BGC0001741 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001741 (Phyllosticta cirsii)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS,T1PKS	1	42,946	High	phyllostictine A/phyllostictine B	NRPS:Type I+PKS

BGC0001741 - Region 1 - NRPS,T1PKS

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Location: 1 - 42,946 nt. (total: 42,946 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 42,947
Label		BGC0001741

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxygenase

phyL1: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

phyL4: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

phyL5: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

phyL6: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

Transferases: methyltransferase

phyL7: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Ligases: synthase

phyS: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001741.3	2.00	NRPS, PKS	phyllostictine A, phyllostictine B	Phyllosticta cirsii
BGC0002192.3	0.95	PKS	FR901512	fungal sp. No.14919
BGC0001268.4	0.94	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.90	PKS	ACT-Toxin II	Alternaria alternata
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002230.2	0.48	NRPS, PKS	tolypyridone C	Tolypocladium sp. 49Y
BGC0002035.3	0.46	NRPS, PKS	ilicicolin H, ilicicolin J, 8-epi-ilicicolin H	Neonectria sp. DH2
BGC0002538.3	0.45	PKS	chaetolivacine A, chaetolivacine B, chaetolivacine C	Chaetomium olivaceum
BGC0002505.3	0.44	NRPS, PKS	xenoacremone A	Xenoacremonium sp. BF-2018a
BGC0001305.4	0.43	PKS	fujikurin A, fujikurin B, fujikurin C, fujikurin D	Fusarium fujikuroi IMI 58289

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001741.3	1.00	NRPS, PKS	phyllostictine A, phyllostictine B	Phyllosticta cirsii
BGC0002192.3	0.77	PKS	FR901512	fungal sp. No.14919
BGC0001268.4	0.77	NRPS, PKS	fusarin C	Fusarium fujikuroi
BGC0001254.3	0.76	PKS	ACT-Toxin II	Alternaria alternata
BGC0002191.2	0.65	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002230.2	0.57	NRPS, PKS	tolypyridone C	Tolypocladium sp. 49Y
BGC0002035.3	0.56	NRPS, PKS	ilicicolin H, ilicicolin J, 8-epi-ilicicolin H	Neonectria sp. DH2
BGC0002538.3	0.56	PKS	chaetolivacine A, chaetolivacine B, chaetolivacine C	Chaetomium olivaceum
BGC0002505.3	0.55	NRPS, PKS	xenoacremone A	Xenoacremonium sp. BF-2018a
BGC0001305.4	0.54	PKS	fujikurin A, fujikurin B, fujikurin C, fujikurin D	Fusarium fujikuroi IMI 58289

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 4681 - 42946:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-pk - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

phyS: pk - X

PKS_AT (548..873): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 58.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 82.3
Methoxymalonyl-CoA: 68.1
Malonyl-CoA: 41.5
Isobutyryl-CoA: 38.3
fatty_acid: 35.1
trans-1,2-CPDA: 31.6
Benzoyl-CoA: 25.2
Propionyl-CoA: 23.1
CHC-CoA: 15.2
3-Methylbutyryl-CoA: 8.9
Acetyl-CoA: 4.5
2-Methylbutyryl-CoA: 4.3
inactive: 0.0
Ethylmalonyl-CoA: -1.1

AMP-binding (3081..3482): X

nrpys: Orn, Lys, Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DFDKFGSIAK
Nearest Stachelhaus code(s):: Phe NHDKFGSISK (76% 8Å match); MalAc NHDKFGSISK (74% 8Å match); Ser DFDTFGGILK (59% 8Å match); Tyr DFESFGNIAK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

Link to PARAS substrate predictor