BGC0001750 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001750 (Nannocystis pusilla)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS	1	38,255	pyrronazol B	NRPS:Type I+PKS	100%

BGC0001750 - Region 1 - NRPS,T1PKS

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Location: 1 - 38,255 nt. (total: 38,255 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 38,256
Label		BGC0001750

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001750	1.98	NRP, Polyketide	pyrronazol B	Nannocystis pusilla
BGC0000996	0.66	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0002608	0.65	NRP, Polyketide	ochratoxin A	Aspergillus steynii
BGC0002084	0.64	RiPP	biarylitide YYH, biarylitide YFH	Planomonospora sp. ID82291
BGC0002341	0.64	Other	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002217	0.64	Terpene	HEx-tc3 terpene	Botrytis cinerea T4
BGC0001273	0.63	Polyketide	asperlactone	Aspergillus ochraceus
BGC0000343	0.62	NRP	enterobactin	Pseudomonas sp. J465
BGC0001275	0.62	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001068	0.62	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001750	1.00	NRP, Polyketide	pyrronazol B	Nannocystis pusilla
BGC0000996	0.66	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0002608	0.66	NRP, Polyketide	ochratoxin A	Aspergillus steynii
BGC0002084	0.65	RiPP	biarylitide YYH, biarylitide YFH	Planomonospora sp. ID82291
BGC0002341	0.65	Other	3,5-dibromo-p-anisic acid	Planctomycetales bacterium 10988
BGC0002217	0.65	Terpene	HEx-tc3 terpene	Botrytis cinerea T4
BGC0001273	0.65	Polyketide	asperlactone	Aspergillus ochraceus
BGC0000343	0.65	NRP	enterobactin	Pseudomonas sp. J465
BGC0001275	0.64	Polyketide	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001068	0.64	Terpene, Polyketide	pyripyropene A	unidentified unclassified sequences.

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 38255:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (Ser) + (Me-ccmal) + (Me-mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

pynC

Search NORINE for peptide: strict or relaxed

AMP-binding (9..400): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLLYLALVCK
Nearest Stachelhaus code(s):: Pro DLLYLALVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

pynG

PKS_AT (587..889): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 165.7
Ethylmalonyl-CoA: 148.5
Methoxymalonyl-CoA: 134.6
Isobutyryl-CoA: 94.5
2-Methylbutyryl-CoA: 91.7
Malonyl-CoA: 91.3
trans-1,2-CPDA: 88.1
CHC-CoA: 82.6
Benzoyl-CoA: 80.3
Propionyl-CoA: 71.3
Acetyl-CoA: 59.4
fatty_acid: 37.4
3-Methylbutyryl-CoA: 34.7
inactive: 31.2

pynH

Search NORINE for peptide: strict or relaxed

AMP-binding (537..938): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLWNIASIWK
Nearest Stachelhaus code(s):: Ser DLWNIASIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

pynI

PKS_AT (600..888): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 164.9
Ethylmalonyl-CoA: 154.5
Methoxymalonyl-CoA: 134.1
Malonyl-CoA: 100.2
Benzoyl-CoA: 92.0
Isobutyryl-CoA: 84.4
trans-1,2-CPDA: 77.2
2-Methylbutyryl-CoA: 76.0
Propionyl-CoA: 74.4
Acetyl-CoA: 66.0
CHC-CoA: 52.3
3-Methylbutyryl-CoA: 38.9
fatty_acid: 30.9
inactive: 27.6

pynJ

PKS_AT (673..965): mmal

ATSignature: Methylmalonyl-CoA, Ethylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 70.8%; Ethylmalonyl-CoA: 70.8%; Malonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 127.1
Malonyl-CoA: 112.0
Methoxymalonyl-CoA: 109.2
Ethylmalonyl-CoA: 105.1
Isobutyryl-CoA: 78.6
trans-1,2-CPDA: 76.6
2-Methylbutyryl-CoA: 73.6
Propionyl-CoA: 68.1
fatty_acid: 52.5
CHC-CoA: 48.5
Benzoyl-CoA: 46.6
inactive: 40.1
Acetyl-CoA: 29.7
3-Methylbutyryl-CoA: 19.9