BGC0001737 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001737 (Corallococcus coralloides)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T1PKS,NRPS	1	53,488	High	phenalamide	NRPS:Type I+PKS

BGC0001737 - Region 1 - NRPS,T1PKS

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Location: 1 - 53,488 nt. (total: 53,488 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 53,489
Label		BGC0001737

Tailoring functions

Oxidoreductases: dehydrogenase

phaC: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase

phaB: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

phaF: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Ligases: ligase synthase

phaA: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

phaD: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

phaE: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

phaG: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

phaI: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001737.3	1.27	NRPS, PKS	phenalamide	Corallococcus coralloides
BGC0001273.3	0.81	PKS	asperlactone	Aspergillus ochraceus
BGC0001394.5	0.80	NRPS, PKS	phenalamide A2	Myxococcus stipitatus DSM 14675
BGC0001276.3	0.79	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.79	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.79	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15
BGC0001268.4	0.67	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0002192.3	0.67	PKS	FR901512	fungal sp. No.14919
BGC0002191.2	0.66	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001737.3	1.00	NRPS, PKS	phenalamide	Corallococcus coralloides
BGC0001394.5	0.85	NRPS, PKS	phenalamide A2	Myxococcus stipitatus DSM 14675
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.76	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.76	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.76	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001284.4	0.67	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002191.2	0.67	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001268.4	0.67	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001022.5	0.67	NRPS, PKS	myxalamid, myxalamid A, myxalamid C, myxalamid D	Stigmatella aurantiaca

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 53488:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-redmal) + (Me-ccmal) + (Me-ccmal) + (Me-ccmal) + (pk - pk - ccmal) + (Me-redmal) + (Ala)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

phaG: mmal

PKS_AT (533..829): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 197.6
Ethylmalonyl-CoA: 178.4
Methoxymalonyl-CoA: 154.0
Isobutyryl-CoA: 134.4
Benzoyl-CoA: 124.2
Malonyl-CoA: 112.5
2-Methylbutyryl-CoA: 103.3
Acetyl-CoA: 91.2
Propionyl-CoA: 90.1
trans-1,2-CPDA: 89.5
CHC-CoA: 72.8
3-Methylbutyryl-CoA: 51.6
fatty_acid: 36.1
inactive: 29.2

phaF: mmal

PKS_AT (558..853): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 87.5%; Malonyl-CoA: 75.0%; Methoxymalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 194.9
Ethylmalonyl-CoA: 179.3
Methoxymalonyl-CoA: 151.6
Isobutyryl-CoA: 114.3
Malonyl-CoA: 103.5
Benzoyl-CoA: 94.7
2-Methylbutyryl-CoA: 92.9
trans-1,2-CPDA: 92.4
Propionyl-CoA: 89.6
CHC-CoA: 76.0
fatty_acid: 65.6
Acetyl-CoA: 65.6
3-Methylbutyryl-CoA: 40.5
inactive: 22.5

phaE: mmal

PKS_AT (560..856): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.4
Ethylmalonyl-CoA: 151.6
Methoxymalonyl-CoA: 139.7
Malonyl-CoA: 108.9
Isobutyryl-CoA: 108.1
Benzoyl-CoA: 89.7
trans-1,2-CPDA: 85.7
2-Methylbutyryl-CoA: 81.8
Acetyl-CoA: 70.6
Propionyl-CoA: 63.2
CHC-CoA: 57.5
3-Methylbutyryl-CoA: 51.4
inactive: 24.8
fatty_acid: 19.8

phaD: mmal

PKS_AT (567..863): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 181.1
Ethylmalonyl-CoA: 163.5
Methoxymalonyl-CoA: 138.0
Malonyl-CoA: 104.6
Isobutyryl-CoA: 90.6
trans-1,2-CPDA: 86.4
Benzoyl-CoA: 86.2
Acetyl-CoA: 77.3
2-Methylbutyryl-CoA: 74.0
Propionyl-CoA: 58.2
CHC-CoA: 55.0
3-Methylbutyryl-CoA: 47.6
inactive: 37.5
fatty_acid: 20.3

phaC: pk - pk - mal

PKS_AT (561..859): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; Methylmalonyl-CoA: 83.3%; Ethylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 144.1
Malonyl-CoA: 128.2
Ethylmalonyl-CoA: 122.9
Methoxymalonyl-CoA: 109.7
Acetyl-CoA: 94.4
Isobutyryl-CoA: 86.9
2-Methylbutyryl-CoA: 83.1
Benzoyl-CoA: 80.2
Propionyl-CoA: 61.4
trans-1,2-CPDA: 56.6
CHC-CoA: 37.6
inactive: 35.2
3-Methylbutyryl-CoA: 33.3
fatty_acid: 28.5

PKS_AT (2370..2666): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 141.6
Methoxymalonyl-CoA: 129.0
Malonyl-CoA: 125.6
Isobutyryl-CoA: 122.5
Ethylmalonyl-CoA: 108.1
Acetyl-CoA: 77.0
Propionyl-CoA: 70.5
2-Methylbutyryl-CoA: 70.4
Benzoyl-CoA: 68.8
3-Methylbutyryl-CoA: 67.0
trans-1,2-CPDA: 62.2
inactive: 54.5
CHC-CoA: 50.0
fatty_acid: 35.0

PKS_AT (3890..4183): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 212.1
inactive: 165.7
Methoxymalonyl-CoA: 87.2
Methylmalonyl-CoA: 80.8
Propionyl-CoA: 70.1
Ethylmalonyl-CoA: 55.8
2-Methylbutyryl-CoA: 48.8
Isobutyryl-CoA: 41.1
CHC-CoA: 37.7
Benzoyl-CoA: 33.7
trans-1,2-CPDA: 30.3
Acetyl-CoA: 26.2
3-Methylbutyryl-CoA: 20.1
fatty_acid: 12.3

phaB: mmal

PKS_AT (569..867): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.2
Ethylmalonyl-CoA: 158.6
Methoxymalonyl-CoA: 144.1
Malonyl-CoA: 107.7
Isobutyryl-CoA: 100.9
2-Methylbutyryl-CoA: 100.4
trans-1,2-CPDA: 91.8
Propionyl-CoA: 85.8
Benzoyl-CoA: 84.5
CHC-CoA: 79.1
Acetyl-CoA: 72.1
fatty_acid: 49.3
3-Methylbutyryl-CoA: 39.8
inactive: 26.2

phaA: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (541..931): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor