgccacca atgaatcata ccaagccggt attgcaatga
     2821 tgccatctcg cactatcgta gattgagcga atcccacatc aaaaaggtta tcacgtaatc
     2881 cattcatatg ttgagctaaa ggtacctcgt gtaaacgaat atccgtctcc ggctcgtcct
     2941 cacgacattt tgctaacagc tcagctaatt tggatggtgt aacgccatca gataacgcaa
     3001 tacgaagttg actatggaat ccggccgcaa ccgctttcac actatcacga gcctggccga
     3061 gtactgaaaa gatgcgagga gtttgctcca gtaaaatttc ccctgcacgt gtgagccggg
     3121 tattccttgt agtacgtacg aataacatcg taccaagatc gtcttccaat tttttgatta
     3181 ttcttgataa cggtgattgt tctatatgca ggcgttccgc agctctagca aaatgtaatt
     3241 cttctgctac tactacaaaa caccgtaaat gtcgtaactt catatatatg taacctcaat
     3301 ctttcactaa acagacttat gcctattctt tctaaacgct tctttaattt gaatttgttc
     3361 tttcagatca tctaaatacg ttcagcttta gttggtattg tcccgtaggt tagatcaggg
     3421 gtgacatctc ccatattata aatgccgaat tacataaaaa cctcctttga gtaaacatga
     3481 aataaccgat caccaggcaa gatctgccta gttttagaag aaaatagcat ttgatgatct
     3541 taaaatagtt agctatctag ataccccaaa ttcattaatt tatttaatca atatttatca
     3601 aactgtacgt caatacattt acaataacaa taaataaagc tcactctttc cttaactata
     3661 actattccct agaaagctaa ttattaaata tttatataaa aaaccgaaaa aacggccaaa
     3721 cagatccgac tgaatgcatg atttcaccaa taactaagtg gtgggaccag aaaacaagga
     3781 taccaatatc agcttacctg attaagacct aaaaggtctt aaatatgact gcaaaggaca
     3841 tgatacccac cattatttta tttaggattc agatagtaag aagttaaata ccgatccgtt
     3901 aaatggaaat atgcgtttct ccaaacggat cataatgaac catctagtta caataacgag
     3961 gtgcctatgc cgacggtgga gccaggagac ttggaagttg ccacacatgg atttgccgat
     4021 ggaatatcca tgcaattagc aaaaaagcgc gcattacacg gacccaatga ggttctaacc
     4081 aaaacagaac ttaaccttat tatagatgaa gctgctatat cattcggaga attcctgact
     4141 gctcttggcg ttgactggaa aaatgatcct aactcaaaag acacgccgag acgtgttgca
     4201 aaggcttatg ttcttgacct atggaaaggc cgatacaaac caatgggacc catcactaca
     4261 tttccggcag ataactacga tggcattgta ttcgacggct ggatagcgct cgattccatg
     4321 tgctctcacc atcatcaagc aatcagtggc tatgttcacg ttgcctatat gccaagtcaa
     4381 tacgggcgtg ttcttggctt gtcaaagtta aaccgaatag tcgaacattt cggcagacgt
     4441 gggctcatac aagaacggtt aacagcagca atccacaatg cagtaaacca agtatgtgaa
     4501 agaaaccaag gcgtcgcagt attagtcgat gcatcgcaca attgtgtacg ttgcagaggt
     4561 gtacgccacc agagaagccg tatgaagaca caaatgtttt caggtgcatt cgctgaagct
     4621 gattcaccgg caaaacaaga gttctatgaa ttgattagag gtattgcatg acaattcaat
     4681 atattactcg caaaggctct tttgattccg ctcatcgcgt aatgaatgaa agggtcaaat
     4741 gcttcaatat ccacggacac acttatctgt atgaacttac tttctcattt gaacagatgc
     4801 aaccaattgg atacgcgata gatttcaaag agataaagcg cataggtgcc gcatggattg
     4861 atgagtttct ggatcacgcc tcaattctta atcccaagga tgcagacttt atatccgctg
     4921 ttcatgctac acgcagtaaa ttccgactga tgagcttaaa tcgtcatggg gagtactgta
     4981 atccaacagt tgagaatatt gccaaagaaa tattcctcac tatggaggtt ctttttaatg
     5041 aatgggataa cctaaaaatt catcacatca gactctatga aacacccaat tgttttacag
     5101 attgcaatgc cgagtcaatt catgaagaag aacgcagcgc atttctcagt caacggtttg
     5161 aggaactcaa agcttattca caggcaatgg gccggtttga atatgactca agaaatgttg
     5221 tgacatccga ttctactcat gagcgcttat aaagtaacga atcacgatga atgagggttg
     5281 acgaatatgg acagcgatat cttacggaca tctctactaa tacgagaaac ggaagaagca
     5341 ttgttacgtc tttattccac tggagagctc catggaacag tgcatacatg cattgggcaa
     5401 gagttaacag gcgcaatagt atgcaagttt ctgaaaaaaa atgactgggt attctccaat
     5461 catagatgtc acggccactt tctttcgcgg acaggcgatg ttacaggact gatcgcagag
     5521 gtgatgggaa aagagaccgg cgtgtgtgga ggccgtggag gaagtcaaca tctatgcaaa
     5581 gagggatttt tttctaacgg gattcaaggc gggatcttac cggtagcaac cggggcagct
     5641 ttcgccaaga agttacgcca tgataattcc atctcaatag catttatcgg agatggcact
     5701 ctcggagagg gtgtcgtata tgaagtatta aatatcgcag caaagtggga tctaccgcta
     5761 ttgatcgtgt tagaaaacaa tcaatacgca caatctactc atatctcaga gacattatca
     5821 ggcagcataa gcaaacgcgc acaggctttt ggcatgagat atacgaaatc tgatgtttgg
     5881 aattgggaac atctgatagc tgagacagaa gagctgataa attatactag aaaccaaaga
     5941 caccccgctt ttctacaaat cgatacttac cgtctcaaag ctcactccaa aggagacgat
     6001 ctaagaaaca tcgatgaaat cgaacacttt aatactatcg atcccatcaa tgtcattctg
     6061 cgtgaacgtg ctgctgaatt ggagacagaa gtcgccggag ttcaagaaag agtaaatgac
     6121 gcaataagaa aggcaaaaca ggctcgctca tcaccgtttt acggcgctga aacacaattg
     6181 caatcgcgat cttccacatt tcatccgcta cccagccagg ggtctaagat tcggctatcc
     6241 cgtgcaatca ataaggcatt tttggaaatc atggagctgg ataaaaatat cctgttcata
     6301 ggagaagacg tgaaagcgcc ttatggcggc gcatttaaga tatccgatgg cctgtccgat
     6361 agctttcccg aacaagtcat aaatactccc atctccgaaa gcgctattgt cggcatcgga
     6421 tgtgggctcg ccatgcatgg atattgtcct tttgtggaaa tcatgttcgg cgacttcttg
     6481 actttagcgt ttgaccaaat attgaatcac gcagccaagt tcagggacat gtacaacgat
     6541 caggtgaaag ttccattagt gatccggaca ccaatgggcg ctggccgcgg ttacgggcct
     6601 acccatagtc aaactctgga aaagcatttc atgggaatac caggtctgac gatactggca
     6661 attaacaatc ttatcgatcc ggcaatagtc tataaaacac ttgcaaaaca ggaggaaggt
     6721 cctgttcttc ttattgaaaa taaaatcctc tataccaaga gcattcgaaa tgctccattg
     6781 gggttcacaa gctatgcctc tgacgatcca ttcccagcgg tcgttgtaag ccctctgtca
     6841 acaaacgtcg acgttgtaat cttcggttat ggtgggctga gcgatctact ggtagatgta
     6901 gcagaagaac ttttcgtaga acacgatgtg attgctcaag ttatctgccc actccagata
     6961 tatccgttta gtgtaatccc atatatcaaa ctcgtatcga aatgcaaaat cgccattatc
     7021 gttgaagaag gtcagggatt tgcgggtttc ggttcagaag tcgttgctca attgactgaa
     7081 attttgggta agttattacc tcgtacaata cgcatatatc cttccagtat ggcgataccc
     7141 tcaagtaaag cccttgaaaa ccttatgatt ccaggaaaag acatgctgat tgaacgtatt
     7201 cttaaggcat acatatcaga ggctgagcct caacaataaa tgtctttaat tgaaattaat
     7261 acgaccacaa taggtatatt atgaaaaata ccattattct tgagaagcta agcgccaacg
     7321 acgagacaat gttaatctca gaatggttgg tcgaagaggg agattatgtc actgtcgggc
     7381 aaatgatcgc gagggtagaa gcttccaaag ccatagtgga aatccatagc cctatgtcag
     7441 gatatctgct taagaggtat caagcaggag atgaggttgc tgtaggaaat gttatcgctg
     7501 aaatcgtcca agaacctact tccaccccaa ccaaggaaga tcccgacgca tccatgttcc
     7561 aagtggatag aagcaacgca acggccagat tctctaaggc cgctaccgca tatctcgaac
     7621 accacagaat taatccttca aaactgggat tcagcggcct cataactttg gattacctca
     7681 gacaacattt cgctgtggaa aaacacattc aaaagccatt cacaactaca ctattagctg
     7741 acccccacga atcggataag attttatcat tagtcgaaaa tatcacgact acggaaccga
     7801 tcgaagtttc gaaacaagct gaaatacgca ttctctctga cgctcagaat ggctcattac
     7861 ccagttctat caccgtgcag ttcaattcca tcaatctacg ggaaaaattg cgaaatgata
     7921 caaattccag tggacaaatt ttcccttatc ttttgtggat actcggtatt acactgcaag
     7981 actggccaca attacgctct tattatgaga aacaacggat tcatcgctac agcacagtga
     8041 atattggcgt tgcaattgac atgaatcatg gcctgcgagt ccccgtgatt cggcaaataa
     8101 atcaattagc attgccaaat gtggaagccg ctgtcgccga ttatgtctgc cgataccatg
     8161 aaaacaaatt aagccttgaa gatgttacgg gagcgacctt taccgtttcc gacctgtctg
     8221 gtcagaatgt tcttcatttt cagcccatgt taaataagca gcaatgcgcc atcctaggaa
     8281 tcggaggtga taaagatctt gagggatacc ccatgtcact caccttagta ttcgatcata
     8341 gagtcatcac tggtgccatc gcagcacaat ttttaaatga ctttaaacga agactactca
     8401 ccagcaatgc cactaaatta cacttatccg atacagaagc tcagcaaccg accccggttc
     8461 acaatcgctt ttccccgaca aatttaaaat tagctgacga tatcagcaat atttggcaat
     8521 cgcagcttga atgccaagga gtgaatccat cagacaattt cttcaaactc ggcggtgact
     8581 cgatgaaggc tgcccacacg atatcgcaag tcgcggctca tattgccgtt gctgtacctc
     8641 tgcgattgct tttcgaaaac cccacccttc aaaaattcac ccagaaaatt acacagctga
     8701 ttaatgaaac accacgaaac attgacgatc ttttacctgc gtcgccggat aactacgcca
     8761 cactctctct tcaacaagag agattactca aattatgcgc tgatggagat ccctctgact
     8821 ataacctttt ccgcgcttat cggctcattg gtcatctcga tagagaggca ttaaggcaag
     8881 cactgcatca cctaacaaaa cgacacgaaa tccttcgtag ccgattcgat tggaccaccg
     8941 cttcattgat tgggtacttt gaagaagatg ccgatctgcc aattgagata attgaccttt
     9001 ctgcaataac caaagaaggt caagacgaac aattaatgtc cgccttacaa cgtaattcac
     9061 aatgtatctg gcatgcaggc gactggctgt ttcacgtaag cctgattctt ctcaaaacag
     9121 acgagcaggt tttaacactt tgtctacatc acttgattgc agatggtgag tctattcgaa
     9181 ttctcgctgc cgaattatca actttctatg cccatgcatg cggtgacaaa tcagtcgctt
     9241 tgccaaaaga tctacaacga caatatcgcc actatgctaa ctggcaacaa caatatatcc
     9301 gtgatgtatc tgtaaaacgt caaataagtt actggaaaca taaaatggaa ggtgcacctt
     9361 ccctactcgc tctccctata tgcaatcaat cagaaatcta tcaaacaaga tgttccgcat
     9421 cagaagtgtt tcagatccca caagagattg gtgtccttga attcagccgc catataggag
     9481 ccacacccta tacggttctt atgtcagctt gggcaattgt tctttcccat tattcatccc
     9541 aacaagaagt cgttattggc actgcggcgg acaacagaca accggaatat ggaaataatc
     9601 tcggtttctt tgcaaacaat tgggctgcac gcatattcgt tgaagataca caagcattca
     9661 cattactgac taaatcgata cgccgaatca ctctggacgc aaaggataat attgattggt
     9721 ctttcggcac aattgccgat gcactcgctc cttatgaaaa acacaaagta cgatcacgca
     9781 tctttcaggt catgttaatc atgcttccca acgcaaaatt gaccttacga aacatcgaga
     9841 ccatacccat gaacttagat atagggcgtg gcagagaacc ttttgacggt acagttttag
     9901 atatgacgct atggatatct gtagagcatg aaaatatata tgcaaagcta gtatataacg
     9961 ttgatctctt tcaacagaaa accgtgaaat ccatagttcg atcattcaca gcagtccttc
    10021 ggaaagcatg ttcggagagc agtattacgc tgcttaaact taaagatact tgtgaaacca
    10081 ttgagtaatc aagcatgata ccagctcaag aattacaccg attagcacac gaagcattaa
    10141 atcgtcaaga tttcgaacac tgcgccaaat attgttacac acagctcagg gctgcaaatg
    10201 aaccgtattg tctgcaagat gaaatcactc tagattgtat ttatatgcta ctgtttatac
    10261 atgacattta taatcaacaa tgccatctcc ctttcactga gaaatggaca agacatgatc
    10321 tattctcctt ggcagaaact tattatacta agaaaatatc acttgatacg catattaaac
    10381 tgaaagttct caaaggaaag tacctcgcca atacacaatc atcggaagca gccatcttgg
    10441 caatacaaga tgcagtcatt ttagctgaaa acagttgtac tccctatatc aaagcccttg
    10501 cattagctga attaggccac aagactatga aaatcagcct taagcatggg agagaattga
    10561 tccggcaagc ccacgatatc ctgcatcaga gtccagtaag tcatgaactg tcattggaaa
    10621 accaaaggca tatccatttt atttcttcga tacatggagt ttgcgaattc gattttggaa
    10681 actacgaagc agcaatgaaa catctgtcca aagtttacga gtcaagcctg cacctcggca
    10741 tcaaattcga catttccatc ttctgcaact acctggcaca agtccatatc agtctaggag
    10801 attatgaaac cgctgagtcg cttctactca gctgccttga atatttcatc cctgagaaaa
    10861 cgccacacgc atggaatgca aataatcgtg cactccttgg caaggtctat atggaatggg
    10921 gaaaatatga caaggcacgt gaacacctca taacagggtg gaaagagagc agattagctc
    10981 gcagcttacc gttgataacg cttgtcagaa attactatgc agaatttctt ttacaacgag
    11041 aaagtagtcc gtccaaacaa aatatctcct tggccgaaga aatcttgcag gaaaatatct
    11101 ctgaagcaca ccaagcggga atatatcgta gcgaaatcct ttctttatcg cttatgggac
    11161 aaatttccct cagccgaaat gactttgacg ccgccaaaaa atatagccaa tctgctattg
    11221 atatgctcat tcacatggga tggctacccg ctctccgcgc acaggagatc tacatgaacc
    11281 actataccgt agcaatcagc tgtggagata tggagcagga aaaatactgg ttatcggaag
    11341 cttataatgt ggtaatgagt atagctcgtc gtattcttga tgataccaga cgcctttcat
    11401 ttttaaatcg ggtaaaaatc aatgaaatga ttgttcacaa atatatggaa acttaattta
    11461 tctgtattcg tgagatctac tttaatgacg gaaaatcaat atgaaaaatg aacattctac
    11521 atcaatgcga aagcgacgag ttgcactgat cacgggtgct gccagaagat taggcgcggt
    11581 tatcgcaaaa acattgcatg ctgagggatt caatatagtg gtacactgta atacatccca
    11641 attagaggcc aaccaactcg ttcaggaatt aaattcgata cgagcccaat ctacaatatc
    11701 cgtgcaagcc gatctgagta atttatgcaa ttgtgaaccc atcataaatg cagcaataag
    11761 cgaatgggga caattggata tattagtcaa taatgcctca acctattacc caacagaaat
    11821 tggatctgtt tcccatagcc aatggcatga tttggtcgat attaatctaa aaataccgtt
    11881 ttttctgatc caaactgcca tgccttggct gcatcaggtt cgtgggaaca tagtcaatat
    11941 tgtagatatc aatgctaagc gtcctctaaa aggatatcct gtctattgtg ctgcaaaagc
    12001 gggcctcgca ctactcacag aagccctcgc tttagagtta gcacccaata tccgggtgaa
    12061 tggcgttgct ccgggaacga tcatgtggcc tgaaaaaagt aataaattgg acgataacga
    12121 aaaattcacc actttgaaaa aaatccccct tggccgaatt ggtgatggca aagacatcgc
    12181 tgaggcggta cgatttcttg caatttcaga gtatattaca ggtcacatca tcaggatcga
    12241 tggcggtgca atactttcca gataaaagtt ccacgctagc aacatacggt ttcatgatgg
    12301 aataataagt gtcaggtgat ttaatttttt gcgagtaaat atgatatgaa gcacgaaaaa
    12361 gtcatagcag cagtagcaat tggaagtaat cttagtcagc cttattacaa cttgttaaca
    12421 gcagaaaatc atttgctggc attgaaggaa agtacaaact tccgatttag tccgatatat
    12481 aaaacagcac cgattgacag tacaggagca ccagactact tgaatgccgt tgttgtcttt
    12541 gaaacatcgc taacaccggt cgttttgctc accgaattgc taagtattga aactctcaac
    12601 ggcaggaaac gctcgtttgt caatgctcct cgaacgctgg atcttgattt actcttatac
    12661 ggagacaagg ttatatctac ggagaacctg attgttcctc atccgaggtt tcataagcgc
    12721 ttctttgttt tgacaccact caatgactta ttacctgaaa cacaacatcc actccttaaa
    12781 gcatcgatta atgaacttga gcaaagattg caaacactgt atgaaaaaga gaaatatata
    12841 tctcatttcg accaaacatt tcgatctgtt tctactaaca ttcattag
//
