BGC0001770 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001770 (Streptomyces sp. SD85)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS	1	94,427	sceliphrolactam	PKS	100%

BGC0001770 - Region 1 - T1PKS

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Location: 1 - 94,427 nt. (total: 94,427 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 94,428
Label		BGC0001770

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001770	1.00	Polyketide	sceliphrolactam	Streptomyces sp. SD85
BGC0002517	0.47	Polyketide	tripartilactam, niizalactam C	Streptomyces sp.
BGC0000844	0.35	Other	clavulanic acid	Streptomyces clavuligerus
BGC0001164	0.34	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001163	0.33	Polyketide	1-heptadecene	Moorea producens 3L
BGC0002631	0.32	RiPP	myxarylin	Pyxidicoccus fallax
BGC0000122	0.32	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0002000	0.32	Polyketide	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000892	0.31	Other	caryoynencin	Burkholderia gladioli BSR3
BGC0002514	0.31	Polyketide	julichrome Q6.6	Streptomyces sampsonii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001770	1.00	Polyketide	sceliphrolactam	Streptomyces sp. SD85
BGC0002517	0.77	Polyketide	tripartilactam, niizalactam C	Streptomyces sp.
BGC0000844	0.68	Other	clavulanic acid	Streptomyces clavuligerus
BGC0001164	0.67	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001163	0.66	Polyketide	1-heptadecene	Moorea producens 3L
BGC0002631	0.66	RiPP	myxarylin	Pyxidicoccus fallax
BGC0000122	0.66	Polyketide	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0002000	0.65	Polyketide	setomimycin	Streptomyces aurantiacus JA 4570
BGC0000892	0.65	Other	caryoynencin	Burkholderia gladioli BSR3
BGC0002514	0.64	Polyketide	julichrome Q6.6	Streptomyces sampsonii

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 94427:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (ccmal - ccmal) + (Me-ccmal - ccmal) + (ccmal) + (ohmal) + (ccmal - Me-ccmal - ccmal) + (mal)
Non-colinear ordering:: ANH11409.1 + ANH11410.1 + ANH11412.1 + ANH11413.1 + ANH11414.1 + ANH11415.1

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ANH11402.1

PKS_AT (31..288): pk

ATSignature: (unknown)

No matches above 50%

minowa: CHC-CoA

Prediction, score:

CHC-CoA: 14.0
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Malonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

ANH11404.1

AMP-binding (17..413): X

nrpys: bMe-Asp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DCFFFALITK
Nearest Stachelhaus code(s):: bMe-Asp DCFFFALITK (100% 8Å match); Ala DCFFFALITK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

ANH11405.1

AMP-binding (21..420): X

nrpys: 3S-Abu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DYYPTTQRIK
Nearest Stachelhaus code(s):: 3S-Abu DYYPTTQRIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ANH11409.1

PKS_AT (653..947): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%; Propionyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 205.3
inactive: 103.4
Methoxymalonyl-CoA: 73.6
Methylmalonyl-CoA: 64.6
Propionyl-CoA: 35.0
Isobutyryl-CoA: 27.3
Ethylmalonyl-CoA: 26.7
2-Methylbutyryl-CoA: 22.3
Acetyl-CoA: 20.9
Benzoyl-CoA: 19.2
fatty_acid: 17.5
CHC-CoA: 15.7
trans-1,2-CPDA: 13.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (2346..2631): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.0
inactive: 96.6
Methoxymalonyl-CoA: 64.8
Methylmalonyl-CoA: 54.5
Isobutyryl-CoA: 26.3
Propionyl-CoA: 25.7
Acetyl-CoA: 20.7
Ethylmalonyl-CoA: 17.1
Benzoyl-CoA: 13.5
fatty_acid: 9.7
CHC-CoA: 1.5
2-Methylbutyryl-CoA: 1.1
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

ANH11410.1

PKS_AT (567..862): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 215.1
Ethylmalonyl-CoA: 207.6
Methoxymalonyl-CoA: 179.6
trans-1,2-CPDA: 142.7
2-Methylbutyryl-CoA: 140.3
Propionyl-CoA: 112.6
Malonyl-CoA: 112.4
CHC-CoA: 102.6
Benzoyl-CoA: 85.0
Isobutyryl-CoA: 77.1
Acetyl-CoA: 46.5
3-Methylbutyryl-CoA: 41.5
fatty_acid: 40.0
inactive: 30.7

PKS_AT (2274..2569): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 87.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.8
inactive: 117.9
Methoxymalonyl-CoA: 69.6
Methylmalonyl-CoA: 51.6
Propionyl-CoA: 32.3
Isobutyryl-CoA: 25.3
Benzoyl-CoA: 21.5
Ethylmalonyl-CoA: 19.6
Acetyl-CoA: 17.5
CHC-CoA: 12.0
trans-1,2-CPDA: 8.1
2-Methylbutyryl-CoA: 4.2
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

ANH11412.1

PKS_AT (589..876): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 79.2%; Methylmalonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 194.2
inactive: 96.4
Methoxymalonyl-CoA: 64.3
Methylmalonyl-CoA: 62.3
Propionyl-CoA: 32.1
Isobutyryl-CoA: 29.1
Ethylmalonyl-CoA: 23.7
Benzoyl-CoA: 22.9
Acetyl-CoA: 22.3
2-Methylbutyryl-CoA: 14.6
fatty_acid: 13.4
CHC-CoA: 10.5
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

ANH11413.1

PKS_AT (555..831): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 210.4
inactive: 117.8
Methoxymalonyl-CoA: 52.9
Methylmalonyl-CoA: 44.0
Isobutyryl-CoA: 30.7
Propionyl-CoA: 18.7
Benzoyl-CoA: 16.8
fatty_acid: 11.7
Ethylmalonyl-CoA: 10.9
2-Methylbutyryl-CoA: 1.2
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
Acetyl-CoA: -2.5

ANH11414.1

PKS_AT (571..854): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 224.8
inactive: 114.6
Methoxymalonyl-CoA: 66.4
Methylmalonyl-CoA: 58.3
Propionyl-CoA: 33.9
Isobutyryl-CoA: 23.6
Acetyl-CoA: 22.9
Benzoyl-CoA: 21.1
Ethylmalonyl-CoA: 19.9
2-Methylbutyryl-CoA: 11.0
CHC-CoA: 10.5
fatty_acid: 10.3
trans-1,2-CPDA: 9.2
3-Methylbutyryl-CoA: 0.0

PKS_AT (2243..2531): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Methoxymalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 91.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 212.3
Ethylmalonyl-CoA: 206.9
Methoxymalonyl-CoA: 168.7
2-Methylbutyryl-CoA: 135.7
trans-1,2-CPDA: 121.8
CHC-CoA: 109.8
Malonyl-CoA: 104.9
Propionyl-CoA: 101.7
Isobutyryl-CoA: 77.0
Benzoyl-CoA: 68.0
3-Methylbutyryl-CoA: 38.5
Acetyl-CoA: 37.4
fatty_acid: 34.2
inactive: 22.5

PKS_AT (3908..4192): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.8
inactive: 84.2
Methoxymalonyl-CoA: 65.6
Methylmalonyl-CoA: 50.2
Propionyl-CoA: 39.1
CHC-CoA: 22.4
Acetyl-CoA: 18.9
Isobutyryl-CoA: 11.6
trans-1,2-CPDA: 11.0
Benzoyl-CoA: 10.7
Ethylmalonyl-CoA: 6.6
2-Methylbutyryl-CoA: 5.9
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

ANH11415.1

PKS_AT (556..830): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 79.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 208.3
inactive: 104.6
Methoxymalonyl-CoA: 57.9
Methylmalonyl-CoA: 42.3
Propionyl-CoA: 29.9
Acetyl-CoA: 22.9
Isobutyryl-CoA: 13.6
Benzoyl-CoA: 12.9
fatty_acid: 12.0
Ethylmalonyl-CoA: 10.5
2-Methylbutyryl-CoA: 8.7
trans-1,2-CPDA: 4.9
CHC-CoA: 0.0
3-Methylbutyryl-CoA: 0.0