BGC0001763: rufomycin biosynthetic gene cluster from Streptomyces atratus
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Location: 1 - 55,642 nt. (total: 55,642 nt).
This entry is originally from NCBI GenBank LC257593.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001763
Short description rufomycin biosynthetic gene cluster from Streptomyces atratus
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: unknown
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Unknown)
Loci
LC257593.1
via
Compounds
  • rufomycin
Species Streptomyces atratus [taxonomy]
References
Chemical products information
rufomycin Evidence:
Copy SMILES
C54H77N9O10
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • BBA20948.1
  • rufA
 543 - 1310 (+) LacI-like regulatory protein
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    • BBA20949.1
    • rufB
    		 1384 - 2148 (-) alpha/beta hydrolase
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      • BBA20950.1
      • rufC
      		 2470 - 3531 (+) cytochrome P450
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        • BBA20951.1
        • rufD
        		 3536 - 3682 (-) hypothetical protein
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          • BBA20952.1
          • rufE
          		 3671 - 18142 (+) type I polyketide synthase
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            • BBA20953.1
            • rufF
            		 18159 - 18875 (+) thioesterase
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              • BBA20954.1
              • rufG
              		 19152 - 19253 (+) hypothetical protein
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                • BBA20955.1
                • rufH
                		 19488 - 19649 (+) MbtH-like protein
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                  • BBA20956.1
                  • rufI
                  		 19646 - 20791 (+) aminotransferase
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                    • BBA20957.1
                    • rufJ
                    		 20947 - 21354 (+) hypothetical protein
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                      • BBA20958.1
                      • rufK
                      		 21362 - 22417 (+) ABC-transporter
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                        • BBA20959.1
                        • rufL
                        		 22414 - 23247 (+) ABC-transporter
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                          • BBA20960.1
                          • rufM
                          		 23505 - 24590 (+) cytochrome P450
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                            • BBA20961.1
                            • rufN
                            		 25015 - 26151 (+) nitric oxide synthase
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                              • BBA20962.1
                              • rufO
                              		 26129 - 27313 (+) cytochrome P450
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                                • BBA20963.1
                                • rufP
                                		 27335 - 28456 (+) tryptophan N-dimethylallyltransferase
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                                  • BBA20964.1
                                  • rufQ
                                  		 28637 - 29011 (+) hypothetical protein
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                                    • BBA20965.1
                                    • rufR
                                    		 29042 - 29770 (-) luciferase-like monooxygenase
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                                      • BBA20966.1
                                      • rufS
                                      		 30144 - 31343 (+) cytochrome P450
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                                        • BBA20967.1
                                        • rufT
                                        		 31567 - 55542 (+) nonribosomal peptide synthetase
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                                          Biosynthesis information

                                          Biosynthetic modules

                                          Name
                                          Unk01
                                          Type
                                          nrps-type1
                                          Genes
                                          rufT
                                          Substrates
                                          1-(1,1-dimethylallyl)-tryptophan (evidence: Sequence-based prediction [1])
                                          Integrated Monomers
                                          Domains
                                          adenylation
                                          Name
                                          Unk02
                                          Type
                                          nrps-type1
                                          Genes
                                          rufT
                                          Substrates
                                          leucine (evidence: Sequence-based prediction [1])
                                          Integrated Monomers
                                          Domains
                                          adenylation
                                          Name
                                          Unk03
                                          Type
                                          nrps-type1
                                          Genes
                                          rufT
                                          Substrates
                                          3-nitrotyrosine (evidence: Sequence-based prediction, Knock-out studies, Mass spectrometry)
                                          Integrated Monomers
                                          Domains
                                          adenylation
                                          Name
                                          Unk04
                                          Type
                                          nrps-type1
                                          Genes
                                          rufT
                                          Substrates
                                          alanine (evidence: Sequence-based prediction [1])
                                          Integrated Monomers
                                          Domains
                                          adenylation
                                          Name
                                          Unk05
                                          Type
                                          nrps-type1
                                          Genes
                                          rufT
                                          Substrates
                                          leucine (evidence: Sequence-based prediction [1])
                                          Integrated Monomers
                                          Domains
                                          adenylation
                                          Name
                                          Unk06
                                          Type
                                          nrps-type1
                                          Genes
                                          rufT
                                          Substrates
                                          leucine (evidence: Sequence-based prediction [1])
                                          Integrated Monomers
                                          Domains
                                          adenylation
                                          Name
                                          Unk07
                                          Type
                                          nrps-type1
                                          Genes
                                          rufT
                                          Substrates
                                          trans-2-crotylglycine (evidence: Sequence-based prediction [1])
                                          Integrated Monomers
                                          Domains
                                          adenylation
                                          Annotation changelog

                                          Entry version: 4

                                          Date
                                          Changes
                                          Submitters
                                          Reviewers
                                          2022-10-07
                                          Update chemical activity to schema version 2.11
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                          Entry version: 3

                                          Date
                                          Changes
                                          Submitters
                                          Reviewers
                                          2022-09-15
                                          Updated bioactivity data
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          2022-09-15
                                          Added NRP substrate specificities
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                          Entry version: 2

                                          Date
                                          Changes
                                          Submitters
                                          Reviewers
                                          2019-10-16
                                          Migrated from v1.4
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                          Entry version: 1

                                          Date
                                          Changes
                                          Submitters
                                          Reviewers
                                          2018-08-06
                                          Submitted
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                          Detailed domain annotation
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