LOCUS       BGC0001781              2902 bp    DNA     linear   BCT 24-SEP-2016
DEFINITION  Planomonospora sphaerica DNA, contig: NODE_12_L315350_C40, whole
            genome shotgun sequence.
ACCESSION   BGC0001781
VERSION     BGC0001781
KEYWORDS    .
SOURCE      Planomonospora sphaerica
  ORGANISM  Planomonospora sphaerica
            Bacteria; Actinobacteria; Streptosporangiales; Streptosporangiaceae;
            Planomonospora.
COMMENT     Annotated using prokka 1.11 from http://www.vicbioinformatics.com.
            This strain was obtained from JCM
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 04:22:54
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 158349
            Orig. end    :: 161251
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..120
                     /gene="sphA"
                     /locus_tag="PS9374_04799"
     CDS             1..120
                     /codon_start=1
                     /gene="sphA"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /locus_tag="PS9374_04799"
                     /product="putative lasso peptide precursor"
                     /protein_id="GAT69128.1"
                     /transl_table=11
                     /translation="MRKRYESPALTCEGTFFEKTKGLPIGWWIERPSGWYFPI"
     subregion       1..2902
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="RiPP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..2902
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            364..2169
                     /gene="sphC"
                     /locus_tag="PS9374_04800"
     CDS             364..2169
                     /codon_start=1
                     /gene="sphC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 99.5; E-value: 3.1e-30)"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_046929684.1"
                     /locus_tag="PS9374_04800"
                     /product="putative lasso peptide modification enzyme"
                     /protein_id="GAT69129.2"
                     /sec_met_domain="Asn_synthase (E-value: 2e-30, bitscore:
                     94.7, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLSEIVAASSWFVVVPDRDESKWIMSKLQESVGDLAVVEHASGR
                     PWILGRWKDDELLLREDGTRHGVRVALMGEYPGNEATKDIVQRAVQNGQGIDRIDLRLR
                     GSFHLIASVGNEVRAQGTASGFRRLYYNRTGKIRFVGSRADVVAWLSNSEMSEERLLLR
                     LLNYVPWPFSAQPVWAGVRGVPPGSYIRLASDGSVRETAWWSPPPPRASLREGALTLRS
                     ELAAAVELRTAPGTGVSADLAGLDSTALCSILSETAGSVHALTVEGADPRNDDLHWAEL
                     TASALPELKHVVIGTDQAPTMFDGLISGAYDFDGPCSMVINRSAFLATVTRSLSHGSAL
                     HFSGFGGDELLIRSPAHLHRLMRNNPAVAFRLAREFSARYSWPLIRMARLLAASESYAD
                     WLRGASGRLLDQHFGPSYPMLGWDLPLCMPDWFMPEAARTVSRFIRQVAENASPLSTDQ
                     GLHSTMHHIISGAAVAREFQLISERAGLRLAVPYYDDRVMEACLAVRPEDVLTPRRYKP
                     LLVEAMKGVMPAKALSRSTKGGANQEYAMGLERNQDQIMTLIEESKLVQLGLIDARKLR
                     QASMVSTPNPLLVNTIGVECWLRSL"
     gene            2214..2471
                     /gene="sphB1"
                     /locus_tag="PS9374_04801"
     CDS             2214..2471
                     /codon_start=1
                     /gene="sphB1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /locus_tag="PS9374_04801"
                     /product="putative lasso peptide modification enzyme"
                     /protein_id="GAT69130.1"
                     /sec_met_domain="PF05402 (E-value: 4.4e-17, bitscore: 50.4,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1.8e-26,
                     bitscore: 80.2, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFSLKPGVSLAETENGTVLLDEGAAKYWSLNESGTVIFRVLLEG
                     GSLDDVVTALIEEFEIDEATALHDARRLTDDFKAAGLIRR"
     gene            2468..2902
                     /gene="sphB2"
                     /locus_tag="PS9374_04802"
     CDS             2468..2902
                     /codon_start=1
                     /gene="sphB2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:Prodigal:2.6"
                     /inference="similar to AA sequence:RefSeq:WP_050997427.1"
                     /locus_tag="PS9374_04802"
                     /product="putative lasso peptide modification enzyme"
                     /protein_id="GAT69131.1"
                     /sec_met_domain="PF13471 (E-value: 9e-28, bitscore: 84.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAFGEEAHDLRQLSYRRRLGVRIAIGVAFFLARRSPAQMRAVFT
                     LLRKGARAASYGEARQAREAVVTRSLICESRRGCLPRSIAAALVCRLGGTWPTWCVGVG
                     TAPPFGAHAWIEADGRMVEEEEPSSHYRKLITIGPLGEKR"
ORIGIN
        1 atgcgaaagc gctacgagtc gcccgctctg acatgcgaag gtaccttttt cgagaagaca
       61 aagggtctcc cgatcggctg gtggatcgag aggccttccg gctggtactt cccgatctga
      121 gtcggaggat tgattccgct gacagggtca gatccccggg aggctttccc gcattgatcg
      181 gagtttcctg acccttttgt tgcgcctgca ggtgggagtg gggtggagaa ttgatttccc
      241 gctcccacct gttcgtccct gtgcgcatcc cgttcccgtg ggtgcagggc tcttcagtct
      301 tccagcggag tgccggggcg cccgagaaat ggttctggaa gacatccgca tgcgaggacg
      361 gcagtgaagc tttccgagat agtggccgct tcatcctggt tcgtcgtggt tcctgaccgg
      421 gatgagtcca aatggatcat gtcgaagttg caggaatcgg tgggagacct ggccgtcgtc
      481 gagcacgctt ccggccgccc atggatactc gggcggtgga aggacgacga gctccttctc
      541 cgcgaggacg gtacgagaca tggagtacgt gtcgcgttga tgggcgagta ccccgggaac
      601 gaggcgacca aggacatcgt tcaacgtgcc gtccagaacg ggcagggaat cgatcgtatc
      661 gatctccgcc tgcgcggcag cttccacctc atcgcatcgg ttgggaacga ggtacgggcc
      721 cagggaaccg catccgggtt ccgccggctc tactacaacc gcacgggaaa gatccggttc
      781 gtcggcagtc gcgctgacgt cgtcgcctgg ctttcgaact cggagatgag cgaagagcgt
      841 ctcctgctga gactgctgaa ctatgttccg tggcctttct ccgcccaacc ggtgtgggca
      901 ggagtccgcg gagtgccacc gggaagctac atcaggctgg catctgacgg gagcgtgcgc
      961 gaaacggcct ggtggtcgcc gccgccaccg agggcctctc tccgggaggg agctctcacg
     1021 ctgcggagcg agctggcggc ggcagtggaa ctcagaacgg cacccggtac cggggtctcc
     1081 gccgacctgg ccggactcga ttccaccgca ctgtgttcca tcctgtcgga aaccgcaggc
     1141 tccgttcacg cactcacggt cgaaggggcg gacccgcgga acgacgacct ccattgggcc
     1201 gagctcaccg cttccgccct cccggagctc aaacacgtgg tgatcggcac cgatcaggct
     1261 cccacgatgt tcgacgggct gatcagcggt gcctacgact tcgacggccc atgctccatg
     1321 gtgatcaatc ggagtgcttt cctggccacc gtcaccaggt ccttgtccca tgggtcggcg
     1381 ctgcactttt ccggtttcgg gggcgatgaa ctcctcattc ggtctccggc acacctccat
     1441 cgcctgatgc ggaacaatcc cgccgtggcg tttcgcctgg cccgtgagtt ctcggcgcgg
     1501 tattcctggc ctctcatacg catggccagg ctcctcgccg cctcggagtc ctacgccgac
     1561 tggctgcggg gtgcgtccgg gcggctgctt gatcagcact tcgggccgtc ctacccgatg
     1621 ctcggctggg atctcccgct gtgcatgccg gactggttca tgccggaggc ggcccgtacg
     1681 gtttcgcggt tcatccggca ggttgcggag aacgcctccc cgctgtccac cgatcagggc
     1741 ctgcactcga ccatgcatca catcatctcc ggggcagcgg tcgcgcgaga gttccagctc
     1801 atcagcgaac gggccggatt gcggcttgcc gtgccgtatt acgacgaccg ggtcatggag
     1861 gcgtgtctgg ccgttcgccc ggaagatgtg ctgacgcctc ggcgatacaa gcctctgctc
     1921 gtcgaagcca tgaaaggcgt tatgccggcg aaggccctga gcaggagtac caaaggcgga
     1981 gcgaatcaag agtatgccat gggcttggag cggaaccagg accagatcat gacgctgatc
     2041 gaggagtcga aactggtaca gctcgggctg atcgacgccc ggaaactgcg ccaggcgtcc
     2101 atggtctcca cgccgaatcc tctcctggtc aacaccatcg gtgtggagtg ctggctacgg
     2161 tccctttgac aagagcgatt gatctgaaaa tcactgttca cacggggatg gaaatggatt
     2221 tttctctgaa gccgggtgta tcgctggccg aaacggagaa cggtacggtc ctgctcgatg
     2281 aaggagccgc caagtactgg agcctcaacg agagcggcac tgtcattttc agggtcctcc
     2341 tcgaaggagg gagccttgat gacgtggtga ccgccctgat cgaggagttc gagatagacg
     2401 aagccacggc gctccatgac gctcgacggt tgacggacga cttcaaggcc gcagggctga
     2461 tcaggagatg acggcgttcg gagaagaggc gcacgacctc cggcagcttt cctacaggcg
     2521 gcggctgggt gtacgaatcg cgatcggtgt ggccttcttc ctggccagga gatctccggc
     2581 gcagatgcgt gccgtcttca ctctcctgcg taagggcgca cgtgccgcat cctacgggga
     2641 ggcacgccag gccagggaag ccgtcgtcac ccgtagcctg atctgcgaga gtcgtcgagg
     2701 gtgcctaccc cggtcgatcg ccgcggcatt ggtctgccgc ctcggtggaa catggccgac
     2761 gtggtgcgtc ggagtcggta ccgctccacc gttcggcgcc cacgcgtgga tcgaagcaga
     2821 tggaaggatg gtcgaggagg aggagcccag cagccattac cgcaagctga tcaccattgg
     2881 tccgctgggc gagaagcgct ga
//
